Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,014 A→G 7.0% intergenic (+62/‑180) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0140A→G 7.0% 17.4 ‑1.1 ‑0.9143intergenic (+62/‑180)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (57/76):  total (63/80)
Fisher's exact test strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.28e-01

Only 100 of 155 total aligned reads displayed.

AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113
                                                                                                     |                                                                                                        
aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                                          <  2:3632310/101‑1 (MQ=21)
 gcgcCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                                         <  1:967855/101‑1 (MQ=21)
   gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                       <  2:2745107/101‑1 (MQ=21)
    ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                      <  1:93742/101‑1 (MQ=21)
     cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                     <  2:2252057/101‑1 (MQ=21)
     cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                     <  1:3543301/101‑1 (MQ=21)
        ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGGGCTGCCCCCCGTCCGCCAGCGGGCTCCCCCCGGGATATTTTCAGCAAGATGAAACCGGATGc                                                                                                  >  2:2461382/1‑101 (MQ=18)
        ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                  <  1:2661231/101‑1 (MQ=21)
          cgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                >  2:1314684/1‑101 (MQ=21)
           gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                               <  2:1517101/101‑1 (MQ=21)
            ggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                              <  2:2677322/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             >  1:2611616/1‑101 (MQ=21)
             gcgcgcGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             <  2:1834049/101‑1 (MQ=21)
             gcgcgcGGCACGACAGGCCGAGGAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                             <  2:1972186/101‑1 (MQ=18)
              ggcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                            <  2:1565830/100‑1 (MQ=18)
              cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGGCGCTGCCCCCCGCCCGCAAGCGGACTCCCCCCGGGAATTTTGCAGCAAAATGAAACCGGAGGCCTTTc                                                                                             >  1:1059554/1‑101 (MQ=18)
              cgcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                            <  1:3896555/101‑1 (MQ=21)
               gcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGACTTTCAt                                                                                           >  2:734027/1‑101 (MQ=18)
                 gcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                         >  1:3138380/1‑101 (MQ=21)
                 gcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                         <  2:1549294/101‑1 (MQ=21)
                  cggccggACAGGCCGAGTAAG.GGGGGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                        <  1:2413190/95‑1 (MQ=18)
                  cgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                        <  1:134877/101‑1 (MQ=21)
                   ggCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                       <  1:1480471/101‑1 (MQ=21)
                    gCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                      <  1:3274269/101‑1 (MQ=21)
                    gCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                      >  2:1165122/1‑101 (MQ=21)
                     cACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                     >  1:1014986/1‑101 (MQ=21)
                      aCGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                    <  2:2354520/101‑1 (MQ=21)
                          cAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                <  2:3183986/101‑1 (MQ=21)
                          cAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                >  2:3814169/1‑101 (MQ=21)
                          cAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGGCATTTGCAGAAAGATGAAAACGGATGCCTTTTATCTTGTCCGaca                                                                                >  1:833567/1‑99 (MQ=17)
                               cGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                           <  1:3496873/101‑1 (MQ=21)
                                gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                          <  1:275202/101‑1 (MQ=18)
                                gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                          >  2:3928627/1‑101 (MQ=18)
                                gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCCTGCCCGAAATATccc                                                                          >  2:4030906/1‑101 (MQ=18)
                                gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATccc                                                                          >  1:3947124/1‑101 (MQ=18)
                                 aGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                         <  1:1137456/101‑1 (MQ=18)
                                  aggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                         <  2:177793/95‑1 (MQ=18)
                                    taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                       >  1:1814213/5‑101 (MQ=255)
                                    taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCACGATGAAACCGGATGCCTTTCATATTGCCCGCAATATCCCcgg                                                                       >  2:2879137/5‑101 (MQ=18)
                                     aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                      <  1:2488527/98‑1 (MQ=255)
                                      aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                     <  2:2205881/99‑1 (MQ=255)
                                       ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                    >  1:2602730/2‑101 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCggggggg                                                                   >  2:1224466/1‑100 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                   >  1:2476508/1‑100 (MQ=255)
                                        gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                   >  1:1407280/1‑101 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcgggggcg                                                                  >  1:4000270/1‑99 (MQ=255)
                                          gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                 >  1:2113492/1‑101 (MQ=255)
                                             ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                             >  1:1980829/1‑101 (MQ=255)
                                               cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                           <  1:4123983/101‑1 (MQ=255)
                                                 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                      >  2:3680676/1‑101 (MQ=255)
                                                  tccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                     <  1:1852439/99‑2 (MQ=255)
                                                  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                     >  1:3321008/1‑100 (MQ=255)
                                                   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                    <  2:4031543/101‑3 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                  <  1:157847/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGC.TGGC..G.aagcc                                                  >  2:2595949/1‑97 (MQ=18)
                                                     ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                 >  1:3490525/1‑96 (MQ=18)
                                                       cccgtCCGCAATCTGACTCCCCCCGTGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                <  1:713144/97‑7 (MQ=18)
                                                       cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                <  1:1478641/97‑7 (MQ=255)
                                                       cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                <  1:2631089/97‑7 (MQ=255)
                                                                aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                                        <  1:1217599/101‑1 (MQ=18)
                                                                aaGCGCACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                                        <  1:2125088/101‑1 (MQ=18)
                                                                 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCag                                       <  2:1980703/101‑2 (MQ=18)
                                                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCaccgc                                    >  1:1861491/1‑97 (MQ=17)
                                                                     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc                                 >  2:3495375/1‑101 (MQ=17)
                                                                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccc                                >  2:181846/1‑101 (MQ=17)
                                                                       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccc                               <  1:2553565/101‑1 (MQ=17)
                                                                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccccc                              >  2:633975/1‑101 (MQ=17)
                                                                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.ccccc                              >  2:321368/1‑101 (MQ=18)
                                                                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                            <  2:936538/101‑3 (MQ=17)
                                                                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                            >  2:3155734/1‑99 (MQ=17)
                                                                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                            <  1:4113550/101‑3 (MQ=17)
                                                                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.acccccg                            >  2:2285455/1‑99 (MQ=17)
                                                                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc                        <  2:3008822/101‑1 (MQ=17)
                                                                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                       >  1:176553/1‑101 (MQ=2)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                       >  1:1411281/1‑101 (MQ=17)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                       <  2:1524554/101‑1 (MQ=17)
                                                                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                      >  1:286327/1‑100 (MQ=2)
                                                                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                      >  1:1383546/1‑100 (MQ=0)
                                                                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                      <  1:76569/101‑2 (MQ=0)
                                                                                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                      >  2:3035939/1‑101 (MQ=18)
                                                                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggc                     >  2:2615727/1‑101 (MQ=18)
                                                                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.CGCGCCCCCCCGCCCcggc                     >  1:1983187/1‑101 (MQ=14)
                                                                                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                   >  1:3984679/1‑101 (MQ=17)
                                                                                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                   >  2:3916301/1‑101 (MQ=18)
                                                                                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                 <  2:3744366/101‑3 (MQ=2)
                                                                                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                 <  1:582313/101‑3 (MQ=2)
                                                                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                >  1:3235319/1‑101 (MQ=17)
                                                                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCATCCC.CCCCCTGCCCCGGCGGTCCg               <  2:3138149/101‑1 (MQ=18)
                                                                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg               <  1:3695775/101‑1 (MQ=17)
                                                                                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg             <  2:1717607/101‑1 (MQ=17)
                                                                                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg            <  2:1059460/101‑1 (MQ=17)
                                                                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg         >  1:4224779/1‑101 (MQ=17)
                                                                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg         <  1:2538613/101‑1 (MQ=17)
                                                                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg         >  2:625069/1‑101 (MQ=18)
                                                                                                aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTCCGTGgcggcg       >  1:2845295/1‑101 (MQ=18)
                                                                                                aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg       <  1:3195103/101‑1 (MQ=17)
                                                                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGGGGCGcc     >  2:1265273/1‑101 (MQ=17)
                                                                                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc     >  1:335947/1‑101 (MQ=17)
                                                                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt    >  2:911065/1‑101 (MQ=17)
                                                                                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt    <  1:3680930/101‑1 (MQ=17)
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AGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTG  >  NC_014034/2493915‑2494113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG