Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,333 G→C 3.4% intergenic (‑129/‑176) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3330G→C 3.4% 6.0 0.0 0.0187intergenic (‑129/‑176)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (93/86):  total (97/90)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

TGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCG  >  NC_014034/2249234‑2249432
                                                                                                          |                                                                                                          
tGCACAGATACCGCAGCTTGGGCACCCGGGGCATGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                            <  1:3660888/101‑1 (MQ=255)
    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                                        >  2:2944183/1‑101 (MQ=255)
     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                       <  2:3572785/101‑1 (MQ=255)
     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                       <  2:2682658/101‑1 (MQ=255)
      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                      <  2:3723645/101‑1 (MQ=255)
         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                                   <  2:2541887/101‑1 (MQ=255)
         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                                   >  2:484617/1‑101 (MQ=255)
         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                                                   <  2:3361288/101‑1 (MQ=255)
         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATCAA...GGGG..GGCTTTCATCt                                                                                                   >  1:289967/1‑101 (MQ=255)
          ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                                  <  1:2985079/101‑1 (MQ=255)
            gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                                <  2:34017/101‑1 (MQ=255)
               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAC...GGGG..GGCTTTCATCTTGCCGa                                                                                             <  1:2398280/101‑1 (MQ=255)
               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                             >  2:329499/1‑101 (MQ=255)
                cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                            <  1:2730742/101‑1 (MQ=255)
                 ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                           <  2:641662/101‑1 (MQ=255)
                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                          >  1:1777245/1‑101 (MQ=255)
                   gggGACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                         <  1:3101496/101‑1 (MQ=255)
                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                         <  1:1160251/101‑1 (MQ=255)
                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                         <  1:2869083/101‑1 (MQ=255)
                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                      <  2:3329254/101‑1 (MQ=255)
                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                      <  1:1510934/101‑1 (MQ=255)
                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                      <  1:137626/101‑1 (MQ=255)
                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                    >  1:3493532/1‑101 (MQ=255)
                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                    <  2:1371457/101‑1 (MQ=255)
                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                   <  1:472521/101‑1 (MQ=255)
                         ccGGGGCAAGAAGGGGGCCCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                   <  2:1997830/101‑1 (MQ=255)
                          cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                  >  2:2229856/1‑101 (MQ=255)
                              gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                              <  2:1527196/101‑1 (MQ=255)
                               cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                             >  1:24659/1‑100 (MQ=255)
                               cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                             >  1:602512/1‑101 (MQ=255)
                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                            <  2:3511322/101‑1 (MQ=255)
                                  gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                          <  2:911974/101‑1 (MQ=255)
                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                       >  2:1251327/1‑101 (MQ=255)
                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                       <  1:2616160/101‑1 (MQ=255)
                                       gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                                   >  2:208618/1‑101 (MQ=255)
                                       gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                                   <  1:2264758/101‑1 (MQ=255)
                                       gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.g                                                                   >  1:2206728/1‑101 (MQ=255)
                                        ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc.gc                                                                  <  2:1721042/101‑1 (MQ=255)
                                         gCTCTGCCCCCGTCCCTTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCa                                                                 <  2:1105257/101‑1 (MQ=255)
                                          ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..g                                                              <  1:106933/101‑1 (MQ=255)
                                          ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..g                                                              <  1:1238753/101‑1 (MQ=255)
                                            ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..Gcc                                                            <  1:1853884/97‑1 (MQ=255)
                                             tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCa                                                           >  1:3225845/4‑101 (MQ=255)
                                              gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCag                                                          >  1:3162421/3‑101 (MQ=255)
                                              gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..GCCag                                                          >  1:2243123/3‑101 (MQ=255)
                                                 cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.g                                                      >  1:4129767/1‑101 (MQ=255)
                                                   cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAg.ggg                                                    >  2:3683041/1‑101 (MQ=255)
                                                      ccgatctcGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGc                                                 <  2:425147/94‑1 (MQ=255)
                                                      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGc                                                 >  2:3872364/1‑101 (MQ=255)
                                                        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                                               <  2:24645/101‑1 (MQ=255)
                                                        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg                                               >  1:84139/1‑101 (MQ=255)
                                                        cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC.GAA..GCCAGAg.gggg                                                   >  2:2990221/8‑101 (MQ=18)
                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                                             >  2:4053323/1‑101 (MQ=255)
                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                                             <  1:1001720/101‑1 (MQ=255)
                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.c                                             >  2:1123088/1‑101 (MQ=255)
                                                         gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC.GAA..GCCAGAg.ggggg                                                  >  1:144992/7‑101 (MQ=18)
                                                           gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAg.cg.c                                          >  1:3537406/1‑101 (MQ=255)
                                                             cGGGACT.CCTCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccc                                        <  2:459552/101‑1 (MQ=255)
                                                             cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccc                                        >  1:1918090/1‑101 (MQ=255)
                                                             cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccc                                        >  1:995632/1‑101 (MQ=255)
                                                              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.cccc                                       >  1:1616232/1‑101 (MQ=255)
                                                                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.TGGGGCAG.CG.cccccct                                    <  2:4129505/101‑3 (MQ=255)
                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.cccccctt                                   >  1:3144277/1‑98 (MQ=255)
                                                                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttc                                  >  1:1710117/1‑97 (MQ=255)
                                                                    ccccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttc                                  <  2:2892488/100‑5 (MQ=255)
                                                                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttcg                                 <  2:3959378/101‑6 (MQ=255)
                                                                     cccccGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.ccccccttcg                                 <  2:450745/101‑6 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgc                                 <  1:811842/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgc                                 <  2:1633285/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgc                                 >  1:2354331/1‑101 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgcg                                <  2:3190971/101‑1 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAAGATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTcgcg                                >  1:3579042/1‑101 (MQ=255)
                                                                        ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgg                               >  1:82437/1‑101 (MQ=255)
                                                                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGAGAAT.TGGC.CAAAGGCCAAGA.GGGGGCAG.CG.CCCCCtgacc                                 >  2:1879142/1‑97 (MQ=2)
                                                                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC.CAAAGGCCAAGA.GGGGGCAG.CG.CCCCCtgccc                                 >  2:1663196/1‑97 (MQ=0)
                                                                         cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcggg                              >  1:636164/1‑101 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcggg                              >  1:433227/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc                             <  2:2916758/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc                             <  2:410638/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgggc                             >  2:3783320/1‑101 (MQ=255)
                                                                           ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCgcgtgcg                            >  1:1361193/1‑101 (MQ=255)
                                                                           gaatatTGGAAGAAAGATGAG...GGGG..TTTTTTCCTCGTGCAAAAAATCTCCGGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAGCCC.CCCCCTTCgcgggcg                            <  1:762483/99‑1 (MQ=255)
                                                                              tatTTGCGGAAAGAGGAA...GGGGCATGTTTTCATCTTGCCCAAAATATCCC.GGGGGTGAATTGGCAAAA..GGCAAAGAGGGGGCAG.CG.CCCCCtgccccgccg                            <  2:3139117/101‑10 (MQ=9)
                                                                              tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGcc                         >  2:1689121/1‑101 (MQ=255)
                                                                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCcg                        >  1:3698587/1‑101 (MQ=255)
                                                                                   gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAc                    <  2:1972989/101‑1 (MQ=255)
                                                                                     ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc                  >  1:910587/1‑101 (MQ=255)
                                                                                     ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgc                  >  2:370932/1‑101 (MQ=255)
                                                                                      gCAAGGTGGA...GGGG..GGCTCTCATCGTGCCGAAAATGCCCCGGGGGGTGAA.TGGC.GCA..GCCAGGG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcg                 <  1:1681655/101‑1 (MQ=25)
                                                                                       cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAGAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgc                >  1:3765924/1‑101 (MQ=255)
                                                                                        aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGAcgcgcg               >  2:1837501/1‑101 (MQ=255)
                                                                                         aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc              >  2:1588134/1‑101 (MQ=255)
                                                                                         aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc              >  1:4232831/1‑101 (MQ=255)
                                                                                           aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg            >  2:3618452/1‑101 (MQ=255)
                                                                                               a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc        <  2:2262820/101‑1 (MQ=255)
                                                                                                   gggg..ggTTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa       <  2:899651/101‑1 (MQ=255)
                                                                                                   gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa       >  2:1269296/1‑101 (MQ=255)
                                                                                                    ggg..ggCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGC.TCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg      <  1:382674/101‑1 (MQ=37)
                                                                                                    ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCCACAg      >  2:1053412/1‑101 (MQ=255)
                                                                                                         ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc   >  2:2568943/1‑101 (MQ=255)
                                                                                                          |                                                                                                          
TGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGC.GCA..GCCAGAG.GGGGGCAG.CG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCG  >  NC_014034/2249234‑2249432

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG