Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,184 G→A 15.0% intergenic (‑160/+116) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1840G→A15.0% 45.9 ‑4.3 ‑0.0133intergenic (‑160/+116)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/6):  ref base (44/69):  total (58/75)
Fisher's exact test strand distribution p-value = 1.37e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.58e-01

Only 100 of 144 total aligned reads displayed.

TGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTC  >  NC_014034/2640088‑2640285
                                                                                                     |                                                                                                     
tGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTAt                                                                                                    <  2:1988366/101‑1 (MQ=255)
 tcGACGAGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CCCCCCCCGAGGTAtt                                                                                                   <  2:585320/100‑1 (MQ=25)
  cGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTAttt                                                                                                  <  2:2418875/101‑1 (MQ=255)
    aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTgg                                                                                                <  2:2249054/101‑1 (MQ=255)
     cgcgGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGa                                                                                               <  1:3522607/101‑1 (MQ=255)
      gcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGag                                                                                              <  1:1966406/101‑2 (MQ=255)
       cggcggCAGGCGTGTCAACCGGGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGagc                                                                                             <  1:758480/101‑3 (MQ=25)
        ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                            <  2:2035462/101‑1 (MQ=37)
        ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGAAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                            <  1:3662671/101‑1 (MQ=25)
           ggcAGGCGTGTCAACCGTGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGa                                                                                         <  2:694869/101‑1 (MQ=25)
            gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCCGCCCCCCCC.CTTC..CTTCCTGCCAATC.CTCCCCCCCCGCGATTTGGAGCaaaaa                                                                                        >  1:1838174/1‑98 (MQ=255)
             cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATg                                                                                       <  2:2116866/101‑1 (MQ=37)
              aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAATATGa                                                                                      <  2:4096019/101‑1 (MQ=25)
               ggggttccacccGTGGCACTTTTGCCAAGGC.AAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                    <  2:3804816/91‑1 (MQ=17)
               ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                     <  1:1203772/101‑1 (MQ=25)
                gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                    <  2:3662876/101‑1 (MQ=25)
                    gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                <  2:4056931/101‑5 (MQ=21)
                       aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                            <  2:2487968/101‑1 (MQ=21)
                       aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                            <  1:3137833/101‑1 (MQ=25)
                         ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CTGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                          <  2:3662767/101‑1 (MQ=25)
                         ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                          <  1:1451997/101‑1 (MQ=25)
                              gcgcTTCTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                     <  1:685582/101‑1 (MQ=21)
                                 cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                  <  1:3089224/101‑1 (MQ=21)
                                  ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                 <  2:3042280/101‑1 (MQ=25)
                                     tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                              <  1:3837973/101‑1 (MQ=21)
                                      gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                             <  1:251102/101‑1 (MQ=25)
                                       cgcAGGCAAAAGGGGGGGGCGGCCCCCCCCTTTGGC.CTT.CGGCCAATTACCCCCCCCGGGGT.TTTGG.GGAAGAAGAAAAAACAAGGGGTT.ATCTTTCCTCa                                                            >  2:1067304/1‑101 (MQ=255)
                                       cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                            <  1:925952/101‑1 (MQ=25)
                                         gccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                         >  1:1398393/7‑101 (MQ=14)
                                         cAGGCAAAAGGGGGGGGCCGCCCCCCCC.TTTGCCCTT.GGGCCAATT.ACCCCCCCG.GGGATTTTGGGGAAAATGAAAA.ACCATGGGTTTATCTTTCCTCaaa                                                          >  2:2451290/1‑101 (MQ=255)
                                         cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                          <  2:1433926/101‑1 (MQ=25)
                                          ccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                        >  2:723395/6‑101 (MQ=9)
                                           ggCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                        <  1:210318/101‑1 (MQ=21)
                                           cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                       <  1:536724/97‑1 (MQ=17)
                                           cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                       >  2:1809181/5‑101 (MQ=9)
                                            gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                       <  2:1115952/101‑1 (MQ=25)
                                            gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                       <  2:3949620/101‑1 (MQ=25)
                                              gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                    >  1:2592976/2‑101 (MQ=17)
                                               aaaGGGGGGAGCTGCCCCCCTC.TTGGC.CTT.TGGCCAATT.CACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATacc                                                    >  2:551273/1‑101 (MQ=18)
                                               aaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                   >  1:131524/1‑101 (MQ=17)
                                                  ggggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                 <  2:211406/101‑1 (MQ=25)
                                                  ggggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                 >  1:1356940/1‑101 (MQ=21)
                                                  ggggggCCCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                 <  2:2338473/101‑1 (MQ=25)
                                                  agggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                >  1:529732/2‑101 (MQ=18)
                                                   gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                               >  2:1643891/1‑101 (MQ=18)
                                                   gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                <  2:1443836/101‑1 (MQ=25)
                                                    ggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                              >  2:3483856/1‑100 (MQ=18)
                                                    ggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                              >  2:2878552/1‑101 (MQ=18)
                                                     gggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtg                                             >  1:4120356/1‑99 (MQ=18)
                                                     gggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                              <  1:3920565/101‑1 (MQ=25)
                                                       gcgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                           <  2:3270389/101‑5 (MQ=21)
                                                       gcgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                            <  2:3909766/101‑1 (MQ=25)
                                                        cgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                           <  2:3468420/101‑1 (MQ=25)
                                                         gcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                         >  2:3670734/1‑95 (MQ=21)
                                                          cTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGAAATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                        >  1:960724/1‑94 (MQ=25)
                                                              cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                     >  2:3092790/1‑101 (MQ=37)
                                                              cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                     <  1:14955/101‑1 (MQ=25)
                                                               ccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                    <  1:3978114/101‑1 (MQ=37)
                                                                tcttc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                   <  1:337064/98‑1 (MQ=25)
                                                                cccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                   >  1:1208728/1‑101 (MQ=37)
                                                                cccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                   >  1:1352283/1‑101 (MQ=37)
                                                                  ctc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                 >  1:336164/1‑101 (MQ=37)
                                                                       ttggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                              <  2:1208634/96‑1 (MQ=37)
                                                                       ttggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                              >  1:144686/6‑101 (MQ=37)
                                                                        tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                             >  1:3496281/5‑101 (MQ=37)
                                                                        tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACagg                             >  2:3866125/5‑101 (MQ=25)
                                                                        tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                             >  1:4197583/5‑101 (MQ=37)
                                                                         ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                            <  1:1280080/98‑1 (MQ=37)
                                                                         ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                            >  1:2898838/4‑101 (MQ=37)
                                                                           ccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                          >  2:468205/2‑101 (MQ=37)
                                                                            cTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                         >  1:824355/1‑101 (MQ=37)
                                                                             tt.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                        >  2:4017796/1‑101 (MQ=37)
                                                                             ctgcGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                         <  1:886318/98‑1 (MQ=37)
                                                                                cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                      >  1:3003586/1‑101 (MQ=255)
                                                                                 ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                     <  1:3158304/101‑1 (MQ=255)
                                                                                  gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                    >  1:1952819/1‑101 (MQ=37)
                                                                                   ccAATT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                   <  2:3002284/101‑1 (MQ=255)
                                                                                    cAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                  >  1:3660143/1‑101 (MQ=37)
                                                                                    cAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                  >  2:1709144/1‑101 (MQ=255)
                                                                                     aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                 >  2:3597345/1‑101 (MQ=37)
                                                                                      aTT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                <  2:463192/101‑1 (MQ=25)
                                                                                      aTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                >  1:2473321/1‑101 (MQ=37)
                                                                                         tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt              <  2:3330312/98‑1 (MQ=255)
                                                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc             >  2:1520481/3‑101 (MQ=255)
                                                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc             >  2:4042349/3‑101 (MQ=255)
                                                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc             >  1:1174196/3‑101 (MQ=37)
                                                                                           accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc            >  1:4120075/2‑101 (MQ=255)
                                                                                            ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc           >  1:2928867/1‑101 (MQ=255)
                                                                                             cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg          <  1:3620756/101‑1 (MQ=255)
                                                                                              ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg         <  1:1709259/101‑1 (MQ=255)
                                                                                              ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg         <  1:3093020/101‑1 (MQ=255)
                                                                                               cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg        >  2:2605875/1‑101 (MQ=255)
                                                                                               cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg        <  2:225005/101‑1 (MQ=255)
                                                                                                 cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc      <  1:3948438/101‑1 (MQ=255)
                                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt     >  2:2863901/1‑101 (MQ=255)
                                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt     >  1:2761305/1‑101 (MQ=255)
                                                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt     >  2:1804722/1‑101 (MQ=255)
                                                                                                  gAGGTAGTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt     <  1:2254934/101‑1 (MQ=255)
                                                                                                   aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt    <  1:2793907/101‑1 (MQ=255)
                                                                                                     gTATTTGGAGCAAGATG.AAAGACCATGCGTTCATCTGGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc  <  1:1785083/101‑1 (MQ=25)
                                                                                                     |                                                                                                     
TGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTC  >  NC_014034/2640088‑2640285

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG