Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,713 G→C 100% D496E (GAC→GAG RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,7130G→C100.0% 549.0 148D496E (GAC→GAGRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (87/61):  ref base (0/0):  total (87/61)

Only 100 of 157 total aligned reads displayed.

GACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAAGTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531615‑2531810
                                                                                                  |                                                                                                  
gACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTc                                                                                                  >  2:396716/1‑101 (MQ=255)
 aCAGCCCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcg                                                                                                 <  1:716051/101‑1 (MQ=255)
    gCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggcc                                                                                              <  2:3412859/101‑1 (MQ=255)
    gCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggcc                                                                                              >  2:3783760/1‑101 (MQ=255)
      aCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcgcccac                                                                                            >  1:173795/1‑101 (MQ=255)
        cgccgcCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacgg                                                                                          >  1:2112950/1‑101 (MQ=255)
         gccgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGTCCCCGCCAGACCCGCGACGAACTcggccacggc                                                                                         >  1:3628223/1‑101 (MQ=255)
         gccgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggc                                                                                         >  1:1304637/1‑101 (MQ=255)
           cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcca                                                                                       <  2:1121090/101‑1 (MQ=255)
            gccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAg                                                                                      >  2:3512184/1‑101 (MQ=255)
            gccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAg                                                                                      >  1:2982398/1‑101 (MQ=255)
              cccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCa                                                                                    >  1:1038233/1‑101 (MQ=255)
              cccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCa                                                                                    <  2:6691/101‑1 (MQ=255)
               ccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAc                                                                                   >  1:3694378/1‑101 (MQ=255)
               ccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAc                                                                                   <  1:396767/101‑1 (MQ=255)
                cGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCGCGCAAGACCCGCGACGAACTCGGCCACGGCCAGCAcc                                                                                  >  1:3615906/1‑101 (MQ=255)
                cGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAcc                                                                                  >  2:2850736/1‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                 <  2:101253/101‑1 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                 >  1:821073/1‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                 >  2:2227975/1‑101 (MQ=255)
                  gCCGCAAGCGCCAGCACCGGCAGCCCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCa                                                                                <  1:1676705/101‑1 (MQ=255)
                  gCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCa                                                                                <  1:2064110/101‑1 (MQ=255)
                  gCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCa                                                                                <  2:1071329/101‑1 (MQ=255)
                   ccGCAAGCGCCAGCCCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAg                                                                               <  2:3734925/101‑1 (MQ=255)
                   ccGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAg                                                                               <  1:2748349/101‑1 (MQ=255)
                     gCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAgcc                                                                             >  1:1234105/1‑99 (MQ=255)
                      cAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCg                                                                            <  2:4069372/101‑1 (MQ=255)
                       aaGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCgg                                                                           >  2:658849/1‑101 (MQ=255)
                         gcgcCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTg                                                                         >  2:627115/1‑101 (MQ=255)
                           gcCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGcc                                                                       >  2:116597/1‑101 (MQ=255)
                             cAGCGCCGGCAGCCCCCGGCCGGGCGGCGCGGTGAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCa                                                                     <  1:1150251/101‑1 (MQ=255)
                               gCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGc                                                                   >  1:2283860/1‑101 (MQ=255)
                                 cccGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGccc                                                                 <  1:3922293/100‑1 (MQ=255)
                                 aCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGccc                                                                 >  1:2698727/1‑101 (MQ=255)
                                  ccGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGcccc                                                                >  2:2476100/1‑101 (MQ=255)
                                   cGGAAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCa                                                               <  2:1154496/101‑1 (MQ=255)
                                    ggCAGCCCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCAt                                                              <  1:1422363/101‑1 (MQ=255)
                                    ggCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCAt                                                              <  1:1777884/101‑1 (MQ=255)
                                     gcagcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCGGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCCCCCAGTCGGTGCCCAGCCCCATc                                                             <  2:1660071/101‑1 (MQ=255)
                                        gcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGc                                                          <  2:4130223/101‑1 (MQ=255)
                                        gcaCCCAGCCGGGCGGCGCGGGCAGAAAGGGCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGc                                                          >  2:2554321/1‑101 (MQ=255)
                                         caCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGcc                                                         >  2:2028973/1‑101 (MQ=255)
                                          aCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCa                                                        >  1:4121398/1‑101 (MQ=255)
                                           cccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCag                                                       <  1:470702/101‑1 (MQ=255)
                                           cccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCag                                                       <  2:2327875/101‑1 (MQ=255)
                                             cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                     <  2:413608/101‑1 (MQ=255)
                                             cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                     >  2:393986/1‑101 (MQ=255)
                                               gCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgcg                                                   <  2:3837193/101‑1 (MQ=255)
                                                  gggCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.gg                                               >  2:591882/1‑101 (MQ=255)
                                                  gggCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.gg                                               >  2:3654254/1‑101 (MQ=255)
                                                   ggcggcGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.ggg                                              >  2:923626/1‑101 (MQ=255)
                                                   ggcggcGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.ggg                                              >  1:3487922/1‑101 (MQ=255)
                                                   ggcggcGCGGGCAGAAAGGTCGGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.ggc                                              >  1:2402926/1‑100 (MQ=255)
                                                    gcggcgGGAGCAGAAAGGCCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGc                                             <  1:3512437/101‑1 (MQ=255)
                                                    gcggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGc                                             >  2:3140133/1‑101 (MQ=255)
                                                     cggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGcc                                            >  1:619488/1‑101 (MQ=255)
                                                     cggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGcc                                            >  2:37052/1‑101 (MQ=255)
                                                      ggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGccc                                           >  1:1881735/1‑101 (MQ=255)
                                                       gcgcGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGcccc                                          >  2:1585100/1‑101 (MQ=255)
                                                          cgGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGcc                                       >  2:129030/1‑101 (MQ=255)
                                                          cgGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGcc                                       >  1:620637/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCa                                      <  1:3569734/101‑1 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCa                                      >  2:793472/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCa                                      >  1:2014115/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCCC.CGGCCCCGCCa                                      >  1:2912174/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGCTGCCCAGCCCCATCAGCCAGAGCGC.GGGGCCCGCCa                                      >  2:3768520/1‑101 (MQ=255)
                                                              cAGAAGGGTCTCCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGAc                                   <  2:1148942/101‑1 (MQ=255)
                                                              cAGAAAGGTCGGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGAc                                   <  2:1546241/101‑1 (MQ=255)
                                                               aGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGAcc                                  >  1:2683615/1‑101 (MQ=255)
                                                               aGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGAcc                                  >  2:834263/1‑101 (MQ=255)
                                                               aGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGAcc                                  <  1:1585209/101‑1 (MQ=255)
                                                               aGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGAcc                                  >  2:3193992/1‑101 (MQ=255)
                                                               aGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCAAGTCGGTGCCACGCCCAATCA.CCCGAGCCCGGGGCCCCGCCAGCcc                                  >  2:389861/1‑101 (MQ=255)
                                                                 aaagcgcggcgcCCCCGCCAAGCCCGCCACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCa                                <  2:2927147/93‑1 (MQ=255)
                                                                 aaaGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCa                                <  2:3021117/101‑1 (MQ=255)
                                                                   aGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAgc                              >  1:689623/1‑101 (MQ=255)
                                                                    ggccggcccccccGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAgcg                             <  1:37067/93‑1 (MQ=255)
                                                                    ggTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAgcg                             >  2:2003746/1‑101 (MQ=255)
                                                                      ccTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAgcggc                           <  2:2283692/100‑1 (MQ=255)
                                                                       cTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAgcggcg                          <  2:272424/101‑1 (MQ=255)
                                                                         gcccccccGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGcc                        <  1:2543359/98‑1 (MQ=255)
                                                                          cccccccGCCAGCCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCa                       <  2:2800995/99‑1 (MQ=255)
                                                                           ccccccGCCAGCCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAg                      <  1:3928800/100‑1 (MQ=255)
                                                                            cccccGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGa                     >  2:1872685/1‑101 (MQ=255)
                                                                               ccGCCAGCCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAcc                  <  2:1555513/101‑1 (MQ=255)
                                                                               ccGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAcc                  <  2:3550116/101‑1 (MQ=255)
                                                                               ccGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAcc                  <  1:2029111/101‑1 (MQ=255)
                                                                                cGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCGCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 <  2:179884/101‑1 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                >  1:2462902/1‑101 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                >  1:3208368/1‑101 (MQ=255)
                                                                                  ccAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAAccgcc               >  2:3778564/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  2:3540735/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  2:17518/1‑101 (MQ=255)
                                                                                     gACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCgccgcc            >  1:1623722/1‑101 (MQ=255)
                                                                                     gACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCgccgcc            <  2:685737/101‑1 (MQ=255)
                                                                                      ccccGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCa           <  2:2971290/100‑1 (MQ=255)
                                                                                        ccGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGc         <  1:2372155/101‑1 (MQ=255)
                                                                                        ccGCGACAATCTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGACCAATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGc         <  2:3518315/101‑1 (MQ=255)
                                                                                              cgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCg   <  2:40359/101‑1 (MQ=255)
                                                                                               gaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCgg  >  1:3149505/1‑101 (MQ=255)
                                                                                                  |                                                                                                  
GACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAAGTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGC.GGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531615‑2531810

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG