Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,066 A→G 2.8% D197D (GAT→GAC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0660A→G 2.8% 17.8 ‑1.5 0.0579D197D (GAT→GACRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/7):  ref base (249/311):  total (261/318)
Fisher's exact test strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 756 total aligned reads displayed.

GGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGG  >  NC_014034/221966‑222166
                                                                                                    |                                                                                                    
ggtccgtgggatcggtcgggtccgtgggatcggtcgGGTCCGTGGGATAGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAACGGGCGGGGCCGTGGGa                                                                                                      >  2:1207572/1‑101 (MQ=11)
gatccgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGCCCGGTCCGTGGGa                                                                                                      >  2:2693003/3‑101 (MQ=11)
 gtccgtgggatcggtcggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGAt                                                                                                     <  1:3048018/101‑1 (MQ=11)
 gtccgtcggatcggtcgggtccgtgggatcggtcggGTCCGTCGGATCGGTGGGGTCCGTGGGATCGCTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAt                                                                                                     >  1:3631230/1‑101 (MQ=11)
  tccgtgggatcggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTTGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGAtc                                                                                                    <  1:3524097/101‑1 (MQ=14)
  tccgtgggatcggtcggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGAtc                                                                                                    <  2:3939650/101‑1 (MQ=11)
   ccgttggatcggtcgggtccgcgggatcggtcggGTCCGTGGTATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                                                   <  2:3740911/101‑1 (MQ=11)
   ccgtgggatcggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGATCCGTAGGGtcg                                                                                                   >  2:877414/1‑101 (MQ=11)
   ccgtgggagcgttcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                                                                   <  2:1428986/101‑1 (MQ=11)
    cgtgggatcggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGATCCGGAGGGTCGGTCGGGTCCGGAGGAtcgg                                                                                                  >  2:3207433/1‑101 (MQ=11)
    cgtgggacccggcggggccgtgggatctgtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcgg                                                                                                  <  1:2005147/101‑1 (MQ=11)
     gtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGGGCCGTGGGAgcggt                                                                                                 >  1:2058500/1‑101 (MQ=11)
     gggggatcggtcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGAtcatt                                                                                                 <  2:2470955/99‑4 (MQ=11)
      tgggatcggtcggggccgtgggatcggtcggGTCCGTGGGGTCGGTCGGAGCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGCGGAtcggac                                                                                                >  1:2488738/1‑99 (MQ=11)
      ggggatcggtcgggtccgtgggatccgtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                                                <  2:2832539/100‑1 (MQ=14)
      ggggatcggtcggatccctgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                                                                <  2:2609777/100‑1 (MQ=14)
       gggatctgttgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                                                                                               <  2:3366311/101‑1 (MQ=11)
        gggtccgtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGAtcggtcgg                                                                                              >  1:812182/7‑101 (MQ=11)
        ggagcggtcgggtccgggggatcggtcggATCCGTGGGGTCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtcgg                                                                                              <  1:933833/101‑1 (MQ=11)
         gatcggtcgggtccgtgggatcggtcggATCCGTG.........GGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggg                                                                                    >  1:2714035/1‑101 (MQ=11)
         gatcggtcggatccgtaggatcgggcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtaggg                                                                                             <  1:3257051/101‑1 (MQ=11)
          atcggtcggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggc                                                                                            >  2:2314216/1‑100 (MQ=11)
           tcggtcgggtgcgtgggatcggtctgATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGgtcggatc                                                                                           <  2:1156622/101‑1 (MQ=11)
           tcggtcggagccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtc                                                                                           <  1:3983897/101‑1 (MQ=14)
            cggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGTATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtcc                                                                                          <  1:3577692/101‑1 (MQ=11)
            ccgtcggatccgtggggtccgtcggGTCCGTGGGGTCGGGCGGGGCCGTCGGGTCGGTCGGGGCCGAGGGATCGGTCGGGTCCGTGGGCTCGgttgggtcg                                                                                          >  2:1968412/3‑100 (MQ=2)
             ggtcgggtctgtggggtccgtcggATCCGGGGGATCGGTCGGATCCGTGGGATCGGGCGGATCCGTGGGATCGGTCGGGGCCGTGGGATCGgtcgggtccg                                                                                         <  1:2846465/101‑1 (MQ=11)
             ggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGTGGGATCGgtcgggtccg                                                                                         >  2:790534/1‑101 (MQ=11)
                cgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGGTCGGTCGGGTCCGTGGGATCGgtcgggtccgtgg                                                                                      >  2:6226/1‑99 (MQ=11)
                cgggtccgtgggatcggtcggGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCCGATCCGTGGGACCGGTCGGGTCCGTGGGATCGgtcgggtccgtgg                                                                                      >  1:2557449/1‑99 (MQ=11)
                cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCgtcgggtccgtcg                                                                                      >  2:2561610/1‑101 (MQ=11)
                cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCCTCGGATCCgtggggtccgtcg                                                                                      <  1:3117300/101‑1 (MQ=11)
                cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGGCGGGTCCGTCGGATCCgtggggtccgtcg                                                                                      >  2:4005287/1‑101 (MQ=11)
                   gtcggtggggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcggat                                                                                   <  1:4044868/101‑3 (MQ=11)
                   gtccgcgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGAGCTCATTGTATCGGTCGCGTCCGTAGGAACGggcaggtctgtggctt                                                                                   >  2:714328/1‑96 (MQ=2)
                   gcccgtgggaacggtctgTTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggt                                                                                   <  2:1724679/99‑1 (MQ=11)
                   atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                   <  2:2224466/100‑3 (MQ=255)
                   atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcggggccgtcggat                                                                                   >  1:141785/2‑99 (MQ=14)
                    tccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccggcggatc                                                                                  >  2:1546673/1‑101 (MQ=14)
                    tccgtgggatcgggcggGTCCGTGGGATCGGTCGGGTCCGTGGGCTCGGTCGGATCCGGGGGATCGGTCGGGGCCGTGGGATCGgtcggatccgcggggtc                                                                                  >  1:3842026/1‑101 (MQ=11)
                        tggggtccgtcggCTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                              <  2:4072146/101‑2 (MQ=11)
                        taggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGtggggtccgtcggggccggg                                                                              >  2:3851074/3‑99 (MQ=11)
                         gggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCTTGGGCTCGgtcggagccgtgggatgggccg                                                                             >  2:3483165/1‑93 (MQ=11)
                           gatcgggcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgggg                                                                           <  1:1628940/101‑2 (MQ=11)
                            gtcggtgggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAt                                                                          <  1:4166718/100‑1 (MQ=14)
                            gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAt                                                                          >  1:1071470/5‑101 (MQ=11)
                            gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAt                                                                          <  2:1313940/97‑1 (MQ=11)
                             tcggtgggATCGGTGGGATCGGTCGGATCCGTGGGATCGGTTGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                         <  2:1876789/101‑1 (MQ=11)
                             tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtc                                                                         <  2:3073448/101‑1 (MQ=14)
                              gtgccgtctCCGTGGTCCCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                        <  2:2913413/93‑2 (MQ=11)
                              cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcg                                                                        <  2:204522/101‑1 (MQ=14)
                              ccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtcc                                                                        <  1:1240392/99‑2 (MQ=11)
                              ccgtcggAGCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtcc                                                                        <  2:3679702/99‑2 (MQ=11)
                               ggtgtgctCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccg                                                                       <  1:1010002/94‑3 (MQ=11)
                               ggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGTGCCGtagggtccgtcgggtccgggagggccc                                                                       >  1:1188836/1‑92 (MQ=2)
                                gtcggGTCCGTGGGGTCGGCTGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggt                                                                      <  1:2312919/101‑1 (MQ=11)
                                gtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTGGGGtccgt                                                                      >  2:1626917/1‑101 (MQ=11)
                                  ggggtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcg                                                                    <  2:3808358/97‑1 (MQ=11)
                                   ggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgtggg                                                                   >  1:2428650/1‑101 (MQ=11)
                                    gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGa                                                                  <  1:1920778/101‑1 (MQ=11)
                                    gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGa                                                                  >  2:2758112/1‑101 (MQ=11)
                                      tCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTGGGATCCGTGGGGCCCGTGGGGtcggtccgggc                                                                >  2:2364739/1‑96 (MQ=11)
                                      tCCGTAGGAGCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTc                                                                <  1:1551914/101‑1 (MQ=14)
                                          tcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                                            >  2:421808/3‑101 (MQ=17)
                                          tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGACGGATCCGTGGGGGCCGTGGGATCGGTCGGGTCCGTg                                                            >  1:2943077/1‑101 (MQ=14)
                                              aTCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAt                                                        <  2:3419328/101‑1 (MQ=14)
                                              aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGAtagatcggaa                                                        >  2:651088/1‑92 (MQ=17)
                                              aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                        >  2:1259010/1‑101 (MQ=255)
                                               tcggtgggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                       <  1:526846/101‑1 (MQ=17)
                                               tcggtcgtGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                       <  2:3643130/101‑1 (MQ=17)
                                               tcggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                       <  1:1314663/101‑1 (MQ=17)
                                               tcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCAGTGGGATCGGTCGGGTCGGTCGGagt                                                       >  2:1021603/1‑99 (MQ=14)
                                               tccgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                       >  1:3344689/7‑101 (MQ=255)
                                                 ggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcgg                                                     >  1:595810/1‑101 (MQ=17)
                                                 ggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgg                                                     >  2:3285280/1‑101 (MQ=14)
                                                        tCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTc                                              >  1:637963/1‑101 (MQ=11)
                                                         gcGTGGGCTCGGTCGGGTCCGTGGGATCGGTCGGGTCGGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                             <  2:1521221/100‑1 (MQ=255)
                                                         ccGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTcc                                             <  2:570414/101‑1 (MQ=17)
                                                            tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                          <  2:949484/101‑2 (MQ=17)
                                                            tGGGATCGGTCGCTTTCGTTGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                          <  1:714404/101‑2 (MQ=14)
                                                                  cggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcg                                    >  1:2193629/1‑97 (MQ=14)
                                                                    gtcggGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcgggtccgt                                  <  1:3782669/101‑6 (MQ=14)
                                                                    gtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggt                                  >  2:1110818/1‑95 (MQ=14)
                                                                     tgggATGCGGGGGGTCGGTCGGGTCGGTAGGGTCGGTGGGTTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtg                                 <  2:2971917/99‑1 (MQ=11)
                                                                     tcggATCCGGGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                 <  1:2898056/101‑8 (MQ=11)
                                                                      cggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGGTCCGTGGGGCtggggg                                >  1:1846553/1‑101 (MQ=255)
                                                                       ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTGGGATCGGTGGGATCGGTCGGGTCGGTCGGATCGGTGGGAGCCGGgggagcgggggg                               >  2:2125286/1‑92 (MQ=2)
                                                                        ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGGCTGGTGGGa                              >  2:1912461/3‑101 (MQ=255)
                                                                           gcGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                           <  2:3517227/100‑1 (MQ=255)
                                                                           gcGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                           <  2:302983/100‑1 (MQ=255)
                                                                               gggATCGGTCGGGGCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcg                       <  1:1700110/101‑1 (MQ=255)
                                                                                        cggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGGCCGCTGt              >  2:511330/1‑101 (MQ=255)
                                                                                         gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc             >  2:177759/1‑101 (MQ=255)
                                                                                         gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGGGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGGTCGGTCGGACCGCTGTc             >  1:3931238/1‑101 (MQ=255)
                                                                                            tCCGTAGGATCGGTCGGGTTCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc          <  2:287997/101‑1 (MQ=255)
                                                                                            tCCGTAGGATCGGGCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc          <  1:3710011/101‑1 (MQ=255)
                                                                                             ccGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccg         <  1:3409708/101‑1 (MQ=255)
                                                                                               gTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc       >  1:477826/1‑101 (MQ=255)
                                                                                                 aGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgccgc     >  1:148222/1‑101 (MQ=255)
                                                                                                 aGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGGCGGACCGCTGTcgccgccgc     >  1:1122238/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGG  >  NC_014034/221966‑222166

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG