Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,625 C→G 4.7% intergenic (+97/+97) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6250C→G 4.7% 8.2 ‑0.8 0.0157intergenic (+97/+97)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (4/4):  ref base (100/49):  total (104/53)
Fisher's exact test strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

AGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTCAG  >  NC_014034/2566528‑2566725
                                                                                                      |                                                                                                    
   cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                                                   <  1:3404108/101‑1 (MQ=21)
    gAAACGGGGGGCGCTGCTCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                                  <  2:3806296/101‑1 (MQ=21)
     aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGcccccaat                                                                                                 >  2:4223765/6‑96 (MQ=18)
      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGGAAATTT.GGGCCAGAAAAAGAAAGGCTTTTTCTTTCATCTTTcccccaaaa                                                                                                >  1:2185736/5‑95 (MQ=2)
      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                                <  1:3581109/97‑1 (MQ=25)
        cggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc                                                                                              <  2:1120311/99‑1 (MQ=25)
        cagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg                                                                                        >  1:3951563/3‑101 (MQ=0)
         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                                       >  1:4076720/2‑101 (MQ=0)
           ggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggga                                                                                     <  1:3959563/101‑3 (MQ=2)
            gggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                                    >  1:601326/1‑98 (MQ=2)
            gggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggag                                                                                    >  1:1254032/1‑98 (MQ=2)
             ggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc                                                                                   <  1:1048067/101‑5 (MQ=2)
               cgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCCAAATATCCCggggg                                                                                       >  2:3022218/1‑101 (MQ=25)
                        ccctcttggccTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                               <  1:1052427/91‑1 (MQ=38)
                        ccctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                               <  1:93659/91‑1 (MQ=255)
                          ctcttggccTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTg                                                                             <  2:2117738/93‑1 (MQ=255)
                             ttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgg                                                                          <  1:3070202/96‑1 (MQ=255)
                              tggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGACCGCTTgccgg                                                                         >  2:1792567/5‑101 (MQ=255)
                               ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                                                        >  1:1210820/4‑101 (MQ=255)
                                gccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggag                                                                       >  2:3850891/3‑99 (MQ=255)
                                     cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                  <  2:2144179/101‑1 (MQ=255)
                                      ggCCAATTCA.CCCCCCGGGATATTTTGGGCGAGATAAAGAAGGGGTTTTTCTTTTCTCTTTCCCGAAAAATCACGGGGGGGGCCGCCTCGTGGCggggggg                                                                 >  2:756706/1‑99 (MQ=16)
                                         cAAGTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                                              <  1:1876199/101‑1 (MQ=255)
                                          aaTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcgc                                                             >  2:1742483/1‑101 (MQ=255)
                                           aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                                            >  1:2704649/1‑101 (MQ=255)
                                           aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                                            >  2:1662111/1‑101 (MQ=255)
                                            ttCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccc                                                           >  1:652542/1‑101 (MQ=255)
                                             tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCAGGGGAGCCCGCTTGCGGGGGGGGGGAGCGcccc                                                          >  2:1633744/1‑101 (MQ=255)
                                              cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                                         <  1:3077981/101‑1 (MQ=255)
                                              cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                                         <  1:2029001/101‑1 (MQ=255)
                                               a.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                                        >  1:584338/1‑101 (MQ=255)
                                                 ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCCGCGCCCCCGa                                                       >  2:2440648/1‑101 (MQ=255)
                                                 ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                                                       <  1:3964337/101‑1 (MQ=255)
                                                  cccccGAGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGaa                                                      <  2:2040485/101‑1 (MQ=255)
                                                    cccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgc                                                    <  1:1742602/101‑1 (MQ=255)
                                                     ccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcg                                                   >  2:1190061/1‑101 (MQ=255)
                                                      cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                                                  >  1:946245/1‑101 (MQ=255)
                                                      cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                                                  >  1:1676698/1‑101 (MQ=255)
                                                       gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                                 <  1:3779274/101‑1 (MQ=255)
                                                        ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCCGCGCCCCCGAAGCGccc                                                >  2:2508330/1‑101 (MQ=255)
                                                        ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                                >  1:3557518/1‑101 (MQ=255)
                                                        ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGAAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                                <  2:680417/101‑1 (MQ=255)
                                                         gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                               >  2:579481/1‑101 (MQ=255)
                                                         gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                               <  1:2636550/101‑1 (MQ=255)
                                                          atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                                              >  1:3851558/1‑101 (MQ=255)
                                                            atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                                            >  1:2516173/1‑101 (MQ=255)
                                                             tttCGGACAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgc                                           <  2:436788/101‑1 (MQ=255)
                                                              ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                                          <  2:2617717/101‑1 (MQ=255)
                                                              ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                                          <  2:3428342/101‑1 (MQ=255)
                                                                cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                                        >  1:3450870/1‑101 (MQ=255)
                                                                 gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                                       >  2:3547478/1‑101 (MQ=255)
                                                                 gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                                       >  2:848799/1‑101 (MQ=255)
                                                                  ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                                      >  2:2047522/1‑101 (MQ=255)
                                                                     aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                                   >  2:2700590/1‑101 (MQ=255)
                                                                     aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                                   >  1:117707/1‑101 (MQ=255)
                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                                <  2:1457617/101‑1 (MQ=255)
                                                                        aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGAAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                                >  2:1937975/1‑101 (MQ=255)
                                                                         tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGttc                               >  1:3181312/1‑100 (MQ=255)
                                                                         tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                               >  2:527904/1‑101 (MQ=255)
                                                                         tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                               >  1:247466/1‑101 (MQ=255)
                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              >  1:794897/1‑101 (MQ=255)
                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              >  2:3148243/1‑101 (MQ=255)
                                                                          aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                              >  1:1983891/1‑101 (MQ=255)
                                                                           aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTccc                             >  1:3903167/1‑101 (MQ=255)
                                                                             gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg                           <  2:1222457/101‑1 (MQ=255)
                                                                             gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg                           >  2:2955844/1‑101 (MQ=255)
                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTtcccgc                          >  2:4124807/1‑101 (MQ=255)
                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc                          >  2:1578716/1‑101 (MQ=255)
                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc                          >  2:1710587/1‑101 (MQ=255)
                                                                              aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc                          >  1:3795794/1‑101 (MQ=255)
                                                                                ggTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc                        <  1:3823933/101‑1 (MQ=255)
                                                                                  tCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                      <  1:1717589/101‑1 (MQ=255)
                                                                                    ttttCCTTTTATCTTGCTCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTTCCCGCCCAagg                    >  2:2357997/1‑101 (MQ=255)
                                                                                    ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                    <  1:2272398/101‑1 (MQ=255)
                                                                                    ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                    >  2:2194908/1‑101 (MQ=255)
                                                                                    ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                    <  2:1249088/101‑1 (MQ=255)
                                                                                     tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggg                   >  1:3251960/1‑100 (MQ=255)
                                                                                     tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                   >  1:3121277/1‑101 (MQ=255)
                                                                                      ttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                  >  2:1206568/1‑101 (MQ=255)
                                                                                      ttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                  >  2:3637307/1‑101 (MQ=255)
                                                                                       tCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                 >  1:338741/1‑101 (MQ=255)
                                                                                       tCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                 >  2:3367572/1‑101 (MQ=255)
                                                                                        ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg                >  1:2262076/1‑101 (MQ=255)
                                                                                        ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg                <  1:4224027/101‑1 (MQ=255)
                                                                                        ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg                >  1:3230237/1‑101 (MQ=255)
                                                                                         cTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc               <  2:2559851/101‑1 (MQ=255)
                                                                                         cTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc               >  1:2963084/1‑101 (MQ=255)
                                                                                         cTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc               <  1:3297777/101‑1 (MQ=255)
                                                                                          tttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg              >  2:1659022/1‑101 (MQ=255)
                                                                                          tttCATCTTGCCCAAAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg              <  2:3979986/101‑1 (MQ=255)
                                                                                           ttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc             <  1:2440832/101‑1 (MQ=255)
                                                                                           ttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc             >  1:4099437/1‑101 (MQ=255)
                                                                                            tCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCa            >  2:3624076/1‑101 (MQ=255)
                                                                                            tCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCa            <  2:3091450/101‑1 (MQ=255)
                                                                                            tCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCa            >  2:3088180/1‑101 (MQ=255)
                                                                                             cATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCaa           >  1:1837436/1‑101 (MQ=255)
                                                                                             cATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCaa           >  1:2520118/1‑101 (MQ=255)
                                                                                              aTCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAAc          >  2:2391960/1‑101 (MQ=255)
                                                                                               tCTTGCCCGAAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAAcc         <  1:2700796/101‑1 (MQ=255)
                                                                                                  tGCACGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGcc      >  2:3979110/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
AGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTCAG  >  NC_014034/2566528‑2566725

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG