Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,191 G→C 15.0% intergenic (‑167/+109) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1910G→C15.0% 43.1 ‑3.5 0.0131intergenic (‑167/+109)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/6):  ref base (48/63):  total (62/69)
Fisher's exact test strand distribution p-value = 3.11e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

ACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640092‑2640291
                                                                                                       |                                                                                                    
aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTgg                                                                                                      <  2:2249054/101‑1 (MQ=255)
 cgcgGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGa                                                                                                     <  1:3522607/101‑1 (MQ=255)
  gcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGag                                                                                                    <  1:1966406/101‑2 (MQ=255)
   cggcggCAGGCGTGTCAACCGGGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGagc                                                                                                   <  1:758480/101‑3 (MQ=25)
    ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  2:816660/101‑1 (MQ=37)
    ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  2:2035462/101‑1 (MQ=37)
    ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGAAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  1:3662671/101‑1 (MQ=25)
       ggcAGGCGTGTCAACCGTGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:694869/101‑1 (MQ=25)
        gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCCGCCCCCCCC.CTTC.CTTCCTGCCAATC.CTCCCCCCCCGCGATTTGGAGCaaaaa                                                                                              >  1:1838174/1‑98 (MQ=255)
         cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATg                                                                                             <  2:2116866/101‑1 (MQ=37)
          aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAATATGa                                                                                            <  2:4096019/101‑1 (MQ=25)
           ggggttccacccGTGGCACTTTTGCCAAGGC.AAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  2:3804816/91‑1 (MQ=17)
           ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  1:1203772/101‑1 (MQ=25)
            gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  2:3662876/101‑1 (MQ=25)
                   aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  2:2487968/101‑1 (MQ=21)
                   aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  1:3137833/101‑1 (MQ=25)
                     ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                <  1:1451997/101‑1 (MQ=25)
                            gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                         <  1:2672922/101‑1 (MQ=25)
                             cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                        <  1:3089224/101‑1 (MQ=21)
                              ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       <  2:3042280/101‑1 (MQ=25)
                                 tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                    <  1:3837973/101‑1 (MQ=21)
                                  gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   <  1:251102/101‑1 (MQ=25)
                                   cgcAGGCAAAAGGGGGGGGCGGCCCCCCCCTTTGGCCTT.CGGCCAATTACCCCCCCCGGGGT.TTTGG.GGAAGAAGAAAAAACAAGGGGTT.ATCTTTCCTCa                                                                  >  2:1067304/1‑101 (MQ=255)
                                   cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                  <  1:925952/101‑1 (MQ=25)
                                     cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                <  2:1433926/101‑1 (MQ=25)
                                       cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             <  1:536724/97‑1 (MQ=17)
                                       cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                             >  2:1809181/5‑101 (MQ=9)
                                        gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  2:1115952/101‑1 (MQ=25)
                                         cAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                            >  2:3948178/1‑101 (MQ=21)
                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:1086767/100‑1 (MQ=17)
                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:2171381/100‑1 (MQ=17)
                                           aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                         >  1:131524/1‑101 (MQ=17)
                                              ggggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       >  1:1356940/1‑101 (MQ=21)
                                              ggggggCCCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       <  2:2338473/101‑1 (MQ=25)
                                              agggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      >  2:4098878/2‑101 (MQ=18)
                                              agggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      >  1:529732/2‑101 (MQ=18)
                                               gggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     >  2:1643891/1‑101 (MQ=18)
                                               gggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                      <  2:1443836/101‑1 (MQ=25)
                                                ggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  2:4027452/1‑101 (MQ=18)
                                                ggggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  2:2878552/1‑101 (MQ=18)
                                                 gggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtg                                                   >  1:4120356/1‑99 (MQ=18)
                                                 gggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                    <  1:3920565/101‑1 (MQ=25)
                                                  ggCGCTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                   <  2:2075963/101‑1 (MQ=25)
                                                   gcgcTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  2:3270389/101‑5 (MQ=21)
                                                   gcgcTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  <  2:3909766/101‑1 (MQ=25)
                                                    cgcTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                 <  2:3468420/101‑1 (MQ=25)
                                                     gcTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               >  2:3670734/1‑95 (MQ=21)
                                                     gcTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGNGNGtn                                                >  1:1363739/1‑100 (MQ=25)
                                                      cTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGGGAAATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                              >  1:960724/1‑94 (MQ=25)
                                                      cTGCCCCCCTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               >  2:236615/1‑101 (MQ=25)
                                                        gCCCCCCTC..TGG.CTT.C..CCCATT.CCCCCCCCGGGGTATTTCGGGCAAGATGAAAGGCATCCGGTTTCATCTTGCTGCAAATATCCCGGGGGGAGTccgcttg                                          <  1:3647441/101‑6 (MQ=17)
                                                          cccccTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           <  1:14955/101‑1 (MQ=25)
                                                          cccccTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           >  2:3092790/1‑101 (MQ=37)
                                                           ccccTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          <  1:3978114/101‑1 (MQ=37)
                                                            tcttc.tTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         <  1:337064/98‑1 (MQ=25)
                                                            cccTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  1:1352283/1‑101 (MQ=37)
                                                            cccTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  1:1208728/1‑101 (MQ=37)
                                                             ccTC.TTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                        >  2:2793688/1‑101 (MQ=37)
                                                              ctc.tTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  1:336164/1‑101 (MQ=37)
                                                               tc.tTGGCCTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      >  1:2479405/1‑101 (MQ=37)
                                                               tc.tTGGCCTT.CGGCCAATT.CAACCCCCGAGGAATTTGGAGCAAGATG.AAAGACCATGGGTTAATCTTGAATCAAATATCCCGGGGGGAGTCCTGAAAgaga                                      >  2:337013/1‑98 (MQ=25)
                                                                  ttggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  1:144686/6‑101 (MQ=37)
                                                                   tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:3496281/5‑101 (MQ=37)
                                                                   tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:4197583/5‑101 (MQ=37)
                                                                    ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  >  1:2898838/4‑101 (MQ=37)
                                                                    ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  <  1:1280080/98‑1 (MQ=37)
                                                                      ccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                >  2:468205/2‑101 (MQ=37)
                                                                       cTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  2:1764107/1‑101 (MQ=37)
                                                                       cTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  1:824355/1‑101 (MQ=37)
                                                                        tt.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                              >  2:4017796/1‑101 (MQ=37)
                                                                        ctgcGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               <  1:886318/98‑1 (MQ=37)
                                                                           cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  2:3534400/1‑101 (MQ=255)
                                                                           cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  1:3003586/1‑101 (MQ=255)
                                                                            ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           <  1:3158304/101‑1 (MQ=255)
                                                                             gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                          >  2:490034/1‑101 (MQ=37)
                                                                             gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                          >  1:1952819/1‑101 (MQ=37)
                                                                               cAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  1:3660143/1‑101 (MQ=37)
                                                                                aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:3597345/1‑101 (MQ=37)
                                                                                aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       <  2:67344/101‑1 (MQ=255)
                                                                                 aTT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      <  2:463192/101‑1 (MQ=25)
                                                                                     caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:4042349/3‑101 (MQ=255)
                                                                                     caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:1520481/3‑101 (MQ=255)
                                                                                     caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  1:1174196/3‑101 (MQ=37)
                                                                                      accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  >  1:4120075/2‑101 (MQ=255)
                                                                                       ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 >  1:2928867/1‑101 (MQ=255)
                                                                                         ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               <  1:3093020/101‑1 (MQ=255)
                                                                                         ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               <  1:1709259/101‑1 (MQ=255)
                                                                                          cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg              >  2:2605875/1‑101 (MQ=255)
                                                                                            cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            <  1:3948438/101‑1 (MQ=255)
                                                                                             gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  1:2761305/1‑101 (MQ=255)
                                                                                             gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:1804722/1‑101 (MQ=255)
                                                                                             gAGGTAGTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           <  1:2254934/101‑1 (MQ=255)
                                                                                              aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          <  1:2793907/101‑1 (MQ=255)
                                                                                              aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:2040531/1‑101 (MQ=255)
                                                                                               ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         <  2:2878654/101‑1 (MQ=255)
                                                                                                gTATTTGGAGCAAGATG.AAAGACCATGCGTTCATCTGGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        <  1:1785083/101‑1 (MQ=25)
                                                                                                   tttGGAGCACGATG.AAAGACCATGGGATCATCTTGCCTCAAATAGCCCGGGGGAAGTCCTGAAAGGACGCGGTGCTGACCCCCCCACCCCACTCTTTTTCa     >  2:752225/1‑101 (MQ=16)
                                                                                                    ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  1:3191625/1‑101 (MQ=255)
                                                                                                     tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   <  1:2451474/101‑1 (MQ=255)
                                                                                                      ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:1490583/1‑101 (MQ=255)
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ACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640092‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG