Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,711 A→G 2.1% intergenic (+54/‑106) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7110A→G 2.1% 3.6 ‑1.6 ‑0.8237intergenic (+54/‑106)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (111/120):  total (115/122)
Fisher's exact test strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.55e-01

Only 100 of 263 total aligned reads displayed.

TGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGG  >  NC_014034/2950611‑2950811
                                                                                                     |                                                                                                     
tGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.Gaaa                                                                                                       >  1:1104855/1‑101 (MQ=255)
 gCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAac                                                                                                      <  1:3296998/101‑1 (MQ=255)
  cccGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAaca                                                                                                     <  2:509667/101‑1 (MQ=255)
  cccGGGGGCCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAaca                                                                                                     <  1:3041428/101‑1 (MQ=255)
     ggggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGc                                                                                                  >  2:3236244/1‑101 (MQ=255)
     ggggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGc                                                                                                  <  2:1132263/101‑1 (MQ=255)
     ggggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGc                                                                                                  >  1:2047676/1‑101 (MQ=255)
         ggccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCt                                                                                              <  2:1404444/100‑1 (MQ=255)
            cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTc                                                                                           <  1:2124105/101‑1 (MQ=255)
            cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTc                                                                                           <  2:372019/101‑1 (MQ=255)
             gccCTCGCCGCAATATGCGGAACTTGATTTCTGAACCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTTAGGCAAGAT.GAAACACACCCCTCTCa                                                                                          >  1:3717913/1‑101 (MQ=37)
              cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCAt                                                                                         <  2:1463052/101‑1 (MQ=255)
               ccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATc                                                                                        <  1:237820/101‑1 (MQ=255)
                cTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCt                                                                                       <  1:3588974/101‑1 (MQ=255)
                  cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTGGGGGAAAATGGAAAAACCCCCCTTTctcctt                                                                                      >  1:826215/1‑96 (MQ=25)
                  cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTg                                                                                     <  1:2528764/101‑1 (MQ=255)
                  cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTg                                                                                     >  2:220231/1‑101 (MQ=255)
                    ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGcc                                                                                   >  2:2691340/1‑101 (MQ=255)
                    ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGcc                                                                                   >  1:1367247/1‑101 (MQ=255)
                    ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACAAGCCCCTTTCATCTTGcc                                                                                   >  2:2499871/1‑101 (MQ=255)
                        aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCaa                                                                               <  1:2322670/101‑1 (MQ=255)
                         atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCaaa                                                                              <  2:2084593/101‑1 (MQ=255)
                         atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCaaa                                                                              >  1:2416020/1‑101 (MQ=255)
                          tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAat                                                                             >  1:4013557/1‑101 (MQ=255)
                            tGCGGAGCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                           <  1:3209846/101‑1 (MQ=255)
                            tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                           <  2:2907184/101‑1 (MQ=255)
                            tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                           <  2:753643/101‑1 (MQ=255)
                             gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                          <  1:2954374/101‑1 (MQ=255)
                              cGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATcc                                                                         >  2:620059/1‑101 (MQ=255)
                              cGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCACTTTCATCTTGCCCCAAATATcc                                                                         >  2:798288/1‑101 (MQ=255)
                               ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                        <  1:380291/101‑1 (MQ=255)
                               ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                        >  1:2746568/1‑101 (MQ=255)
                                gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.g                                                                      >  2:1606481/1‑101 (MQ=255)
                                gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.g                                                                      <  2:2774583/101‑1 (MQ=255)
                                 aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gg                                                                     <  2:3066487/101‑1 (MQ=255)
                                    ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCCAATATCCC.ggggg                                                                  >  2:3622401/1‑101 (MQ=255)
                                      gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAGATATCCC.GGGGGTg                                                                >  2:2024222/1‑101 (MQ=255)
                                      gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                <  2:845318/101‑1 (MQ=255)
                                      gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                <  2:1096026/101‑1 (MQ=255)
                                       aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACAGGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGa                                                               <  2:3507793/101‑1 (MQ=255)
                                       aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGAAAGAT.GAAACACGCCCCCTTCCTCTTCCCCCACACATCCC.CGGGTTgc                                                               >  1:1571062/1‑100 (MQ=21)
                                        tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                              <  2:3322644/101‑1 (MQ=255)
                                         ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                             >  1:2055426/1‑101 (MQ=255)
                                          tCTGAACCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                            <  1:4164012/101‑1 (MQ=255)
                                           cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                           <  1:1606590/101‑1 (MQ=255)
                                             gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGc                                                         <  2:3400007/101‑1 (MQ=255)
                                               agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                       >  2:22958/1‑101 (MQ=255)
                                                 caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                     >  1:3677691/1‑101 (MQ=255)
                                                  gagcGGGGGGCTCCGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                    <  1:798366/100‑1 (MQ=255)
                                                    gctgggggCTGTGCCCCCGGTCCCCCGGGATATTTGAGGCAAGTT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                  <  2:1003642/98‑1 (MQ=21)
                                                     ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                 >  1:3020743/1‑101 (MQ=255)
                                                     ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                 >  2:382528/1‑101 (MQ=255)
                                                      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                <  1:3236485/101‑1 (MQ=255)
                                                      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                >  2:2574293/1‑101 (MQ=255)
                                                       gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                               >  1:1691456/1‑101 (MQ=255)
                                                       gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                               >  2:3906159/1‑101 (MQ=255)
                                                        ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaaa                                              <  1:2798641/101‑1 (MQ=255)
                                                         gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                             <  1:4157540/101‑1 (MQ=255)
                                                         gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                             <  2:2615240/101‑1 (MQ=255)
                                                          ggCGCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCATaga                                            <  2:3585203/101‑1 (MQ=255)
                                                           gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCACATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                           >  1:1043190/1‑101 (MQ=255)
                                                              ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                        <  1:1031780/101‑1 (MQ=255)
                                                              ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                        >  2:1522663/1‑101 (MQ=255)
                                                              ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                        <  1:908362/101‑1 (MQ=255)
                                                                ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT..AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggc                                     >  1:1799896/9‑99 (MQ=25)
                                                                gCCGCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGc                                      <  2:2104001/101‑1 (MQ=255)
                                                                gCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGc                                      <  1:4066387/101‑1 (MQ=255)
                                                                gCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGCGAATTTGCCGTCAGGCAAAGAGGGGGc                                      >  2:581151/1‑101 (MQ=255)
                                                                 cccccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                     <  2:2449930/101‑1 (MQ=255)
                                                                 cccccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                     <  2:3201303/101‑1 (MQ=255)
                                                                  cgcctcaCCCCCGGGATATTTGGGGCAAGAT..AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                   >  2:1726524/7‑97 (MQ=21)
                                                                   tcgctccccccGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcg                                  >  2:199602/7‑100 (MQ=2)
                                                                   tcgctccccccGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcg                                  >  2:3637346/7‑100 (MQ=2)
                                                                   cccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGc                                   <  2:914502/101‑1 (MQ=255)
                                                                         cccccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccccc                             >  1:2211765/1‑101 (MQ=255)
                                                                          ccccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                            >  1:1306311/1‑101 (MQ=255)
                                                                          ccccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                            <  2:1845393/101‑1 (MQ=255)
                                                                          ccccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                            <  1:519135/101‑1 (MQ=255)
                                                                            ccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGc                          >  1:1634311/1‑101 (MQ=255)
                                                                             cGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcc                         >  1:1555043/1‑101 (MQ=255)
                                                                             cGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcc                         >  1:1754789/1‑101 (MQ=255)
                                                                               ggATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcccc                       >  1:1465297/1‑101 (MQ=255)
                                                                                 atatTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                     >  2:1332001/1‑101 (MQ=255)
                                                                                  tatTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                    >  1:2866071/1‑101 (MQ=255)
                                                                                   atTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAACTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                   >  2:2162734/1‑101 (MQ=255)
                                                                                   atTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                   <  1:2638319/101‑1 (MQ=255)
                                                                                     ttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                 <  2:414875/101‑1 (MQ=255)
                                                                                     ttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                 <  1:2114106/101‑1 (MQ=255)
                                                                                     ttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                 >  1:1915877/1‑101 (MQ=255)
                                                                                      tGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgag                >  1:1767250/1‑101 (MQ=255)
                                                                                       gAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg               >  1:3516674/1‑101 (MQ=255)
                                                                                        aGGCAAGAT.GAAACCCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg              <  2:3303419/101‑1 (MQ=255)
                                                                                        aGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg              <  2:1760871/101‑1 (MQ=255)
                                                                                         ggCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga             <  2:3371096/101‑1 (MQ=255)
                                                                                         ggCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga             <  2:2055288/101‑1 (MQ=255)
                                                                                            aaGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc          >  2:4080541/1‑101 (MQ=255)
                                                                                            aaGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc          >  1:2294379/1‑101 (MQ=255)
                                                                                             aGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg         >  1:3460588/1‑101 (MQ=255)
                                                                                                 taaaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa      <  1:3318350/99‑1 (MQ=255)
                                                                                                    aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg   <  1:1501285/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGG  >  NC_014034/2950611‑2950811

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG