Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,462,450 G→C 100% M103M (GTG→CTG)  RCAP_rcc02286 → divalent ion symporter family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,462,4500G→C100.0% 683.0 184M103M (GTG→CTG) RCAP_rcc02286divalent ion symporter family
Reads supporting (aligned to +/- strand):  new base (81/103):  ref base (0/0):  total (81/103)

Only 100 of 193 total aligned reads displayed.

TGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGGTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAC  >  NC_014034/2462352‑2462547
                                                                                                  |                                                                                                 
tGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTg                                                                                                 >  1:2802633/1‑101 (MQ=255)
tGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGGGGTCGGCGGGCTg                                                                                                 >  1:1426964/1‑101 (MQ=255)
 gACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGc                                                                                                >  1:2506312/1‑101 (MQ=255)
  aCGCGGGCCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGGCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCt                                                                                               <  1:1312752/101‑1 (MQ=255)
    gcgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCttt                                                                                             <  1:1661073/101‑1 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTc                                                                                            >  2:4075961/1‑101 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTc                                                                                            <  2:2692866/101‑1 (MQ=255)
      ggACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCg                                                                                           >  1:325932/1‑101 (MQ=255)
        aCCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAg                                                                                         >  2:521961/1‑101 (MQ=255)
         ccGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGc                                                                                        <  1:3271535/101‑1 (MQ=255)
         ccGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGc                                                                                        >  1:1380577/1‑101 (MQ=255)
            gggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGtg                                                                                     >  1:3050708/1‑101 (MQ=255)
            gggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGtg                                                                                     >  1:3837519/1‑101 (MQ=255)
             ggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGNGCTGCttnnnnnnnnnn                                                                                    >  2:414450/1‑91 (MQ=255)
             ggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGtga                                                                                    <  2:3955914/101‑1 (MQ=255)
                gATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAgagatcgga                                                                                 >  2:607049/1‑93 (MQ=255)
                gATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGGGGTCGGCGGGCTGCTTTCGAGCGtgatga                                                                                 >  1:2426740/1‑101 (MQ=255)
                  tCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAAc                                                                               >  2:3032258/1‑101 (MQ=255)
                  tCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAAc                                                                               <  2:318807/101‑1 (MQ=255)
                   cAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACa                                                                              >  1:3117906/1‑101 (MQ=255)
                    aGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACaa                                                                             >  2:2017952/1‑101 (MQ=255)
                    aGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACaa                                                                             >  1:3875554/1‑101 (MQ=255)
                     gcgcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAAt                                                                            <  2:3451610/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          <  1:3955320/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          <  1:2631856/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          <  1:1846799/101‑1 (MQ=255)
                       gcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGt                                                                          <  2:1920550/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                         <  1:1010216/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGGGATGAACAATGTc                                                                         >  2:438789/1‑101 (MQ=255)
                         gcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCa                                                                        <  2:3354445/101‑1 (MQ=255)
                         ccTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCa                                                                        <  2:1439971/100‑1 (MQ=255)
                               gcgGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGGGGTCGGCGGGCTGCTTTCGAGCGTGATGAACCATGTCACCgcgc                                                                  >  2:4115880/1‑101 (MQ=255)
                                cgGCTTCTGTGGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCt                                                                 <  2:4189376/101‑1 (MQ=255)
                                cgGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTAGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCt                                                                 >  2:778791/1‑101 (MQ=255)
                                 ggCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTg                                                                <  2:3517433/101‑1 (MQ=255)
                                 ggCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTg                                                                <  1:925082/101‑1 (MQ=255)
                                  gCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTgg                                                               >  2:237346/1‑101 (MQ=255)
                                   cTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgc                                                              >  1:2938720/1‑101 (MQ=255)
                                   cTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgc                                                              >  1:2066196/1‑101 (MQ=255)
                                    ttCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgcg                                                             >  2:3998555/1‑101 (MQ=255)
                                    ttCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgcg                                                             >  1:560695/1‑101 (MQ=255)
                                     tCTGTCGAACCCGGCGCTGCCGGTCAGCCTGTATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTAATGAACAATGTCACCGCGCTGgcgc                                                            <  2:2974780/101‑1 (MQ=255)
                                      cTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCt                                                           >  2:2400614/1‑101 (MQ=255)
                                       tGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtt                                                          <  1:1493314/101‑1 (MQ=255)
                                        gTCGATCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCttt                                                         <  2:3656761/101‑1 (MQ=255)
                                        gTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCttt                                                         <  1:1186103/101‑1 (MQ=255)
                                        gTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCttt                                                         <  1:2836832/101‑1 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                        <  2:931578/101‑1 (MQ=255)
                                          cGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTg                                                       >  2:2864691/1‑101 (MQ=255)
                                              ccaggCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGc                                                   >  1:3129075/4‑101 (MQ=255)
                                               ccGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGcc                                                  >  1:2014863/1‑101 (MQ=255)
                                               ccGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGcc                                                  <  2:1551729/101‑1 (MQ=255)
                                                cGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGccc                                                 >  1:1934428/1‑101 (MQ=255)
                                                cGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGccc                                                 <  1:4110297/101‑1 (MQ=255)
                                                  gcgcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGt                                               <  2:3830576/101‑1 (MQ=255)
                                                  gcgcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGt                                               <  1:3504461/101‑1 (MQ=255)
                                                   cgcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGtt                                              <  1:3710735/101‑2 (MQ=255)
                                                   cccTGCGGGTCAGCCTGGATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAACGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTc                                              <  1:1762231/99‑1 (MQ=255)
                                                    gcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCg                                             >  2:971467/1‑101 (MQ=255)
                                                    gcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCg                                             <  1:1049323/101‑1 (MQ=255)
                                                    gcTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGGGATGAACAATGGCACCGCGCTGGCGCTTTTGATGCCCGTCg                                             >  1:788133/1‑101 (MQ=255)
                                                     cTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGa                                            >  1:2443097/1‑101 (MQ=255)
                                                      tGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGAc                                           <  2:1298452/101‑1 (MQ=255)
                                                       gCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACa                                          >  2:721406/1‑101 (MQ=255)
                                                        ccGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACAt                                         >  1:4176424/1‑101 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                        >  1:1895316/1‑101 (MQ=255)
                                                          ggTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCg                                       >  1:3778121/1‑101 (MQ=255)
                                                          ggTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCg                                       >  1:2574281/1‑101 (MQ=255)
                                                           gTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGc                                      <  1:1169860/101‑1 (MQ=255)
                                                            tCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGcc                                     <  1:1063322/101‑1 (MQ=255)
                                                            tCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGcc                                     <  1:641632/101‑1 (MQ=255)
                                                             cAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCa                                    <  2:2846675/101‑1 (MQ=255)
                                                              aGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCAc                                   <  2:1965655/101‑1 (MQ=255)
                                                               gCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACg                                  <  1:468630/101‑1 (MQ=255)
                                                                 cTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgc                                <  1:3692956/101‑1 (MQ=255)
                                                                 cTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgc                                >  1:1823529/1‑101 (MQ=255)
                                                                 cTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgc                                >  2:2259123/1‑101 (MQ=255)
                                                                  tGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgcg                               >  1:631886/1‑101 (MQ=255)
                                                                  tGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgcg                               >  2:1077387/1‑101 (MQ=255)
                                                                     aTGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgcgcgc                            >  1:4223220/1‑101 (MQ=255)
                                                                      tgtgGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCa                           >  2:2909873/1‑101 (MQ=255)
                                                                       gtgGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCaa                          <  2:3875295/101‑1 (MQ=255)
                                                                          gcgcGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGc                       >  2:1516713/1‑101 (MQ=255)
                                                                           cgcgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGcc                      <  2:3117675/101‑1 (MQ=255)
                                                                            gcgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGccc                     <  1:3555550/101‑1 (MQ=255)
                                                                             cgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCg                    >  1:101629/1‑101 (MQ=255)
                                                                               gCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgc                  <  2:1895196/101‑1 (MQ=255)
                                                                                 tGGGGGTGGTCGTCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc                <  2:2039403/101‑1 (MQ=255)
                                                                                 tGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc                >  2:3149081/1‑101 (MQ=255)
                                                                                 tGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc                <  2:1187764/101‑1 (MQ=255)
                                                                                  gggggTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgcg               >  1:3966305/1‑101 (MQ=255)
                                                                                         gtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCgg        >  1:2032653/1‑101 (MQ=255)
                                                                                         gtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCgg        <  2:1351659/101‑1 (MQ=255)
                                                                                           cggcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGc      >  1:969609/1‑101 (MQ=255)
                                                                                            gtcggGCTGCTTTCGAGTGTGATGAACAATGTCACCGCGCGGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCt     <  1:778869/99‑1 (MQ=255)
                                                                                            ggcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCt     >  2:1733010/1‑101 (MQ=255)
                                                                                             gcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTg    >  1:3193044/1‑101 (MQ=255)
                                                                                             gcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTg    <  1:4132444/101‑1 (MQ=255)
                                                                                             gcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTg    <  1:516126/101‑1 (MQ=255)
                                                                                               gggcTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAc  >  2:2439030/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGGTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTGAC  >  NC_014034/2462352‑2462547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG