Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,078 C→G 4.0% T193T (ACG→ACC)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0780C→G 4.0% 22.4 ‑1.1 ‑0.1393T193T (ACG→ACC) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/10):  ref base (193/184):  total (199/194)
Fisher's exact test strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.34e-01

Only 100 of 634 total aligned reads displayed.

TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGG  >  NC_014034/221972‑222177
                                                                                                          |                                                                                                   
      cggccgggtccgtcgggtcggtgggATCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtcc                                                                                                     <  2:3043279/101‑1 (MQ=11)
       ggtcgggtccgtgggatcggtcggATCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGACCGgtgggatccg                                                                                                    >  1:3133258/1‑101 (MQ=11)
       ggtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGGTCGGGAGGATCCGCGGGTTGGGTCGGGTCCGTGGTCTCGgtcgggtccg                                                                                                    >  2:2855402/1‑101 (MQ=11)
        gtcgggtccggcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgt                                                                                                   <  1:2302692/101‑1 (MQ=11)
        gtcggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTGGGGTCCGTGGGATCGgttggatccgt                                                                                                   >  2:683040/1‑101 (MQ=1)
          cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCgtcgggtccgtcg                                                                                                 >  2:2561610/1‑101 (MQ=11)
          cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCCgggggggccgtcg                                                                                                 >  2:2040578/1‑101 (MQ=11)
           gggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggg                                                                                                >  1:3537476/1‑101 (MQ=11)
           gggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGGTCGGGCGGGTCCGTGGGATCGgtcgggtccgtcgg                                                                                                >  1:3590863/1‑101 (MQ=11)
           ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                >  1:2850568/4‑101 (MQ=255)
           ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                <  2:1726255/98‑1 (MQ=255)
           ggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgg                                                                                                <  2:3792990/98‑1 (MQ=11)
             gtcagtgggatcggtcggATCCGTGGCATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                              <  1:2545482/101‑3 (MQ=14)
             atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                              <  2:2224466/100‑3 (MQ=255)
             atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcggggccgtcggat                                                                                              >  1:141785/2‑99 (MQ=14)
              tccgtgggatcgggcggGTCCGTGGGATCGGTCGGGTCCGTGGGCTCGGTCGGATCCGGGGGATCGGTCGGGGCCGTGGGATCGgtcggatccgcggggtc                                                                                             >  1:3842026/1‑101 (MQ=11)
                 gtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTcgggtccgtcggatccgt                                                                                          >  2:3867369/1‑101 (MQ=11)
                  tggggtccgtcggCTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                         <  2:4072146/101‑2 (MQ=11)
                  tgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGtcgggtccgtcggatccggg                                                                                         >  1:3010524/1‑99 (MQ=11)
                   gggatgggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgg                                                                                        <  2:2332464/101‑1 (MQ=11)
                   cgggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTgg                                                                                        >  2:1678750/11‑101 (MQ=11)
                    ggttccgttggATCCGTGGTGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGTGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTggg                                                                                       <  2:363214/92‑1 (MQ=11)
                     ggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGGGGGGTCGGGGGGa                                                                                      >  2:4022978/6‑101 (MQ=11)
                     gatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTGGGa                                                                                      <  1:1191462/101‑1 (MQ=255)
                     gatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgggg                                                                                      >  1:3063623/1‑100 (MQ=11)
                      gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAt                                                                                     >  1:1071470/5‑101 (MQ=11)
                      atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGCCGGGGCCGGCGGATCGGTCGGag                                                                                     >  2:2990382/1‑100 (MQ=11)
                       tcggtgggATCGGTGGGATCGGTCGGATCCGTGGGATCGGTTGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                    <  2:1876789/101‑1 (MQ=11)
                       tcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGAtc                                                                                    <  1:1763848/101‑1 (MQ=11)
                       gccgtcggATCCGGGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtc                                                                                    <  1:2040716/98‑1 (MQ=11)
                        ccgtcggATCTGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtcc                                                                                   <  2:1298320/99‑2 (MQ=11)
                        ccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtcc                                                                                   <  1:1240392/99‑2 (MQ=11)
                        ccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                   >  1:2144037/3‑100 (MQ=11)
                         ggtgtgctCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccg                                                                                  <  1:1010002/94‑3 (MQ=11)
                          gtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGCtcggc                                                                                 >  2:1540964/4‑100 (MQ=14)
                          gtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTGGGGTCCGTCGGGTCCGGGGGGtcggt                                                                                 >  1:3987917/1‑101 (MQ=11)
                          gtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgt                                                                                 >  1:1564461/1‑101 (MQ=11)
                            ggggtcggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                               <  2:2659704/91‑1 (MQ=17)
                            cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCAGCGGGGTCCGTCGGGTCCGTggggctgctgg                                                                               >  2:3142353/1‑93 (MQ=14)
                            cggATCCGTGGGATCGGTCGGATCCGGGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgg                                                                               <  1:1334465/101‑3 (MQ=11)
                             gggttcGTTGGACCGGGTGGGGCCGTGGGGGTTGTCGGATCCGTGGGATGGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcgg                                                                              <  1:405848/96‑1 (MQ=2)
                             gggtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGtcgggcgg                                                                              >  2:946046/4‑101 (MQ=14)
                              gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGAtccgtgggc                                                                             >  2:3481738/1‑100 (MQ=14)
                              gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGa                                                                             >  2:2758112/1‑101 (MQ=11)
                               gtgcGTGGGGGCGGTTGGGTCCCTGGGAGCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCGGTGGGAt                                                                            <  2:1118045/98‑1 (MQ=1)
                                 cggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGGCGGATcc                                                                          >  2:495153/6‑101 (MQ=17)
                                 ccGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGTcc                                                                          >  2:214097/1‑101 (MQ=14)
                                   gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCgg                                                                        >  2:3320351/1‑100 (MQ=14)
                                   gTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGCTCGGTCGGGTCCTTCTGGTCCGTCGGAtccgtgggggcggt                                                                        >  2:1895616/1‑95 (MQ=14)
                                    tcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                                                       >  2:421808/3‑101 (MQ=17)
                                     gggctctctggggTCCGGCCGATCGGTGGGATCGGTAGGGTCCGTGGGATCGGGAGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgg                                                                      <  2:787418/91‑1 (MQ=1)
                                     aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGtcg                                                                      >  1:330519/2‑99 (MQ=14)
                                     aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGACGGGTCCGTGGGGTCGGTGAGGGCCGTCGGAGCCGTGGGCGCGGTCGGTTCAGtcg                                                                      >  2:2371413/2‑99 (MQ=2)
                                      ggATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGCCGGGTCCGTggg                                                                     >  2:1963848/1‑101 (MQ=14)
                                      ggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                                                     >  1:337397/1‑101 (MQ=255)
                                         tcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCAGTGGGATCGGTCGGGTCGGTCGGagt                                                                  >  2:1021603/1‑99 (MQ=14)
                                          ccgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                                 >  2:2492135/6‑101 (MQ=255)
                                           ggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgg                                                                >  1:2478129/1‑101 (MQ=14)
                                            gtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGCGGGAtcggt                                                               >  1:414267/4‑101 (MQ=255)
                                            gtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGGCCGTGGGATCGGTCGGATCCGTGGGAtcggc                                                               >  2:869231/4‑100 (MQ=255)
                                            gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggg                                                               >  1:3473937/1‑100 (MQ=14)
                                            ggcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggt                                                               <  2:3555294/99‑1 (MQ=14)
                                              cggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGCAGGAtcggcgg                                                             >  1:1878228/1‑98 (MQ=17)
                                               gggTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg                                                            >  2:3230250/1‑101 (MQ=17)
                                                   gcGTGGGCTCGGTCGGGTCCGTGGGATCGGTCGGGTCGGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                        <  2:1521221/100‑1 (MQ=255)
                                                   ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                        <  2:3408783/101‑1 (MQ=255)
                                                   ccGTGGGATCGGTCGGATCCGTGGGTTCGGCCGGGTCCGTGGGCTCGGTCGGATCCGTGGGGTCCGTGGGATCGCTCGGGTCCGTGGCATCGGCCGCGTcc                                                        >  1:567934/1‑101 (MQ=17)
                                                   ccGGGGGATCGGTTGGCGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                        <  2:1410263/101‑1 (MQ=14)
                                                      tGGGGTCGGTAGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTg                                                     <  1:3921363/101‑1 (MQ=255)
                                                      tGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTg                                                     >  2:1236862/1‑101 (MQ=255)
                                                      tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTGGGGTCCGTg                                                     >  2:3830946/1‑101 (MQ=17)
                                                       gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                    >  2:650021/1‑101 (MQ=255)
                                                       gggATCGGGCGGGTCCGGGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                    <  1:2888559/101‑1 (MQ=255)
                                                       cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                                    <  1:2643110/100‑1 (MQ=17)
                                                        ggATCGGTCGGTTCCGTGGGATCCGTCGGTTCCGTGGGATCCGTCGGATCCGTTGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTagg                                                   <  2:1190167/101‑1 (MQ=14)
                                                        ggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTggg                                                   >  1:1537694/1‑101 (MQ=255)
                                                        ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGTCGGTCGGGGCCGTCGGATCGGTAGGGTCCGTggg                                                   >  2:3723167/1‑101 (MQ=17)
                                                          aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                 <  1:3682918/101‑3 (MQ=14)
                                                            cggtcgcGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcgggtcc                                               <  1:683117/101‑4 (MQ=11)
                                                             ggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcgg                                              <  2:2896757/101‑6 (MQ=14)
                                                              gtcggGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcgggtccgt                                             <  1:3782669/101‑6 (MQ=14)
                                                                 ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                          >  2:613104/1‑92 (MQ=14)
                                                                 ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcgg                                          >  1:497808/1‑92 (MQ=11)
                                                                  ggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGa                                         >  2:2934183/3‑101 (MQ=255)
                                                                     ccGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                      >  1:629102/1‑101 (MQ=255)
                                                                      cGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcgg                                     >  1:916001/1‑101 (MQ=255)
                                                                       gTGGGATCGGTCGGGTCCGTAAGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCAGTGGGGCTGGTGGGAtcggt                                    >  1:2398914/1‑101 (MQ=255)
                                                                       gTGGGATCGGGCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggt                                    <  1:1478024/101‑1 (MQ=255)
                                                                        tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtc                                   >  1:785714/1‑101 (MQ=255)
                                                                             tcggtgggGTGCGTGGGAGCGGGGGGGTCCGTGGGGTCCGTGGGATCGGTCGGTTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAcc                              <  1:1936364/101‑1 (MQ=21)
                                                                                  ggggTCCGTAGTATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGATGt                         <  2:1597662/100‑1 (MQ=255)
                                                                                   gggTCGGTCGGTTCGGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                        <  2:365251/101‑1 (MQ=17)
                                                                                   gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                        >  2:177759/1‑101 (MQ=255)
                                                                                   gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGCGGGATCGGTCGGACCGCTGTc                        >  2:545688/1‑101 (MQ=255)
                                                                                      tCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                     >  1:1676213/1‑101 (MQ=255)
                                                                                           aGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGGCGGACCGCTGTcgccgccgc                >  1:1122238/1‑101 (MQ=255)
                                                                                              aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg             <  1:2920756/101‑1 (MQ=255)
                                                                                               tcggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGa            <  2:1074602/101‑1 (MQ=255)
                                                                                                   tcgggtccgtcgggtccgTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGa        <  1:430658/101‑1 (MQ=255)
                                                                                                       gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACaagcccc    >  2:3955215/1‑95 (MQ=255)
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TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGG  >  NC_014034/221972‑222177

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG