Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,715,979 A→G 100% F8L (TTC→CTC)  amaB ← N‑carbamoyl‑L‑amino acid amidohydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,715,9790A→G100.0% 862.2 231F8L (TTC→CTC) amaBN‑carbamoyl‑L‑amino acid amidohydrolase
Reads supporting (aligned to +/- strand):  new base (104/126):  ref base (0/0):  total (104/127)

Only 100 of 236 total aligned reads displayed.

ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076
                                                                                                  |                                                                                                 
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 <  1:4083342/101‑1 (MQ=255)
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  2:4055413/1‑101 (MQ=255)
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGAGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  2:3014603/1‑101 (MQ=255)
  cggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGttt                                                                                               <  2:1164598/101‑1 (MQ=255)
  cggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGttt                                                                                               >  1:2137211/1‑101 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              <  2:1076462/101‑1 (MQ=255)
    gTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtc                                                                                             <  1:3238154/101‑1 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtct                                                                                            >  1:1346820/1‑101 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtct                                                                                            <  1:2266715/101‑1 (MQ=255)
      cACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtctc                                                                                           <  1:1727026/101‑1 (MQ=255)
       aCGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTcc                                                                                          <  1:595297/101‑1 (MQ=255)
        cGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         >  2:1005251/1‑101 (MQ=255)
        cGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         <  1:2609947/101‑1 (MQ=255)
        cGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCg                                                                                         >  2:3547230/1‑101 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGTGATCCCCGGCCCGACCTGCGCGTTCTTCATGAGGATGTCACACAGCAGGGCTGCCTTGAGACGGTGGTTTTCTCCgg                                                                                        >  2:1667951/1‑101 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCgg                                                                                        >  1:4029446/1‑101 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCgg                                                                                        <  1:3036089/101‑1 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGACCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCgg                                                                                        <  1:3642461/101‑1 (MQ=255)
            cTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTg                                                                                     <  1:721396/101‑1 (MQ=255)
              ggCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGAt                                                                                   >  1:874807/1‑101 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 <  2:2137066/101‑1 (MQ=255)
                 ggTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGc                                                                                >  1:439406/1‑101 (MQ=255)
                   ttgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCAt                                                                              >  1:2962946/1‑101 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             >  2:1530899/1‑101 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             >  1:1193615/1‑101 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            <  1:3710924/101‑1 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  2:2372777/101‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          <  1:784500/101‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          >  1:2734732/1‑101 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          <  1:4214582/101‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGAGATGGCATCTgg                                                                          >  1:3410427/1‑101 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         <  2:1214263/101‑1 (MQ=255)
                         ccccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        >  1:3871288/1‑101 (MQ=255)
                         ccccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        >  1:1845885/1‑101 (MQ=255)
                         ccccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        <  1:2029255/101‑1 (MQ=255)
                           ccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGt                                                                      >  1:1572587/1‑101 (MQ=255)
                             ccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtct                                                                    <  1:93425/101‑1 (MQ=255)
                             ccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtct                                                                    >  1:2421579/1‑101 (MQ=255)
                                 gATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCt                                                                >  2:2943624/1‑101 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              <  1:2499976/101‑1 (MQ=255)
                                    ccccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtc                                                             >  1:2538252/1‑101 (MQ=255)
                                     cccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcg                                                            >  2:1861046/1‑101 (MQ=255)
                                       cGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcgtc                                                          <  1:3532360/101‑1 (MQ=255)
                                       cGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcgtc                                                          <  1:2681738/101‑1 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  2:284550/1‑101 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         <  1:1130800/101‑1 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  1:3830164/1‑101 (MQ=255)
                                         gCCCGACCTGCGCCATCTGCATGAGGCTGTCCCGCAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTg                                                        <  1:3670999/101‑1 (MQ=255)
                                           ccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCg                                                      <  2:3789941/101‑1 (MQ=255)
                                           ccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCg                                                      <  1:2050148/101‑1 (MQ=255)
                                             gACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCggg                                                    <  1:1668066/101‑1 (MQ=255)
                                                  gcgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  1:3577121/101‑1 (MQ=255)
                                                  gcgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  1:2493635/101‑1 (MQ=255)
                                                   cgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTg                                              <  2:1273587/101‑1 (MQ=255)
                                                   cgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTg                                              <  2:165565/101‑1 (MQ=255)
                                                    gcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGc                                             <  2:1588188/101‑1 (MQ=255)
                                                      cATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGccc                                           >  2:4146616/1‑101 (MQ=255)
                                                      cATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGccc                                           >  2:637307/1‑101 (MQ=255)
                                                      cATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGccc                                           >  2:3188258/1‑101 (MQ=255)
                                                        tCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctc                                         <  1:1899887/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  2:3867/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGAAGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  1:2094870/101‑1 (MQ=255)
                                                            cATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     >  2:247920/1‑101 (MQ=255)
                                                            cATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     <  1:1798727/101‑1 (MQ=255)
                                                             aTGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCg                                    >  2:1919604/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  2:927251/1‑101 (MQ=255)
                                                                 ggCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTc                                <  2:3883233/101‑1 (MQ=255)
                                                                  gCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCa                               <  2:2226808/101‑1 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            >  1:1628494/1‑101 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            >  2:654975/1‑101 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            >  1:540654/1‑101 (MQ=255)
                                                                      tCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtttt                           >  1:284816/1‑101 (MQ=255)
                                                                       cccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTc                          <  1:1627380/101‑1 (MQ=255)
                                                                        ccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCt                         <  2:2734526/101‑1 (MQ=255)
                                                                          acaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGa                       <  2:3763155/101‑1 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     <  2:718114/101‑1 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     <  2:957909/101‑1 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     <  1:3133272/101‑1 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGAGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     >  2:2832033/1‑101 (MQ=255)
                                                                              gcgcgcGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGa                   <  1:484136/101‑1 (MQ=255)
                                                                                 cgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTg                <  2:3354568/101‑1 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               <  1:4075974/101‑1 (MQ=255)
                                                                                     gAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            <  2:2673408/101‑1 (MQ=255)
                                                                                     gAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            <  2:1107521/101‑1 (MQ=255)
                                                                                     gAATTGATACGGACGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            <  1:2832252/101‑1 (MQ=255)
                                                                                       aTTGATACGGAGGTTTTCTCCGGGTGATGGCAGCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCaga          <  2:1995315/101‑1 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        <  2:258128/101‑1 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  2:519166/1‑101 (MQ=255)
                                                                                          gATACGGAGGTTTTCTCCGGGGGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGa       >  2:1761465/1‑101 (MQ=255)
                                                                                            tACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaaa     >  1:1015045/1‑101 (MQ=255)
                                                                                            tACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaaa     >  2:1361957/1‑101 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTTGCTGTCTCCCTGGCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGGCAGAGGAAag    <  2:1712166/101‑1 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    >  2:3953775/1‑101 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    >  2:3129667/1‑101 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   <  2:439349/101‑1 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   >  2:483516/1‑101 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   <  1:1281089/101‑1 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   >  2:3104844/1‑101 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGCGGAAagag  >  2:2543722/1‑101 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAagag  >  2:3662874/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG