Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,590,068 C→G 100% V12L (GTT→CTT)  glnE ← glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,590,0680C→G100.0% 578.5 153V12L (GTT→CTT) glnEglutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  new base (59/94):  ref base (0/0):  total (59/94)

Only 100 of 158 total aligned reads displayed.

CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165
                                                                                                  |                                                                                                 
ggAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 <  1:2992281/100‑1 (MQ=255)
cGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 <  2:1358465/101‑1 (MQ=255)
 gAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGggg                                                                                                >  1:1293672/1‑101 (MQ=255)
 gAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGggg                                                                                                >  2:3271708/1‑101 (MQ=255)
   aCAGCCCTCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCg                                                                                              <  1:39853/101‑1 (MQ=255)
   aCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCg                                                                                              <  2:313061/101‑1 (MQ=255)
    cAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGt                                                                                             >  2:2189243/1‑101 (MQ=255)
     aGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTg                                                                                            <  2:2147536/101‑1 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          <  1:847004/101‑1 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          >  1:800082/1‑101 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          <  1:456794/101‑1 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          <  1:3468624/101‑1 (MQ=255)
       cccGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCg                                                                                          <  1:2291214/101‑1 (MQ=255)
        ccgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCgg                                                                                         <  2:239534/101‑1 (MQ=255)
         cgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCggg                                                                                        <  1:2604303/101‑1 (MQ=255)
           ccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTc                                                                                      >  2:2014626/1‑101 (MQ=255)
            cgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGGCa                                                                                     >  1:4097871/1‑101 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  2:1627604/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    >  2:2359805/1‑101 (MQ=255)
               gCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGc                                                                                  <  2:832157/101‑1 (MQ=255)
                cccGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCg                                                                                 <  2:1913302/101‑1 (MQ=255)
                cccGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCg                                                                                 <  2:4086523/101‑1 (MQ=255)
                 ccGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCgg                                                                                >  2:1071547/1‑101 (MQ=255)
                 ccGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCgg                                                                                <  1:1155692/101‑1 (MQ=255)
                 ccGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCgg                                                                                >  2:960462/1‑101 (MQ=255)
                   gcgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGGGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGa                                                                              <  1:2034103/101‑1 (MQ=255)
                   gcgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGa                                                                              <  2:3772543/101‑1 (MQ=255)
                    cgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGAt                                                                             <  1:1075498/101‑1 (MQ=255)
                       tCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCa                                                                          <  2:776399/101‑1 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         >  1:4205890/1‑101 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        <  1:2427683/101‑1 (MQ=255)
                             cccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACCACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGGGCGGGGCAGGCGGGATGCAAGGCTc                                                                    <  2:347724/101‑1 (MQ=255)
                               cGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCAt                                                                  <  2:3559384/101‑1 (MQ=255)
                                   tttCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgcg                                                              >  2:1963157/1‑101 (MQ=255)
                                     tCGGGCGGAAAATCGCCATAACGGGGAACGACATCGGCGGCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGcc                                                            <  2:785966/101‑1 (MQ=255)
                                     tCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGcc                                                            <  1:1727737/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           <  1:419557/101‑1 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          <  2:3937654/101‑1 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          >  2:91989/1‑101 (MQ=255)
                                        ggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGa                                                         <  1:2181189/101‑1 (MQ=255)
                                         gCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGAc                                                        <  1:960553/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      <  1:1214672/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      >  2:1815686/1‑101 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      <  1:1962444/101‑1 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     <  1:3271949/101‑1 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     <  1:1552733/101‑1 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     <  2:2698970/101‑1 (MQ=255)
                                             aaaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgc                                                    >  2:140725/1‑101 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   <  2:1049875/101‑1 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   <  1:1026927/101‑1 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   >  1:1900778/1‑101 (MQ=255)
                                               aaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccg                                                  >  2:2776371/1‑101 (MQ=255)
                                               aaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccg                                                  >  1:2918886/1‑101 (MQ=255)
                                               aaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGAGCCAGACTAgccg                                                  >  1:2082717/1‑101 (MQ=255)
                                                  cGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCACGGCTCATGGCGCCAGACTAGccgccg                                               >  2:3019406/1‑101 (MQ=255)
                                                  cGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGccgccg                                               >  2:2764641/1‑101 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           >  1:3985220/1‑101 (MQ=255)
                                                      aTAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAg                                           <  1:2197452/101‑1 (MQ=255)
                                                        aaCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcc                                         >  2:3110297/1‑101 (MQ=255)
                                                          cGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcccc                                       <  1:3537571/101‑1 (MQ=255)
                                                          cGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcccc                                       <  2:1293576/101‑1 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     <  2:4131874/101‑1 (MQ=255)
                                                              cAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGt                                   <  2:295372/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  2:3347042/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  2:319287/101‑1 (MQ=255)
                                                                aCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCa                                 <  1:1423953/101‑1 (MQ=255)
                                                                aCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCa                                 >  1:1514532/1‑101 (MQ=255)
                                                                aCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCa                                 <  1:2359981/101‑1 (MQ=255)
                                                                   aCATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAgg                              <  2:2687134/101‑1 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAGGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            <  2:134747/101‑1 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            >  1:3032071/1‑101 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            >  1:3308047/1‑101 (MQ=255)
                                                                       cGGCGCCACGGGCCGGATCAAGGGGAAGGTGGGGGCGGGTCAGGGGGGATGCAAGGCCCATGGCGCCAAACTAACCGCCGCAAGCCCCcgggcccagcgcc                          >  2:3523898/1‑91 (MQ=255)
                                                                       cGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcgcg                          >  1:547330/1‑101 (MQ=255)
                                                                          cgcCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCaa                       >  1:4095586/1‑101 (MQ=255)
                                                                           gcCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCaaa                      >  1:1739041/1‑101 (MQ=255)
                                                                           gcCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCaaa                      >  2:3682062/1‑101 (MQ=255)
                                                                            ccAGGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAAt                     <  1:2866947/101‑1 (MQ=255)
                                                                            ccACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTGGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAAt                     >  1:3603758/1‑101 (MQ=255)
                                                                             cACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATg                    >  2:2251564/1‑101 (MQ=255)
                                                                              aCGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgc                   <  1:3117474/101‑1 (MQ=255)
                                                                              aCGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCAGGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgc                   <  2:2066451/101‑1 (MQ=255)
                                                                               cGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcc                  <  1:2776590/101‑1 (MQ=255)
                                                                               cGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcc                  >  2:1756840/1‑101 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGACACGGGTCAAGGCGCGCAAATgcccc                >  2:2643229/1‑101 (MQ=255)
                                                                                   ccGAATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgc              <  2:260370/101‑1 (MQ=255)
                                                                                    cGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcc             >  2:4078160/1‑101 (MQ=255)
                                                                                     ggATCAGGGGGAAGGGGCGTGCGGGTCGGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgccc            <  1:2014764/101‑1 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          >  2:3924446/1‑101 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          <  1:1040803/101‑1 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCCCAAATGCCCCGCCCCt          <  1:1812291/101‑1 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCTGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       <  1:3750964/101‑1 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccggggcttgg       >  1:1766131/1‑93 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       >  2:3675770/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       <  1:3099317/101‑1 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       <  1:625791/101‑1 (MQ=255)
                                                                                           aGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCa      <  1:1757772/101‑1 (MQ=255)
                                                                                            ggggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccggggcttgcgg     <  2:1766012/101‑11 (MQ=255)
                                                                                            ggggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCAt     >  2:589654/1‑101 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  >  2:511419/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG