Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,075 G→A 22.0% D194D (GAC→GAT RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0750G→A22.0% 221.5 ‑3.8 0.0402D194D (GAC→GATRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (37/52):  ref base (173/139):  total (210/192)
Fisher's exact test strand distribution p-value = 2.26e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 657 total aligned reads displayed.

TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCA  >  NC_014034/221972‑222175
                                                                                                       |                                                                                                    
   gatcggtcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGCCCGTTGGACCCgtgggg                                                                                                      >  1:1298416/1‑101 (MQ=11)
    gtcggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggt                                                                                                     <  1:227749/100‑1 (MQ=11)
    atcggtcggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggc                                                                                                     >  2:2314216/1‑100 (MQ=11)
     tcggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGACCGTGGGATGGgtgggatc                                                                                                    >  2:687594/1‑101 (MQ=11)
       ggtcgggtccgtgggatcggtcggATCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGACCGgtgggatccg                                                                                                  >  1:3133258/1‑101 (MQ=11)
       ggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccg                                                                                                  <  1:358060/101‑1 (MQ=11)
       ggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccg                                                                                                  >  1:2961070/1‑101 (MQ=11)
       ggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGTGGGATCGgtcgggtccg                                                                                                  >  2:790534/1‑101 (MQ=11)
       ggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTGGGATCGgtcgggtccg                                                                                                  >  2:4073340/1‑101 (MQ=11)
       ggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgttgggtccg                                                                                                  >  2:3452863/1‑101 (MQ=14)
        gtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGAGGGATCGGTCAGGTGCGCCGGGGGGGGCGGGGCCGGGGGAGCGgtcggggccgg                                                                                                 >  2:3791727/1‑100 (MQ=2)
          cgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGGTCGGTCGGGTCCGTGGGATCGgtcgggtccgtgg                                                                                               >  2:6226/1‑99 (MQ=11)
          cgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCAGTGGGATCGGTGGGGTCCGTGGGATCGgtcgggtcagtgg                                                                                               >  2:1965490/1‑99 (MQ=11)
          cgggtccgtcggatcggtgggATCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGAGATCGgtcgggtccgtgg                                                                                               <  1:1512984/101‑3 (MQ=11)
          cgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcg                                                                                               <  2:2357379/101‑1 (MQ=11)
           ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                              <  2:1726255/98‑1 (MQ=255)
           ggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgg                                                                                              <  1:2528418/98‑1 (MQ=11)
            ggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGCTCGgtcggatccctgggc                                                                                             >  2:2655895/1‑95 (MQ=11)
             gtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCGGTGGGATGGgtcggggcggaggggc                                                                                            >  2:1503531/1‑91 (MQ=11)
              tccgtgggatcgggcggGTCCGTGGGATCGGTCGGGTCCGTGGGCTCGGTCGGATCCGGGGGATCGGTCGGGGCCGTGGGATCGgtcggatccgcggggtc                                                                                           >  1:3842026/1‑101 (MQ=11)
               ccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGgtcgggtccgtggggtcc                                                                                          >  2:2209809/1‑101 (MQ=11)
                cgtgggttgtgtaggAGCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGgtcggatccgtgggatcgg                                                                                         <  2:1307710/101‑3 (MQ=11)
                 gtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTGGGATCGGTCGCGTCCGTGGGATCCgtcgcgccggtcggcgcggt                                                                                        >  2:2846246/1‑95 (MQ=11)
                   gggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtcg                                                                                      >  2:2528234/1‑99 (MQ=11)
                   cgggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTgg                                                                                      >  2:1678750/11‑101 (MQ=11)
                   cgggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTgg                                                                                      >  1:4144948/8‑101 (MQ=11)
                    gggtccgtgggATCGGTCGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                     <  2:3310726/92‑1 (MQ=14)
                    gggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTggg                                                                                     >  1:1233736/7‑101 (MQ=11)
                    gggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTggg                                                                                     <  2:20397/95‑1 (MQ=11)
                     ggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGGGGGGTCGGGGGGa                                                                                    >  2:4022978/6‑101 (MQ=11)
                     gatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgggg                                                                                    >  1:3063623/1‑100 (MQ=11)
                      gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAt                                                                                   >  1:1071470/5‑101 (MQ=11)
                      atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAt                                                                                   <  1:2476616/101‑1 (MQ=11)
                      atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGCCGGGGCCGGCGGATCGGTCGGag                                                                                   >  2:2990382/1‑100 (MQ=11)
                       tcggtgggATCGGTGGGATCGGTCGGATCCGTGGGATCGGTTGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                  <  2:1876789/101‑1 (MQ=11)
                       tcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGAtc                                                                                  <  1:1763848/101‑1 (MQ=11)
                        tggtcggATCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcg                                                                                 <  2:4236292/100‑1 (MQ=11)
                        ccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTGGGGTCCGTCGGAtcc                                                                                 >  2:3956649/3‑100 (MQ=11)
                         ggtgtgctCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccg                                                                                <  1:1010002/94‑3 (MQ=11)
                         ggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcgg                                                                                >  1:3526513/1‑101 (MQ=11)
                         ggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGTGCCGtagggtccgtcgggtccgggagggccc                                                                                >  1:1188836/1‑92 (MQ=2)
                            cggttcgggaggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                             <  2:3388551/91‑1 (MQ=14)
                            cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCAGCGGGGTCCGTCGGGTCCGTggggctgctgg                                                                             >  2:3142353/1‑93 (MQ=14)
                            cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgg                                                                             <  1:2385522/101‑3 (MQ=11)
                             gggttcGTTGGACCGGGTGGGGCCGTGGGGGTTGTCGGATCCGTGGGATGGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcgg                                                                            <  1:405848/96‑1 (MQ=2)
                               gtCCTTGGGGTTGTTCCGGTCAGTGGGGTTCGTCCGGTCCGTGGGACCGGTCGGTTCCGTGGGATCCGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                                                                          <  2:1129180/100‑1 (MQ=9)
                                tcggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATc                                                                         <  2:3886002/95‑1 (MQ=17)
                                 cggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGGCGGATcc                                                                        >  2:495153/6‑101 (MQ=17)
                                   gtcgggtcggtgggCTCGGTGGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGt                                                                      <  2:2892340/95‑1 (MQ=17)
                                      ggATCGGTCGGGGCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                                                   <  1:140812/101‑1 (MQ=255)
                                        aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGAtagatcggaa                                                                 >  2:651088/1‑92 (MQ=17)
                                         tccgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                >  1:3344689/7‑101 (MQ=255)
                                           cgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                                                              >  1:1673797/5‑101 (MQ=255)
                                            gtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGGCCGTGGGATCGGTCGGATCCGTGGGAtcggc                                                             >  2:869231/4‑100 (MQ=255)
                                                 atCCGGGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGt                                                        <  1:3278913/100‑1 (MQ=11)
                                                  tCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                       >  2:365101/1‑101 (MQ=17)
                                                   ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGGTcc                                                      >  2:2920537/1‑101 (MQ=255)
                                                   ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTAGGATCGGTCGGGTcc                                                      >  1:288042/1‑101 (MQ=11)
                                                   ccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                      <  1:2640600/101‑1 (MQ=17)
                                                     gtcggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGt                                                    >  1:2790864/4‑101 (MQ=17)
                                                     gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGCATCGGTCGGGTCCGt                                                    >  2:1791296/1‑101 (MQ=11)
                                                      tGGGGTCGGTAGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTg                                                   <  1:3921363/101‑1 (MQ=255)
                                                      tGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTg                                                   >  2:1236862/1‑101 (MQ=255)
                                                      tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                   <  2:949484/101‑2 (MQ=17)
                                                      tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                   >  2:3215378/1‑100 (MQ=17)
                                                      tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTGGGGTCCGTg                                                   >  2:3830946/1‑101 (MQ=17)
                                                       cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTgg                                                  >  1:805346/2‑101 (MQ=17)
                                                        ggATCGGTCGGTTCCGTGGGATCCGTCGGTTCCGTGGGATCCGTCGGATCCGTTGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTagg                                                 <  2:1190167/101‑1 (MQ=14)
                                                         taccggtcggTTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggg                                                <  2:3718408/98‑1 (MQ=255)
                                                          aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                               <  1:3682918/101‑3 (MQ=14)
                                                           tcggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatc                                              <  1:1441680/101‑4 (MQ=14)
                                                            cggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGcgg                                             >  1:573024/1‑99 (MQ=255)
                                                            cggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtg                                             >  1:502607/1‑101 (MQ=255)
                                                            cggtcgcGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcgggtcc                                             <  1:683117/101‑4 (MQ=11)
                                                             ggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTGGGGTCCGTGGGATCGGTTGGGTCCGTCGGATCGGTCGGGGCAGTgggagcag                                            >  2:2756851/1‑96 (MQ=17)
                                                                cggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGCCGGACCCGTGGGATGGGTCGGGTCCGTGGGGCgggggg                                         >  2:243070/1‑101 (MQ=18)
                                                                 ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTGGGATCGGTGGGATCGGTCGGGTCGGTCGGATCGGTGGGAGCCGGgggagcgggggg                                        >  2:2125286/1‑92 (MQ=2)
                                                                  ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGGCTGGTGGGa                                       >  2:1912461/3‑101 (MQ=255)
                                                                  ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGGGGGATCGGTCGGGTCCGTGGGGCTGGTgggg                                       >  1:1870810/3‑100 (MQ=255)
                                                                   gcccGTGAGATGGGTGGGGTCCGGAGGATCCGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAt                                      <  2:1038449/99‑1 (MQ=21)
                                                                     gcGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                    <  2:3517227/100‑1 (MQ=255)
                                                                     gcGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                    <  2:302983/100‑1 (MQ=255)
                                                                     ccGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                    >  1:629102/1‑101 (MQ=255)
                                                                      cGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcgg                                   >  1:916001/1‑101 (MQ=255)
                                                                       gTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggt                                  >  1:2033560/1‑101 (MQ=255)
                                                                             tcggtgggGTGCGTGGGAGCGGGGGGGTCCGTGGGGTCCGTGGGATCGGTCGGTTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAcc                            <  1:1936364/101‑1 (MQ=21)
                                                                              cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCg                           >  1:1561000/1‑101 (MQ=255)
                                                                              cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCg                           >  2:564207/1‑101 (MQ=255)
                                                                                  ggggTCCGTAGTATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGATGt                       <  2:1597662/100‑1 (MQ=255)
                                                                                      tCCGTAGGGTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                   <  1:3777696/101‑1 (MQ=255)
                                                                                        cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgc                 >  1:2143810/1‑101 (MQ=255)
                                                                                        cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgc                 >  2:1415958/1‑101 (MQ=255)
                                                                                         gTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc                <  2:1537590/101‑1 (MQ=255)
                                                                                           aGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGGCGGACCGCTGTcgccgccgc              >  1:1122238/1‑101 (MQ=255)
                                                                                              aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg           <  1:2920756/101‑1 (MQ=255)
                                                                                                cggtcggGGCCGGCGGGTCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGAc         <  1:3987806/101‑1 (MQ=25)
                                                                                                 ggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACa        >  1:432231/1‑101 (MQ=255)
                                                                                                     gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGaac    >  2:3621909/1‑99 (MQ=255)
                                                                                                      ggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAccc   >  1:3450170/1‑101 (MQ=255)
                                                                                                       gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCa  >  2:1087797/1‑101 (MQ=255)
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TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCA  >  NC_014034/221972‑222175

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG