Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,193 A→G 100% intergenic (‑169/+107) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1930A→G100.0% 418.2 128intergenic (‑169/+107)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (63/65):  ref base (0/0):  total (63/65)

Only 100 of 135 total aligned reads displayed.

GGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640096‑2640291
                                                                                                      |                                                                                                  
ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  2:816660/101‑1 (MQ=37)
ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  2:2035462/101‑1 (MQ=37)
ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGAAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCa                                                                                                  <  1:3662671/101‑1 (MQ=25)
   ggcAGGCGTGTCAACCGTGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:694869/101‑1 (MQ=25)
    gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCCGCCCCCCCC.CTTC..CTTCCTGCCAATC.CTCCCCCCCCGCGATTTGGAGCaaaaa                                                                                              >  1:1838174/1‑98 (MQ=255)
      aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAATATGa                                                                                            <  2:4096019/101‑1 (MQ=25)
       ggggttccacccGTGGCACTTTTGCCAAGGC.AAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  2:3804816/91‑1 (MQ=17)
       ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  1:1203772/101‑1 (MQ=25)
         cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                         <  1:1732739/101‑2 (MQ=25)
            gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                      <  2:4056931/101‑5 (MQ=21)
               aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  2:2487968/101‑1 (MQ=21)
               aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  1:3137833/101‑1 (MQ=25)
                 ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CTGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                <  2:3662767/101‑1 (MQ=25)
                      gcgcTTCTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                           <  1:685582/101‑1 (MQ=21)
                        gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                         <  1:2672922/101‑1 (MQ=25)
                         cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                        <  1:3089224/101‑1 (MQ=21)
                          ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       <  2:3042280/101‑1 (MQ=25)
                              gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   <  1:251102/101‑1 (MQ=25)
                               cgcAGGCAAAAGGGGGGGGCGGCCCCCCCCTTTGGC.CTT.CGGCCAATTACCCCCCCCGGGGT.TTTGG.GGAAGAAGAAAAAACAAGGGGTT.ATCTTTCCTCa                                                                  >  2:1067304/1‑101 (MQ=255)
                               cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                  <  1:925952/101‑1 (MQ=25)
                                 gccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                               >  1:1398393/7‑101 (MQ=14)
                                 cAGGCAAAAGGGGGGGGCCGCCCCCCCC.TTTGCCCTT.GGGCCAATT.ACCCCCCCG.GGGATTTTGGGGAAAATGAAAA.ACCATGGGTTTATCTTTCCTCaaa                                                                >  2:2451290/1‑101 (MQ=255)
                                 cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                <  2:1433926/101‑1 (MQ=25)
                                   ggCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                              <  1:210318/101‑1 (MQ=21)
                                   cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             <  1:536724/97‑1 (MQ=17)
                                   cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                             >  2:1809181/5‑101 (MQ=9)
                                    gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  2:3949620/101‑1 (MQ=25)
                                     cAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                            >  2:3948178/1‑101 (MQ=21)
                                      gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:2171381/100‑1 (MQ=17)
                                      gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:1086767/100‑1 (MQ=17)
                                      gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          >  1:2592976/2‑101 (MQ=17)
                                       aaaGGGGGGAGCTGCCCCCCTC.TTGGC.CTT.TGGCCAATT.CACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATacc                                                          >  2:551273/1‑101 (MQ=18)
                                       aaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                         >  1:131524/1‑101 (MQ=17)
                                          ggggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       <  2:211406/101‑1 (MQ=25)
                                          ggggggCCCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       <  2:2338473/101‑1 (MQ=25)
                                          agggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      >  1:529732/2‑101 (MQ=18)
                                           gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     <  1:1490286/101‑1 (MQ=18)
                                           gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     >  2:1643891/1‑101 (MQ=18)
                                           gggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                      <  2:1443836/101‑1 (MQ=25)
                                            ggggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  2:2878552/1‑101 (MQ=18)
                                             gggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtg                                                   >  1:4120356/1‑99 (MQ=18)
                                             gggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                    <  1:3920565/101‑1 (MQ=25)
                                              ggCGCTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                   <  2:2075963/101‑1 (MQ=25)
                                               gcgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  2:3270389/101‑5 (MQ=21)
                                                cgcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                 <  2:3468420/101‑1 (MQ=25)
                                                 gcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               >  2:3670734/1‑95 (MQ=21)
                                                 gcTGCCCCCCTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGNGNGtn                                                >  1:1363739/1‑100 (MQ=25)
                                                    gCCCCCCTC..TGG..CTT.C..CCCATT.CCCCCCCCGGGGTATTTCGGGCAAGATGAAAGGCATCCGGTTTCATCTTGCTGCAAATATCCCGGGGGGAGTccgcttg                                          <  1:3647441/101‑6 (MQ=17)
                                                      cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           >  2:3092790/1‑101 (MQ=37)
                                                      cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           >  1:313449/1‑101 (MQ=37)
                                                      cccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           <  1:14955/101‑1 (MQ=25)
                                                       ccccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          <  1:3978114/101‑1 (MQ=37)
                                                        tcttc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         <  1:337064/98‑1 (MQ=25)
                                                        cccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  1:1208728/1‑101 (MQ=37)
                                                        cccTC.TTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  1:1352283/1‑101 (MQ=37)
                                                          ctc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  1:336164/1‑101 (MQ=37)
                                                          ctc.tTGGC.CTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  1:3750789/1‑101 (MQ=37)
                                                           tc.tTGGC.CTT.CGGCCAATT.CAACCCCCGAGGAATTTGGAGCAAGATG.AAAGACCATGGGTTAATCTTGAATCAAATATCCCGGGGGGAGTCCTGAAAgaga                                      >  2:337013/1‑98 (MQ=25)
                                                               ttggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    <  2:1208634/96‑1 (MQ=37)
                                                               ttggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  1:144686/6‑101 (MQ=37)
                                                                tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:3496281/5‑101 (MQ=37)
                                                                tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACagg                                   >  2:3866125/5‑101 (MQ=25)
                                                                tggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                   >  1:4197583/5‑101 (MQ=37)
                                                                 ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  <  1:1280080/98‑1 (MQ=37)
                                                                 ggccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  >  1:2898838/4‑101 (MQ=37)
                                                                  gtcTT.CGTCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 <  1:2980666/99‑1 (MQ=37)
                                                                   ccTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                >  2:468205/2‑101 (MQ=37)
                                                                    cTT.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  2:1764107/1‑101 (MQ=37)
                                                                     tt.CGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                              >  2:4017796/1‑101 (MQ=37)
                                                                     ctgcGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               <  1:886318/98‑1 (MQ=37)
                                                                        cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  2:3534400/1‑101 (MQ=255)
                                                                        cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  1:907509/1‑101 (MQ=37)
                                                                        cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  1:3003586/1‑101 (MQ=255)
                                                                         ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  1:2892209/1‑101 (MQ=37)
                                                                         ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           <  1:3158304/101‑1 (MQ=255)
                                                                          gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                          >  1:1952819/1‑101 (MQ=37)
                                                                             aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       <  2:67344/101‑1 (MQ=255)
                                                                             aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:3597345/1‑101 (MQ=37)
                                                                              aTT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      <  2:463192/101‑1 (MQ=25)
                                                                              aTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  1:2473321/1‑101 (MQ=37)
                                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:4042349/3‑101 (MQ=255)
                                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:1520481/3‑101 (MQ=255)
                                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  1:1174196/3‑101 (MQ=37)
                                                                                   accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  >  1:4120075/2‑101 (MQ=255)
                                                                                     cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  1:3620756/101‑1 (MQ=255)
                                                                                       cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg              >  2:2605875/1‑101 (MQ=255)
                                                                                       cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg              <  2:225005/101‑1 (MQ=255)
                                                                                         cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            <  1:3948438/101‑1 (MQ=255)
                                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:2863901/1‑101 (MQ=255)
                                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:1804722/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:2040531/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          <  1:2793907/101‑1 (MQ=255)
                                                                                            ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         <  2:2878654/101‑1 (MQ=255)
                                                                                             gTATTTGGAGCAAGATG.AAAGACCATGCGTTCATCTGGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        <  1:1785083/101‑1 (MQ=25)
                                                                                               aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:4159715/1‑101 (MQ=255)
                                                                                                tttGGAGCACGATG.AAAGACCATGGGATCATCTTGCCTCAAATAGCCCGGGGGAAGTCCTGAAAGGACGCGGTGCTGACCCCCCCACCCCACTCTTTTTCa     >  2:752225/1‑101 (MQ=16)
                                                                                                 ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  1:966126/1‑101 (MQ=255)
                                                                                                 ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  1:3191625/1‑101 (MQ=255)
                                                                                                  tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   <  1:2451474/101‑1 (MQ=255)
                                                                                                   ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:1490583/1‑101 (MQ=255)
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GGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTT.CGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640096‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG