Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,610 T→C 4.9% intergenic (+82/+112) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6100T→C 4.9% 10.4 0.0 ‑0.1143intergenic (+82/+112)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (72/64):  total (76/67)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.71e-01

Only 100 of 166 total aligned reads displayed.

CTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGG  >  NC_014034/2566513‑2566710
                                                                                                      |                                                                                                    
cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                      <  1:2327547/101‑1 (MQ=21)
cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                      <  1:2169706/101‑1 (MQ=21)
 tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                     <  1:3248456/101‑1 (MQ=21)
 tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                     >  2:1009588/1‑101 (MQ=21)
   gcgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                                                                                   <  1:3376059/101‑1 (MQ=21)
    cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                  <  2:3626458/101‑1 (MQ=21)
    cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                  <  1:1533020/101‑1 (MQ=21)
    cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                  <  1:2512408/101‑1 (MQ=21)
     gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                 <  2:823803/101‑1 (MQ=21)
     gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                 <  2:2428260/101‑1 (MQ=21)
      cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                                <  1:2076249/101‑1 (MQ=21)
         gcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                             <  1:31132/101‑1 (MQ=21)
           tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                                                                                           <  1:298907/101‑1 (MQ=21)
           tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                                                                                           <  1:1173596/101‑1 (MQ=21)
            ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                          <  1:569431/101‑1 (MQ=21)
             tGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTGTTTTTTTTTCTTCTTGc                                                                                         >  2:853783/1‑101 (MQ=18)
              gagaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                                                                                        >  2:1826958/1‑101 (MQ=21)
               agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                       <  2:236725/101‑1 (MQ=21)
                 aCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGa                                                                                     >  1:1787489/1‑101 (MQ=21)
                  cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                                    <  1:3404108/101‑1 (MQ=21)
                   gAAACGGGGGGCGCTGCTCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                   <  2:3806296/101‑1 (MQ=21)
                   gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                   <  2:1593263/101‑1 (MQ=21)
                   gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                   <  2:2702264/101‑1 (MQ=21)
                     aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGGAAATTT.GGGCCAGAAAAAGAAAGGCTTTTTCTTTCATCTTTcccccaaaa                                                                                 >  1:2185736/5‑95 (MQ=2)
                      acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                                <  2:4156408/98‑1 (MQ=25)
                       cagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg                                                                         >  1:3951563/3‑101 (MQ=0)
                        ggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                                                                              <  1:949433/100‑1 (MQ=25)
                        agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                        >  1:4076720/2‑101 (MQ=0)
                           gggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                     >  1:601326/1‑98 (MQ=2)
                           gggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                     >  1:1254032/1‑98 (MQ=2)
                            ggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc                                                                    <  1:1048067/101‑5 (MQ=2)
                            ggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc                                                                    <  1:2334609/101‑5 (MQ=2)
                              cgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCCAAATATCCCggggg                                                                        >  2:3022218/1‑101 (MQ=25)
                               gcTGCCCCC.CTCTTGGCCTTCGGCCAATTCT.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa                                                                       <  1:2866857/101‑1 (MQ=37)
                                cTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg                                                                      <  2:1142532/101‑1 (MQ=37)
                                       ccctcttggccTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                <  1:1052427/91‑1 (MQ=38)
                                       ccctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                <  1:93659/91‑1 (MQ=255)
                                         ctcttggccTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTg                                                              <  2:2117738/93‑1 (MQ=255)
                                           cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcg                                                            >  2:3746717/7‑101 (MQ=255)
                                            ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                           <  1:3176964/96‑1 (MQ=255)
                                            ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                           <  1:3070202/96‑1 (MQ=255)
                                            ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                           <  2:2553261/96‑1 (MQ=255)
                                              ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                                         >  1:1210820/4‑101 (MQ=255)
                                              ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAAGATCCCGGGGGACCCCGCCTgggggc                                                         >  1:3885267/4‑101 (MQ=25)
                                                ccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggcag                                                       >  2:1717474/2‑99 (MQ=255)
                                                    cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                   <  2:2144179/101‑1 (MQ=255)
                                                      gCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAg                                                 <  1:3377084/101‑1 (MQ=255)
                                                        cAAGTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                               <  1:1876199/101‑1 (MQ=255)
                                                         aaTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcgc                                              >  2:1742483/1‑101 (MQ=255)
                                                          aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  1:2704649/1‑101 (MQ=255)
                                                          aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  2:722881/1‑101 (MQ=255)
                                                          aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  2:1662111/1‑101 (MQ=255)
                                                          aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  1:3091680/1‑101 (MQ=255)
                                                           ttCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccc                                            >  1:652542/1‑101 (MQ=255)
                                                             cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                          <  1:2029001/101‑1 (MQ=255)
                                                                ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                                        <  1:3964337/101‑1 (MQ=255)
                                                                 cccccGAGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGaa                                       <  2:2040485/101‑1 (MQ=255)
                                                                   cccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgc                                     <  1:1742602/101‑1 (MQ=255)
                                                                   cccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgc                                     <  1:88752/101‑1 (MQ=255)
                                                                    ccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcg                                    <  1:3962109/101‑1 (MQ=255)
                                                                      gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                  <  1:3779274/101‑1 (MQ=255)
                                                                       ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCCGCGCCCCCGAAGCGccc                                 >  2:2508330/1‑101 (MQ=255)
                                                                       ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                 >  1:3557518/1‑101 (MQ=255)
                                                                        gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                <  1:2636550/101‑1 (MQ=255)
                                                                        gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                >  2:579481/1‑101 (MQ=255)
                                                                         atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                               >  2:2199887/1‑101 (MQ=255)
                                                                         atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                               >  1:3851558/1‑101 (MQ=255)
                                                                           atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                             >  1:323970/1‑101 (MQ=255)
                                                                            tttCGGACAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgc                            <  2:436788/101‑1 (MQ=255)
                                                                             ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                           <  2:3428342/101‑1 (MQ=255)
                                                                             ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                           <  2:2617717/101‑1 (MQ=255)
                                                                               cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                         >  1:3450870/1‑101 (MQ=255)
                                                                                gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                        >  2:3547478/1‑101 (MQ=255)
                                                                                 ggCAAGTTAAAGAAGGTTTTTTCCTTTCTTCTTGCCCGAAATATCCCGGGGGATCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                       <  1:3282303/101‑1 (MQ=39)
                                                                                 ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                       >  2:185544/1‑101 (MQ=255)
                                                                                    aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                    >  1:117707/1‑101 (MQ=255)
                                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 <  2:1457617/101‑1 (MQ=255)
                                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGAAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 >  2:1937975/1‑101 (MQ=255)
                                                                                        tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                >  2:527904/1‑101 (MQ=255)
                                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  1:794897/1‑101 (MQ=255)
                                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  2:3148243/1‑101 (MQ=255)
                                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  2:3147613/1‑101 (MQ=255)
                                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  1:1983891/1‑101 (MQ=255)
                                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGAGCCCCCGAAGCGCCCGACGCGCGGCGGGGTTgc               >  1:3609110/1‑101 (MQ=255)
                                                                                           agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc             >  2:3338628/1‑101 (MQ=255)
                                                                                            gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg            <  2:1222457/101‑1 (MQ=255)
                                                                                            gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg            >  2:2955844/1‑101 (MQ=255)
                                                                                             aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTtcccgc           >  2:4124807/1‑101 (MQ=255)
                                                                                             aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc           >  2:3960384/1‑101 (MQ=255)
                                                                                             aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc           >  2:1578716/1‑101 (MQ=255)
                                                                                             aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc           >  1:427595/1‑101 (MQ=255)
                                                                                              agGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgcc          >  2:800901/1‑101 (MQ=255)
                                                                                               ggTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc         <  1:3823933/101‑1 (MQ=255)
                                                                                                   ttttCCTTTTATCTTGCTCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTTCCCGCCCAagg     >  2:2357997/1‑101 (MQ=255)
                                                                                                   ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg     <  1:2272398/101‑1 (MQ=255)
                                                                                                    tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggg    >  1:4193889/1‑100 (MQ=255)
                                                                                                    tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga    >  1:2588182/1‑101 (MQ=255)
                                                                                                    tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga    >  1:3780135/1‑101 (MQ=255)
                                                                                                     ttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag   >  2:1206568/1‑101 (MQ=255)
                                                                                                      tCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg  >  1:1703814/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGG  >  NC_014034/2566513‑2566710

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG