Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,332 G→T 2.1% intergenic (‑128/‑177) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3320G→T 2.1% 4.0 ‑2.1 ‑0.5187intergenic (‑128/‑177)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (93/90):  total (97/90)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.47e-01

Only 100 of 205 total aligned reads displayed.

ATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTC  >  NC_014034/2249233‑2249431
                                                                                                          |                                                                                                         
     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                      >  2:2944183/1‑101 (MQ=255)
      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                     <  2:2682658/101‑1 (MQ=255)
      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                     <  2:3572785/101‑1 (MQ=255)
       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                    <  1:82422/101‑1 (MQ=255)
       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                    <  2:3723645/101‑1 (MQ=255)
         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATc                                                                                                  <  1:629049/101‑1 (MQ=255)
          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                                 <  2:2541887/101‑1 (MQ=255)
          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                                 <  2:3361288/101‑1 (MQ=255)
          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                                 >  2:484617/1‑101 (MQ=255)
          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATCAA...GGGG..GGCTTTCATCt                                                                                                 >  1:289967/1‑101 (MQ=255)
           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                                <  1:2985079/101‑1 (MQ=255)
             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                              <  2:34017/101‑1 (MQ=255)
                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGa                                                                                           <  1:2398280/101‑1 (MQ=255)
                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                           >  2:329499/1‑101 (MQ=255)
                 tttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                          <  1:3389755/100‑1 (MQ=255)
                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                          <  1:2730742/101‑1 (MQ=255)
                  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                         <  2:641662/101‑1 (MQ=255)
                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                        >  1:1777245/1‑101 (MQ=255)
                    gggGACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                       <  1:3101496/101‑1 (MQ=255)
                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                       <  1:2869083/101‑1 (MQ=255)
                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                       <  1:1160251/101‑1 (MQ=255)
                      gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                     <  2:1046824/101‑1 (MQ=255)
                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                    <  1:4028292/101‑1 (MQ=255)
                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                    <  2:3329254/101‑1 (MQ=255)
                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                    <  1:1510934/101‑1 (MQ=255)
                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                    <  1:137626/101‑1 (MQ=255)
                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGTA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                  <  1:3762461/101‑1 (MQ=255)
                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                  <  1:87582/101‑1 (MQ=255)
                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                  <  2:1371457/101‑1 (MQ=255)
                          ccGGGGCAAGAAGGGGGCCCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                 <  2:1997830/101‑1 (MQ=255)
                           cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                >  2:2229856/1‑101 (MQ=255)
                               gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                            <  2:1527196/101‑1 (MQ=255)
                                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                           >  1:24659/1‑100 (MQ=255)
                                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                           >  1:480985/1‑101 (MQ=255)
                                 aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                          <  2:3511322/101‑1 (MQ=255)
                                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                        <  2:911974/101‑1 (MQ=255)
                                     ggggggCTCTGCCCCCGCCCCGTGCCGGGCCT.CTGCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                     <  1:3633811/100‑1 (MQ=37)
                                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                     <  1:2616160/101‑1 (MQ=255)
                                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                     >  2:1251327/1‑101 (MQ=255)
                                      gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAA.Tgg                                                                    >  1:450802/1‑101 (MQ=255)
                                        gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                                 >  2:208618/1‑101 (MQ=255)
                                        gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                                 <  1:2264758/101‑1 (MQ=255)
                                        gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                                 >  1:2206728/1‑101 (MQ=255)
                                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.gc                                                                <  2:1721042/101‑1 (MQ=255)
                                           ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..g                                                            <  1:1238753/101‑1 (MQ=255)
                                           ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..g                                                            <  1:106933/101‑1 (MQ=255)
                                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..Gc                                                           >  1:4224026/6‑101 (MQ=255)
                                            tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..Gc                                                           >  1:811589/6‑101 (MQ=255)
                                             ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..Gcc                                                          <  1:1853884/97‑1 (MQ=255)
                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCag                                                        <  2:1149245/99‑1 (MQ=255)
                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCag                                                        >  1:3162421/3‑101 (MQ=255)
                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..GCCag                                                        >  1:2243123/3‑101 (MQ=255)
                                                  cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.g                                                    <  1:368489/101‑1 (MQ=255)
                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                                  >  2:3683041/1‑101 (MQ=255)
                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                                  >  1:833390/1‑101 (MQ=255)
                                                       ccgatctcGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGc                                               <  2:425147/94‑1 (MQ=255)
                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGc                                               >  2:3872364/1‑101 (MQ=255)
                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                                             <  2:24645/101‑1 (MQ=255)
                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC.GAA..GCCAGAg.gggg                                                 >  2:2990221/8‑101 (MQ=18)
                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgc                                            <  1:1001720/101‑1 (MQ=255)
                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgc                                            >  2:4053323/1‑101 (MQ=255)
                                                          gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC.GAA..GCCAGAg.ggggg                                                >  1:144992/7‑101 (MQ=18)
                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgcg.c                                         >  1:3537406/1‑101 (MQ=255)
                                                              cGGGACT.CCTCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccc                                       <  2:459552/101‑1 (MQ=255)
                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAgatcgg                                        >  1:425204/1‑96 (MQ=255)
                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccc                                       >  1:1918090/1‑101 (MQ=255)
                                                               gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.cccc                                      >  1:1616232/1‑101 (MQ=255)
                                                                ggACT.CCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccc                                     >  1:3808942/1‑101 (MQ=255)
                                                                  aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.TGGGGCAGCG.cccccct                                   <  2:4129505/101‑3 (MQ=255)
                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.cccccctt                                  >  1:3144277/1‑98 (MQ=255)
                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttc                                 >  1:1710117/1‑97 (MQ=255)
                                                                     ccccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttc                                 <  2:2892488/100‑5 (MQ=255)
                                                                      cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttcg                                <  2:3959378/101‑6 (MQ=255)
                                                                      cccccGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.ccccccttcg                                <  2:450745/101‑6 (MQ=255)
                                                                       ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgc                                <  2:1633285/101‑1 (MQ=255)
                                                                       ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgc                                >  1:2354331/1‑101 (MQ=255)
                                                                        cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgcg                               <  2:3190971/101‑1 (MQ=255)
                                                                        cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTcgcg                               >  1:3579042/1‑101 (MQ=255)
                                                                          cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGAGAAT.TGGC.CAAAGGCCAAGA.GGGGGCAGCG.CCCCCtgacc                                >  2:1879142/1‑97 (MQ=2)
                                                                          cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC.CAAAGGCCAAGA.GGGGGCAGCG.CCCCCtgccc                                >  2:1663196/1‑97 (MQ=0)
                                                                           gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgggc                            <  2:2916758/101‑1 (MQ=255)
                                                                           gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgggc                            >  2:3783320/1‑101 (MQ=255)
                                                                           gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgggc                            <  2:410638/101‑1 (MQ=255)
                                                                            ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCgcgtgcg                           >  1:1361193/1‑101 (MQ=255)
                                                                               tatTTGCGGAAAGAGGAA...GGGGCATGTTTTCATCTTGCCCAAAATATCCC.GGGGGTGAATTGGCAAAA..GGCAAAGAGGGGGCAGCG.CCCCCtgccccgccg                           <  2:3139117/101‑10 (MQ=9)
                                                                               tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGcc                        >  2:1689121/1‑101 (MQ=255)
                                                                                atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTCCGCGGGCGGCcg                       >  1:991321/1‑101 (MQ=255)
                                                                                    gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc                   <  2:1972989/101‑1 (MQ=255)
                                                                                      ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgc                 >  2:370932/1‑101 (MQ=255)
                                                                                       gCAAGGTGGA...GGGG..GGCTCTCATCGTGCCGAAAATGCCCCGGGGGGTGAA.TGGC.GCA..GCCAGGG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcg                <  1:1681655/101‑1 (MQ=25)
                                                                                       gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcg                <  2:1112260/101‑1 (MQ=255)
                                                                                         aaGATGAG...GGGG..GGCTTTCATCTTGCCGAAAATAGCGCGGGGGGTGAA.TGGC.GCA..GCGAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcgcg              <  2:1066977/101‑1 (MQ=37)
                                                                                         aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcgcg              >  2:1837501/1‑101 (MQ=255)
                                                                                          aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc             >  2:1588134/1‑101 (MQ=255)
                                                                                            aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg           >  2:3618452/1‑101 (MQ=255)
                                                                                              gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa         >  1:735256/1‑101 (MQ=255)
                                                                                                a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc       <  2:2262820/101‑1 (MQ=255)
                                                                                                    gggg..ggTTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa      <  2:899651/101‑1 (MQ=255)
                                                                                                    gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa      >  2:1269296/1‑101 (MQ=255)
                                                                                                          ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc  >  2:2568943/1‑101 (MQ=255)
                                                                                                          |                                                                                                         
ATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTC  >  NC_014034/2249233‑2249431

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG