Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,450 G→A 16.2% R5R (AGG→AGA RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4500G→A16.2% 78.7 ‑2.7 ‑0.1180R5R (AGG→AGARCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/19):  ref base (82/68):  total (93/87)
Fisher's exact test strand distribution p-value = 6.67e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.65e-01

Only 100 of 187 total aligned reads displayed.

GGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGG  >  NC_014034/2155350‑2155550
                                                                                                    |                                                                                                    
ggCCGCGGTCCCCCTCACCTTCTGATGCCCTCGGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                      <  1:1599369/101‑1 (MQ=255)
     gggccGCCCCCCCCTCCTCTTGCCTGCGGCCCCCGCCCCCGGCGCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                                 <  1:1188311/97‑1 (MQ=255)
     cgGTCCCCCCCCCCTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                                 <  1:3048440/101‑1 (MQ=255)
      ggTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                                <  1:4180335/101‑1 (MQ=255)
       gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                               >  1:4050195/1‑101 (MQ=255)
         cccccTCCCCTTCGGATGCCCCCGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                             <  1:2759610/99‑1 (MQ=255)
          gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                            >  1:4153923/1‑101 (MQ=255)
           ccccccccTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                           <  1:4110374/95‑1 (MQ=255)
           cccTCCCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                           <  2:1175997/101‑1 (MQ=255)
             ccccccTTCTGATCCCCTCGGGCCCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                         <  1:1043147/97‑1 (MQ=255)
             cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                                         >  1:2146572/1‑101 (MQ=255)
             cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAAGGGCCTCGTAGAGc                                                                                         >  2:2819695/1‑101 (MQ=255)
              tCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                                        >  1:3090032/1‑101 (MQ=255)
               ccccTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                       <  1:2158583/99‑1 (MQ=255)
               cACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                       >  2:243054/1‑101 (MQ=255)
               cACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                       >  2:3238396/1‑101 (MQ=255)
                cccTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                      <  2:3342103/100‑1 (MQ=255)
                  cTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                                    >  1:526955/1‑101 (MQ=255)
                   ttCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                                   >  1:1687522/1‑101 (MQ=255)
                    tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                                  >  2:1283786/1‑101 (MQ=255)
                      tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                                >  2:2348429/1‑101 (MQ=255)
                      tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                                >  2:2647920/1‑101 (MQ=255)
                          gCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                            >  2:603609/1‑101 (MQ=255)
                           cccTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                           >  1:1568435/1‑101 (MQ=255)
                            ccTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCt                                                                          >  2:3211237/1‑101 (MQ=255)
                               cGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                                       >  2:2570574/1‑101 (MQ=255)
                                 ggCGCCGCCCCCGTCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                     <  2:39119/101‑1 (MQ=255)
                                  gcgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                                    >  1:201703/1‑101 (MQ=255)
                                   cgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcgg                                                                   >  2:1851147/1‑101 (MQ=255)
                                      cgtcaccgtcacCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggggc                                                                >  1:78736/1‑101 (MQ=255)
                                       gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                               >  1:827635/1‑101 (MQ=255)
                                         caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                             >  1:846853/1‑101 (MQ=255)
                                          ccctcccccccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                            <  1:2956785/91‑1 (MQ=255)
                                           cgttcgccgcccggGGGGCGCCAAGCCGCGAAAGGGGAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCGCGACGATGCCATCTGGGGCGGGGCCTTca                                                           <  2:3475655/95‑1 (MQ=255)
                                             gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                         >  2:2997949/1‑101 (MQ=255)
                                             gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                         >  2:840324/1‑101 (MQ=255)
                                             gcccccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                         <  2:514284/97‑1 (MQ=255)
                                             ccccccccccgcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                         <  2:827554/94‑1 (MQ=255)
                                              cccccccccgccGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                                        <  2:2707371/95‑1 (MQ=255)
                                                accgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCCCCACg                                                      >  2:2874651/1‑101 (MQ=255)
                                                 ccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                                     >  1:672984/1‑101 (MQ=255)
                                                   gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGGCGTCACCACGATg                                                   >  2:267955/1‑101 (MQ=255)
                                                    cccgccGGGCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                                  <  2:4153661/101‑1 (MQ=255)
                                                     ccgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                                 <  2:4001591/101‑1 (MQ=255)
                                                      cgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                                <  1:2505706/101‑1 (MQ=255)
                                                         cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                             >  2:354705/1‑101 (MQ=255)
                                                               gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                       <  2:1695898/101‑1 (MQ=255)
                                                               gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                       <  2:3496777/99‑1 (MQ=255)
                                                                 aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                     >  2:984011/1‑101 (MQ=255)
                                                                  aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                    <  1:3220321/101‑1 (MQ=255)
                                                                   gCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                                   <  2:678858/101‑1 (MQ=255)
                                                                   gCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                                   >  1:234424/1‑101 (MQ=255)
                                                                   gCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAAAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                                   <  1:268000/101‑1 (MQ=255)
                                                                    ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCgg                                  >  2:1089276/1‑101 (MQ=255)
                                                                    ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                                  >  1:3560941/1‑101 (MQ=255)
                                                                     cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGAGGCCGTCACCACGATGGCGGGGGCGCTCGcagat                                 >  1:3634904/1‑97 (MQ=255)
                                                                        gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                              >  1:348982/1‑101 (MQ=255)
                                                                         aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                             <  2:2606885/101‑1 (MQ=255)
                                                                          aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                            >  2:255528/1‑101 (MQ=255)
                                                                          aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                            <  1:1851267/101‑1 (MQ=255)
                                                                             ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt                         <  1:423630/101‑1 (MQ=255)
                                                                             ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt                         <  2:2969634/101‑1 (MQ=255)
                                                                              gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                        >  2:3069510/1‑101 (MQ=255)
                                                                              gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                        >  2:3569839/1‑101 (MQ=255)
                                                                               cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                       <  1:2105713/101‑1 (MQ=255)
                                                                                aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg                      <  1:398526/95‑1 (MQ=255)
                                                                                aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg                      <  2:1306654/95‑1 (MQ=255)
                                                                                aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                      >  2:430638/1‑101 (MQ=255)
                                                                                aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                      >  2:2180712/1‑101 (MQ=255)
                                                                                aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                      <  1:2648126/101‑1 (MQ=255)
                                                                                aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCCCCGGGCGGCTGAtgtg                      >  1:50327/1‑101 (MQ=255)
                                                                                 ggATGATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                     >  2:3582362/1‑101 (MQ=255)
                                                                                 ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                     >  2:3161475/1‑101 (MQ=255)
                                                                                 aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                     <  2:3732997/96‑1 (MQ=255)
                                                                                   aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                   >  1:2451222/4‑101 (MQ=255)
                                                                                    aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt                  >  2:3817785/3‑101 (MQ=255)
                                                                                     aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                 <  1:1829486/100‑1 (MQ=255)
                                                                                     aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                 <  1:1044687/100‑1 (MQ=255)
                                                                                     aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                 >  1:2891517/2‑101 (MQ=255)
                                                                                      aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAttg                >  2:555857/1‑100 (MQ=255)
                                                                                      aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                <  1:3321350/101‑1 (MQ=255)
                                                                                      aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                <  2:1721045/101‑1 (MQ=255)
                                                                                      aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                <  1:1420867/101‑1 (MQ=255)
                                                                                       tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg               <  2:2307370/101‑1 (MQ=255)
                                                                                       tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg               <  2:201665/101‑1 (MQ=255)
                                                                                       tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg               <  1:3824183/101‑1 (MQ=255)
                                                                                       tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg               <  2:1977537/101‑1 (MQ=255)
                                                                                        gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa              <  2:3637237/101‑1 (MQ=255)
                                                                                        gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa              <  1:4175109/101‑1 (MQ=255)
                                                                                         aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc             >  2:6146/1‑101 (MQ=255)
                                                                                          ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGGGCTGGCCGGGCGCCTGATGTGGCATTCGAcc            <  1:3753183/100‑1 (MQ=255)
                                                                                          ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCNTGATGTGGCATTCGAcc            >  2:746756/2‑101 (MQ=255)
                                                                                           cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg           <  2:409275/101‑1 (MQ=255)
                                                                                            gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa          >  2:381157/1‑101 (MQ=255)
                                                                                                cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg      >  1:1855685/1‑101 (MQ=255)
                                                                                                cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg      <  2:3437129/101‑1 (MQ=255)
                                                                                                aaaGGGGCC.CCTAGAGCTCGTCGATGCCCTCTGGGGGGGGGGCCGCACCCCCATGGGGGGGGGGCCCCCCCGGGGGGCGATGTGGGCTTCGGCCGCggggg     >  2:2962125/2‑98 (MQ=255)
                                                                                                 aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc     >  2:1884295/1‑101 (MQ=255)
                                                                                                  aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg    <  1:2884948/101‑1 (MQ=255)
                                                                                                  aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCg    >  1:3731935/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGG  >  NC_014034/2155350‑2155550

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG