Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,447 A→C 16.3% A4A (GCA→ACC RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4470A→C16.3% 79.6 ‑2.3 0.0177A4A (GCA→GCCRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/19):  ref base (78/70):  total (88/89)
Fisher's exact test strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

CCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGC  >  NC_014034/2155347‑2155547
                                                                                                    |                                                                                                    
cccGGCCGGGGTCCCCCTCCCCTTCTGATCCCCTCGGGCGCCGTCCCGGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                      <  2:1593872/101‑1 (MQ=255)
 cggtcctcgGTCGCCCTCACCTTCTGATTCCCTCGGGCCCCGTCACCGTCCCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                     <  1:1992282/94‑1 (MQ=255)
  cGGCCGCGGTCCCCCTCCCCTTCTGATGCCCGCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                                    <  2:2056344/101‑1 (MQ=255)
   ggCCGCGGTCCCCCTCACCTTCTGATGCCCTCGGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                   <  1:1599369/101‑1 (MQ=255)
   ggCCGCGGCCGCCCTCCCCTTCTGATGCCCGCGGGCGCCGTCACCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                   <  1:4128763/101‑1 (MQ=255)
        gggccGCCCCCCCCTCCTCTTGCCTGCGGCCCCCGCCCCCGGCGCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                              <  1:1188311/97‑1 (MQ=255)
        cgGTCCCCCCCCCCTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                              <  1:3048440/101‑1 (MQ=255)
         ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                             <  1:4180335/101‑1 (MQ=255)
         gcccccccTCCCCTTCTGATGCCCCGGGGCGCCGTCCCCGTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                             <  2:3201501/96‑1 (MQ=255)
           ccccccTCCCCTTCTGATCCCCCCGGGCGCCGCCCCCGCCCCCCCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                           <  1:517783/98‑1 (MQ=255)
            cGCCCCCCCTTTCTGATCCCCTCGGGCGCCGCCCCCGGCGCCGCCCGCGGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                          <  2:3356230/101‑1 (MQ=255)
             gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGACACCGCCCCCCCGGCGGCAACGCGGGAAAGGGCAGCAGCATGAGCGATGCAAGGGGACACGTag                                                                                         >  2:506512/1‑101 (MQ=255)
              cccTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                        <  1:2745070/101‑1 (MQ=255)
              cccTCCCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                        <  2:1175997/101‑1 (MQ=255)
              cccTCCCCTTCTGAGCCCCTGGGCCGCGGCCACCCTCCCCCCCGGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                        <  2:1878866/101‑1 (MQ=255)
              cccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                        >  1:4062930/1‑101 (MQ=255)
                ccccccTTCTGATCCCCTCGGGCCCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                      <  1:1043147/97‑1 (MQ=255)
                 cccccTTCTGATCCCCTGGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                                     <  1:3792844/98‑1 (MQ=255)
                  cACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                    >  2:243054/1‑101 (MQ=255)
                      ttCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                                >  1:1687522/1‑101 (MQ=255)
                       tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                               >  2:1283786/1‑101 (MQ=255)
                         tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                             >  1:6111/1‑101 (MQ=255)
                         tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                             >  2:2647920/1‑101 (MQ=255)
                         tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                             >  2:2348429/1‑101 (MQ=255)
                           aTGCCCTGGGGCCCCGCCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                           <  2:3089802/101‑1 (MQ=255)
                             gCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                         >  2:603609/1‑101 (MQ=255)
                             ccccTCGGGCGCCGTCCCCGCCCCCGCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                         <  2:370273/100‑1 (MQ=255)
                              cccTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                        >  1:1568435/1‑101 (MQ=255)
                              cccCCGGGCGCCGTCCCCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                        <  1:342610/101‑1 (MQ=255)
                                    ggCGCCGCCCCCGTCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                  <  2:39119/101‑1 (MQ=255)
                                     gcgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                                 >  2:3220082/1‑101 (MQ=255)
                                           tcaccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                           >  2:2820582/1‑101 (MQ=255)
                                            caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCgg                                                          >  2:1729015/1‑100 (MQ=255)
                                            caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                          >  1:192955/1‑101 (MQ=255)
                                             ccctcccccccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                         <  1:2956785/91‑1 (MQ=255)
                                              cgttcgccgcccggGGGGCGCCAAGCCGCGAAAGGGGAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCGCGACGATGCCATCTGGGGCGGGGCCTTca                                                        <  2:3475655/95‑1 (MQ=255)
                                                gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                      >  2:840324/1‑101 (MQ=255)
                                                ccccccccccgcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                      <  2:827554/94‑1 (MQ=255)
                                                 tcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                                     >  2:301628/1‑101 (MQ=255)
                                                      gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGGCGTCACCACGATg                                                >  2:267955/1‑101 (MQ=255)
                                                       cccgccGGGCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                               <  2:4153661/101‑1 (MQ=255)
                                                        ccgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                              >  2:2874784/1‑101 (MQ=255)
                                                        ccgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                              <  2:4001591/101‑1 (MQ=255)
                                                         cgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                             <  1:2505706/101‑1 (MQ=255)
                                                            cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                          >  2:354705/1‑101 (MQ=255)
                                                             gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                         >  2:3789401/1‑101 (MQ=255)
                                                                  gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                    <  2:3496777/99‑1 (MQ=255)
                                                                    atctcgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                  <  2:3634651/97‑1 (MQ=255)
                                                                    aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                  >  2:984011/1‑101 (MQ=255)
                                                                    aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                  >  1:2180979/1‑101 (MQ=255)
                                                                     aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                 >  1:416624/1‑101 (MQ=255)
                                                                     aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                 <  1:695063/101‑1 (MQ=255)
                                                                     aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                 <  1:2423696/101‑1 (MQ=255)
                                                                      gCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                                <  2:678858/101‑1 (MQ=255)
                                                                      gCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAAAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                                <  1:268000/101‑1 (MQ=255)
                                                                       ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCgg                               >  2:1089276/1‑101 (MQ=255)
                                                                       ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                               >  1:3560941/1‑101 (MQ=255)
                                                                          cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                            >  2:543223/1‑101 (MQ=255)
                                                                           gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                           >  1:813513/1‑101 (MQ=255)
                                                                            aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          >  1:3274361/1‑101 (MQ=255)
                                                                            aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          <  2:2606885/101‑1 (MQ=255)
                                                                             aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGggcggcg                         >  2:2690397/1‑100 (MQ=255)
                                                                             aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                         >  2:255528/1‑101 (MQ=255)
                                                                              aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                        >  1:2083659/1‑101 (MQ=255)
                                                                                ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt                      <  2:2969634/101‑1 (MQ=255)
                                                                                gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                      <  1:4179696/92‑1 (MQ=255)
                                                                                 gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                     >  2:3569839/1‑101 (MQ=255)
                                                                                  gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                    <  2:3314312/94‑1 (MQ=255)
                                                                                  cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgg                    >  2:2751358/1‑100 (MQ=255)
                                                                                   aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg                   <  2:1306654/95‑1 (MQ=255)
                                                                                   aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                   >  2:430638/1‑101 (MQ=255)
                                                                                   aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                   >  2:2180712/1‑101 (MQ=255)
                                                                                   aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                   <  1:2648126/101‑1 (MQ=255)
                                                                                    ggATGATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                  >  2:3582362/1‑101 (MQ=255)
                                                                                    ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                  >  2:3161475/1‑101 (MQ=255)
                                                                                    aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                  >  1:3722990/6‑101 (MQ=255)
                                                                                    aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                  <  1:48332/96‑1 (MQ=255)
                                                                                     gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc                 <  1:3140995/101‑1 (MQ=255)
                                                                                     aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc                 <  1:2137272/97‑1 (MQ=255)
                                                                                      aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                >  1:967259/1‑101 (MQ=255)
                                                                                       tCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAt               >  1:3953812/1‑101 (MQ=255)
                                                                                       aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt               >  2:3817785/3‑101 (MQ=255)
                                                                                        aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt              <  1:1829486/100‑1 (MQ=255)
                                                                                        aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt              <  1:1044687/100‑1 (MQ=255)
                                                                                        aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt              >  1:2891517/2‑101 (MQ=255)
                                                                                         aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc             <  1:4025019/101‑1 (MQ=255)
                                                                                         aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc             <  2:1711026/101‑1 (MQ=255)
                                                                                         aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc             <  1:1420867/101‑1 (MQ=255)
                                                                                          tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg            <  2:2307370/101‑1 (MQ=255)
                                                                                          tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg            <  2:201665/101‑1 (MQ=255)
                                                                                          tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg            <  2:1977537/101‑1 (MQ=255)
                                                                                            accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc          <  1:3544135/99‑1 (MQ=255)
                                                                                            accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc          <  1:2151677/99‑1 (MQ=255)
                                                                                            aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc          >  2:6146/1‑101 (MQ=255)
                                                                                             gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc         <  1:836526/101‑1 (MQ=255)
                                                                                             ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCNTGATGTGGCATTCGAcc         >  2:746756/2‑101 (MQ=255)
                                                                                              cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg        <  1:2875003/101‑1 (MQ=255)
                                                                                              cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg        <  2:409275/101‑1 (MQ=255)
                                                                                              cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg        >  1:185526/1‑101 (MQ=255)
                                                                                                   aaaGGGGCC.CCTAGAGCTCGTCGATGCCCTCTGGGGGGGGGGCCGCACCCCCATGGGGGGGGGGCCCCCCCGGGGGGCGATGTGGGCTTCGGCCGCggggg  >  2:2962125/2‑98 (MQ=255)
                                                                                                    |                                                                                                    
CCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGC  >  NC_014034/2155347‑2155547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG