Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 7.0% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 7.0% 18.4 ‑0.7 ‑0.3143intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (60/73):  total (66/77)
Fisher's exact test strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.73e-01

Only 100 of 154 total aligned reads displayed.

CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117
                                                                                                     |                                                                                                          
 cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                           <  1:3543301/101‑1 (MQ=21)
 cgcgGCGGGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                                                                           <  2:2252057/101‑1 (MQ=21)
   cggcggGCGCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                         <  2:1528402/101‑1 (MQ=21)
    ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGGGCTGCCCCCCGTCCGCCAGCGGGCTCCCCCCGGGATATTTTCAGCAAGATGAAACCGGATGc                                                                                                        >  2:2461382/1‑101 (MQ=18)
    ggcgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                        <  1:2661231/101‑1 (MQ=21)
      cgggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                      >  2:1314684/1‑101 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                     >  2:1217505/1‑101 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCCGGCTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCtt                                                                                                     >  2:1852319/1‑101 (MQ=21)
        ggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                    <  2:2677322/101‑1 (MQ=21)
        ggcgCGCGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                    <  2:3798128/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                   <  2:1834049/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                   >  1:2611616/1‑101 (MQ=21)
          ggcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                  <  2:1565830/100‑1 (MQ=18)
          cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGGCGCTGCCCCCCGCCCGCAAGCGGACTCCCCCCGGGAATTTTGCAGCAAAATGAAACCGGAGGCCTTTc                                                                                                   >  1:1059554/1‑101 (MQ=18)
          cgcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                  <  1:3896555/101‑1 (MQ=21)
           gcgcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGACTTTCAt                                                                                                 >  2:734027/1‑101 (MQ=18)
             gcgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                               >  1:3138380/1‑101 (MQ=21)
              cggccggACAGGCCGAGTAAG.GGGGGGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                              <  1:2413190/95‑1 (MQ=18)
              cgGCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                              <  1:134877/101‑1 (MQ=21)
               ggCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                             <  1:1480471/101‑1 (MQ=21)
                gCACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                            <  1:3274269/101‑1 (MQ=21)
                 cACGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                           >  1:1014986/1‑101 (MQ=21)
                  aCGACAGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGccc                                                                                          >  2:3040367/1‑101 (MQ=18)
                       aGGCCGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                     <  1:2461566/101‑1 (MQ=21)
                           cGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                 >  1:1615732/1‑101 (MQ=18)
                           cGAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                 <  1:746828/101‑1 (MQ=21)
                            gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                <  1:2594615/101‑1 (MQ=18)
                            gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                <  1:275202/101‑1 (MQ=18)
                            gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                >  2:3928627/1‑101 (MQ=18)
                            gAGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCCTGCCCGAAATATccc                                                                                >  2:4030906/1‑101 (MQ=18)
                             aGTAAG.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                               <  1:1137456/101‑1 (MQ=18)
                              aggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                               <  2:177793/95‑1 (MQ=18)
                               gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                              <  1:41580/96‑1 (MQ=255)
                                taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                             >  1:1814213/5‑101 (MQ=255)
                                taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCACGATGAAACCGGATGCCTTTCATATTGCCCGCAATATCCCcgg                                                                             >  2:2879137/5‑101 (MQ=18)
                                taaggggggggCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCCGATGCCTGTCATCTTTGCCGAAACATCCCcgg                                                                             >  2:2503285/5‑101 (MQ=14)
                                aagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                             <  1:3531791/97‑1 (MQ=255)
                                 aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                            <  1:2488527/98‑1 (MQ=255)
                                  aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                           <  1:2500196/99‑1 (MQ=255)
                                  aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                           >  2:1781926/3‑101 (MQ=255)
                                    gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCggggggg                                                                         >  2:1224466/1‑100 (MQ=255)
                                    gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                         <  2:3679823/101‑1 (MQ=255)
                                    gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                         >  1:1407280/1‑101 (MQ=255)
                                      gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                       >  1:2113492/1‑101 (MQ=255)
                                       ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                      <  1:3964032/101‑1 (MQ=255)
                                        gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                    >  1:2855095/1‑101 (MQ=255)
                                         ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                   >  1:1980829/1‑101 (MQ=255)
                                              tccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                           <  1:1852439/99‑2 (MQ=255)
                                              tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                           >  1:3321008/1‑100 (MQ=255)
                                               gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                          <  2:4031543/101‑3 (MQ=255)
                                               gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                          >  1:703809/1‑99 (MQ=255)
                                                cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                        <  1:157847/101‑5 (MQ=255)
                                                cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                         <  2:1014895/101‑4 (MQ=255)
                                                 ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                       >  1:3490525/1‑96 (MQ=18)
                                                   cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                      <  1:2631089/97‑7 (MQ=255)
                                                   cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                      <  1:1478641/97‑7 (MQ=255)
                                                    ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                     >  2:3182049/4‑94 (MQ=255)
                                                     cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                    <  1:4031167/99‑9 (MQ=255)
                                                      gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                   >  2:1266907/2‑92 (MQ=255)
                                                            caGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCa                                            <  2:1690685/100‑1 (MQ=18)
                                                            aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCa                                            <  1:1217599/101‑1 (MQ=18)
                                                            aaGCGCACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCa                                            <  1:2125088/101‑1 (MQ=18)
                                                             aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCag                                           <  2:1980703/101‑2 (MQ=18)
                                                                ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGCaccgc                                        >  1:1861491/1‑97 (MQ=17)
                                                                   cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                   >  2:231394/1‑101 (MQ=18)
                                                                   cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                   <  2:1471921/101‑1 (MQ=17)
                                                                   cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccc                                   <  1:2553565/101‑1 (MQ=17)
                                                                    tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.ccccc                                  >  2:321368/1‑101 (MQ=18)
                                                                    tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.ccccc                                  >  2:633975/1‑101 (MQ=17)
                                                                      cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                                >  2:3155734/1‑99 (MQ=17)
                                                                      cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                                <  2:936538/101‑3 (MQ=17)
                                                                      cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccct                                <  1:4113550/101‑3 (MQ=17)
                                                                      cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.acccccg                                >  2:2285455/1‑99 (MQ=17)
                                                                        cccGGGATAGTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCG.cccccctgc                              <  2:2113354/101‑5 (MQ=17)
                                                                          cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGccc                             <  1:3748899/101‑1 (MQ=17)
                                                                           gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGcccc                            <  2:3255155/101‑1 (MQ=17)
                                                                            ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                           <  2:1524554/101‑1 (MQ=17)
                                                                            ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                           >  1:1411281/1‑101 (MQ=17)
                                                                             gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                          >  1:1383546/1‑100 (MQ=0)
                                                                             gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                          >  1:286327/1‑100 (MQ=2)
                                                                             gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                          <  1:76569/101‑2 (MQ=0)
                                                                             gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                          >  2:3035939/1‑101 (MQ=18)
                                                                              atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                         >  2:2911175/1‑99 (MQ=2)
                                                                              atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggc                         >  2:2615727/1‑101 (MQ=18)
                                                                              atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                         >  1:1983187/1‑101 (MQ=14)
                                                                                atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AAACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                       >  1:3675887/1‑101 (MQ=2)
                                                                                atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                       >  1:3984679/1‑101 (MQ=17)
                                                                                atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                       >  2:3916301/1‑101 (MQ=18)
                                                                                  ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                     <  1:582313/101‑3 (MQ=2)
                                                                                  ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                     <  2:3744366/101‑3 (MQ=2)
                                                                                   tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                    >  1:3235319/1‑101 (MQ=17)
                                                                                       gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                <  2:1059460/101‑1 (MQ=17)
                                                                                          aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg             >  2:625069/1‑101 (MQ=18)
                                                                                            aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg           <  1:3195103/101‑1 (MQ=17)
                                                                                              gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc         >  1:335947/1‑101 (MQ=17)
                                                                                               aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt        >  2:911065/1‑101 (MQ=17)
                                                                                               aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt        <  1:3680930/101‑1 (MQ=17)
                                                                                                 aCCGGATGCCTTCCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCAGGCCGTGGGGGGGCCTTg      >  1:932663/1‑101 (MQ=9)
                                                                                                  ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa     >  1:2841566/1‑101 (MQ=17)
                                                                                                   cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  1:882183/101‑1 (MQ=17)
                                                                                                     |                                                                                                          
CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA..GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG