Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,016 C→G 6.9% intergenic (+64/‑178) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0160C→G 6.9% 17.8 ‑1.1 ‑0.5144intergenic (+64/‑178)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (59/75):  total (65/79)
Fisher's exact test strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.89e-01

Only 100 of 155 total aligned reads displayed.

GCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC  >  NC_014034/2493918‑2494115
                                                                                                    |                                                                                                          
gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                           <  2:2745107/101‑1 (MQ=21)
gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                           >  2:2630897/1‑101 (MQ=21)
gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                           <  2:2226720/101‑1 (MQ=21)
 ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                          <  1:93742/101‑1 (MQ=21)
   gcggcgGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                        <  1:3278494/101‑1 (MQ=21)
    cggcggGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                       <  2:1528402/101‑1 (MQ=21)
     ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGGGCTGCCCCCCGTCCGCCAGCGGGCTCCCCCCGGGATATTTTCAGCAAGATGAAACCGGATGc                                                                                                      >  2:2461382/1‑101 (MQ=18)
     ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                      <  1:2661231/101‑1 (MQ=21)
       cgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                    >  2:1314684/1‑101 (MQ=21)
        gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                   >  2:1217505/1‑101 (MQ=21)
        gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCCGGCTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCtt                                                                                                   >  2:1852319/1‑101 (MQ=21)
         ggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                  <  2:3798128/101‑1 (MQ=21)
          gcgcgcGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  2:1834049/101‑1 (MQ=21)
          gcgcgcGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 >  1:2611616/1‑101 (MQ=21)
           ggcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                <  2:1565830/100‑1 (MQ=18)
           cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGGCGCTGCCCCCCGCCCGCAAGCGGACTCCCCCCGGGAATTTTGCAGCAAAATGAAACCGGAGGCCTTTc                                                                                                 >  1:1059554/1‑101 (MQ=18)
           cgcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                <  1:337161/101‑1 (MQ=21)
            gcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGACTTTCAt                                                                                               >  2:734027/1‑101 (MQ=18)
              gcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                             >  1:3138380/1‑101 (MQ=21)
               cggccggACAGGCCGAGTAAG.GGGGGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                            <  1:2413190/95‑1 (MQ=18)
               cgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                            <  1:134877/101‑1 (MQ=21)
                ggCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                           <  1:1480471/101‑1 (MQ=21)
                 gCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                          >  2:1165122/1‑101 (MQ=21)
                  cACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                         >  1:1014986/1‑101 (MQ=21)
                   aCGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGccc                                                                                        >  2:3040367/1‑101 (MQ=18)
                       cAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGGCATTTGCAGAAAGATGAAAACGGATGCCTTTTATCTTGTCCGaca                                                                                    >  1:833567/1‑99 (MQ=17)
                         ggCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                  <  1:3201927/101‑1 (MQ=21)
                            cGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                               <  1:746828/101‑1 (MQ=21)
                             gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              <  1:275202/101‑1 (MQ=18)
                             gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  2:3928627/1‑101 (MQ=18)
                             gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCCTGCCCGAAATATccc                                                                              >  2:4030906/1‑101 (MQ=18)
                             gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              >  1:3947124/1‑101 (MQ=18)
                              aGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  1:1137456/101‑1 (MQ=18)
                               aggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                             <  2:177793/95‑1 (MQ=18)
                                gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                            <  1:41580/96‑1 (MQ=255)
                                 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                           >  1:1814213/5‑101 (MQ=255)
                                 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCACGATGAAACCGGATGCCTTTCATATTGCCCGCAATATCCCcgg                                                                           >  2:2879137/5‑101 (MQ=18)
                                 taaggggggggCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCCGATGCCTGTCATCTTTGCCGAAACATCCCcgg                                                                           >  2:2503285/5‑101 (MQ=14)
                                 aagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                           <  1:3531791/97‑1 (MQ=255)
                                  aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                          <  1:2488527/98‑1 (MQ=255)
                                   aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         <  2:2205881/99‑1 (MQ=255)
                                   aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         >  2:1781926/3‑101 (MQ=255)
                                    ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        >  1:2602730/2‑101 (MQ=255)
                                     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                       >  1:2476508/1‑100 (MQ=255)
                                     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                       <  2:3679823/101‑1 (MQ=255)
                                     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                       >  1:1407280/1‑101 (MQ=255)
                                      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcgggggcg                                                                      >  1:4000270/1‑99 (MQ=255)
                                       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                     >  1:2113492/1‑101 (MQ=255)
                                        ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                    <  1:3964032/101‑1 (MQ=255)
                                         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  >  1:2855095/1‑101 (MQ=255)
                                          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                 >  1:1980829/1‑101 (MQ=255)
                                            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                               <  1:4123983/101‑1 (MQ=255)
                                              cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                          >  2:3680676/1‑101 (MQ=255)
                                               tccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                         <  1:1852439/99‑2 (MQ=255)
                                               tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                         >  1:3321008/1‑100 (MQ=255)
                                                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                        >  1:301015/1‑99 (MQ=255)
                                                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                        <  2:4031543/101‑3 (MQ=255)
                                                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                      <  1:157847/101‑5 (MQ=255)
                                                 cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                       <  2:1014895/101‑4 (MQ=255)
                                                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                     >  1:3490525/1‑96 (MQ=18)
                                                    cccgtCCGCAATCTGACTCCCCCCGTGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                    <  1:713144/97‑7 (MQ=18)
                                                    cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                    <  1:1478641/97‑7 (MQ=255)
                                                    cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                    <  1:2631089/97‑7 (MQ=255)
                                                     ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                   >  2:3182049/4‑94 (MQ=255)
                                                      cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                  <  1:4031167/99‑9 (MQ=255)
                                                             caGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCa                                          <  2:1690685/100‑1 (MQ=18)
                                                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCa                                          <  1:1217599/101‑1 (MQ=18)
                                                             aaGCGCACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCa                                          <  1:2125088/101‑1 (MQ=18)
                                                              aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCag                                         <  2:1980703/101‑2 (MQ=18)
                                                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCaccgc                                      >  1:1861491/1‑97 (MQ=17)
                                                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                 <  2:1471921/101‑1 (MQ=17)
                                                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                 <  1:2553565/101‑1 (MQ=17)
                                                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                 >  2:231394/1‑101 (MQ=18)
                                                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.ccccc                                >  2:633975/1‑101 (MQ=17)
                                                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.ccccc                                >  2:321368/1‑101 (MQ=18)
                                                                       cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                              >  2:3155734/1‑99 (MQ=17)
                                                                       cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                              <  2:936538/101‑3 (MQ=17)
                                                                       cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.acccccg                              >  2:2285455/1‑99 (MQ=17)
                                                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGccc                           <  1:3748899/101‑1 (MQ=17)
                                                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGcccc                          <  2:3255155/101‑1 (MQ=17)
                                                                             ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcg                         >  1:176553/1‑101 (MQ=2)
                                                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                         <  2:1524554/101‑1 (MQ=17)
                                                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                         >  1:1411281/1‑101 (MQ=17)
                                                                              gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                        >  1:1383546/1‑100 (MQ=0)
                                                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                        >  2:3035939/1‑101 (MQ=18)
                                                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                       >  2:2911175/1‑99 (MQ=2)
                                                                               atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggc                       >  2:2615727/1‑101 (MQ=18)
                                                                               atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                       >  1:1983187/1‑101 (MQ=14)
                                                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AAACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                     >  1:3675887/1‑101 (MQ=2)
                                                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                     >  2:3916301/1‑101 (MQ=18)
                                                                                   ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                   <  1:582313/101‑3 (MQ=2)
                                                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                  >  1:3235319/1‑101 (MQ=17)
                                                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                 <  1:3695775/101‑1 (MQ=17)
                                                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg           <  1:2538613/101‑1 (MQ=17)
                                                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg           >  2:625069/1‑101 (MQ=18)
                                                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGGGGCGcc       >  2:1265273/1‑101 (MQ=17)
                                                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt      >  2:911065/1‑101 (MQ=17)
                                                                                                   ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa   >  1:2841566/1‑101 (MQ=17)
                                                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc  <  1:882183/101‑1 (MQ=17)
                                                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc  >  1:3622924/1‑101 (MQ=17)
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GCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC  >  NC_014034/2493918‑2494115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG