Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,595,353 G→C 100% V205V (GTG→GTC bglX → periplasmic beta‑glucosidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,595,3530G→C100.0% 650.4 183V205V (GTG→GTCbglXperiplasmic beta‑glucosidase
Reads supporting (aligned to +/- strand):  new base (90/92):  ref base (0/0):  total (90/93)

Only 100 of 197 total aligned reads displayed.

GCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCA  >  NC_014034/2595255‑2595450
                                                                                                  |                                                                                                 
gCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGa                                                                                                 <  2:2400918/101‑1 (MQ=255)
gCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGa                                                                                                 <  1:1010725/101‑1 (MQ=255)
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                <  1:1976353/101‑1 (MQ=255)
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGACCTCTCCCTCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                >  1:3613436/1‑101 (MQ=255)
   gATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGc                                                                                              <  2:665656/101‑1 (MQ=255)
   gATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGc                                                                                              >  2:1956861/1‑101 (MQ=255)
   gATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGc                                                                                              <  1:2452689/101‑1 (MQ=255)
      ggCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAgggggg                                                                                           >  2:3419102/1‑97 (MQ=255)
      ggCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAg                                                                                           >  1:1026643/1‑101 (MQ=255)
        cGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcc                                                                                         >  1:1127071/1‑101 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGCGccc                                                                                        >  2:1735113/1‑101 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGccc                                                                                        >  1:1173299/1‑101 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGccc                                                                                        <  2:2131497/101‑1 (MQ=255)
         gACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGccc                                                                                        <  1:2631616/101‑1 (MQ=255)
           cATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcg                                                                                      <  2:3724336/101‑1 (MQ=255)
            aTGGCCCGGCCCGACCGGATGATGGCGACCCGGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcgc                                                                                     >  1:3751866/1‑101 (MQ=255)
              ggcccggcccGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGa                                                                                   <  2:932050/101‑1 (MQ=255)
               gcccggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGAc                                                                                  >  1:2202229/1‑101 (MQ=255)
               gcccggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGAc                                                                                  >  1:295389/1‑101 (MQ=255)
               gcccggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGAc                                                                                  <  2:2230111/101‑1 (MQ=255)
                  cggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCt                                                                               <  2:617390/101‑1 (MQ=255)
                  cggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCt                                                                               >  1:3829756/1‑101 (MQ=255)
                    gcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGa                                                                             >  1:4155077/1‑101 (MQ=255)
                    gcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGa                                                                             <  1:3167377/101‑1 (MQ=255)
                     cccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGGCGCTGAt                                                                            <  2:862825/101‑1 (MQ=255)
                     cccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGAt                                                                            <  2:535418/101‑1 (MQ=255)
                      ccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATg                                                                           <  2:1540181/101‑1 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                         >  1:2688696/1‑101 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                         >  1:675190/1‑101 (MQ=255)
                         aCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCgg                                                                        >  2:1698913/1‑101 (MQ=255)
                         aCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCgg                                                                        <  1:514258/101‑1 (MQ=255)
                         aCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCgg                                                                        <  1:3442237/101‑1 (MQ=255)
                               tgatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATg                                                                  <  2:1126977/101‑1 (MQ=255)
                                gatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATgg                                                                 >  2:867529/1‑101 (MQ=255)
                                gatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATgg                                                                 >  2:3118488/1‑101 (MQ=255)
                                gatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATgg                                                                 >  1:289256/1‑101 (MQ=255)
                                 atgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGa                                                                >  2:2372884/1‑101 (MQ=255)
                                    gggACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGcc                                                             <  1:1571141/99‑1 (MQ=255)
                                    gCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGcc                                                             <  2:687926/101‑1 (MQ=255)
                                     cGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGccc                                                            >  2:4146462/1‑101 (MQ=255)
                                     cGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGccc                                                            >  2:2012923/1‑101 (MQ=255)
                                      gACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCt                                                           >  2:3623427/1‑101 (MQ=255)
                                        cccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTc                                                         >  1:3214257/1‑101 (MQ=255)
                                         ccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCa                                                        <  2:2086669/101‑1 (MQ=255)
                                         ccTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGATGATGCGGATCATGGAGCCCTTCa                                                        <  1:3558741/101‑1 (MQ=255)
                                          cTGAATCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaa                                                       <  1:3419347/101‑1 (MQ=255)
                                          cTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaa                                                       <  2:3299647/101‑1 (MQ=255)
                                           tGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaaa                                                      >  2:3830117/1‑101 (MQ=255)
                                           tGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaaa                                                      >  2:2485507/1‑101 (MQ=255)
                                           tGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaaa                                                      <  1:1087863/101‑1 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     <  2:1835264/101‑1 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     >  1:1630038/1‑101 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     >  1:191824/1‑101 (MQ=255)
                                            gAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAAt                                                     >  1:411437/1‑101 (MQ=255)
                                             aaGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATc                                                    >  2:3070815/1‑101 (MQ=255)
                                              aGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCg                                                   <  2:365774/101‑1 (MQ=255)
                                                    tCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTg                                             <  1:660328/101‑1 (MQ=255)
                                                           cTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGcggcgg                                      >  1:1381943/1‑101 (MQ=255)
                                                            tACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCggg                                     >  1:2177767/1‑101 (MQ=255)
                                                              cGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCggggg                                   >  1:2215599/1‑101 (MQ=255)
                                                               ggCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGc                                  >  2:442915/1‑101 (MQ=255)
                                                               ggCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGc                                  >  1:3014132/1‑101 (MQ=255)
                                                               ggCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGc                                  <  2:2421197/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                 <  2:577104/101‑1 (MQ=255)
                                                                 ccTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcg                                <  2:2911341/101‑1 (MQ=255)
                                                                 ccTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcg                                <  1:3789922/101‑1 (MQ=255)
                                                                   tGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggt                              <  1:2070859/101‑1 (MQ=255)
                                                                     tCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgc                            >  1:2090753/1‑101 (MQ=255)
                                                                      ccATCGCCGGGCGCGATTATGCGGCGGTCGGGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcg                           <  1:1735232/101‑1 (MQ=255)
                                                                       cATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcgg                          <  2:1062281/101‑1 (MQ=255)
                                                                       cATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggggcgg                          >  1:1301855/1‑101 (MQ=255)
                                                                        aTCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggggcggg                         >  1:2265050/1‑100 (MQ=255)
                                                                         tCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcggtg                        >  1:4176118/1‑101 (MQ=255)
                                                                          cGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGa                       >  2:3781938/1‑101 (MQ=255)
                                                                          cGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGGGa                       >  1:2515239/1‑101 (MQ=255)
                                                                           gCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAt                      <  2:289211/101‑1 (MQ=255)
                                                                             cGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtgg                    >  1:3408590/1‑101 (MQ=255)
                                                                             cGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtgg                    <  2:295984/101‑1 (MQ=255)
                                                                             cGGGCGCGATTATGCCGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtgg                    <  2:1357536/101‑1 (MQ=255)
                                                                                 cgcgATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGc                >  1:4027236/1‑101 (MQ=255)
                                                                                 cgcgATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGGGCGGTGATGGTGGc                >  2:2306188/1‑101 (MQ=255)
                                                                                  gcgATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGcc               <  2:1629929/101‑1 (MQ=255)
                                                                                   cgATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCt              <  1:957407/101‑1 (MQ=255)
                                                                                     aTTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCttt            <  1:3071040/101‑1 (MQ=255)
                                                                                     aTTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGGGCGGGGATGGGGGCCttt            >  1:1874827/1‑101 (MQ=255)
                                                                                      ttATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCtttt           <  2:3214018/101‑1 (MQ=255)
                                                                                      ttATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCtttt           <  2:3448125/101‑1 (MQ=255)
                                                                                      ttATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGGGATGGGGGCCtttt           >  1:3145620/1‑101 (MQ=255)
                                                                                        aTGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTcc         <  2:2588882/101‑1 (MQ=255)
                                                                                         tGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcg        <  1:3118719/101‑1 (MQ=255)
                                                                                         tGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcg        <  1:2373060/101‑1 (MQ=255)
                                                                                         tGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcg        <  1:2998640/101‑1 (MQ=255)
                                                                                          gcggcgGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcgc       <  2:1883828/101‑1 (MQ=255)
                                                                                          gcggcgGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcgc       <  2:236036/101‑1 (MQ=255)
                                                                                           cggcggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcgcg      >  2:2728817/1‑101 (MQ=255)
                                                                                            ggcggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGGGATGGTGGCCTTTTCCGCGa     >  1:787506/1‑101 (MQ=255)
                                                                                              cggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGGGCGGTGATGGGGGCCTTTTCCGCGATc   >  1:3911890/1‑101 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa  <  1:867610/101‑1 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa  <  2:3751609/101‑1 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa  <  2:660887/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCA  >  NC_014034/2595255‑2595450

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG