Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,061 G→A 7.8% intergenic (+109/‑133) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0610G→A 7.8% 18.5 0.0 ‑0.9116intergenic (+109/‑133)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/4):  ref base (56/50):  total (62/54)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.29e-01

Only 100 of 125 total aligned reads displayed.

CTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493962‑2494151
                                                                                                      |                                                                                                
cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                                >  2:3680676/1‑101 (MQ=255)
 tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGCA.TGGC..G.aa                                                                                               >  1:123812/1‑100 (MQ=255)
  gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                              >  1:301015/1‑99 (MQ=255)
  gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                                                              >  1:703809/1‑99 (MQ=255)
   cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGC.TGGC..G.aagcc                                                                                            >  2:2595949/1‑97 (MQ=18)
   cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                             <  2:1014895/101‑4 (MQ=255)
    ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                                                           >  1:3490525/1‑96 (MQ=18)
      cccgtCCGCAATCTGACTCCCCCCGTGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                          <  1:713144/97‑7 (MQ=18)
      cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                          <  1:2631089/97‑7 (MQ=255)
      cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                          <  1:1478641/97‑7 (MQ=255)
       ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                         >  2:3182049/4‑94 (MQ=255)
        cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                        <  1:4031167/99‑9 (MQ=255)
         gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                       >  2:1266907/2‑92 (MQ=255)
         gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                       <  2:4026053/100‑10 (MQ=255)
               caGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                               <  2:1690685/100‑1 (MQ=18)
               aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                               <  1:1217599/101‑1 (MQ=18)
                aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCag                                                                              <  2:1980703/101‑2 (MQ=18)
                  cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcg                                                                            >  1:262851/1‑98 (MQ=18)
                   ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                                                                           >  2:4233939/1‑97 (MQ=18)
                   ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCaccgc                                                                           >  1:1861491/1‑97 (MQ=17)
                     aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                                                                       >  2:181846/1‑101 (MQ=17)
                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                      >  2:231394/1‑101 (MQ=18)
                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                      <  1:2553565/101‑1 (MQ=17)
                      cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                      <  2:1471921/101‑1 (MQ=17)
                         cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                   >  2:3155734/1‑99 (MQ=17)
                         cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                   <  2:640139/101‑3 (MQ=17)
                         cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.acccccg                                                                   >  2:2285455/1‑99 (MQ=17)
                             cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGccc                                                                <  1:3748899/101‑1 (MQ=17)
                              gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc                                                               <  2:3255155/101‑1 (MQ=17)
                              gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc                                                               <  2:3008822/101‑1 (MQ=17)
                               ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcg                                                              >  1:176553/1‑101 (MQ=2)
                               ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                              >  1:1411281/1‑101 (MQ=17)
                               ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                              <  2:1524554/101‑1 (MQ=17)
                                gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                             <  1:76569/101‑2 (MQ=0)
                                gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                             >  1:286327/1‑100 (MQ=2)
                                gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                             >  2:3035939/1‑101 (MQ=18)
                                 atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            >  2:2911175/1‑99 (MQ=2)
                                 atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            >  1:1063102/1‑99 (MQ=2)
                                 atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            <  1:2038261/101‑3 (MQ=2)
                                 atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                            >  2:2615727/1‑101 (MQ=18)
                                 atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                                                            >  1:1983187/1‑101 (MQ=14)
                                   atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.ACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                                                          >  1:3675887/1‑101 (MQ=2)
                                   atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                          >  1:3984679/1‑101 (MQ=17)
                                   atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                          >  2:3916301/1‑101 (MQ=18)
                                     ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                                                        <  2:3744366/101‑3 (MQ=2)
                                      tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                       >  1:3235319/1‑101 (MQ=17)
                                       gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCATCCC.CCCCCTGCCCCGGCGGTCCg                                                      <  2:3138149/101‑1 (MQ=18)
                                       gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                      <  1:3695775/101‑1 (MQ=17)
                                         agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  2:1717607/101‑1 (MQ=17)
                                         agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  1:181884/101‑1 (MQ=17)
                                           caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGTggg                                                  >  1:143678/1‑100 (MQ=17)
                                             aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                <  1:2538613/101‑1 (MQ=17)
                                             aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                >  1:4224779/1‑101 (MQ=17)
                                             aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                >  2:625069/1‑101 (MQ=18)
                                               aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTCCGTGgcggcg                                              >  1:2845295/1‑101 (MQ=18)
                                               aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg                                              <  1:3195103/101‑1 (MQ=17)
                                                 gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGGGGCGcc                                            >  2:1265273/1‑101 (MQ=17)
                                                 gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            >  1:335947/1‑101 (MQ=17)
                                                  aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt                                           >  2:911065/1‑101 (MQ=17)
                                                  aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGGGGCGGCGGCt                                           >  1:2207444/1‑101 (MQ=9)
                                                    aCCGGATGCCTTCCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCAGGCCGTGGGGGGGCCTTg                                         >  1:932663/1‑101 (MQ=9)
                                                      cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  1:882183/101‑1 (MQ=17)
                                                      cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc                                       >  1:3622924/1‑101 (MQ=17)
                                                        gATGTCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCG..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                                    <  2:1353657/101‑1 (MQ=18)
                                                        gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                                    <  2:2298375/101‑1 (MQ=17)
                                                         aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.ggg                                   <  1:4122895/101‑1 (MQ=17)
                                                          tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggc                                  <  2:1784540/101‑1 (MQ=17)
                                                          tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggc                                  >  2:517161/1‑101 (MQ=17)
                                                           gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcg                                 <  2:2046365/101‑1 (MQ=17)
                                                            ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcgg                                <  1:1165216/101‑1 (MQ=17)
                                                             cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcggg                               >  2:3396601/1‑101 (MQ=18)
                                                              tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCgggg                              >  2:3468148/1‑101 (MQ=18)
                                                               ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGCGGCGGCGCCTTGAC.GGGCggggc                             >  2:3271940/1‑100 (MQ=17)
                                                                tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                            >  2:1293961/1‑101 (MQ=18)
                                                                tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                            >  2:1319747/1‑101 (MQ=18)
                                                                   tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGC.GGGCGGGGGcgcg                         >  2:1136849/1‑101 (MQ=18)
                                                                    cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCgg                        >  2:2713873/1‑101 (MQ=18)
                                                                     ttGCCAGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGGCTTGAC.GGGCGGGGGGGCggg                       >  1:43816/1‑101 (MQ=17)
                                                                        cccGAAATATCCCGGGGGGTGAA.TGAC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAt                    >  2:65134/1‑101 (MQ=18)
                                                                         ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAtt                   <  1:321413/101‑1 (MQ=18)
                                                                          cGAAATTTCCCGGGGGGGTAA.TGGC..G.AA.GCC..AGGGGGGGGCAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCACTa                  <  1:2414389/101‑1 (MQ=16)
                                                                          cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTa                  <  2:2982465/101‑1 (MQ=18)
                                                                          cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTa                  <  2:262806/101‑1 (MQ=18)
                                                                           gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTaa                 <  2:3159051/101‑1 (MQ=18)
                                                                           gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTaa                 <  1:2176365/101‑1 (MQ=18)
                                                                            aagtgtCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAAc                <  1:3190945/96‑1 (MQ=18)
                                                                              atatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCg              <  2:1554652/101‑1 (MQ=21)
                                                                               tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa             >  1:3055170/1‑101 (MQ=21)
                                                                                atCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGG.GAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGAC.GGGGGGGGGCGCGGGCAGTAACCCaa            >  1:640583/1‑101 (MQ=12)
                                                                                 tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa           >  2:2713553/1‑101 (MQ=21)
                                                                                 tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa           >  1:1570566/1‑101 (MQ=21)
                                                                                    cGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTg        >  2:1127724/1‑101 (MQ=25)
                                                                                     ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGc       >  1:1912598/1‑101 (MQ=25)
                                                                                     ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGc       <  1:4170531/101‑1 (MQ=25)
                                                                                     ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGAGGTCCGAGGGGGGGCCTTGACTGGGCGGGGG.GCGGGCGTTAACGGaagtggc       >  1:1608576/1‑96 (MQ=2)
                                                                                      gggggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  2:4162475/1‑101 (MQ=25)
                                                                                      gggggTGAA.AGGC..GAAG.CCA..GAGGGGGGCAGCCC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTTACCGAAATTGCa      <  1:2285623/101‑1 (MQ=25)
                                                                                       ggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     >  2:832539/1‑101 (MQ=25)
                                                                                        gggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc    >  1:1611537/1‑101 (MQ=25)
                                                                                          gTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg  >  1:592488/1‑101 (MQ=25)
                                                                                                      |                                                                                                
CTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493962‑2494151

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG