Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,580 G→T 100% T57K (ACG→AAG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5800G→T100.0% 736.3 195T57K (ACG→AAG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (108/87):  ref base (0/0):  total (108/87)

Only 100 of 218 total aligned reads displayed.

TCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGA  >  NC_014034/2262483‑2262677
                                                                                                 |                                                                                                 
tCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCa                                                                                                <  1:2442528/101‑1 (MQ=255)
tCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCa                                                                                                <  1:1841860/101‑1 (MQ=255)
  gCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgc                                                                                              <  1:3502355/101‑1 (MQ=255)
  gCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgc                                                                                              >  1:2591548/1‑101 (MQ=255)
    cATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccg                                                                                            >  2:1984133/1‑99 (MQ=255)
      ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                          <  1:4078691/101‑1 (MQ=255)
       ttccgatctaCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                         <  1:3318456/92‑1 (MQ=17)
       tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                         <  1:162727/101‑1 (MQ=255)
        gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                        <  1:3848893/101‑1 (MQ=255)
        gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                        >  1:949781/1‑101 (MQ=255)
        gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                        >  1:191000/1‑101 (MQ=255)
          gCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGc                                                                                      >  2:462609/1‑101 (MQ=255)
          gCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGc                                                                                      >  2:3918872/1‑101 (MQ=255)
           cTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCa                                                                                     >  1:803671/1‑101 (MQ=255)
            ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                    <  2:1605372/101‑1 (MQ=255)
             tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                   >  2:792738/1‑101 (MQ=255)
                aCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTAG.ATCGGaa                                                                               >  2:3318215/1‑100 (MQ=17)
                  cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                              <  2:3770772/101‑1 (MQ=255)
                   aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                             <  1:3052898/101‑1 (MQ=255)
                    aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                            <  2:311282/101‑1 (MQ=255)
                     tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                           >  2:3069390/1‑101 (MQ=255)
                      ggCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatc                                                                          <  2:2807641/101‑1 (MQ=255)
                       gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                         <  1:4213381/101‑1 (MQ=255)
                       gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                         <  1:2560821/101‑1 (MQ=255)
                       gccccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                         <  1:1319609/98‑1 (MQ=255)
                        cgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcca                                                                        <  1:4085270/101‑1 (MQ=255)
                         gcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcaat                                                                       >  1:657834/1‑101 (MQ=255)
                          ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                      >  1:3992601/1‑101 (MQ=255)
                           cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                     >  1:1490747/1‑101 (MQ=255)
                            agacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    >  2:367437/3‑101 (MQ=255)
                            acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    <  1:2941013/101‑1 (MQ=255)
                            acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    <  2:390693/101‑1 (MQ=255)
                            acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    <  1:603290/101‑1 (MQ=255)
                            acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                    <  1:2544257/101‑1 (MQ=255)
                              acTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccgc                                                                  >  2:3168992/1‑99 (MQ=255)
                               cTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcc                                                                 >  1:1578982/1‑101 (MQ=255)
                                tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                >  2:2272923/1‑101 (MQ=255)
                                tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                <  1:1051791/101‑1 (MQ=255)
                                tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                <  1:126126/101‑1 (MQ=255)
                                 ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                               <  2:1781277/101‑1 (MQ=255)
                                 ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                               <  1:3530889/101‑1 (MQ=255)
                                     cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGCCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACtt                                                           <  2:432163/101‑1 (MQ=18)
                                      gacccAGCACCACACCCCCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAACCGGCTCCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          <  2:4117096/98‑1 (MQ=18)
                                      gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          >  2:723182/1‑101 (MQ=255)
                                      gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          >  1:2751047/1‑101 (MQ=255)
                                      gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                          <  2:1010237/101‑1 (MQ=255)
                                        tCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGc                                                        >  1:639218/1‑101 (MQ=255)
                                           aGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtt                                                     <  1:345785/101‑1 (MQ=255)
                                            gCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACCTGGCCTTc                                                    <  2:2305339/101‑1 (MQ=18)
                                             cATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCa                                                   >  2:1057254/1‑101 (MQ=255)
                                              tcaccctCCGCCGCCAGGCGCTTGAGTTGGACGTTCTCTTCCTCAAACTGCTTCACCCGCTTGGCATCGGAAAACTCCATCCCGCCATACTTGGCCTTCAg                                                  <  2:3962267/95‑1 (MQ=12)
                                              aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                  >  2:4132463/1‑101 (MQ=255)
                                               tCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGc                                                 >  2:3509269/1‑101 (MQ=255)
                                                cacaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCt                                                >  1:737474/1‑101 (MQ=255)
                                                 ccacccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCtt                                               <  1:2266363/97‑1 (MQ=18)
                                                 acaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCtt                                               >  2:3629409/1‑101 (MQ=255)
                                                  caTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                              >  2:646352/1‑101 (MQ=255)
                                                  caTCCCCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                              <  1:3068218/101‑1 (MQ=18)
                                                   aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                             <  1:1679317/101‑1 (MQ=255)
                                                   aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                             <  1:2196926/101‑1 (MQ=255)
                                                     ccGCCACCAGGCGCTTCAGCTTGGCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                           <  2:1470949/101‑1 (MQ=18)
                                                     ccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                           <  1:1152187/101‑1 (MQ=255)
                                                          accaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                      >  1:4209016/1‑101 (MQ=255)
                                                           ccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                     <  2:1871404/101‑1 (MQ=255)
                                                            ccggCGCTTCAGCTTGGCTTTCCCTTCCTCAACCGGCTCCACCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                    <  1:2021968/99‑1 (MQ=18)
                                                            caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                    >  2:1655773/1‑101 (MQ=255)
                                                            caGGCCCTTCAGCTTGGCTTTCTCTTCCCCAAACTGCTTCAGCCGCTTGGCATCGGACAAACCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                    <  2:751460/101‑1 (MQ=18)
                                                             aGGCGCTTCAGCTTGGCGTTCCCTTCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgc                                   <  1:1168618/101‑1 (MQ=18)
                                                               gcgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgg                                 <  2:4013919/101‑1 (MQ=21)
                                                                cgcTTCAGCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                <  2:3741923/101‑1 (MQ=18)
                                                                cgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                <  2:190962/101‑1 (MQ=255)
                                                                cgcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                <  1:3629662/101‑1 (MQ=255)
                                                                   ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                             >  2:2093794/1‑101 (MQ=255)
                                                                    tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                            >  2:258136/1‑101 (MQ=255)
                                                                    tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                            <  1:2359568/101‑1 (MQ=255)
                                                                     cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                           >  1:3664322/1‑101 (MQ=255)
                                                                      aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAg                          >  2:2724807/1‑101 (MQ=255)
                                                                       gCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCTTCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                         <  2:3664069/101‑1 (MQ=21)
                                                                       gCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                         <  2:1169697/101‑1 (MQ=255)
                                                                        cTTGGCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                        <  2:3008168/101‑1 (MQ=25)
                                                                        cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                        >  1:3815311/1‑101 (MQ=255)
                                                                        cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                        >  2:2181094/1‑101 (MQ=255)
                                                                         ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                       <  2:4227675/101‑1 (MQ=255)
                                                                          tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAACGTCGCAGGGCtcaaaccg                      >  2:2368485/1‑94 (MQ=17)
                                                                          tGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                      <  1:3150798/101‑1 (MQ=21)
                                                                            gCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATg                    >  2:354569/1‑101 (MQ=255)
                                                                              tttCTCTTCCTCAAACTCCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCt                  <  2:130967/100‑1 (MQ=25)
                                                                               ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGGATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                 >  1:3748104/1‑101 (MQ=25)
                                                                                tctcTTCCTCAACCTGCTTCAGCCGCTTGGCATGGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                <  2:1438638/101‑1 (MQ=25)
                                                                                tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                >  1:864266/1‑101 (MQ=255)
                                                                                 ctctTCCTCAAACTGTTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc               <  2:3610116/101‑1 (MQ=25)
                                                                                  tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg              >  2:1548393/1‑101 (MQ=255)
                                                                                  cctTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg              <  1:4153902/100‑1 (MQ=25)
                                                                                     tCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCa           <  1:1655888/101‑1 (MQ=25)
                                                                                        tCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAg        >  1:3877571/1‑101 (MQ=255)
                                                                                         cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc       >  1:2961308/1‑101 (MQ=255)
                                                                                          aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt      >  1:2293525/1‑101 (MQ=255)
                                                                                          aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt      >  1:442311/1‑101 (MQ=255)
                                                                                          aaaCTGCTTCACCCGTTTGGCAGCGGACAAACCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt      <  2:522653/101‑1 (MQ=21)
                                                                                              tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa  >  1:2134265/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
TCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGA  >  NC_014034/2262483‑2262677

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG