Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,481,041 T→G 100% M329M (ATG→CTG)  RCAP_rcc02306 ← LysM domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,481,0410T→G100.0% 248.5 67M329M (ATG→CTG) RCAP_rcc02306LysM domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (54/13):  ref base (0/0):  total (54/13)

GCGGCGGCGGCGGGCGGCGGCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCCCATCAAGGCGCATCGCATGCGGCCCGGGCGTCTCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCG  >  NC_014034/2480944‑2481138
                                                                                                 |                                                                                                 
gcggcggcggcgggcggcggcggcggGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCCAGGcgcagcgc                                                                                                >  1:473997/1‑101 (MQ=255)
  ggcggcggcgggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCCTCGAGCGCCTCAAGGcgcagcgcat                                                                                              >  1:3581342/1‑101 (MQ=255)
   gcggcggcgggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGGCAGGACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCCGCGCATg                                                                                             >  2:346567/1‑101 (MQ=255)
     ggcggcgggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCg                                                                                           <  1:1438196/101‑1 (MQ=255)
       cggcgggcggcggcggcgggGCGACGCCCTCGAAGCCCCGCTCGGCGCCGGCCAGCACCCGGCCCGCGGCAACCCGCGCATCCAGGAGCAGCGCCCGCGGc                                                                                         >  2:2938445/1‑101 (MQ=255)
        ggcgggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGcc                                                                                        <  2:1727400/101‑1 (MQ=255)
         gcgggcggcggcggcgggGCGACGCCCTCGAAGCCCCGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGccc                                                                                       <  2:1341331/101‑1 (MQ=255)
          cgggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCg                                                                                      >  1:3123924/1‑101 (MQ=255)
          cgggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCg                                                                                      >  2:2344341/1‑101 (MQ=255)
            ggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCggg                                                                                    >  2:2054175/1‑101 (MQ=255)
            ggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCggg                                                                                    <  2:473940/101‑1 (MQ=255)
              cggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCg                                                                                  >  2:296525/1‑101 (MQ=255)
               ggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCgc                                                                                 >  1:573700/1‑100 (MQ=255)
                 cggcggcgggGCGACGCCCTCGAAGCCCTGCCCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCCCATGCGGCCCGGGCGtcg                                                                               >  1:3781052/1‑100 (MQ=255)
                  ggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGtcgc                                                                              <  2:3347973/101‑3 (MQ=255)
                  ggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGtcgc                                                                              >  2:998277/1‑99 (MQ=255)
                    cggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGGCGCcg                                                                            >  1:736892/1‑101 (MQ=255)
                     ggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCcgc                                                                           >  2:2658831/1‑101 (MQ=255)
                     ggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGGCGCcgc                                                                           >  1:1014348/1‑101 (MQ=255)
                     ggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGGCGCcgc                                                                           >  1:1921934/1‑101 (MQ=255)
                       cgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCgg                                                                         >  2:3985832/1‑101 (MQ=255)
                         gggCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGc                                                                       <  2:3344095/101‑1 (MQ=255)
                         gggCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGc                                                                       >  2:2097356/1‑101 (MQ=255)
                         gggCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCAGCCCGGGCGTCGCCGCGGGc                                                                       >  2:884205/1‑101 (MQ=255)
                           gCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAg                                                                     >  1:1318796/1‑101 (MQ=255)
                            cGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGc                                                                    >  2:1496520/1‑101 (MQ=255)
                             gACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGcc                                                                   <  1:296570/101‑1 (MQ=255)
                             gACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGcc                                                                   >  1:1114406/1‑101 (MQ=255)
                              aCGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCg                                                                  >  1:2777545/1‑101 (MQ=255)
                               cGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGc                                                                 >  2:523491/1‑101 (MQ=255)
                                  ccTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCAGGGAGCCGCCGc                                                              >  2:934314/1‑101 (MQ=255)
                                   cTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAATGCGCAGCGCATGCGGCCCCGGCGTCGCCGCGGGCACCCGCAGCg                                                             >  2:1336993/1‑101 (MQ=255)
                                   cTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGGAGCCGCCGCg                                                             >  2:1561132/1‑101 (MQ=255)
                                   cTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCg                                                             >  2:253408/1‑101 (MQ=255)
                                     cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTg                                                           >  2:1602071/1‑101 (MQ=255)
                                     cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTg                                                           >  2:150853/1‑101 (MQ=255)
                                     cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTg                                                           >  2:1330333/1‑101 (MQ=255)
                                      gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                                          <  1:2097494/101‑1 (MQ=255)
                                       aaGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGaa                                                         >  1:248567/1‑101 (MQ=255)
                                        accccTGCTCGGCGCGGGCCCGCACCCGGCCCGCGGCATCGAACGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGaaa                                                        >  2:368127/3‑101 (MQ=255)
                                          cccTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAcc                                                      <  1:1496624/101‑1 (MQ=255)
                                            cTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgc                                                    >  1:4231376/1‑101 (MQ=255)
                                             tGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgcc                                                   >  2:2033418/1‑101 (MQ=255)
                                             tGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGGGAAAccgcc                                                   >  2:3092748/1‑101 (MQ=255)
                                              gCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCAGGGCGGCGCCGCGGGCAGGCGCAGCGGGAAAacgccg                                                  >  1:3622378/1‑101 (MQ=255)
                                              gCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGACGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgccg                                                  >  2:3657338/1‑101 (MQ=255)
                                                  ggCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTccc                                              >  1:1616951/1‑101 (MQ=255)
                                                  ggCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTccc                                              >  2:1179016/1‑101 (MQ=255)
                                                  ggCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTccc                                              >  1:1722507/1‑101 (MQ=255)
                                                    cgcgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccg                                            >  2:2382273/1‑101 (MQ=255)
                                                    cgcgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccg                                            <  1:346618/101‑1 (MQ=255)
                                                      cgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgcc                                          >  1:2677972/1‑101 (MQ=255)
                                                       gggCCAGCCCCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                                         <  1:2659037/101‑1 (MQ=255)
                                                          ccAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCcgcccgcc                                      >  1:2896450/1‑101 (MQ=255)
                                                          ccAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCcgcccgcc                                      >  2:1335362/1‑101 (MQ=255)
                                                           cAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCt                                     >  2:2148708/1‑101 (MQ=255)
                                                           cAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCt                                     <  1:2054313/101‑1 (MQ=255)
                                                             gCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGc                                   >  2:1810768/1‑101 (MQ=255)
                                                              cACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGcc                                  >  2:1194041/1‑100 (MQ=255)
                                                              cACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCa                                  >  2:2816160/1‑101 (MQ=255)
                                                                cccGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCAcc                                <  2:573636/101‑1 (MQ=255)
                                                                cccGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCAcc                                >  1:1022975/1‑101 (MQ=255)
                                                                   ggCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAc                             >  1:2160069/1‑101 (MQ=255)
                                                                   ggCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAc                             >  1:3025456/1‑101 (MQ=255)
                                                                   ggCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAc                             >  2:1098181/1‑101 (MQ=255)
                                                                     cccGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAccc                           >  1:315591/1‑101 (MQ=255)
                                                                       cgcgGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCa                         >  1:480230/1‑101 (MQ=255)
                                                                         cgGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCAcc                       <  2:1014257/101‑1 (MQ=255)
                                                                         cgGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGCCCCCGCCCGCCTGCACCAACCCCAcc                       >  2:2551397/1‑101 (MQ=255)
                                                                         cgGCAGCGAGCCCATAAGGGCCCAGCGCAGGCGGCCCGGGCGTCGCCGCGGCCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCAcc                       <  2:1921808/101‑1 (MQ=255)
                                                                          ggCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCt                      >  1:2727799/1‑101 (MQ=255)
                                                                            cATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCGGCACCAACCCCACCCCg                    >  2:2060094/1‑101 (MQ=255)
                                                                             aTCGAGCGCATCAAGGCGCAGCGCACGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGCCCCCGCCCACCTCCACCCACCCCCCCTccc                   >  1:3005187/1‑99 (MQ=255)
                                                                                gAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCAt                >  2:368086/1‑101 (MQ=255)
                                                                                gAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCAt                >  2:478402/1‑101 (MQ=255)
                                                                                    gcATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTc            >  2:2475197/2‑101 (MQ=255)
                                                                                    gcATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTc            >  1:1661576/2‑101 (MQ=255)
                                                                                     cATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCg           >  2:164454/1‑101 (MQ=255)
                                                                                     cATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCg           >  2:2884424/1‑101 (MQ=255)
                                                                                          aGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATa      >  1:2462153/1‑101 (MQ=255)
                                                                                          aGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATa      >  2:228043/1‑101 (MQ=255)
                                                                                          aGGCGCAGCGCACGGGGCCCGGGCGCCCCCGCGGGCAGCCGCAGCGTGAACCCGCCGCCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATa      <  1:934399/101‑1 (MQ=255)
                                                                                           ggCGCAGCCCTTGCGGCCCGGGCTTCCCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAg     <  1:884286/101‑1 (MQ=255)
                                                                                             cgcagcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgc   >  2:72195/1‑101 (MQ=255)
                                                                                             cgcagcgcagGCGCCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgc   <  1:2884643/101‑1 (MQ=255)
                                                                                              gcagcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgcg  >  1:300782/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
GCGGCGGCGGCGGGCGGCGGCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCCCATCAAGGCGCATCGCATGCGGCCCGGGCGTCTCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCG  >  NC_014034/2480944‑2481138

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG