Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,925,566 A→C 100% intergenic (+350/‑85) RCAP_rcc02735 → / → RCAP_rcc02736 virulence‑associated protein E/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,925,5660A→C100.0% 931.5 245intergenic (+350/‑85)RCAP_rcc02735/RCAP_rcc02736virulence‑associated protein E/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (118/127):  ref base (0/0):  total (118/127)

Only 100 of 250 total aligned reads displayed.

AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.A.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663
                                                                                                   |                                                                                                 
aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGc                                                                                                 >  1:561167/1‑101 (MQ=255)
aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGc                                                                                                 >  1:2001994/1‑101 (MQ=255)
 ggTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCa                                                                                                >  2:1606177/1‑101 (MQ=255)
  gTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAt                                                                                               >  2:3529272/1‑101 (MQ=255)
   tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                                                              >  1:4105669/1‑101 (MQ=255)
    gggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAttt                                                                                             >  1:1873496/1‑101 (MQ=255)
       aCAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTcc                                                                                          <  1:1141930/101‑1 (MQ=255)
          aGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTg                                                                                       >  2:2623111/1‑101 (MQ=255)
            cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGAt                                                                                     >  2:3022122/1‑101 (MQ=255)
             aTTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATc                                                                                    <  1:362823/101‑1 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  <  2:1999496/101‑1 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  >  2:2331457/1‑101 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  <  1:2170220/101‑1 (MQ=255)
               tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGa                                                                                  >  2:3155245/1‑101 (MQ=255)
               cgTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGAGACG.GGGGACAGCCCACCGCGAGTTGGGACATGGATGCGGTCCGGGGCATTTTCCTTGACCgg                                                                                  >  2:3782760/2‑100 (MQ=255)
                gTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAg                                                                                 >  1:1988813/1‑101 (MQ=255)
                     gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCt                                                                            >  1:1006393/1‑101 (MQ=255)
                     gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCt                                                                            <  2:3377004/101‑1 (MQ=255)
                      aaaGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTg                                                                           >  1:2294108/1‑101 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          <  2:1935825/101‑1 (MQ=255)
                        aGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCa                                                                         >  2:128873/1‑101 (MQ=255)
                        aGGAAAATTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCa                                                                         <  2:149749/101‑1 (MQ=255)
                           aaaaTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACg                                                                      >  2:2937747/1‑101 (MQ=255)
                           aaaaTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACg                                                                      <  2:2998575/101‑1 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     >  1:580374/1‑101 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     >  2:1656362/1‑101 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     >  2:1763983/1‑101 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     <  2:2123606/101‑1 (MQ=255)
                              aTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGa                                                                   >  1:3161624/1‑101 (MQ=255)
                              aTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGa                                                                   >  1:1659877/1‑101 (MQ=255)
                              aTTTGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGa                                                                   >  2:4132237/1‑101 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 >  2:1792377/1‑101 (MQ=255)
                                 tGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTc                                                                <  2:1889010/101‑1 (MQ=255)
                                 tGACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGGGGGACATGGATGCGATCGGGCGCATTTTCCTTGATCGAGTCCCTGCAACGTGATTc                                                                >  2:2038108/1‑101 (MQ=255)
                                  gACGTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAttac                                                               >  2:1590704/1‑99 (MQ=255)
                                   aCGTCCCACCTGAGCGGTGA.AGTGGGACTTCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTccc                                                              <  2:1857393/101‑1 (MQ=255)
                                     gTCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTg                                                            >  1:2766150/1‑101 (MQ=255)
                                      tCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGc                                                           <  2:2757325/101‑1 (MQ=255)
                                      tCCCACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGc                                                           <  1:3741794/101‑1 (MQ=255)
                                       cccACTTGAGCGGTGA.AGGGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCt                                                          <  2:3094614/101‑1 (MQ=255)
                                        ccACTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTc                                                         <  1:3815266/101‑1 (MQ=255)
                                          aCTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCAt                                                       >  1:2366083/1‑101 (MQ=255)
                                           cTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATg                                                      <  1:3194544/101‑1 (MQ=255)
                                           cTTGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATg                                                      >  2:1741073/1‑101 (MQ=255)
                                            ttGAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGa                                                     >  2:852270/1‑101 (MQ=255)
                                              gAGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAcc                                                   <  2:4089115/101‑1 (MQ=255)
                                               aGCGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCa                                                  >  2:3984665/1‑101 (MQ=255)
                                                 cGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                <  1:2912335/101‑1 (MQ=255)
                                                 cGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                <  2:3670848/101‑1 (MQ=255)
                                                 cGGTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa                                                >  2:3876763/1‑101 (MQ=255)
                                                  ggTGA.AGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAg                                               <  1:836/101‑1 (MQ=255)
                                                    gtgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGc                                              >  2:112987/7‑101 (MQ=255)
                                                    gtgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGc                                              <  1:1792496/95‑1 (MQ=255)
                                                     tgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGcg                                             >  2:3141589/6‑100 (MQ=255)
                                                      taagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            <  2:544579/97‑1 (MQ=255)
                                                      gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            <  1:3529525/97‑1 (MQ=255)
                                                      gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            <  2:939853/97‑1 (MQ=255)
                                                      gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            >  2:2496443/5‑101 (MQ=255)
                                                       aagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGa                                           <  1:1720888/98‑1 (MQ=255)
                                                        agtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGaa                                          <  2:1159501/99‑1 (MQ=255)
                                                        agtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGaa                                          >  1:822698/3‑101 (MQ=255)
                                                         gtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAg                                         <  1:3722732/100‑1 (MQ=255)
                                                         gtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAg                                         <  2:1459868/100‑1 (MQ=255)
                                                          tGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAgg                                        >  2:568692/1‑101 (MQ=255)
                                                           gggACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGACGGc                                       >  2:955484/1‑101 (MQ=255)
                                                           gggACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGc                                       <  1:4025329/101‑1 (MQ=255)
                                                             gACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAACCCGAAGGccc                                     >  1:1300386/1‑101 (MQ=255)
                                                              gcAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCCTTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCa                                    <  1:1388978/100‑1 (MQ=255)
                                                               cAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCAc                                   <  2:3851516/101‑1 (MQ=255)
                                                                  cccAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGAt                                <  1:3984925/101‑1 (MQ=255)
                                                                  cccAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGAt                                >  1:3344939/1‑101 (MQ=255)
                                                                  cccAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGAt                                <  2:2256937/101‑1 (MQ=255)
                                                                   ccAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGccaagacgc                               <  2:2083252/101‑8 (MQ=255)
                                                                    cAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATcc                              >  2:2594234/1‑101 (MQ=255)
                                                                     aaaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCa                             <  1:1269051/101‑1 (MQ=255)
                                                                      aaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAg                            <  2:3614997/101‑1 (MQ=255)
                                                                      aaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAg                            >  2:2924471/1‑101 (MQ=255)
                                                                        gCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGt                          <  1:1600278/101‑1 (MQ=255)
                                                                          gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCTACGATCCAGGTGa                        >  1:3640014/1‑101 (MQ=255)
                                                                          gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        >  2:758541/1‑101 (MQ=255)
                                                                          gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        <  1:185832/101‑1 (MQ=255)
                                                                              tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    <  1:2937969/101‑1 (MQ=255)
                                                                                ggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAATGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtc                  <  2:1006302/101‑1 (MQ=255)
                                                                                gaaCAGGGATCCGG.CGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTcca                 <  1:3128037/99‑1 (MQ=255)
                                                                                   cATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatc               >  1:3877984/1‑101 (MQ=255)
                                                                                    aTGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatca              <  1:4233025/101‑1 (MQ=255)
                                                                                    aTGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatca              >  1:143514/1‑101 (MQ=255)
                                                                                    aTGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatca              <  1:2096927/101‑1 (MQ=255)
                                                                                    aTGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatca              <  1:3091998/101‑1 (MQ=255)
                                                                                     tGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTcatcat             >  2:51508/1‑101 (MQ=255)
                                                                                     tGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTcatcat             <  2:3821487/101‑1 (MQ=255)
                                                                                      ggATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATg            >  1:2600905/1‑101 (MQ=255)
                                                                                      ggATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATg            <  2:255610/101‑1 (MQ=255)
                                                                                           cGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAcccc       <  2:1380347/101‑1 (MQ=255)
                                                                                            ggTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCg      >  2:473763/1‑101 (MQ=255)
                                                                                             gTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGa     >  2:3679420/1‑101 (MQ=255)
                                                                                              tCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGaa    <  1:873711/101‑1 (MQ=255)
                                                                                              tCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGaa    <  2:1688046/101‑1 (MQ=255)
                                                                                                gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  2:392498/1‑101 (MQ=255)
                                                                                                gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  2:2432595/1‑101 (MQ=255)
                                                                                                   |                                                                                                 
AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGA.A.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG