Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	57.4	16	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (2/14):  ref base (0/0):  total (2/14)

GGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGA  >  NC_014034/2602956‑2603143                                                                                               |                                                                                              ggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGcc                                                                                         <  1:3305328/101‑1 (MQ=255)      ggCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCgg                                                                                    <  1:3880693/101‑1 (MQ=255)         tGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccc                                                                                 <  1:1807322/101‑1 (MQ=255)         cgccgccGCGGGCAGCGAGCGGATCTACATCACCTCGCTGGCGGGGGCCGCGATCTACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccc                                                                                 <  2:1042348/100‑1 (MQ=255)            cgccgcGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCCCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCg                                                                              <  1:338660/101‑1 (MQ=255)               cgcgGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCa                                                                           <  1:484877/101‑1 (MQ=255)                  gggCAGCGAGGGCATCACCACCCCCTCGCGGGGGGGGGCCGCGCCCCCCCGCGCCGCCCCCCCCCGCCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACcg                                                                        <  2:2703976/101‑1 (MQ=255)                   ggaagcgagcgAATCTACACCCCCCCGCTGGCGGGGGCCGCGATCGACCGCACCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACcgc                                                                       <  2:1820596/98‑1 (MQ=255)                   ggCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACcgc                                                                       <  1:1169643/101‑1 (MQ=255)                   ggCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGCCCCCGATCGACCGCGCCGCCGCCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACcgc                                                                       <  2:3811440/101‑1 (MQ=255)                          agcgGATCGACATCACCTCGCGGGCGGGGGGCGCGGTCGACCCGGCCGCCGCCCCCCCCCCCCCCCCCCCGCCGCCCCCGGCCCCCCCCCCCCCGcccccc                                                                >  1:2409312/1‑99 (MQ=255)                              gATCGACATCACCTCGCTGGCGGGGGGCGCGCGGCAACCCGCCGCCCGCACCCCCCCCCCCCCCCCGGCGCCCGCGGCGCCCCCCCCCGCGcccccgcccc                                                            >  2:1753814/1‑95 (MQ=255)                                  gACACCCCCTCGCTGGCGGGGGCCGCGATCGCCCGCCCCCCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCcgccgc                                                        <  1:1753933/101‑1 (MQ=255)                                       ccccTCGCTGGGGGGGGCCGCGATCGACCGCGCCGCCTTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCaa                                                   <  2:3958315/99‑1 (MQ=255)                                           ccgggggCGGGGGCCGCGACCCCCCGCCCCGCCGCCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGc                                               <  2:2409136/96‑1 (MQ=255)                                                                 aCCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGCGGCGAAACCGgcc                         >  2:2165507/1‑101 (MQ=255)                                                                    gcgcCGCCGTCCCCCGCCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgccgcc                      <  2:1421418/101‑1 (MQ=255)                                                                                        cccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGTGAAACCGGCCGCCCCCGCCCAGACCGGCgccga  >  1:3663043/1‑101 (MQ=255)                                                                                               |                                                                                              GGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGA  >  NC_014034/2602956‑2603143 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 39 ≤  ATCG