Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	81.4	29	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (12/17):  ref base (0/0):  total (12/17)

ATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747760‑2747950                                                                                                 |                                                                                                  atgggAAACTCGCCCGACGTTTCCCCGCCCGCAGCTCACGGTTGCGGCATGACCCCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgaga                                                                                              <  2:1698458/98‑1 (MQ=21) agagaaaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgaga                                                                                              <  1:898396/93‑1 (MQ=25)  tcggAAACGCGGCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGaa                                                                                             <  2:1362957/99‑1 (MQ=25)   aGGAACCGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAg                                                                                            <  2:3426537/101‑1 (MQ=37)   aGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAg                                                                                            >  2:889843/1‑101 (MQ=255)      aaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAgcgc                                                                                         <  2:3879171/98‑1 (MQ=25)      aaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAgcgc                                                                                         <  1:1448915/98‑1 (MQ=25)        ccgcgCCCCGCGGTCCCCCGCCCGCAGCGCCCGGTTGCGGCCTGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                       <  1:3099668/100‑1 (MQ=21)          gcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgc                                                                                     <  1:1706810/101‑1 (MQ=25)           cccccctgctttCCCCGCCCGCAGCGTCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCCCAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgcc                                                                                    <  2:242858/92‑1 (MQ=18)            cccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCccgcc.g                                                                                  <  2:4106001/99‑1 (MQ=25)             ccccggtttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCATCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  2:2113541/91‑1 (MQ=21)             ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  2:3809635/100‑1 (MQ=25)              cccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.ggg                                                                                <  2:2420095/101‑2 (MQ=25)               ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCGgcc.gggc                                                                               <  1:226249/101‑3 (MQ=25)               ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggc                                                                               <  2:1972616/101‑3 (MQ=25)                cggtttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  2:3534774/94‑4 (MQ=21)                  cgTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCccc.gggccgg                                                                            >  1:3164991/1‑96 (MQ=25)                    tccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                          <  2:2514919/98‑8 (MQ=21)                     ttCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgggcg                                                                         >  1:3770226/1‑93 (MQ=25)                                             cgccaggcccGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGCCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTc                                                  <  2:3769969/93‑1 (MQ=17)                                                                  caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCtgtg                             >  2:3225047/1‑101 (MQ=14)                                                                  caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCtgtg                             >  2:2589593/1‑101 (MQ=14)                                                                                  gcccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg             >  1:2680314/1‑101 (MQ=18)                                                                                    ccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt           >  1:991505/1‑101 (MQ=18)                                                                                     cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg          >  1:2270128/1‑101 (MQ=18)                                                                                      ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg         >  2:177914/1‑101 (MQ=18)                                                                                      ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg         >  1:349115/1‑101 (MQ=18)                                                                                           cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt     >  1:81200/3‑101 (MQ=21)                                                                                              cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGACCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGACTTGGc  >  2:2129027/1‑101 (MQ=21)                                                                                                 |                                                                                                  ATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747760‑2747950 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG