Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,192 A→G 16.4% intergenic (‑168/+108) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1920A→G16.4% 45.2 ‑2.3 ‑0.0128intergenic (‑168/+108)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/7):  ref base (49/58):  total (63/65)
Fisher's exact test strand distribution p-value = 9.74e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 139 total aligned reads displayed.

CGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640093‑2640291
                                                                                                        |                                                                                                   
cgcgGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGa                                                                                                     <  1:3522607/101‑1 (MQ=255)
 gcggcgGGGGGCGTTTCGACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGGTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGag                                                                                                    <  1:1345409/101‑2 (MQ=21)
 gcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGag                                                                                                    <  1:1966406/101‑2 (MQ=255)
  cggcggCAGGCGTGTCAACCGGGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGagc                                                                                                   <  1:758480/101‑3 (MQ=25)
   ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  2:816660/101‑1 (MQ=37)
   ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGAAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  1:3662671/101‑1 (MQ=25)
      ggcAGGCGTGTCAACCGTGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:694869/101‑1 (MQ=25)
       gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCCGCCCCCCCC.CTTC..CTTCCTGCCAATC.CTCCCCCCCCGCGATTTGGAGCaaaaa                                                                                              >  1:1838174/1‑98 (MQ=255)
         aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAATATGa                                                                                            <  2:4096019/101‑1 (MQ=25)
          ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  1:1203772/101‑1 (MQ=25)
           gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  2:3662876/101‑1 (MQ=25)
               gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                      <  2:4056931/101‑5 (MQ=21)
                  aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  2:2487968/101‑1 (MQ=21)
                   aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                 <  1:3180511/101‑1 (MQ=25)
                    ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                <  1:1451997/101‑1 (MQ=25)
                           gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                         <  1:2672922/101‑1 (MQ=25)
                             ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       <  2:3042280/101‑1 (MQ=25)
                                tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                    <  1:3837973/101‑1 (MQ=21)
                                 gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   <  1:251102/101‑1 (MQ=25)
                                  cgcAGGCAAAAGGGGGGGGCGGCCCCCCCCTTTGGC.CTT.CGGCCAATTACCCCCCCCGGGGT.TTTGG.GGAAGAAGAAAAAACAAGGGGTT.ATCTTTCCTCa                                                                  >  2:1067304/1‑101 (MQ=255)
                                  cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                  <  1:925952/101‑1 (MQ=25)
                                    cAGGCAAAAGGGGGGGGCCGCCCCCCCC.TTTGCCCTT.GGGCCAATT.ACCCCCCCG.GGGATTTTGGGGAAAATGAAAA.ACCATGGGTTTATCTTTCCTCaaa                                                                >  2:2451290/1‑101 (MQ=255)
                                    cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                <  2:1433926/101‑1 (MQ=25)
                                      cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             <  1:536724/97‑1 (MQ=17)
                                      cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                             >  2:1809181/5‑101 (MQ=9)
                                       gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  2:1115952/101‑1 (MQ=25)
                                        cAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                            >  2:3948178/1‑101 (MQ=21)
                                         gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:2171381/100‑1 (MQ=17)
                                         gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          >  1:2592976/2‑101 (MQ=17)
                                         gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:1086767/100‑1 (MQ=17)
                                          aaaGGGGGGAGCTGCCCCCCTC.TTGGC.CTT.TGGCCAATT.CACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATacc                                                          >  2:551273/1‑101 (MQ=18)
                                          aaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                         >  1:131524/1‑101 (MQ=17)
                                            aagggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                       >  2:1863998/3‑101 (MQ=18)
                                             ggggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       <  2:211406/101‑1 (MQ=25)
                                             ggggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       >  1:1356940/1‑101 (MQ=21)
                                             ggggggCCCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       <  2:2338473/101‑1 (MQ=25)
                                             agggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      >  2:4098878/2‑101 (MQ=18)
                                             agggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      >  1:529732/2‑101 (MQ=18)
                                              gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     <  1:1490286/101‑1 (MQ=18)
                                              gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     >  2:1643891/1‑101 (MQ=18)
                                              gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                      <  2:1443836/101‑1 (MQ=25)
                                               ggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                    >  2:3483856/1‑100 (MQ=18)
                                               ggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  2:4027452/1‑101 (MQ=18)
                                               ggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  2:2878552/1‑101 (MQ=18)
                                                gggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtg                                                   >  1:4120356/1‑99 (MQ=18)
                                                 ggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                   <  2:2075963/101‑1 (MQ=25)
                                                  gcgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  2:3270389/101‑5 (MQ=21)
                                                  gcgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  <  2:3909766/101‑1 (MQ=25)
                                                   cgcTGCCCCCCTC.TTGGC.CTT.CGGCCCATT.CACCCCCCCAGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCCCAAATATCCCcgggggggg                                                 >  1:3457657/1‑98 (MQ=21)
                                                    gcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               >  2:3670734/1‑95 (MQ=21)
                                                    gcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGNGNGtn                                                >  1:1363739/1‑100 (MQ=25)
                                                     cTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGAAATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                              >  1:960724/1‑94 (MQ=25)
                                                       gCCCCCCTC..TGG..CTT.C..CCCATT.CCCCCCCCGGGGTATTTCGGGCAAGATGAAAGGCATCCGGTTTCATCTTGCTGCAAATATCCCGGGGGGAGTccgcttg                                          <  1:3647441/101‑6 (MQ=17)
                                                         cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           >  1:313449/1‑101 (MQ=37)
                                                         cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           <  1:14955/101‑1 (MQ=25)
                                                          ccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          <  1:3978114/101‑1 (MQ=37)
                                                           tcttc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         <  1:337064/98‑1 (MQ=25)
                                                           cccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  1:1208728/1‑101 (MQ=37)
                                                            ccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                        >  2:2793688/1‑101 (MQ=37)
                                                             ctc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  1:3750789/1‑101 (MQ=37)
                                                              tc.tTGGC.CTT.CGGCCAATT.CAACCCCCGAGGAATTTGGAGCAAGATG.AAAGACCATGGGTTAATCTTGAATCAAATATCCCGGGGGGAGTCCTGAAAgaga                                      >  2:337013/1‑98 (MQ=25)
                                                                  ttggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  1:144686/6‑101 (MQ=37)
                                                                   tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACagg                                   >  2:3866125/5‑101 (MQ=25)
                                                                   tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:4197583/5‑101 (MQ=37)
                                                                    ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  <  1:1280080/98‑1 (MQ=37)
                                                                     gtcTT.CGTCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 <  1:2980666/99‑1 (MQ=37)
                                                                      ccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                >  2:468205/2‑101 (MQ=37)
                                                                       cTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  1:824355/1‑101 (MQ=37)
                                                                        tt.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                              >  2:4017796/1‑101 (MQ=37)
                                                                        ctgcGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               <  1:886318/98‑1 (MQ=37)
                                                                           cGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            <  1:1922506/101‑1 (MQ=255)
                                                                           cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  1:3003586/1‑101 (MQ=255)
                                                                           cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  2:3534400/1‑101 (MQ=255)
                                                                            ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  1:2892209/1‑101 (MQ=37)
                                                                            ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           <  1:3158304/101‑1 (MQ=255)
                                                                              ccAATT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                         <  2:3002284/101‑1 (MQ=255)
                                                                               cAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  2:1709144/1‑101 (MQ=255)
                                                                                aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       <  2:67344/101‑1 (MQ=255)
                                                                                aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:3597345/1‑101 (MQ=37)
                                                                                 aTT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      <  2:463192/101‑1 (MQ=25)
                                                                                 aTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  1:2473321/1‑101 (MQ=37)
                                                                                    tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    <  2:3330312/98‑1 (MQ=255)
                                                                                     caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:1520481/3‑101 (MQ=255)
                                                                                     caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  1:1174196/3‑101 (MQ=37)
                                                                                      accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  >  1:4120075/2‑101 (MQ=255)
                                                                                       ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 >  1:2928867/1‑101 (MQ=255)
                                                                                        cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  1:3620756/101‑1 (MQ=255)
                                                                                         ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               <  1:1709259/101‑1 (MQ=255)
                                                                                         ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               <  1:3093020/101‑1 (MQ=255)
                                                                                          cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg              <  2:225005/101‑1 (MQ=255)
                                                                                            cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            <  1:3948438/101‑1 (MQ=255)
                                                                                             gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:2863901/1‑101 (MQ=255)
                                                                                             gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:1804722/1‑101 (MQ=255)
                                                                                             gAGGTAGTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           <  1:2254934/101‑1 (MQ=255)
                                                                                              aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          <  1:2793907/101‑1 (MQ=255)
                                                                                              aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:2040531/1‑101 (MQ=255)
                                                                                                gTATTTGGAGCAAGATG.AAAGACCATGCGTTCATCTGGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        <  1:1785083/101‑1 (MQ=25)
                                                                                                   tttGGAGCACGATG.AAAGACCATGGGATCATCTTGCCTCAAATAGCCCGGGGGAAGTCCTGAAAGGACGCGGTGCTGACCCCCCCACCCCACTCTTTTTCa     >  2:752225/1‑101 (MQ=16)
                                                                                                    ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  1:3191625/1‑101 (MQ=255)
                                                                                                     tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   <  1:2451474/101‑1 (MQ=255)
                                                                                                        |                                                                                                   
CGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640093‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG