Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,554,356 T→C 100% N96D (AAC→GAC)  mraW ← S‑adenosyl‑methyltransferase MraW

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,554,3560T→C100.0% 785.9 208N96D (AAC→GAC) mraWS‑adenosyl‑methyltransferase MraW
Reads supporting (aligned to +/- strand):  new base (92/116):  ref base (0/0):  total (92/116)

Only 100 of 216 total aligned reads displayed.

CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453
                                                                                                  |                                                                                                 
cATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCGCCCCGTCCa                                                                                                 >  2:3747597/1‑101 (MQ=255)
cATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCa                                                                                                 >  2:426000/1‑101 (MQ=255)
   ccGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGcc                                                                                              <  2:1376041/101‑1 (MQ=255)
     gCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCTCGCTCGGCCTGATCGAGCTGCATCGACGACCCGCCCAGCTCCAGCCCCACCCCGTCCACCCCt                                                                                            >  1:2956647/1‑101 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           >  1:531076/1‑101 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           <  2:3472642/101‑1 (MQ=255)
        tGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCg                                                                                         <  2:556445/101‑1 (MQ=255)
         gTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCgg                                                                                        <  2:2626396/101‑1 (MQ=255)
         gTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCgg                                                                                        >  2:3080915/1‑101 (MQ=255)
            cAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcg                                                                                     >  1:1821787/1‑101 (MQ=255)
            cAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcg                                                                                     >  2:2973821/1‑101 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    <  1:940581/101‑1 (MQ=255)
              aGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGcgcg                                                                                   >  1:4158636/1‑101 (MQ=255)
              aGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGcgcg                                                                                   >  2:626786/1‑101 (MQ=255)
               gggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGa                                                                                  >  2:2491202/1‑101 (MQ=255)
                 gCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtc                                                                                <  1:1186019/101‑1 (MQ=255)
                  cccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtct                                                                               >  2:2096866/1‑101 (MQ=255)
                  cccGGCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCCGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtct                                                                               <  1:3460146/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              <  1:3081145/101‑1 (MQ=255)
                    cGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcg                                                                             <  2:1275571/101‑1 (MQ=255)
                    cGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcg                                                                             <  2:2821201/101‑1 (MQ=255)
                    cGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGAACCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcg                                                                             <  1:1359166/101‑1 (MQ=255)
                       cgcgCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcg                                                                          >  1:2019990/1‑101 (MQ=255)
                       cgcgCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcg                                                                          >  1:424739/1‑101 (MQ=255)
                         cgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCggcggc                                                                        <  1:1923365/101‑1 (MQ=255)
                         cgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCggcggc                                                                        <  2:830333/101‑1 (MQ=255)
                          ncAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCNgcggcg                                                                       >  1:237664/2‑101 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       >  2:222443/1‑101 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       <  1:3968949/101‑1 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       <  1:3064123/101‑1 (MQ=255)
                           cAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcgc                                                                      >  1:1407202/1‑100 (MQ=255)
                           cAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGa                                                                      >  2:3537877/1‑101 (MQ=255)
                            aGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     <  2:3151828/101‑1 (MQ=255)
                              aaaGGAAAAGCCGCGCTCGGCCTGATCGAGATGCATCGACGACACGCCCAGATCCAGCACCACACCGTCCAGCCCTTCGGGCGCGATCTCGGCGGGGACgg                                                                   >  1:3572488/1‑101 (MQ=255)
                               aaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  <  2:2286406/101‑1 (MQ=255)
                                 ggAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCAt                                                                <  1:2974043/101‑1 (MQ=255)
                                  gAAAAGCCGGGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  2:1167243/101‑1 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             <  1:2901056/101‑1 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            <  2:465578/101‑1 (MQ=255)
                                      aGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAgg                                                           <  1:2845070/101‑1 (MQ=255)
                                      aGCCGCGCTCGGCCTGATCGAGCTGCATCAACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAgg                                                           >  1:3853146/1‑101 (MQ=255)
                                       gCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGt                                                          <  2:1523360/101‑1 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         <  2:2414142/101‑1 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         <  1:2252183/101‑1 (MQ=255)
                                         cgcgCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTc                                                        <  1:2203186/101‑1 (MQ=255)
                                         cgcgCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGAGGCGGCATCGAGGTTc                                                        >  2:3231976/1‑101 (MQ=255)
                                          gcgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCg                                                       <  2:3366293/101‑1 (MQ=255)
                                          gcgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCg                                                       >  1:1526162/1‑101 (MQ=255)
                                           cgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGa                                                      <  1:33499/101‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     <  1:857296/101‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     >  2:2930437/1‑101 (MQ=255)
                                              tCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaaa                                                   >  2:1915786/1‑101 (MQ=255)
                                               cGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAg                                                  <  1:470427/101‑1 (MQ=255)
                                                ggCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAgg                                                 >  2:2682629/1‑101 (MQ=255)
                                                  ccTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtg                                               <  1:1487242/101‑1 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCCCCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              <  1:798912/101‑1 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              >  1:16645/1‑101 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              >  2:3588242/1‑101 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              >  2:2134392/1‑101 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTGCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              <  1:321711/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:1448874/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  1:2065259/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:1724086/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:3975145/101‑1 (MQ=255)
                                                     gATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtc                                            <  1:2028802/101‑1 (MQ=255)
                                                     gATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtc                                            >  1:2386004/1‑101 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         <  2:4092361/101‑1 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         >  2:808082/1‑101 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         <  2:926329/101‑1 (MQ=255)
                                                         gAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGa                                        <  2:4158374/101‑1 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccag                                    <  2:3852887/101‑1 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccag                                    <  1:2892383/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  2:3234473/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   <  1:2691925/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGGTCGAAAAGGTGTTCTCGaccagc                                   >  1:3746149/1‑101 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 <  1:1106275/101‑1 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacca                               <  1:2511596/101‑1 (MQ=255)
                                                                   gacgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGGGTTCTCGaccagcaccag                              >  2:1179150/1‑101 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             <  1:2097004/101‑1 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             <  2:3923159/101‑1 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             <  2:3963525/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  1:1183136/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  1:2470297/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           >  1:1648356/1‑101 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  1:3578693/101‑1 (MQ=255)
                                                                           gCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGc                      >  1:3933722/1‑101 (MQ=255)
                                                                             ccAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCg                    >  1:1946522/1‑101 (MQ=255)
                                                                              cAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGa                   <  2:2019852/101‑1 (MQ=255)
                                                                                 aTCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                >  1:365398/1‑101 (MQ=255)
                                                                                 aTCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                >  2:3724352/1‑101 (MQ=255)
                                                                                  tCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTc               <  2:1022373/101‑1 (MQ=255)
                                                                                  tCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTc               >  2:4176258/1‑101 (MQ=255)
                                                                                    cAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgc             >  1:1895515/1‑101 (MQ=255)
                                                                                    cAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgc             <  2:1648241/101‑1 (MQ=255)
                                                                                     aGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAcccccc            >  1:3234377/1‑96 (MQ=255)
                                                                                        accaccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGCACTCGCcgcg         >  2:934579/1‑101 (MQ=255)
                                                                                        accaccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCcgcg         >  2:1850177/1‑101 (MQ=255)
                                                                                           accCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGGTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      >  2:2854656/1‑101 (MQ=255)
                                                                                               cGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGgc  <  1:2924482/101‑1 (MQ=255)
                                                                                               cGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGgc  >  1:4064267/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG