Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 257,090 C→G 100% intergenic (+24/‑175) secA → / → RCAP_rcc00221 preprotein translocase subunit SecA/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034257,0900C→G100.0% 570.5 154intergenic (+24/‑175)secA/RCAP_rcc00221preprotein translocase subunit SecA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (70/84):  ref base (0/0):  total (70/84)

Only 100 of 177 total aligned reads displayed.

GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GCCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256992‑257187
                                                                                                    |                                                                                                   
gAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCg                                                                                                   <  2:3382568/101‑1 (MQ=255)
         cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCC.ggggg                                                                                         >  1:3104207/1‑100 (MQ=255)
            aaTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGGC.GGGGGGa.a                                                                                     >  1:4153790/1‑101 (MQ=255)
            aaTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCC.ggggggga                                                                                      >  2:2221604/1‑97 (MQ=255)
            aaTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGa.a                                                                                     >  2:1485960/1‑101 (MQ=255)
               gccccctgcccctgCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.Accc                                                                                  <  2:880850/99‑1 (MQ=255)
               gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCC.gggggggacac                                                                                   >  2:35503/1‑94 (MQ=255)
               gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.Accc                                                                                  >  1:3702996/1‑101 (MQ=255)
                aCCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGG.ACCCg                                                                                 >  1:925893/1‑101 (MQ=255)
                    ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCC.GGGGGGG.AACCGGgcc                                                                             >  1:1453317/1‑101 (MQ=255)
                     tgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGG.Acccccgccc                                                                            >  1:3288519/1‑95 (MQ=255)
                     tgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGG.AACCCGgccg                                                                            >  1:4047601/1‑101 (MQ=255)
                      gcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCC.GGGGGGG.ACCCCGgcccc                                                                           >  2:3881865/1‑99 (MQ=255)
                         cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCCGGGGGGGA.ACCCGGgcgccgg                                                                         >  2:2024434/1‑96 (MQ=255)
                         cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGCC.GGGGGGG.AGCCGGGccgccgg                                                                        >  2:2656702/1‑100 (MQ=255)
                         cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGt                                                                        <  2:1163297/101‑1 (MQ=255)
                         cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGt                                                                        <  2:2840539/101‑1 (MQ=255)
                          ctgcGGCTGGGGCGAGAAGTTCAAGCCCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGtt                                                                       <  2:1976995/101‑1 (MQ=255)
                          ctgcGGCTGGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGtt                                                                       <  2:3727026/101‑1 (MQ=255)
                          ctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGGC.GGGGGGG.AACCGGGgcgccgct                                                                       >  2:2572994/1‑99 (MQ=255)
                          ctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGtt                                                                       <  1:3471131/101‑1 (MQ=255)
                           tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGCAACAAAGGCGCTGGAATATGGGG.GGCGGTCC.GGGGGAAGACCAGCGCCGCCGt                                                                        >  2:3341835/1‑101 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTc                                                                   <  1:979716/101‑1 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTc                                                                   <  1:2755576/101‑1 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGG.ACCCCGGCCGCCGgttttt                                                                   >  2:151651/1‑100 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.ACCCCGGCCGCCGgttttt                                                                   >  2:474388/1‑100 (MQ=255)
                                 tCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCAtt                                                                <  1:365221/101‑1 (MQ=255)
                                  cGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.AACCGGGCCGCCGTTTTTCAttt                                                               >  1:2188830/1‑100 (MQ=255)
                                   gggCGAGAAGTTCAAGCCCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTAt                                                              <  1:433029/101‑1 (MQ=255)
                                   gggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGGGC.GGGGGGG.AACCGGGGCGGCGGTTTTTAtttt                                                              >  1:3354203/1‑99 (MQ=255)
                                   gggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTAt                                                              <  1:548763/101‑1 (MQ=255)
                                    ggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGG.AACCGGGCCGCCGTTTTTCATTTTc                                                             >  2:3407973/1‑101 (MQ=255)
                                    ggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGG.ACCCGGGCCGCCGGTTTTCATTTTc                                                             >  1:2603500/1‑101 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCt                                                           <  1:2300719/101‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGG.AACCCGGCCCCCGGTTTTTATTATTCt                                                           >  1:720039/1‑101 (MQ=255)
                                       gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGGAACAAAGGCGCTGAAATAT.GGCGGCGGGTTG.GGGGGGA.TCCCGGGCGCCGGTTTTTGTTTTTCCt                                                           >  2:1357209/1‑101 (MQ=255)
                                       gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTg                                                          <  1:3996012/101‑1 (MQ=255)
                                       gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.AACCCGGCCGCCGTTTTTCATTATCCtt                                                          >  2:3003568/1‑100 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGt                                                         <  1:2920627/101‑1 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGtt                                                        <  1:2982780/101‑1 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGG.AACCCGGCCGCCGTTTTTTCTTATTCTGtt                                                        >  2:140249/1‑101 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.CGGGGGG.AACCGGGCCGCCCGTTTTTATTTTTCTGtt                                                        >  2:466052/1‑101 (MQ=255)
                                          aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTg                                                       <  2:4236378/101‑1 (MQ=255)
                                           aGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.ACCCGGGCCGCCGGTTTTTATTATCCTGTTgt                                                      >  1:2726/1‑100 (MQ=255)
                                           aGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.AACCGGGCCGCCGTTTTTTATTATCCTGtttt                                                      >  2:2380998/1‑99 (MQ=255)
                                            gTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGG.ACCCGGGGCGCCGGTTTTCATTATCCTGTTGAt                                                     >  2:3374835/1‑101 (MQ=255)
                                             ttCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGG.ACCCCGGCCGCCGTTTTTCATTATCCTGTTGTTc                                                    >  2:1074524/1‑101 (MQ=255)
                                                   cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGGGGTCC.GGGGGGA.ACCCCGGCCGCCCGTTTTTATTATTCTTTTTGTCTGGata                                              >  1:3324541/1‑101 (MQ=255)
                                                   cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGgta                                              >  1:3812336/1‑101 (MQ=255)
                                                     cTGCCAGGGCCGTTTGGCTCGAAATAAAGTCGGCAGAATAT.GGC.GGTGGTCG.GGTTTGT.TCCCGGTCCGCCGTTTTTCATTATCCTGTTGATCTGGATATg                                            <  1:1044643/101‑1 (MQ=255)
                                                     cTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATg                                            <  2:3894237/101‑1 (MQ=255)
                                                      tGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGt                                           <  2:325358/101‑1 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.AACCGGGGCGCCGCTTTTCATTATTCTGTTGATCTGGATATGtt                                          >  1:2208333/1‑101 (MQ=255)
                                                         cACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.ACCCGGGGCGCCGGTTTTCATTATCCTGTTGATCTGGATATGtttt                                        >  1:1342160/1‑101 (MQ=255)
                                                          aCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.CGGGTGG.AACCGGGCCGCCGGTTTTCATTATCCTCTTGGTCTGGATATGgtttt                                       >  1:1402189/1‑101 (MQ=255)
                                                            ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGGCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGGTATGttttttt                                     >  2:2186021/1‑99 (MQ=255)
                                                              ccGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.CCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTgg                                   <  1:2656908/101‑1 (MQ=255)
                                                               cGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGa                                  <  2:2615457/101‑1 (MQ=255)
                                                               cGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGa                                  <  2:2646967/101‑1 (MQ=255)
                                                               cGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGGTTTTCATTATCCTGTTTATCTGGATATGGTTTTTTGGa                                  >  2:4090169/1‑101 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaa                                 <  2:414692/101‑1 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGaa                                 >  1:247335/1‑101 (MQ=255)
                                                                 tCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.CCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaaa                                <  2:3962333/101‑1 (MQ=255)
                                                                 tCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaaa                                <  1:2684437/101‑1 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGGTATGTTTTTTTGGAAAt                               >  1:2319084/1‑101 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAAttt                             >  1:1523447/1‑101 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTc                            <  2:3046209/101‑1 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTc                            <  2:2423430/101‑1 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTTATTATCCTGTTTATCTGGATATGTTTTTTTGGAAATTTcc                           >  1:1424092/1‑101 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAAGAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTcc                           <  1:1327871/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.CCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccg                          <  1:466109/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccg                          <  2:925808/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATAT.GGC.GGCCGCCC.TGGGGGA.ACCCCGGGCGCCGCTTTTTATTTTCCTGTTGGTCTGGATATGTTTTTGGGGAAAtttata                          >  1:2478730/1‑98 (MQ=255)
                                                                         gAAACAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggc                        <  1:3408218/101‑1 (MQ=255)
                                                                         gAAACAAAGGCGATGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggc                        <  1:1998505/101‑1 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTTATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggcc                       >  1:3968192/1‑101 (MQ=255)
                                                                             cAAAGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTTCGGCCggg                    >  1:2998831/1‑101 (MQ=255)
                                                                              aaaGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGGTTTTCATTTTCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCgggg                   >  1:3683755/1‑100 (MQ=255)
                                                                               aaGGCGCTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCg                  >  2:2932908/1‑101 (MQ=255)
                                                                                 ggCGCTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGaa                <  2:3288278/101‑1 (MQ=255)
                                                                                  gcgcTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAg               <  1:1357309/101‑1 (MQ=255)
                                                                                   cgcTGAAATAT.GGC.GGCGGTCC.GGGGGGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTTCGGCCGGGCGAAgg              >  2:1460954/1‑101 (MQ=255)
                                                                                    gcTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGt             <  1:2717062/101‑1 (MQ=255)
                                                                                     cTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGGTTTTCCTTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTTCGGCCGGGCGAAgggt            >  2:3795109/1‑99 (MQ=255)
                                                                                     cTGAAATAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGAACGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGtt            <  1:3631816/101‑1 (MQ=255)
                                                                                         aaTAT.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCg        <  1:1483456/101‑1 (MQ=255)
                                                                                          atat.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGt       >  1:1884955/1‑101 (MQ=255)
                                                                                          atat.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGt       >  2:540975/1‑101 (MQ=255)
                                                                                          atat.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGt       >  1:1507167/1‑101 (MQ=255)
                                                                                          atat.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGt       >  2:2100369/1‑101 (MQ=255)
                                                                                           tat.GGG.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGtt      <  1:1167983/101‑1 (MQ=255)
                                                                                            at.GGC.GGCGGTCC.GGGTTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGttt     <  2:3683501/101‑1 (MQ=255)
                                                                                            at.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGttt     >  2:1722621/1‑101 (MQ=255)
                                                                                             t.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTc    <  1:1790762/101‑1 (MQ=255)
                                                                                             t.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTc    >  1:1131602/1‑101 (MQ=255)
                                                                                             t.GGC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTc    >  2:1925881/1‑101 (MQ=255)
                                                                                               ggC.GGCGGTCC.GGGTTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg   <  2:4047340/101‑1 (MQ=255)
                                                                                               ggC.GGCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg   <  1:1074618/101‑1 (MQ=255)
                                                                                               ggC.GGCGGTCC.GGGGCGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGAAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg   >  1:2420484/1‑101 (MQ=255)
                                                                                                gc.ggCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCgg  <  1:994803/101‑1 (MQ=255)
                                                                                                    |                                                                                                   
GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATAT.GGC.GCCGGTCC.GGGGTGA.ACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256992‑257187

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG