Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,609 T→G 4.9% intergenic (+81/+113) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6090T→G 4.9% 11.4 0.0 ‑0.0143intergenic (+81/+113)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (72/64):  total (76/67)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-01

Only 100 of 165 total aligned reads displayed.

GCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAG  >  NC_014034/2566512‑2566709
                                                                                                      |                                                                                                    
gCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                                                                                      <  2:1078597/101‑1 (MQ=21)
gCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                                                                                      >  1:2196690/1‑101 (MQ=21)
 cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                     <  1:2327547/101‑1 (MQ=21)
  tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                    <  1:3248456/101‑1 (MQ=21)
    gcgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                                                                                  <  1:3727302/101‑1 (MQ=21)
     cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                 <  1:2512408/101‑1 (MQ=21)
     cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                 <  1:1533020/101‑1 (MQ=21)
     cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                 <  2:3626458/101‑1 (MQ=21)
      gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                <  2:823803/101‑1 (MQ=21)
      gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                >  2:3297536/1‑101 (MQ=21)
      gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                <  2:1830164/101‑1 (MQ=21)
       cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                               <  1:509035/101‑1 (MQ=21)
        gcgcTCTGAGGCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                              <  1:2096435/101‑1 (MQ=21)
          gcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                            <  1:31132/101‑1 (MQ=21)
          gcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                            <  1:902741/101‑1 (MQ=21)
           ctctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                           <  1:2775027/101‑1 (MQ=21)
            tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                                                                                          <  1:298907/101‑1 (MQ=21)
            tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                                                                                          <  1:1173596/101‑1 (MQ=21)
             ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                         <  1:569431/101‑1 (MQ=21)
              tGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTGTTTTTTTTTCTTCTTGc                                                                                        >  2:853783/1‑101 (MQ=18)
              tGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                                        >  2:2272230/1‑101 (MQ=21)
               gagaCGAAACAGGGGGCGCTACCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGAATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                                                                                       <  1:3307486/101‑1 (MQ=21)
                agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                      <  2:236725/101‑1 (MQ=21)
                  aCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGa                                                                                    >  1:1787489/1‑101 (MQ=21)
                   cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                                   >  2:3077751/1‑101 (MQ=21)
                    gAAACGGGGGGCGCTGCTCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                  <  2:3806296/101‑1 (MQ=21)
                    gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                  <  2:1593263/101‑1 (MQ=21)
                      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGGAAATTT.GGGCCAGAAAAAGAAAGGCTTTTTCTTTCATCTTTcccccaaaa                                                                                >  1:2185736/5‑95 (MQ=2)
                      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                <  1:3581109/97‑1 (MQ=25)
                       acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                               <  2:4156408/98‑1 (MQ=25)
                        cagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg                                                                        >  1:3951563/3‑101 (MQ=0)
                         ggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                                                                             <  2:1571825/100‑1 (MQ=25)
                         ggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                                                                             <  1:949433/100‑1 (MQ=25)
                         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                       >  1:4076720/2‑101 (MQ=0)
                            gggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                    >  1:601326/1‑98 (MQ=2)
                            gggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                    >  1:1254032/1‑98 (MQ=2)
                             ggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc                                                                   <  1:1048067/101‑5 (MQ=2)
                             ggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc                                                                   <  1:2334609/101‑5 (MQ=2)
                                 cTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg                                                                     <  2:1142532/101‑1 (MQ=37)
                                        ccctcttggccTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                               <  1:1052427/91‑1 (MQ=38)
                                            cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcg                                                           >  2:3746717/7‑101 (MQ=255)
                                             ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                          <  2:2553261/96‑1 (MQ=255)
                                             ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                          <  1:3176964/96‑1 (MQ=255)
                                             ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                          <  1:3070202/96‑1 (MQ=255)
                                              tggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGACCGCTTgccgg                                                         >  2:1792567/5‑101 (MQ=255)
                                               ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                                        >  1:1210820/4‑101 (MQ=255)
                                               ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                                        >  1:721715/4‑101 (MQ=255)
                                                  cTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                     >  2:2407805/1‑101 (MQ=255)
                                                     cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                  <  2:2144179/101‑1 (MQ=255)
                                                       gCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAg                                                <  2:1958095/101‑1 (MQ=255)
                                                       gCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAg                                                <  1:3377084/101‑1 (MQ=255)
                                                          aaTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcgc                                             >  2:1742483/1‑101 (MQ=255)
                                                           aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                            >  2:722881/1‑101 (MQ=255)
                                                           aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                            >  1:2704649/1‑101 (MQ=255)
                                                             tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCAGGGGAGCCCGCTTGCGGGGGGGGGGAGCGcccc                                          >  2:1633744/1‑101 (MQ=255)
                                                              cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                         <  1:2029001/101‑1 (MQ=255)
                                                              cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                         <  1:3077981/101‑1 (MQ=255)
                                                                 ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCCGCGCCCCCGa                                       >  2:2440648/1‑101 (MQ=255)
                                                                     ccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcg                                   <  1:3962109/101‑1 (MQ=255)
                                                                     ccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcg                                   >  2:1190061/1‑101 (MQ=255)
                                                                      cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                                  >  1:946245/1‑101 (MQ=255)
                                                                       gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                 >  2:301716/1‑101 (MQ=255)
                                                                       gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                 <  1:3779274/101‑1 (MQ=255)
                                                                         gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                               >  2:579481/1‑101 (MQ=255)
                                                                         gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                               <  1:2636550/101‑1 (MQ=255)
                                                                          atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                              >  1:3851558/1‑101 (MQ=255)
                                                                            atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                            >  1:1927029/1‑101 (MQ=255)
                                                                             tttCGGACAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgc                           <  2:436788/101‑1 (MQ=255)
                                                                              ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                          <  2:3428342/101‑1 (MQ=255)
                                                                                cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                        >  1:1841706/1‑101 (MQ=255)
                                                                                cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                        >  1:3450870/1‑101 (MQ=255)
                                                                                 gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                       >  2:3547478/1‑101 (MQ=255)
                                                                                  ggCAAGTTAAAGAAGGTTTTTTCCTTTCTTCTTGCCCGAAATATCCCGGGGGATCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                      <  1:3282303/101‑1 (MQ=39)
                                                                                  ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                      >  2:2047522/1‑101 (MQ=255)
                                                                                     aaGATAAAGAAGGTCTTTTCTTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                   <  1:4021231/101‑1 (MQ=255)
                                                                                     aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                   >  1:117707/1‑101 (MQ=255)
                                                                                     aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                   >  2:2700590/1‑101 (MQ=255)
                                                                                      aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                  >  1:203708/1‑101 (MQ=255)
                                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                <  2:1457617/101‑1 (MQ=255)
                                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGAAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                >  2:1937975/1‑101 (MQ=255)
                                                                                         tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGttc               >  1:3181312/1‑100 (MQ=255)
                                                                                         tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg               >  2:527904/1‑101 (MQ=255)
                                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc              >  1:794897/1‑101 (MQ=255)
                                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc              >  2:3147613/1‑101 (MQ=255)
                                                                                           aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc             >  1:3421357/1‑101 (MQ=255)
                                                                                           aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTccc             >  2:2561971/1‑101 (MQ=255)
                                                                                           aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTccc             >  1:3903167/1‑101 (MQ=255)
                                                                                            agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc            >  1:3845393/1‑101 (MQ=255)
                                                                                             gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg           >  2:2955844/1‑101 (MQ=255)
                                                                                             gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg           <  2:1222457/101‑1 (MQ=255)
                                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTtcccgc          >  2:4124807/1‑101 (MQ=255)
                                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc          >  2:3960384/1‑101 (MQ=255)
                                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc          >  2:1710587/1‑101 (MQ=255)
                                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc          >  1:3795794/1‑101 (MQ=255)
                                                                                               agGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgcc         >  2:800901/1‑101 (MQ=255)
                                                                                                  tCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa      <  1:1717589/101‑1 (MQ=255)
                                                                                                    ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg    <  1:2272398/101‑1 (MQ=255)
                                                                                                    ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg    >  2:2194908/1‑101 (MQ=255)
                                                                                                     tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg  >  1:4193889/1‑100 (MQ=255)
                                                                                                     tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga   >  1:2588182/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAG  >  NC_014034/2566512‑2566709

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG