Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,317 G→A 3.6% intergenic (‑113/‑192) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3170G→A 3.6% 8.8 0.0 ‑0.1196intergenic (‑113/‑192)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (97/92):  total (101/95)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.72e-01

Only 100 of 209 total aligned reads displayed.

ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416
                                                                                                    |                                                                                                                
aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAg                                                                                                                  >  1:1527300/1‑101 (MQ=255)
   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                                                                               <  1:2785291/101‑1 (MQ=255)
    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                                                                              <  2:3036540/101‑1 (MQ=255)
    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCaa                                                                                                              <  2:2652117/101‑1 (MQ=255)
     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                                                                             <  2:1091656/101‑1 (MQ=255)
     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                                                                             >  1:3329499/1‑101 (MQ=255)
      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATGTTTGAGGCAAGa                                                                                                            <  1:2289978/101‑1 (MQ=255)
       ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                                                                           <  2:914546/101‑1 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                                                                          >  1:2916977/1‑101 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                                                                          <  1:116150/101‑1 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                                                                         >  1:59254/1‑101 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGaa                                                                                                        >  2:87558/1‑101 (MQ=255)
            gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                                   <  2:2171807/101‑1 (MQ=255)
               aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTTTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                              >  1:3603223/1‑101 (MQ=255)
               aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                              >  2:1160157/1‑101 (MQ=255)
                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                           <  2:90819/101‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                        >  1:2262979/1‑101 (MQ=255)
                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                       <  2:3723645/101‑1 (MQ=255)
                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                       >  1:136121/1‑101 (MQ=255)
                        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATc                                                                                     <  2:2965172/101‑1 (MQ=255)
                        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATc                                                                                     <  1:629049/101‑1 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                    <  2:3361288/101‑1 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                    >  1:2533207/1‑101 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATCAA...GGGG..GGCTTTCATCt                                                                                    >  1:289967/1‑101 (MQ=255)
                               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGa                                                                              <  1:2398280/101‑1 (MQ=255)
                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                             <  1:2138596/101‑1 (MQ=255)
                                 ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                            >  2:3389510/1‑101 (MQ=255)
                                 ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                            <  2:472504/101‑1 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                           >  2:1461011/1‑101 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                           >  2:2259085/1‑101 (MQ=255)
                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                          <  1:2869083/101‑1 (MQ=255)
                                     gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                        <  2:1046824/101‑1 (MQ=255)
                                     gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                        <  2:458970/101‑1 (MQ=255)
                                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                       <  2:2549880/101‑1 (MQ=255)
                                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                       <  1:4028292/101‑1 (MQ=255)
                                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                       <  1:1510934/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGTA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                     <  1:3762461/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                     <  2:1371457/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                     <  1:87582/101‑1 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                     >  1:3493532/1‑101 (MQ=255)
                                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.g                                                                   <  1:472521/101‑1 (MQ=255)
                                           ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.ggg                                                                 >  1:1677672/1‑101 (MQ=255)
                                               cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGt                                                             >  1:480985/1‑101 (MQ=255)
                                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTg                                                            <  2:3511322/101‑1 (MQ=255)
                                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTg                                                            <  2:3329656/101‑1 (MQ=255)
                                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.Tg                                                       >  2:545919/1‑101 (MQ=255)
                                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.Tg                                                       <  1:2616160/101‑1 (MQ=255)
                                                     gggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.Tgg                                                      <  1:119679/101‑1 (MQ=255)
                                                      ggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgc                                                     <  1:2230015/101‑1 (MQ=255)
                                                       gggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgc.g                                                   >  2:208618/1‑101 (MQ=255)
                                                       gggCTCTGCCCCCGTCCCGTGACGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGgc.g                                                   >  2:1753689/1‑101 (MQ=255)
                                                          ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..g                                              <  1:106933/101‑1 (MQ=255)
                                                           tctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..Gc                                             >  1:4224026/6‑101 (MQ=255)
                                                            ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..Gcc                                            <  1:1853884/97‑1 (MQ=255)
                                                              gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCag                                          >  1:3162421/3‑101 (MQ=255)
                                                                ccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..CCCagag                                        >  2:1681540/1‑101 (MQ=255)
                                                                 cccgtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAg.g                                      <  1:368489/101‑1 (MQ=255)
                                                                   cgtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                    >  1:833390/1‑101 (MQ=255)
                                                                   cgtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                    >  2:3683041/1‑101 (MQ=255)
                                                                       ccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCa                                <  2:436042/101‑1 (MQ=255)
                                                                        cgtTCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                               <  1:1813397/101‑1 (MQ=255)
                                                                        cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                               <  2:24645/101‑1 (MQ=255)
                                                                        cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                               <  2:3415444/101‑1 (MQ=255)
                                                                         gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                             <  2:1711979/101‑1 (MQ=255)
                                                                         gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                             >  2:4053323/1‑101 (MQ=255)
                                                                         gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                             >  2:1123088/1‑101 (MQ=255)
                                                                         gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                             <  1:1001720/101‑1 (MQ=255)
                                                                             cGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccc                        >  2:2919430/1‑101 (MQ=255)
                                                                             cGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccc                        >  1:995632/1‑101 (MQ=255)
                                                                              gggctccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC.GAA..GCCAGAG.GGGGGCAg.cg.c                          <  1:3059470/95‑1 (MQ=17)
                                                                              gggACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.cccc                       >  1:354411/1‑101 (MQ=255)
                                                                               gactccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC.GAA..GCCAGAG.GGGGGCAG.CG.cc                         <  2:3320767/96‑1 (MQ=17)
                                                                                gACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.cccccc                     <  2:3593020/101‑2 (MQ=255)
                                                                                 aCTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.TGGGGCAG.CG.cccccct                    <  2:4129505/101‑3 (MQ=255)
                                                                                  cTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.cccccctt                   >  1:3144277/1‑98 (MQ=255)
                                                                                   tCCCCCGAGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttc                  >  2:1624239/1‑97 (MQ=255)
                                                                                   ccccccGGGTTTTTTGGGGGAAGAGGAA...GGGG..GGTTTTCATTTTGCCAAAAAAATCCCGGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.cccccctt                   <  1:1422384/100‑4 (MQ=21)
                                                                                   ccccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttc                  <  2:2892488/100‑5 (MQ=255)
                                                                                    cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttcg                 <  2:3959378/101‑6 (MQ=255)
                                                                                     ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGC.CAAAGGCCAAGA.GGGGGCAG.CG.CCCCCtg                    >  2:1635175/1‑100 (MQ=0)
                                                                                     ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgc                 <  1:811842/101‑1 (MQ=255)
                                                                                     ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgc                 <  1:1547977/101‑1 (MQ=255)
                                                                                     ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGGGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgc                 >  1:2354331/1‑101 (MQ=255)
                                                                                      cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgcg                <  2:3190971/101‑1 (MQ=255)
                                                                                        cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGAGAAT.TGGC.CAAAGGCCAAGA.GGGGGCAG.CG.CCCCCtgacc                 >  2:1879142/1‑97 (MQ=2)
                                                                                        cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGC.CAAAGGCCAAGA.GGGGGCAG.CG.CCCCCtgccc                 >  2:1996119/1‑97 (MQ=2)
                                                                                        cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcggg              >  1:433227/1‑101 (MQ=255)
                                                                                         gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCG.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc             <  2:2916758/101‑1 (MQ=255)
                                                                                         gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc             >  1:1763632/1‑101 (MQ=255)
                                                                                         gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc             >  2:3783320/1‑101 (MQ=255)
                                                                                         gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc             <  2:410638/101‑1 (MQ=255)
                                                                                          ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggcg            <  2:2533023/101‑1 (MQ=255)
                                                                                          gaatatTGGAAGAAAGATGAG...GGGG..TTTTTTCCTCGTGCAAAAAATCTCCG.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCCC.CCCCCTTCgcgggcg            <  1:762483/99‑1 (MQ=255)
                                                                                             tatTTGCGGAAAGAGGAA...GGGGCATGTTTTCATCTTGCCCAAAATATCCC..GGGGGTGAATTGGCAAAA..GGCAAAGAGGGGGCAG.CG.CCCCCtgccccgccg            <  2:3139117/101‑10 (MQ=9)
                                                                                             tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGcc         <  1:1141657/101‑1 (MQ=255)
                                                                                               tttGAGGCAAGATGAA...GGGG..GCCTTTCATCTTGCCGAAAATACCCC.GGGGGGTGAA.TGGC.GCA..GCCAAAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCcgc       <  1:1258836/101‑1 (MQ=37)
                                                                                                ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGGG.GGGGGCAG.CGCCCCCCTTCGCGGCCGGCcgcg      >  2:1268224/1‑101 (MQ=37)
                                                                                                  gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc    <  2:1972989/101‑1 (MQ=255)
                                                                                                    ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc  >  2:370932/1‑101 (MQ=255)
                                                                                                    ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc  >  1:910587/1‑101 (MQ=255)
                                                                                                    |                                                                                                                
ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCC.GGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG