Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,712 C→G 16.1% Q93E (CAG→GAG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,7120C→G16.1% 69.9 ‑4.0 0.0190Q93E (CAG→GAG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (22/9):  ref base (74/85):  total (96/94)
Fisher's exact test strand distribution p-value = 1.77e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

GGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCTTTGAG  >  NC_014034/2155612‑2155811
                                                                                                    |                                                                                                   
   gATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAgg                                                                                                  <  1:2928349/101‑1 (MQ=255)
     tcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTg                                                                                                <  1:2048426/101‑1 (MQ=255)
     tcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTg                                                                                                >  2:3608149/1‑101 (MQ=255)
      catcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgc                                                                                               >  2:384065/1‑100 (MQ=255)
      catcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTgg                                                                                               >  1:1675355/1‑101 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgct                                                                                              >  1:2002381/1‑99 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgcg                                                                                              >  2:123602/1‑99 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGGGCCCAGGTGgc                                                                                              >  2:778801/1‑101 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                                              <  1:929818/101‑1 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                                              >  1:2646844/1‑101 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                                              <  2:1568326/101‑1 (MQ=255)
        tcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctg                                                                                             <  1:652514/101‑4 (MQ=255)
         cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgc                                                                                            <  2:2190755/101‑5 (MQ=255)
         cGGCGAGGCGGTGGCGGCTTATCTCGGGCTGGGGCGGACGGTCACCCCCGGCGCCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgc                                                                                            <  2:677430/101‑5 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggctgct                                                                                           >  2:941006/1‑94 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                                           >  1:2192635/1‑96 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                                                                                          <  1:4169441/101‑1 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                                                                                          <  1:2832483/101‑1 (MQ=255)
            cGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGa                                                                                         >  2:2529159/1‑101 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGat                                                                                        >  1:3006879/1‑100 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGACCGCGCGGCGCCCAGGTGGCGCTGAc                                                                                        >  2:1069192/1‑101 (MQ=255)
              aGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatg                                                                                       >  2:3910321/1‑99 (MQ=255)
               ggCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGagca                                                                                      >  2:521761/1‑98 (MQ=255)
                gCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCg                                                                                     >  2:1485180/1‑101 (MQ=255)
                gCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCg                                                                                     <  1:1155161/101‑1 (MQ=255)
                gCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGGGGCGCTGACCCg                                                                                     >  2:1635404/1‑101 (MQ=255)
                 cGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagg                                                                                    >  1:1000899/1‑96 (MQ=255)
                  ggcggcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCgcg                                                                                   >  2:2389623/4‑99 (MQ=255)
                  ggTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCggg                                                                                   >  2:2775657/1‑101 (MQ=255)
                   gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                                                  >  1:3349284/1‑94 (MQ=255)
                   gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                                                  >  2:3696725/1‑94 (MQ=255)
                   gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                                                  <  2:1927928/101‑8 (MQ=255)
                   gTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGt                                                                                  >  2:414620/1‑101 (MQ=255)
                   gTGGCGGCTTATTTCGAGCGCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGt                                                                                  <  2:2868432/101‑1 (MQ=255)
                    tGGCGGCTTATTTCGAGCCCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtc                                                                                 <  1:1545246/101‑1 (MQ=255)
                     ggcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatca                                                                                >  2:3001449/1‑92 (MQ=255)
                     ggcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatca                                                                                >  1:1078125/1‑92 (MQ=255)
                      gcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatcat                                                                               >  1:3389758/1‑91 (MQ=255)
                         gcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGCTGGCGCTGACCCGGGTcttctt                                                                            >  2:620491/1‑101 (MQ=255)
                           ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                                          >  2:1714093/1‑101 (MQ=255)
                           ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                                          >  2:3199359/1‑101 (MQ=255)
                            tATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTca                                                                         <  1:3211476/101‑1 (MQ=255)
                             aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGCCCCGGGTCTTCTTTcac                                                                        >  1:998980/1‑101 (MQ=255)
                                tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                                     <  1:1828169/101‑1 (MQ=255)
                                  gAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCAcaacaa                                                                   >  1:1472789/1‑101 (MQ=255)
                                     cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                                                <  2:3746253/101‑1 (MQ=255)
                                      tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                                               >  1:3740978/1‑101 (MQ=255)
                                      tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                                               <  1:342634/101‑1 (MQ=255)
                                       cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                                              <  1:1108849/101‑1 (MQ=255)
                                            ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                                         >  2:2755027/1‑101 (MQ=255)
                                            ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                                         <  2:2030079/101‑1 (MQ=255)
                                             gACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                                        >  1:1617097/1‑101 (MQ=255)
                                              aCCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgaga                                                       >  1:2712359/1‑101 (MQ=255)
                                              aCCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgaga                                                       <  1:2158254/101‑1 (MQ=255)
                                                cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                     <  1:3878236/101‑1 (MQ=255)
                                                 gTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATga                                                    <  1:1729134/101‑1 (MQ=255)
                                                  tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                                   >  1:3770770/1‑101 (MQ=255)
                                                  tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                                   <  1:1701485/101‑1 (MQ=255)
                                                  tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                                   <  2:1353186/101‑1 (MQ=255)
                                                   gACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacg                                                  >  1:2100977/1‑101 (MQ=255)
                                                     ccaccGGCGTCGTCTCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacgac                                                <  2:1107925/101‑1 (MQ=255)
                                                     ccaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacgac                                                <  1:2392148/101‑1 (MQ=255)
                                                      caccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACa                                               >  2:353408/1‑101 (MQ=255)
                                                       cccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                                              <  2:496006/100‑1 (MQ=255)
                                                       accGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                                              >  1:1267026/1‑101 (MQ=255)
                                                       accGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                                              >  2:1290685/1‑101 (MQ=255)
                                                          ggCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACt                                           <  1:1337862/101‑1 (MQ=255)
                                                          ggCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACt                                           <  2:3740724/101‑1 (MQ=255)
                                                           gCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTg                                          <  1:2382855/101‑1 (MQ=255)
                                                            cgtcgtCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTgg                                         >  1:4101230/1‑101 (MQ=255)
                                                             gtcgtcGCAACGCTCGCCTATCTCGGTCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGc                                        <  1:3552128/101‑1 (MQ=255)
                                                                  cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCggggc                                   >  1:1188680/1‑99 (MQ=255)
                                                                  cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcg                                   >  2:1002054/1‑100 (MQ=255)
                                                                  cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcc                                   <  2:2592704/101‑1 (MQ=255)
                                                                   gCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcg                                  >  1:1367477/1‑101 (MQ=255)
                                                                   gCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCcg                                  <  2:1792706/101‑1 (MQ=255)
                                                                   gCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCcg                                  <  1:791292/101‑1 (MQ=255)
                                                                        gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcggggccg                             >  1:2894104/1‑94 (MQ=255)
                                                                        gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAg                             <  2:3033202/101‑1 (MQ=255)
                                                                          tCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCt                           <  1:2943098/101‑1 (MQ=255)
                                                                           cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCgcgcgcacg                          >  1:3274115/1‑96 (MQ=255)
                                                                           cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTg                          <  2:1970449/101‑1 (MQ=255)
                                                                              cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCgg                       <  2:1746789/101‑1 (MQ=255)
                                                                                aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCgggcgggtggcggc                     >  2:2871619/1‑91 (MQ=255)
                                                                                  ctcGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGTCGACAGACTGGCGGGGGGCGCCGCCGCggggcc                   >  1:3607616/1‑97 (MQ=255)
                                                                                   tcGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCg                  <  2:4199315/101‑1 (MQ=255)
                                                                                    cGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGGGGCGGGTCCgg                 >  2:3382063/1‑101 (MQ=255)
                                                                                      gCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGgc               <  2:1616988/101‑1 (MQ=255)
                                                                                      gCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGgc               <  2:2011967/101‑1 (MQ=255)
                                                                                       cccGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCGGCgggccccgcgg              >  2:3880360/1‑92 (MQ=255)
                                                                                       cccGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCAGCCGCCGCTCCcgggg              >  2:189754/1‑99 (MQ=255)
                                                                                         cgcgcgGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCt            <  1:469244/101‑1 (MQ=255)
                                                                                          gcgcgGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTc           <  1:2529343/101‑1 (MQ=255)
                                                                                           cgcgGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCGGCGGCGCCCGGCGCTCg          >  2:4029010/1‑101 (MQ=255)
                                                                                            gcggcgCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCgg         <  1:3756699/101‑1 (MQ=255)
                                                                                              ggcgCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCt       <  1:608257/101‑1 (MQ=255)
                                                                                               gcgcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGGGCGCCGCCGCGGCCCCCCgcgccccgcct      >  1:3531306/1‑94 (MQ=255)
                                                                                                cgcCCAGGTGGCGCTGACCCGGGTCTTCTTNCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCttt     <  1:2318957/101‑1 (MQ=255)
                                                                                                 gcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCTCGCCGCTGCGGCCCCCGgcgcgccgggttt    >  2:3476482/1‑92 (MQ=255)
                                                                                                   ccAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCTTTGAg  <  2:1188586/101‑1 (MQ=255)
                                                                                                    |                                                                                                   
GGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCTTTGAG  >  NC_014034/2155612‑2155811

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG