Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,809,617 G→T 100% P318H (CCC→CAC)  acsA2 ← acetate‑‑CoA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,809,6170G→T100.0% 505.1 146P318H (CCC→CAC) acsA2acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (74/72):  ref base (0/0):  total (74/72)

Only 100 of 151 total aligned reads displayed.

CACCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGGGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCATAG  >  NC_014034/2809520‑2809706
                                                                                                 |                                                                                         
 acCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAcc                                                                                       <  1:3429550/101‑1 (MQ=255)
  ccGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAccc                                                                                      >  1:2480081/1‑101 (MQ=255)
  ccGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAccc                                                                                      <  1:460797/101‑1 (MQ=255)
   cGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCg                                                                                     <  1:3942673/101‑1 (MQ=255)
    gCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGt                                                                                    >  1:2706567/1‑101 (MQ=255)
        aGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAcc                                                                                <  1:846381/101‑1 (MQ=255)
        aGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAcc                                                                                <  1:2373335/101‑1 (MQ=255)
         gAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAccc                                                                               >  2:3960175/1‑101 (MQ=255)
         gAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAccc                                                                               <  1:2639279/101‑1 (MQ=255)
          aaCCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCa                                                                              >  1:2755135/1‑101 (MQ=255)
          aaCCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCa                                                                              >  1:2063713/1‑101 (MQ=255)
          aaCCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCa                                                                              <  1:2545936/101‑1 (MQ=255)
            ccGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGc                                                                            <  2:3945986/101‑1 (MQ=255)
              gCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGccc                                                                          >  1:2176825/1‑101 (MQ=255)
               ccccGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCca                                                                         >  2:3215493/1‑101 (MQ=255)
                   gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                     >  1:1692017/1‑101 (MQ=255)
                   gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                     >  1:1878015/1‑101 (MQ=255)
                   gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                     >  1:997351/1‑101 (MQ=255)
                   gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCCCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                     >  1:399050/1‑101 (MQ=255)
                    cATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcg                                                                    <  2:1207789/101‑1 (MQ=255)
                     aTCGGGCCAGGTCGGCACGGCCTCGACCATCAGCGTCGTCGCGCCGTTCGCGACGCGCCCGTAGATGGTGTAGCTCTGACCCGTCACCTAGCCCACATcgg                                                                   >  2:1466288/1‑101 (MQ=255)
                     aTCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcgg                                                                   >  2:252046/1‑101 (MQ=255)
                     aTCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcgg                                                                   >  2:2807407/1‑101 (MQ=255)
                     aTCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcgg                                                                   >  1:201692/1‑101 (MQ=255)
                     aTCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcgg                                                                   >  2:3464016/1‑101 (MQ=255)
                     aTCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcgg                                                                   >  1:103541/1‑101 (MQ=255)
                      tCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggc                                                                  <  2:3533223/101‑1 (MQ=255)
                      tCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggc                                                                  <  2:565362/101‑1 (MQ=255)
                      tCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGACCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggc                                                                  >  1:734571/1‑101 (MQ=255)
                       cGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggcg                                                                 >  1:439727/1‑101 (MQ=255)
                        gggCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggcgg                                                                >  1:394406/1‑101 (MQ=255)
                        gggCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggcgg                                                                >  2:895207/1‑101 (MQ=255)
                         ggCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGt                                                               >  2:466976/1‑101 (MQ=255)
                          gCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTg                                                              <  2:1461436/101‑1 (MQ=255)
                          gCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTg                                                              <  2:1890838/101‑1 (MQ=255)
                          gCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTg                                                              >  1:214181/1‑101 (MQ=255)
                          gCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTg                                                              <  2:3887582/101‑1 (MQ=255)
                           ccAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGc                                                             >  2:3361883/1‑101 (MQ=255)
                            cAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCa                                                            <  1:2366359/101‑1 (MQ=255)
                            cAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCa                                                            <  1:1837020/101‑1 (MQ=255)
                             aGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCAc                                                           >  2:1073834/1‑101 (MQ=255)
                              ggTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCAcc                                                          <  2:615768/101‑1 (MQ=255)
                                tCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAg                                                        <  1:3337946/101‑1 (MQ=255)
                                tCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAg                                                        <  1:3296421/101‑1 (MQ=255)
                                 cGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGa                                                       >  2:1488881/1‑101 (MQ=255)
                                 cGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGa                                                       >  2:3736150/1‑101 (MQ=255)
                                  ggCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGaa                                                      >  1:2488525/1‑101 (MQ=255)
                                  ggCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGaa                                                      <  1:3821163/101‑1 (MQ=255)
                                  ggCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGaa                                                      >  2:614855/1‑101 (MQ=255)
                                     aCGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAacac                                                   <  2:1317320/101‑1 (MQ=255)
                                     aCGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAacac                                                   >  1:3411362/1‑101 (MQ=255)
                                      cGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACg                                                  <  1:4123452/101‑1 (MQ=255)
                                       gCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTCGATGATGTAGCTGTGACCCGTCACCCAGCCCCCATCGGCGGTGCACCAGAACACgg                                                 >  2:2870395/1‑100 (MQ=255)
                                       gCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGt                                                 <  1:509626/101‑1 (MQ=255)
                                        cccTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTc                                                >  1:1623523/1‑101 (MQ=255)
                                        cccTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTc                                                <  2:4138487/101‑1 (MQ=255)
                                         ccTCGAACATCAGCGTCGTCGCGCCGTTCGCAAAGGGCCCGTAGATGATGTAGCTGTAACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCg                                               <  1:2217186/101‑1 (MQ=255)
                                             gAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGt                                           >  2:2437916/1‑101 (MQ=255)
                                             gAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGt                                           <  1:1024844/101‑1 (MQ=255)
                                              aaCATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTc                                          <  2:1310836/101‑1 (MQ=255)
                                               aCATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTcc                                         >  2:2207681/1‑101 (MQ=255)
                                                cATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCt                                        >  1:789872/1‑101 (MQ=255)
                                                cATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCt                                        >  2:3251417/1‑101 (MQ=255)
                                                cATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCt                                        >  2:2090230/1‑101 (MQ=255)
                                                 aTCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCtt                                       >  2:187007/1‑101 (MQ=255)
                                                 aTCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCtt                                       >  1:2813824/1‑101 (MQ=255)
                                                   cAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGt                                     <  2:2360845/101‑1 (MQ=37)
                                                   cAGCGTCGCCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGt                                     <  1:551760/101‑1 (MQ=255)
                                                     gCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTaa                                   >  1:2732958/1‑101 (MQ=25)
                                                     gCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTaa                                   <  1:4013520/101‑1 (MQ=21)
                                                      cgtcgtCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAAt                                  <  2:2754916/101‑1 (MQ=18)
                                                       gtcgtcGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCCCCAGAACACGTCGCCGTCCTTGTaagc                                 >  1:4100419/1‑99 (MQ=12)
                                                       gtcgtcGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATc                                 >  2:2396326/1‑101 (MQ=17)
                                                       gtcgtcGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATc                                 <  2:497794/101‑1 (MQ=17)
                                                       gtcgtcGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATc                                 <  2:1515866/101‑1 (MQ=17)
                                                       gtcgtcGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATc                                 <  1:2282513/101‑1 (MQ=17)
                                                        tcgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCa                                <  1:3059044/101‑1 (MQ=17)
                                                        tcgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCa                                <  1:2036863/101‑1 (MQ=17)
                                                        tcgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCa                                <  1:1486014/101‑1 (MQ=17)
                                                         cgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaa                               <  2:3008218/101‑1 (MQ=17)
                                                         cgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaa                               >  2:1789625/1‑101 (MQ=17)
                                                         cgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaa                               >  1:2564255/1‑101 (MQ=17)
                                                         cgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCAGTCCTTGTAATCaa                               >  2:2698890/1‑101 (MQ=12)
                                                          gtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaaa                              >  2:2302576/1‑101 (MQ=17)
                                                          gtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGCCCTTGTAATCaaa                              >  2:3981231/1‑101 (MQ=12)
                                                            cgcgCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACg                            <  1:3464268/101‑1 (MQ=17)
                                                             gcgcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgt                           >  1:2003234/1‑101 (MQ=17)
                                                             gcgcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgt                           >  2:3569689/1‑101 (MQ=17)
                                                             gcgcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgt                           >  2:1228087/1‑101 (MQ=18)
                                                              cgcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtg                          <  2:1942191/101‑1 (MQ=17)
                                                               gcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtgt                         <  2:3176739/101‑1 (MQ=17)
                                                               gcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtgt                         <  1:3606802/101‑1 (MQ=17)
                                                                 cGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTAt                       >  2:1789443/1‑101 (MQ=14)
                                                                  gTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACNNNNNGCCGTCCTTGTAATCAAACGTGTAtt                      >  1:2764844/1‑101 (MQ=2)
                                                                  gTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTAtt                      <  1:2915267/101‑1 (MQ=11)
                                                                   ttCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTg                     >  2:2553460/1‑101 (MQ=11)
                                                                        cAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCg                >  2:1832690/1‑101 (MQ=11)
                                                                                cGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATcgcg        >  1:3447242/1‑101 (MQ=11)
                                                                                 gTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCgg       >  1:158550/1‑101 (MQ=11)
                                                                                      tgatgTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCATAg  >  2:371577/1‑101 (MQ=11)
                                                                                                 |                                                                                         
CACCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGGGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCATAG  >  NC_014034/2809520‑2809706

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG