Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,719 C→A 2.0% intergenic (+62/‑98) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7190C→A 2.0% 2.7 ‑1.6 ‑1.2241intergenic (+62/‑98)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (112/124):  total (116/125)
Fisher's exact test strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.41e-02

Only 100 of 259 total aligned reads displayed.

GTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTG  >  NC_014034/2950619‑2950819
                                                                                                     |                                                                                                     
 ggccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCt                                                                                                      <  2:1404444/100‑1 (MQ=255)
  gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCtt                                                                                                     <  1:2233585/101‑1 (MQ=255)
    cgccCTCGCCGCAGTATGCGGAACTTGATTTCTGAAGGAAGCCGGGGGCTCTGCCCCGGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCttgc                                                                                                   <  2:359002/101‑3 (MQ=255)
    cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTc                                                                                                   <  2:372019/101‑1 (MQ=255)
    cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTc                                                                                                   <  1:4059874/101‑1 (MQ=255)
    cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTc                                                                                                   <  1:2124105/101‑1 (MQ=255)
     ccccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCa                                                                                                  <  1:175623/100‑1 (MQ=255)
      cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCAt                                                                                                 <  2:1463052/101‑1 (MQ=255)
       ccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATc                                                                                                <  1:237820/101‑1 (MQ=255)
        cTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCt                                                                                               <  1:3588974/101‑1 (MQ=255)
        cTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCt                                                                                               >  2:2225915/1‑101 (MQ=255)
          cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTGGGGGAAAATGGAAAAACCCCCCTTTctcctt                                                                                              >  1:826215/1‑96 (MQ=25)
            ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGcc                                                                                           >  1:2660737/1‑101 (MQ=255)
            ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACAAGCCCCTTTCATCTTGcc                                                                                           >  2:2499871/1‑101 (MQ=255)
             cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGccc                                                                                          <  1:1465538/101‑1 (MQ=255)
              gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGcccc                                                                                         >  1:1832430/1‑101 (MQ=255)
              gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGcccc                                                                                         <  2:2791005/101‑1 (MQ=255)
                 atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                      >  2:1569739/1‑101 (MQ=255)
                 atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                      >  1:1410192/1‑101 (MQ=255)
                    tGCGGAGCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                                   <  1:3209846/101‑1 (MQ=255)
                    tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                                   <  2:753643/101‑1 (MQ=255)
                      cGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCACTTTCATCTTGCCCCAAATATcc                                                                                 >  2:798288/1‑101 (MQ=255)
                       ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                                <  1:380291/101‑1 (MQ=255)
                       ggAACTTGATTTCTGAAGCAAGCCCGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                                <  1:3745834/101‑1 (MQ=255)
                          aCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggg                                                                            <  1:1974117/101‑1 (MQ=255)
                            ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCCAATATCCC.ggggg                                                                          >  2:3622401/1‑101 (MQ=255)
                            ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                                          <  2:3379232/101‑1 (MQ=255)
                             tGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGt                                                                         <  1:3220506/101‑1 (MQ=255)
                              gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                        <  2:845318/101‑1 (MQ=255)
                               aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGAAAGAT.GAAACACGCCCCCTTCCTCTTCCCCCACACATCCC.CGGGTTgc                                                                       >  1:1571062/1‑100 (MQ=21)
                                tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                      <  2:3322644/101‑1 (MQ=255)
                                  tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                                    >  1:301116/1‑101 (MQ=255)
                                  tCTGAACCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                                    <  1:4164012/101‑1 (MQ=255)
                                   cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                                   <  1:3024708/101‑1 (MQ=255)
                                   cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                                   <  1:1606590/101‑1 (MQ=255)
                                     gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGc                                                                 <  2:3400007/101‑1 (MQ=255)
                                       agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                               >  2:22958/1‑101 (MQ=255)
                                         caagcCGGGGGCTCTGCCCCCGGACCCCCGGTATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                             <  2:2085334/101‑1 (MQ=255)
                                         caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                             >  1:3677691/1‑101 (MQ=255)
                                          gagcGGGGGGCTCCGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                            <  1:798366/100‑1 (MQ=255)
                                           agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                           <  2:376794/101‑1 (MQ=255)
                                              cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                        >  2:2574293/1‑101 (MQ=255)
                                               gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                       >  1:2771255/1‑101 (MQ=255)
                                               gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                       >  2:3906159/1‑101 (MQ=255)
                                                 gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                     <  1:1704647/101‑1 (MQ=255)
                                                 gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                     <  2:2615240/101‑1 (MQ=255)
                                                   gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCACATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                                   >  1:1043190/1‑101 (MQ=255)
                                                   gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                                   >  1:2799137/1‑101 (MQ=255)
                                                   gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                                   <  1:1547119/101‑1 (MQ=255)
                                                   gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                                   <  2:2684171/101‑1 (MQ=255)
                                                    ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgg                                                  >  1:2319292/1‑101 (MQ=255)
                                                     tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggg                                                 >  1:263262/1‑101 (MQ=255)
                                                     tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGAggg                                                 >  1:3549613/1‑101 (MQ=255)
                                                      ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                >  2:3279872/1‑101 (MQ=255)
                                                      ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                <  1:1031780/101‑1 (MQ=255)
                                                        gCCGCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGc                                              <  2:2104001/101‑1 (MQ=255)
                                                        gCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGCGAATTTGCCGTCAGGCAAAGAGGGGGc                                              >  2:581151/1‑101 (MQ=255)
                                                         cccccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                             <  2:2449930/101‑1 (MQ=255)
                                                         cccccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                             <  2:3201303/101‑1 (MQ=255)
                                                         cccccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                             <  1:3261434/101‑1 (MQ=255)
                                                          cgcctcaCCCCCGGGATATTTGGGGCAAGAT..AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                           >  2:1726524/7‑97 (MQ=21)
                                                           tcgctccccccGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcg                                          >  2:199602/7‑100 (MQ=2)
                                                           cccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGc                                           <  2:914502/101‑1 (MQ=255)
                                                            ccGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCt                                          <  2:2047538/101‑1 (MQ=255)
                                                              ctccccccGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGccc                                       >  2:503644/4‑101 (MQ=2)
                                                               gACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                                       >  2:3710208/1‑101 (MQ=255)
                                                                 cccccGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGCCCCCt                                    >  1:2048322/1‑101 (MQ=2)
                                                                  ccccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                                    <  2:1845393/101‑1 (MQ=255)
                                                                  ccccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                                    <  2:3052347/101‑1 (MQ=255)
                                                                   cccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTg                                   >  1:912589/1‑101 (MQ=255)
                                                                   cccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATACCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTg                                   <  1:2500055/101‑1 (MQ=255)
                                                                    ccGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGc                                  >  2:4046296/1‑101 (MQ=255)
                                                                        gATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                              >  1:1125413/1‑101 (MQ=255)
                                                                         atatTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                             <  1:220269/101‑1 (MQ=255)
                                                                         atatTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                             >  2:1332001/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAACTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                           >  2:2162734/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                           >  1:4154413/1‑101 (MQ=255)
                                                                            tttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                          >  2:3515479/1‑101 (MQ=255)
                                                                            tttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                          >  2:1017287/1‑101 (MQ=255)
                                                                             ttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                         <  1:1321681/101‑1 (MQ=255)
                                                                             ttGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                         <  2:414875/101‑1 (MQ=255)
                                                                              tGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgag                        >  2:1631351/1‑101 (MQ=255)
                                                                                 ggCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                     <  2:2055288/101‑1 (MQ=255)
                                                                                 ggCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                     <  2:1104761/101‑1 (MQ=255)
                                                                                 ggCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                     <  1:581215/101‑1 (MQ=255)
                                                                                  gCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                    <  1:1783586/101‑1 (MQ=255)
                                                                                    aaGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                  >  2:1549181/1‑101 (MQ=255)
                                                                                    aaGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                  <  2:3307951/101‑1 (MQ=255)
                                                                                     aGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGGTCCCCCTGCCCCGGCTGAGGGAGGCg                 >  1:1687255/1‑101 (MQ=255)
                                                                                     aGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                 >  1:3460588/1‑101 (MQ=255)
                                                                                      gAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                <  1:3418193/101‑1 (MQ=255)
                                                                                          gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGGCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc             <  1:2091647/101‑1 (MQ=255)
                                                                                           aaaCACGCCCCTTTCATCTTGCCCCAAATGTCCC.GGGGGTGAATTTGCCGTGAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaaat            <  2:660636/101‑3 (MQ=255)
                                                                                           aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt            >  2:1785090/1‑101 (MQ=255)
                                                                                            aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg           >  1:2873556/1‑101 (MQ=255)
                                                                                            aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg           >  2:2772090/1‑101 (MQ=255)
                                                                                             acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg          >  1:2410825/1‑101 (MQ=255)
                                                                                               acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa        >  2:1177052/1‑101 (MQ=255)
                                                                                                cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa       >  1:3954277/1‑101 (MQ=255)
                                                                                                 gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc      <  1:874198/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
GTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGAT.GAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTG  >  NC_014034/2950619‑2950819

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG