Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 221,973 G→C 1.6% P228P (CCC→CCG)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034221,9730G→C 1.6% 5.7 ‑0.6 0.0570P228P (CCC→CCG) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/7):  ref base (281/276):  total (286/284)
Fisher's exact test strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 1481 total aligned reads displayed.

CCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCG  >  NC_014034/221873‑222082
                                                                                                    |                                                                                                             
  gggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgcggg                                                                                                             <  2:1979377‑M1/20‑1 (MQ=255)
   ggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggg                                                                                                            <  1:3643383‑M1/21‑2 (MQ=255)
         tgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtc                                                                                                      >  2:909301‑M1/75‑101 (MQ=255)
          gggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcg                                                                                                     >  1:773111‑M1/74‑101 (MQ=255)
           ggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgg                                                                                                    <  2:597445‑M1/29‑1 (MQ=255)
           ggatcggtcgggtccgtcggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgg                                                                                                    <  2:3457229‑M1/29‑1 (MQ=255)
             ttCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggt                                                                                                  >  1:2775774/1‑101 (MQ=255)
             atcggtcggatccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggat                                                                                                  <  1:2512305‑M1/31‑3 (MQ=255)
              tcgggcgggtccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtc                                                                                                 >  1:4083713‑M1/70‑101 (MQ=255)
               cggtcggatccgtgggatcgggcgggtccgtgggatcggtcgggtccgtcggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtcc                                                                                                >  2:116411‑M1/69‑101 (MQ=255)
                   cggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcgggtccgtgg                                                                                            <  1:465808‑M1/37‑1 (MQ=255)
                     ggtccgtggggtccgtcggatccgtgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtggga                                                                                          >  2:3928895‑M1/63‑101 (MQ=255)
                      gtccgtgggatcggtcgggtccgtcggatcggtgggatccgtgggatcggtcgggtccgtgggatcggtcgggtccgtcggatcggtcgggtccgtgggat                                                                                         >  1:1837309‑M1/62‑101 (MQ=255)
                        tggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGgtcgggtccgtgggatcggtcgggtccgtgggatcg                                                                                       <  1:3527921/101‑1 (MQ=255)
                        ccgtgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcg                                                                                       >  2:1353812‑M1/60‑101 (MQ=255)
                           tgggatcggtcgggtccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtc                                                                                    <  1:3854001‑M1/45‑1 (MQ=255)
                           tCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCgggtccgtgggatcggtcggggccgtgggatcggtc                                                                                    >  1:999414/1‑101 (MQ=255)
                            cAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGggtccgtgggatcggtcgggtccgtgggatcggtcg                                                                                   <  2:2646024/101‑1 (MQ=255)
                              ggtccgtcggatccgtgggatcggtcggatccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGa                                                                                 <  1:3284898‑M1/48‑1 (MQ=255)
                               atcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAt                                                                                <  1:3219942‑M1/49‑1 (MQ=255)
                                tcggtcgggtccgtgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtc                                                                               >  1:1426479‑M1/52‑98 (MQ=255)
                                 gggtcgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                                              >  1:1324002‑M1/51‑101 (MQ=255)
                                   gCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGt                                                                            >  2:1317439/1‑101 (MQ=255)
                                     cgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTgg                                                                          <  2:2733061‑M1/55‑1 (MQ=255)
                                     cgggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgg                                                                          <  1:2846248‑M1/55‑1 (MQ=255)
                                     cgggtccgtgggatcggtcggatccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCGGTgg                                                                          <  1:3929568‑M1/55‑1 (MQ=255)
                                      gggtccgtgggatcggtcggatccgtgggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGCCGGATCCGTggg                                                                         <  2:3256810‑M1/56‑1 (MQ=255)
                                       ggtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGa                                                                        <  1:1005158‑M1/57‑1 (MQ=255)
                                        gtCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                       <  1:2966243/100‑1 (MQ=255)
                                                   cggtcgggtccgtgggatcggtcgggtccgtgGCATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                            >  1:3599530‑M1/33‑101 (MQ=255)
                                                   cggtcgggtccgtcggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                            <  1:114800‑M1/69‑1 (MQ=255)
                                                    ggtcgggtccgtggggtcggtcgggtccgtgGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                                                           >  2:2546326‑M1/32‑101 (MQ=255)
                                                          atccgtgggatcggtcgggtccgtgGGATCGGTCGGGCCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGTGATCGGTCGGGCCCGTGGgac                                                     <  1:2363281‑M1/76‑2 (MQ=255)
                                                           tccgtggtatcggtcgggtccgtgGGAGCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                    >  2:3767083‑M1/25‑101 (MQ=255)
                                                            ccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                   >  1:1960310‑M1/24‑101 (MQ=255)
                                                            ccgtgggatcggtcggatccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                   <  2:4092789‑M1/78‑1 (MQ=255)
                                                             cgtgggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcgg                                                  <  1:3477459‑M1/79‑1 (MQ=255)
                                                              gtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggt                                                 <  1:564177‑M1/80‑1 (MQ=255)
                                                               tCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtccgtg                                                <  2:2440845/101‑2 (MQ=11)
                                                               ggggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                >  1:2722509‑M1/21‑101 (MQ=255)
                                                                cGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                                               <  2:2827289/101‑1 (MQ=255)
                                                                     gCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATcc                                          >  2:2075183/1‑101 (MQ=255)
                                                                     cggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATcc                                          >  1:2421040‑M1/15‑101 (MQ=255)
                                                                     cggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATcc                                          <  2:3617721‑M1/87‑1 (MQ=255)
                                                                     ccGTGCGATCGGTCGCATTCGTGGGGTCCATCCGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATcg                                          <  2:2606316/100‑2 (MQ=14)
                                                                      cctGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCATGGGAGCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATTGGGTGGGTCCg                                         >  2:52963/3‑101 (MQ=14)
                                                                       gtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCCGTCGGATCCGt                                        <  2:3526656‑M1/89‑1 (MQ=255)
                                                                        tGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                       >  2:434983/1‑101 (MQ=255)
                                                                        tGGGATCGGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGtc                                       <  2:3157492/101‑2 (MQ=11)
                                                                            gtccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGGTCGGTAGGAGCCGTGGGAt                                   >  2:3098978/8‑101 (MQ=11)
                                                                             tcggtcggATCGGTCGGGACCGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                  <  1:1698036/101‑1 (MQ=255)
                                                                             gccgtgggattggtggggtccgtgggatcggtcgggtccgtgGTATCGGTCGGTTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtc                                  <  1:124173/95‑1 (MQ=11)
                                                                              ccgttgagtcggtcgggtccgtgggagcggtcgggtccgtgggaTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcg                                 <  2:1453927/93‑1 (MQ=14)
                                                                              ccgtcggATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTGGGAtcg                                 >  1:3380109/3‑101 (MQ=11)
                                                                                gtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                               >  1:944536‑M1/4‑101 (MQ=255)
                                                                                gtcggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggt                               >  2:1837189/1‑101 (MQ=14)
                                                                                 tggggcgcggcgggtgcgtggggtcggtcgggtccgtgggatcgggCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcggtc                              <  1:1503635/91‑1 (MQ=14)
                                                                                 tgggatcggtcgggtccgtggggtcggtcggggccgtgGGGTCCGTCGGATGCGGGGGATCGGTAGGAGCCGTGGGGTGGGTCGGGTCCGTGGGAacggag                              >  2:3987546/3‑99 (MQ=2)
                                                                                 tgggatcggtcgggtccgtgggatcggtcgggtccgtgGGACCGGTCGGGTCCGTGGGGTCGGACGGCTCCGTGGGATCGGCCGGAGCCGTggcggcgtcc                              >  1:675750/3‑93 (MQ=11)
                                                                                 tgGGATCGGTCGGTTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                              >  2:368240‑M1/3‑101 (MQ=255)
                                                                                 tgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                              >  1:1888505‑M1/3‑101 (MQ=255)
                                                                                 tgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGtcggtc                              <  1:1765829‑M1/99‑1 (MQ=255)
                                                                                 tgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGgatccgtggggtccgtg                              >  1:2047255‑M1/3‑94 (MQ=255)
                                                                                  gggatcggtcggatccgtgggatcggtcggatccgtaGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGAtcggtcg                             <  2:123405/100‑1 (MQ=11)
                                                                                  cggGTCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                             <  1:2149827/101‑1 (MQ=11)
                                                                                   gggtccgtcggatccgtgggatcggtcggatccgtgggatcgGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTGGGATCCGTGGGAtcggtcgg                            >  1:2905311/7‑101 (MQ=14)
                                                                                    ggtccgtcgggtccgtgggatcggtcgggtccgtcggatcgGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCggg                           <  1:629591/96‑1 (MQ=14)
                                                                                    gatcggtcgggtccgtgggatcggtcgggtccgtggGGTCCGTCGGATCCGTGGGATCGGGCGGAGCCGTGGGGTCGGGCCGGTCCGTGGGAACGGGCggg                           >  2:1551034/1‑101 (MQ=11)
                                                                                    gatcggtcgggtccgtgggatcggtcgggtccgtggGATCGGTCGGATCCGTGGGGTCGGTGGGGTCCGTTGGATCGGTCGGGTCCGCGGGaacagggggg                           >  2:2960032/1‑92 (MQ=11)
                                                                                     atcggtcgggtccgtcggatcggtcgggtccgtgggATCGGTCGGGTCCGTCGGATCGGTGGGGTCCGGGGGATCGGTCGGGTCCGGGGGATCGGCCGGGt                          >  2:817408/1‑101 (MQ=11)
                                                                                      tcggtcgggtcctttgggtccgtcgggtccgtgggaTCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                         <  2:1051962/101‑1 (MQ=11)
                                                                                       cggtcgggtccgtggggtccgtcggatccgtgggatCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                        <  1:2112022/101‑1 (MQ=11)
                                                                                       cggtcgggtccgtgggatcggtcgggtccgtcggatCGGTGGGATCCGTGGGATCGGTCGGGCCCGTGGGATCGGTCGGGTCCGTTGGATCGGTCGGGGcc                        >  1:2631777/1‑101 (MQ=11)
                                                                                        ggtcgggtccgtgggatcggtcgggtccgtcggatcGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCCGTCGGATCGGGCGGGCCCg                       >  1:2309912/1‑101 (MQ=11)
                                                                                        ggtcggatccgtgggatcggtcgggtccgtgggatcGGTCGGATCCGTGGGATCGGTCGGGCCCGTGGGGCCGGTGGGGTCCGCGGCACCGCTCGGCTCCg                       >  2:2241074/1‑101 (MQ=1)
                                                                                         gtcgggtccgtgggatcggtcgggtccgtgggatcgGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGt                      >  1:3716526/1‑101 (MQ=14)
                                                                                         gtcggatccgtgggatcggtcggatccgtaggatcgGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                      >  1:3073678/1‑101 (MQ=11)
                                                                                          tgggatccgtgggatcggtcgggtccgtgggatcggtcggGTCCGTCGGATCGGGCGGGTCCGTGGGGTCGGTCGGGGCCGTGGGGTCGGTCGGGTCCGtg                     >  2:1761211/6‑100 (MQ=11)
                                                                                          tcgggtccgtgggatcggtcgggtccgtgggatcggTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCggggctgtg                     >  2:3044153/1‑95 (MQ=11)
                                                                                          tcgggtccgtcggatcggtcgggtccgtgggatcggTCGGGTCCGTCGGGTCGGGGGGGTCCGGGGGGTCGGTCGGGGCCGTGGGATCGGTCTGGGCCGtc                     >  2:189880/1‑100 (MQ=11)
                                                                                           cgggtccgtgggatcggtcgggtccgtggggtccgtCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGtgg                    >  1:3421742/1‑99 (MQ=11)
                                                                                           cggatccgtgggatcggtcggatccgtgggatcggtCGGATCCGTGGGATCGGGCGTGCCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGGTccctgc                    >  1:225022/1‑97 (MQ=14)
                                                                                            gggtccgtgggatcggtcgggtccggcgggtcgggcGGGGCGGTGCGGTGGGGCGGGGCCGTGGGCTCGGTGGGGTCCGTGGGATCGGTCCGGTCTGTGgg                   >  2:1655471/1‑101 (MQ=2)
                                                                                            ggggccgtgggatcggtcgggtccgtgggatcggtcGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGgg                   <  1:873027/101‑1 (MQ=11)
                                                                                            ggatccgtggggtccgtcgggtccgtgggatcggtcggGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGGTCCGTGgg                   >  1:3780025/4‑101 (MQ=11)
                                                                                            ggatccgtgggatcggtcggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGCTCGGGCGGGCCCGTGgg                   >  1:1632282/4‑101 (MQ=11)
                                                                                             ggtccgtgggatcggtcgggtccgtgggatcggtcgGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTggga                  >  1:4189783‑M2/1‑97 (MQ=255)
                                                                                             gggccgtgagatcggtcgggtccgggggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGa                  <  1:3133541/98‑1 (MQ=11)
                                                                                             gagccgtgggatcggtcggggccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGa                  <  1:2583916/98‑1 (MQ=11)
                                                                                              gtccgtggggtccggcgggtccgtgggattgttcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAt                 <  1:400333/101‑1 (MQ=11)
                                                                                              gtccgtgggaccggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                 <  2:739681/101‑1 (MQ=11)
                                                                                              atccgtgggatcggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCGGTGGGGTCGGTCGGGTCCGGGGGATCGGTGGGGTCCGTGggtt                 >  1:1074696/2‑99 (MQ=14)
                                                                                               tccgtgggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatc                >  2:2143892‑M2/1‑95 (MQ=255)
                                                                                               tccgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatc                <  2:3855465‑M2/101‑7 (MQ=255)
                                                                                               tccgtgggatcggtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGGCCGTGgggtg                >  2:2302524/1‑98 (MQ=14)
                                                                                               gccgtgggatcggtcggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGAtc                <  1:1564583/100‑1 (MQ=11)
                                                                                                 cgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATACGTGGGTCCCGTCGAATCCGTGGGAacgg              >  2:651080/1‑101 (MQ=11)
                                                                                                   tgggatcggtcggttccgtgggatcggccggGTCCGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc            <  2:2362369/101‑1 (MQ=11)
                                                                                                    gggatctgttgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg           <  2:3366311/101‑1 (MQ=11)
                                                                                                    gggatcggtcggatctgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGTGAGCGTTCGGATCCGTAGGATCGGTCGGGTCCGTAGGAtcggtcg           <  2:2890874/101‑1 (MQ=14)
                                                                                                    |                                                                                                             
CCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCG  >  NC_014034/221873‑222082

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG