Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,493,385 T→A 100% S53T (TCC→ACC)  yqgF → holliday junction resolvase YqgF

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,493,3850T→A100.0% 713.8 202S53T (TCC→ACC) yqgFholliday junction resolvase YqgF
Reads supporting (aligned to +/- strand):  new base (100/101):  ref base (0/0):  total (100/102)

Only 100 of 208 total aligned reads displayed.

CCGGGCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCTCCCTCGACCTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGCC  >  NC_014034/2493287‑2493482
                                                                                                  |                                                                                                 
ccGGGCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCAcc                                                                                                 <  1:3023936/101‑1 (MQ=255)
 cGGGCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCAccc                                                                                                >  2:4067005/1‑101 (MQ=255)
  gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               <  2:3484581/101‑1 (MQ=255)
  gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               >  1:532268/1‑101 (MQ=255)
  gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               <  1:1365324/101‑1 (MQ=255)
  gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               <  2:1036786/101‑1 (MQ=255)
  gggCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCt                                                                                               >  2:1993551/1‑101 (MQ=255)
     cTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGa                                                                                            >  2:2132522/1‑101 (MQ=255)
      tCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAc                                                                                           <  2:1445772/101‑1 (MQ=255)
      tCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAc                                                                                           >  1:790316/1‑101 (MQ=255)
       cGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAcg                                                                                          >  1:2281741/1‑100 (MQ=255)
       cGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAcg                                                                                          >  1:4188629/1‑100 (MQ=255)
        gATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGAcgt                                                                                         >  1:146784/1‑99 (MQ=255)
         aTCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTg                                                                                        >  1:3091280/1‑101 (MQ=255)
         aTCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTg                                                                                        <  2:1109239/101‑1 (MQ=255)
         aTCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTg                                                                                        >  1:1097012/1‑101 (MQ=255)
          tCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTgg                                                                                       >  1:3971157/1‑101 (MQ=255)
          tCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTgg                                                                                       <  1:626683/101‑1 (MQ=255)
           cTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGc                                                                                      >  1:2802445/1‑101 (MQ=255)
                cACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATc                                                                                 >  1:2165844/1‑101 (MQ=255)
                cACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATc                                                                                 <  1:2469966/101‑1 (MQ=255)
                 aCGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCt                                                                                <  1:3043572/101‑1 (MQ=255)
                   gAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtga                                                                              <  1:511027/101‑1 (MQ=255)
                    aaGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtgat                                                                             <  1:4067266/101‑1 (MQ=255)
                     aGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtgatg                                                                            >  1:384921/1‑101 (MQ=255)
                     aGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtgatg                                                                            >  2:2608340/1‑101 (MQ=255)
                      gACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCtgatga                                                                           <  2:3231175/101‑1 (MQ=255)
                         gATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGa                                                                        >  1:2082837/1‑101 (MQ=255)
                          aTCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGAc                                                                       <  2:685337/101‑1 (MQ=255)
                           tCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGAcc                                                                      <  1:1164772/101‑1 (MQ=255)
                           tCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGAcc                                                                      >  1:2906239/1‑101 (MQ=255)
                            cGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCg                                                                     >  2:2107463/1‑101 (MQ=255)
                            cGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGCGGCGGATCTGATGAAGACCg                                                                     >  2:3727438/1‑101 (MQ=255)
                             ggggTCGCGGTCTCGGACACGCTGCGGGGCCTCGCGACGCCCCTGCGCCCGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGt                                                                    <  1:1850264/101‑1 (MQ=255)
                             ggggTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCgg                                                                    >  2:3561795/1‑100 (MQ=255)
                               ggTCGCGGTCTCGGACGCGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTgg                                                                  <  1:2132667/101‑1 (MQ=255)
                                gTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGc                                                                 >  1:8630/1‑101 (MQ=255)
                                 tCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGcc                                                                <  1:343200/101‑1 (MQ=255)
                                 tCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGcc                                                                <  1:3506096/101‑1 (MQ=255)
                                   gcgGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGa                                                              >  1:3310054/1‑101 (MQ=255)
                                   gcgGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGa                                                              <  2:1927558/101‑1 (MQ=255)
                                    cgGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAg                                                             >  1:656751/1‑101 (MQ=255)
                                     ggTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGc                                                            >  2:33356/1‑101 (MQ=255)
                                     ggTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGc                                                            >  2:4030370/1‑101 (MQ=255)
                                     ggTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGc                                                            >  2:1240275/1‑101 (MQ=255)
                                       tctcGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCgg                                                          <  2:819511/101‑1 (MQ=255)
                                        ctcGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGc                                                         <  2:3091050/101‑1 (MQ=255)
                                         tcGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCa                                                        <  1:336814/101‑1 (MQ=255)
                                          cGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaa                                                       >  1:1765967/1‑101 (MQ=255)
                                          cGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaa                                                       <  1:663103/101‑1 (MQ=255)
                                           ggACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaaa                                                      >  1:1796087/1‑101 (MQ=255)
                                           ggACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaaa                                                      >  2:3154257/1‑101 (MQ=255)
                                           ggACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaaa                                                      >  2:3917381/1‑101 (MQ=255)
                                           ggACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCaaa                                                      <  2:2298754/101‑1 (MQ=255)
                                            gACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAAt                                                     >  1:1581202/1‑101 (MQ=255)
                                            gACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAAt                                                     >  2:1843460/1‑101 (MQ=255)
                                             acacGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATc                                                    >  2:2776567/1‑101 (MQ=255)
                                              cacGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCg                                                   >  1:993995/1‑101 (MQ=255)
                                              cacGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCg                                                   >  2:1731994/1‑101 (MQ=255)
                                                 gCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCg                                                >  2:1406008/1‑101 (MQ=255)
                                                  cTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCgg                                               >  1:3455348/1‑101 (MQ=255)
                                                   tGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCggg                                              <  2:1683047/101‑1 (MQ=255)
                                                       gggCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCg                                          >  2:491890/1‑101 (MQ=255)
                                                        ggCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGt                                         >  1:1720252/1‑101 (MQ=255)
                                                            tCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTc                                     <  2:2906020/101‑1 (MQ=255)
                                                              gcgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCgg                                   >  1:2229351/1‑101 (MQ=255)
                                                              gcgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCgg                                   <  1:2107601/101‑1 (MQ=255)
                                                               cgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATTGCCGGGTTCGTCCTCggg                                  >  1:1969772/1‑101 (MQ=255)
                                                               cgACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCggg                                  >  1:2338967/1‑101 (MQ=255)
                                                                gACGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGc                                 <  2:2210926/101‑1 (MQ=255)
                                                                  cGCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTg                               <  1:2776786/101‑1 (MQ=255)
                                                                   gCCCCTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGc                              >  1:2677122/1‑101 (MQ=255)
                                                                       cTGCGCACGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGt                          >  1:4016856/1‑101 (MQ=255)
                                                                             aCGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACAt                    >  2:1472169/1‑101 (MQ=255)
                                                                              cGATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATg                   <  2:1870270/101‑1 (MQ=255)
                                                                               gATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgg                  >  1:1027059/1‑101 (MQ=255)
                                                                               gATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgg                  <  2:1312008/101‑1 (MQ=255)
                                                                               gATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgg                  >  1:913249/1‑101 (MQ=255)
                                                                               gATCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATATGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATgg                  >  2:53443/1‑101 (MQ=255)
                                                                                aTCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGa                 <  1:2608531/101‑1 (MQ=255)
                                                                                 tCCGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGAc                >  2:3204456/1‑101 (MQ=255)
                                                                                 tCCAGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGAc                <  2:2821932/101‑1 (MQ=255)
                                                                                   cGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACgg              <  2:1492450/101‑1 (MQ=255)
                                                                                   cGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACgg              <  2:52464/101‑1 (MQ=255)
                                                                                   cGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACgg              >  2:4151204/1‑101 (MQ=255)
                                                                                   cGGCGCGAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCCCGGGCTGCCGGTGAACATGGACgg              <  1:2536984/101‑1 (MQ=255)
                                                                                      cgcgAGAAATTCACCCTCGGCGTGGCGGAACTGATGAAGACCGTGGCCCAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTc           <  1:2506075/101‑1 (MQ=255)
                                                                                      cgcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTc           <  2:3951342/101‑1 (MQ=255)
                                                                                       gcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTcc          <  1:2838454/101‑1 (MQ=255)
                                                                                       gcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTcc          <  1:3791282/101‑1 (MQ=255)
                                                                                       gcgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTcc          >  2:1787929/1‑101 (MQ=255)
                                                                                        cgAGAAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCg         <  1:427146/101‑1 (MQ=255)
                                                                                          agaAATTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAg       >  2:3015598/1‑101 (MQ=255)
                                                                                            aaaTTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAggg     >  1:2663480/1‑101 (MQ=255)
                                                                                            aaaTTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAggg     >  1:3476566/1‑101 (MQ=255)
                                                                                             aaTTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAgggg    >  1:892563/1‑101 (MQ=255)
                                                                                              aTTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGc   >  2:161280/1‑101 (MQ=255)
                                                                                              aTTCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGc   <  1:1103770/101‑1 (MQ=255)
                                                                                               ttCACCCTCGACGTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTCCCGGTGAACATGGACGGCTCCGAGGGGcc  >  2:4189393/1‑101 (MQ=255)
                                                                                               ttCACCCTCGACGGGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGcc  <  2:933986/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCGGGCTCGATCTGGGCACGAAGACGATCGGGGTCGCGGTCTCGGACACGCTGCGGGGCATCGCGACGCCCCTGCGCACGATCCGGCGCGAGAAATTCTCCCTCGACCTGGCGGATCTGATGAAGACCGTGGCCGAGCGGCAAATCGCCGGGTTCGTCCTCGGGCTGCCGGTGAACATGGACGGCTCCGAGGGGCC  >  NC_014034/2493287‑2493482

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG