Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,731,800 G→C 100% P509P (CCC→CCG RCAP_rcc02540 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,731,8000G→C100.0% 654.5 176P509P (CCC→CCGRCAP_rcc02540diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (102/73):  ref base (0/0):  total (103/73)

Only 100 of 188 total aligned reads displayed.

CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGCC  >  NC_014034/2731705‑2731896
                                                                                               |                                                                                                
cAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcg                                                                                             >  2:1785075/1‑101 (MQ=255)
cAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcg                                                                                             >  2:3662402/1‑101 (MQ=255)
  gATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcg                                                                                           >  1:2429848/1‑101 (MQ=255)
    tCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcg                                                                                         <  2:1454445/101‑1 (MQ=255)
    tCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcg                                                                                         >  1:4003512/1‑101 (MQ=255)
      ccccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgcg                                                                                       <  2:1575930/100‑1 (MQ=255)
       cccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgcgc                                                                                      <  2:3182885/101‑1 (MQ=255)
        ccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCa                                                                                     <  2:4194429/101‑1 (MQ=255)
         cGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAg                                                                                    >  2:4070869/1‑101 (MQ=255)
           gTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCt                                                                                  >  1:3712784/1‑101 (MQ=255)
           gTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCt                                                                                  >  1:1678623/1‑101 (MQ=255)
           gTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCt                                                                                  >  1:862983/1‑101 (MQ=255)
             cGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgc                                                                                <  2:1843130/101‑1 (MQ=255)
                ccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGcc                                                                             <  1:1336794/101‑1 (MQ=255)
                 cAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCg                                                                            >  2:3306021/1‑101 (MQ=255)
                  aGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGATGCGCCgg                                                                           >  2:3462563/1‑101 (MQ=255)
                    cTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGc                                                                         <  2:2139734/101‑1 (MQ=255)
                     tGCGGGCCTAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                        >  1:493043/1‑101 (MQ=255)
                     tGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                        <  1:3420191/101‑1 (MQ=255)
                     tGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                        <  2:83861/101‑1 (MQ=255)
                     tGCAGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCGGCAAGAGATCCCCCGCCGCGGGCGCGCGGAGGTCCGCCGGCcc                                                                        >  2:2012341/1‑101 (MQ=255)
                      gCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCg                                                                       <  1:1870458/101‑1 (MQ=255)
                       cGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGa                                                                      >  2:3932668/1‑101 (MQ=255)
                       cGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGa                                                                      >  1:1587903/1‑101 (MQ=255)
                       cGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGa                                                                      >  2:754800/1‑101 (MQ=255)
                           ccGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTg                                                                  <  1:373490/101‑1 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGCCCTggg                                                                >  1:659277/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                                >  1:4083967/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                                >  2:193557/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                                >  1:2626183/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGACCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                                >  2:3512500/1‑101 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                               <  2:411993/101‑1 (MQ=255)
                               aaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgcg                                                              >  2:1443688/1‑101 (MQ=255)
                                aCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgcgc                                                             <  1:2474942/101‑1 (MQ=255)
                                 cGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGcgcg                                                            >  2:1354232/1‑101 (MQ=255)
                                 cGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGcgcg                                                            <  2:3870577/101‑1 (MQ=255)
                                  ggTCCTTTGTCTGCTGGAACTGGGCGAACGGCAGGAAAGCCTGCCGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCgg                                                           <  1:1015422/101‑1 (MQ=255)
                                   gTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGGCGACCTGGGCGCGGa                                                          >  1:2011354/1‑101 (MQ=255)
                                    tCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGaa                                                         <  2:3734191/101‑1 (MQ=255)
                                    tCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGaa                                                         <  1:4223762/101‑1 (MQ=255)
                                     ccTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAg                                                        >  1:1166052/1‑101 (MQ=255)
                                        tGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                     <  1:3987898/101‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                   <  1:2020404/101‑1 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                                 <  1:947084/101‑1 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                                 >  2:2275817/1‑101 (MQ=255)
                                             tgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgcg                                                >  2:1205647/1‑101 (MQ=255)
                                             tgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgcg                                                >  2:1212523/1‑101 (MQ=255)
                                             tgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgcg                                                >  1:1093339/1‑101 (MQ=255)
                                                tgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAtt                                             >  1:3816604/1‑101 (MQ=255)
                                                tgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAtt                                             >  1:1639921/1‑101 (MQ=255)
                                                  gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                           <  1:1209365/101‑1 (MQ=255)
                                                   aaCTGGTAGATCGACAGAAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTggg                                          <  2:2583819/101‑1 (MQ=255)
                                                     cTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCa                                        <  2:585904/101‑1 (MQ=255)
                                                      tGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAg                                       <  2:1882072/101‑1 (MQ=255)
                                                      tGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAg                                       >  1:1945260/1‑101 (MQ=255)
                                                      tGGTCGATCGACAGGAAGACCGGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGTCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAg                                       >  1:3116410/1‑101 (MQ=255)
                                                        gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                     >  1:2808339/1‑101 (MQ=255)
                                                         tcgatcgaCAGGAAGCGCTGCTGCAAGAGTTCCCCCGCCGGGCGCGCGCGCAGCTGCACCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                    <  2:953116/101‑1 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                    <  2:4011304/101‑1 (MQ=255)
                                                           gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  >  1:1177690/1‑101 (MQ=255)
                                                           gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  <  1:268536/101‑1 (MQ=255)
                                                            atcgaCCGGAAGACCAGCCGCACGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                 <  1:3512753/101‑1 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                 <  1:2096971/101‑1 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                 >  2:2690563/1‑101 (MQ=255)
                                                              cgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCa                               >  2:2673230/1‑101 (MQ=255)
                                                               gaCAGGAAGACCTGCTGCGAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCTGTCAg                              <  2:2829377/101‑1 (MQ=255)
                                                               gaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                              >  1:2036963/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                            >  2:2426994/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                            <  2:1970435/101‑1 (MQ=255)
                                                                  atgAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcgc                           >  1:1977512/3‑101 (MQ=255)
                                                                   ggAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGca                          <  1:541058/101‑1 (MQ=255)
                                                                   ggAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGca                          <  2:2851298/101‑1 (MQ=255)
                                                                      aGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatca                       >  2:2481250/1‑101 (MQ=255)
                                                                      aGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatca                       >  1:256144/1‑101 (MQ=255)
                                                                        aCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATg                     >  1:3493559/1‑101 (MQ=255)
                                                                         ccTGCGGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGc                    <  2:2984858/101‑1 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc                 <  1:1617888/101‑1 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc                 >  2:355600/1‑101 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg                >  1:3338530/1‑101 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg                >  2:3128791/1‑101 (MQ=255)
                                                                              tgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgc               >  2:3504781/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg              >  1:3161837/1‑101 (MQ=255)
                                                                                cAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCgg             >  2:1739662/1‑101 (MQ=255)
                                                                                cAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCgg             >  2:3275962/1‑101 (MQ=255)
                                                                                  agagcccccccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCa           <  1:2275985/93‑1 (MQ=255)
                                                                                  agagACCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCa           <  2:492986/101‑1 (MQ=255)
                                                                                   gagaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAt          >  2:2222949/1‑101 (MQ=255)
                                                                                   gagaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAt          <  2:78098/101‑1 (MQ=255)
                                                                                   gagaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAt          <  2:2757852/101‑1 (MQ=255)
                                                                                   gagaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAt          >  2:3233180/1‑101 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg         >  2:1559040/1‑101 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg         <  1:2960104/101‑1 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg         <  1:2774650/101‑1 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg         >  1:3003855/1‑101 (MQ=255)
                                                                                       tCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggct      <  2:3354806/101‑1 (MQ=255)
                                                                                       tCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGCGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggct      >  1:3619193/1‑101 (MQ=255)
                                                                                        cGCCCCCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctg     <  1:3948358/101‑1 (MQ=255)
                                                                                         gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg    <  1:1785194/101‑1 (MQ=255)
                                                                                         ccccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg    <  1:1492761/100‑1 (MQ=255)
                                                                                          cccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACATGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctggc   <  1:3916201/101‑1 (MQ=255)
                                                                                               |                                                                                                
CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGCC  >  NC_014034/2731705‑2731896

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG