Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,210 T→G 14.2% intergenic (‑186/+90) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2100T→G14.2% 48.2 ‑1.5 ‑0.0141intergenic (‑186/+90)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/6):  ref base (66/54):  total (80/61)
Fisher's exact test strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 150 total aligned reads displayed.

AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTTCGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640111‑2640310
                                                                                                       |                                                                                                    
aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                     <  2:2487968/101‑1 (MQ=21)
 aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                    <  1:3180511/101‑1 (MQ=25)
  ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                                   <  1:1451997/101‑1 (MQ=25)
       gcgcTTCTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                              <  1:685582/101‑1 (MQ=21)
           ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                          <  2:3042280/101‑1 (MQ=25)
                cgcAGGCAAAAGGGGGGGGCGGCCCCCCCCTTTGGC.CTTCGGCCAATTACCCCCCCCGGGGT.TTTGG.GGAAGAAGAAAAAACAAGGGGTT.ATCTTTCCTCa                                                                                     >  2:1067304/1‑101 (MQ=255)
                cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                                     <  1:925952/101‑1 (MQ=25)
                  cAGGCAAAAGGGGGGGGCCGCCCCCCCC.TTTGCCCTTGGGCCAATT.ACCCCCCCG.GGGATTTTGGGGAAAATGAAAA.ACCATGGGTTTATCTTTCCTCaaa                                                                                   >  2:2451290/1‑101 (MQ=255)
                  cAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                                   <  2:1433926/101‑1 (MQ=25)
                    cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGCTTATCTTGCCCCAAatat                                                                                >  2:1809181/5‑101 (MQ=9)
                     gCAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                                <  2:3949620/101‑1 (MQ=25)
                      cAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                               <  1:4044003/101‑1 (MQ=25)
                      cAAAAGGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                               >  2:3948178/1‑101 (MQ=21)
                       gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             <  2:2171381/100‑1 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             <  2:1086767/100‑1 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             >  1:2592976/2‑101 (MQ=17)
                        aaaGGGGGGAGCTGCCCCCCTC.TTGGC.CTTTGGCCAATT.CACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATacc                                                                             >  2:551273/1‑101 (MQ=18)
                        aaaAGGGGGCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCg                                                                            >  1:131524/1‑101 (MQ=17)
                          aagggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                          >  2:1863998/3‑101 (MQ=18)
                           ggggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                                          <  2:211406/101‑1 (MQ=25)
                           ggggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                                          >  1:1356940/1‑101 (MQ=21)
                           agggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                         >  1:529732/2‑101 (MQ=18)
                           agggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                         >  2:4098878/2‑101 (MQ=18)
                             ggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                                       >  2:2878552/1‑101 (MQ=18)
                             ggggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                                       >  2:4027452/1‑101 (MQ=18)
                              gggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                       <  1:3920565/101‑1 (MQ=25)
                               ggCGCTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                      <  2:2075963/101‑1 (MQ=25)
                                gcgcTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                    <  2:3270389/101‑5 (MQ=21)
                                 cgcTGCCCCCCTC.TTGGC.CTTCGGCCCATT.CACCCCCCCAGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCCCAAATATCCCcgggggggg                                                                    >  1:3457657/1‑98 (MQ=21)
                                 cgcTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                                    <  2:3468420/101‑1 (MQ=25)
                                  gcTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                  >  2:3670734/1‑95 (MQ=21)
                                  gcTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGNGNGtn                                                                   >  1:1363739/1‑100 (MQ=25)
                                   cTGCCCCCCTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                  >  2:236615/1‑101 (MQ=25)
                                     gCCCCCCTC..TGG..CTTC..CCCATT.CCCCCCCCGGGGTATTTCGGGCAAGATGAAAGGCATCCGGTTTCATCTTGCTGCAAATATCCCGGGGGGAGTccgcttg                                                             <  1:3647441/101‑6 (MQ=17)
                                       cccccTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                              >  1:313449/1‑101 (MQ=37)
                                       cccccTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                              <  1:14955/101‑1 (MQ=25)
                                         tcttc.tTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                            <  1:337064/98‑1 (MQ=25)
                                         cccTC.TTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                            >  1:1208728/1‑101 (MQ=37)
                                           ctc.tTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                          >  1:3750789/1‑101 (MQ=37)
                                           ctc.tTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                          >  1:336164/1‑101 (MQ=37)
                                            tc.tTGGC.CTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                         >  1:2479405/1‑101 (MQ=37)
                                                ttggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                       >  1:144686/6‑101 (MQ=37)
                                                 tggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                      >  1:3496281/5‑101 (MQ=37)
                                                 tggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACagg                                                      >  2:3866125/5‑101 (MQ=25)
                                                 tggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGAAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                      >  1:4197583/5‑101 (MQ=37)
                                                  ggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                                     >  1:2898838/4‑101 (MQ=37)
                                                  ggccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                                     <  1:1280080/98‑1 (MQ=37)
                                                    ccTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                                   >  2:468205/2‑101 (MQ=37)
                                                     ctgcGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCGTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                  <  1:886318/98‑1 (MQ=37)
                                                     cTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                  >  2:1764107/1‑101 (MQ=37)
                                                     cTTCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                  >  1:824355/1‑101 (MQ=37)
                                                      ttCGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                                 >  2:4017796/1‑101 (MQ=37)
                                                        cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                               >  2:3534400/1‑101 (MQ=255)
                                                        cGGCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                               >  1:3003586/1‑101 (MQ=255)
                                                         ggCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                              <  1:3158304/101‑1 (MQ=255)
                                                          gCCAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                             >  1:1952819/1‑101 (MQ=37)
                                                           ccAATT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                            <  2:3002284/101‑1 (MQ=255)
                                                            cAATT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                           >  2:1709144/1‑101 (MQ=255)
                                                             aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          >  2:3597345/1‑101 (MQ=37)
                                                             aaTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          <  2:67344/101‑1 (MQ=255)
                                                              aTT.CGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGCCCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         <  2:463192/101‑1 (MQ=25)
                                                              aTT.CACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         >  1:2473321/1‑101 (MQ=37)
                                                                 tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                       <  2:3330312/98‑1 (MQ=255)
                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                      >  2:1520481/3‑101 (MQ=255)
                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGAGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                      >  1:1174196/3‑101 (MQ=37)
                                                                   accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                     >  1:4120075/2‑101 (MQ=255)
                                                                    ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                    >  1:2928867/1‑101 (MQ=255)
                                                                     cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGCTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                   <  1:3620756/101‑1 (MQ=255)
                                                                      ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                  <  1:1709259/101‑1 (MQ=255)
                                                                      ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                  <  1:3093020/101‑1 (MQ=255)
                                                                       cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                 <  2:225005/101‑1 (MQ=255)
                                                                       cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                 >  2:2605875/1‑101 (MQ=255)
                                                                         cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                               <  1:3948438/101‑1 (MQ=255)
                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              >  1:2761305/1‑101 (MQ=255)
                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              >  2:2863901/1‑101 (MQ=255)
                                                                          gAGGTAGTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              <  1:2254934/101‑1 (MQ=255)
                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                             >  1:2040531/1‑101 (MQ=255)
                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                             <  1:2793907/101‑1 (MQ=255)
                                                                            ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                            >  2:1466929/1‑101 (MQ=255)
                                                                             gTATTTGGAGCAAGATG.AAAGACCATGCGTTCATCTGGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                           <  1:1785083/101‑1 (MQ=25)
                                                                                tttGGAGCACGATG.AAAGACCATGGGATCATCTTGCCTCAAATAGCCCGGGGGAAGTCCTGAAAGGACGCGGTGCTGACCCCCCCACCCCACTCTTTTTCa                        >  2:752225/1‑101 (MQ=16)
                                                                                 ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                       >  1:966126/1‑101 (MQ=255)
                                                                                   ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                     >  1:1490583/1‑101 (MQ=255)
                                                                                    gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg                    >  2:1188532/4‑101 (MQ=255)
                                                                                     agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt                   >  2:4107679/3‑101 (MQ=255)
                                                                                       cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                 <  1:551337/101‑1 (MQ=255)
                                                                                        aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                <  2:207554/101‑1 (MQ=255)
                                                                                             tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt            <  1:490091/99‑1 (MQ=255)
                                                                                              gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           <  1:2625931/100‑1 (MQ=255)
                                                                                               aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  1:2226410/1‑101 (MQ=255)
                                                                                               aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  2:1184025/1‑101 (MQ=255)
                                                                                               aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  2:3093698/1‑101 (MQ=255)
                                                                                               aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  1:432620/1‑101 (MQ=255)
                                                                                                aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg         >  2:2331731/1‑101 (MQ=255)
                                                                                                  gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa       <  2:1356840/101‑1 (MQ=255)
                                                                                                     cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg    >  2:1003212/1‑101 (MQ=255)
                                                                                                      aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg   >  1:3725665/1‑101 (MQ=255)
                                                                                                      aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAGAACCgg   >  1:3024195/1‑101 (MQ=255)
                                                                                                       tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa  >  1:941495/1‑101 (MQ=255)
                                                                                                       tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa  >  2:838164/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG..CTTCGGCCAATT.CGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640111‑2640310

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG