Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,760 C→A 4.1% intergenic (+103/‑57) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7600C→A 4.1% 14.5 ‑0.7 ‑0.1242intergenic (+103/‑57)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (7/3):  ref base (127/104):  total (134/108)
Fisher's exact test strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.96e-01

Only 100 of 262 total aligned reads displayed.

CAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGC  >  NC_014034/2950660‑2950860
                                                                                                     |                                                                                                     
caagcCGGGGGCTCTGCCCCCGGACCCCCGGTATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                                                                       <  2:2085334/101‑1 (MQ=255)
 gagcGGGGGGCTCCGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                      <  1:798366/100‑1 (MQ=255)
    ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                                                                   >  1:3020743/1‑101 (MQ=255)
    ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                                                                   >  2:382528/1‑101 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                                  <  1:3236485/101‑1 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                                  >  2:133461/1‑101 (MQ=255)
        gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.g                                                                                              <  1:4157540/101‑1 (MQ=255)
          gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.gag                                                                                            <  2:2684171/101‑1 (MQ=255)
          gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.gag                                                                                            <  1:1547119/101‑1 (MQ=255)
          gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.gag                                                                                            <  2:1825585/101‑1 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                         <  1:908362/101‑1 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                         >  2:1522663/1‑101 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                         >  2:3279872/1‑101 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                         <  1:1031780/101‑1 (MQ=255)
               gCCCCCGGGCCCCCGGGATATTTGGGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAgggggg                                                                                       >  2:127125/1‑100 (MQ=255)
               gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGCGAATTTGCCGTCAGGCAAA.GAGGGGGc                                                                                       >  2:581151/1‑101 (MQ=255)
                tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggca                                                                                     <  1:1871320/94‑4 (MQ=25)
                cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCa                                                                                      <  2:2449930/101‑1 (MQ=255)
                cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCa                                                                                      <  2:3201303/101‑1 (MQ=255)
                 cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggcag                                                                                    >  2:1726524/7‑97 (MQ=21)
                  tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGcg                                                                                   >  2:3637346/7‑100 (MQ=2)
                   ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCt                                                                                   <  2:2047538/101‑1 (MQ=255)
                   ccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCt                                                                                   >  1:280094/1‑101 (MQ=255)
                     ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTcc                                                                                 >  1:3880141/1‑101 (MQ=255)
                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                                                >  2:503644/4‑101 (MQ=2)
                       cccccaGGGATATTTGAGGCAAGATGAAGGAGCATGATTTCAGCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccccc                                                                              <  2:1687354/100‑1 (MQ=9)
                        cccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGCCCCCt                                                                             >  1:2048322/1‑101 (MQ=2)
                        cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccccc                                                                              >  1:2211765/1‑101 (MQ=255)
                        cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccccc                                                                              >  2:1306537/1‑101 (MQ=255)
                         ccccGGGATATTTGAGGCATGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                             <  1:212387/101‑1 (MQ=255)
                         ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                             >  1:1306311/1‑101 (MQ=255)
                         ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                             <  2:1845393/101‑1 (MQ=255)
                          cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATACCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTg                                                                            <  1:2500055/101‑1 (MQ=255)
                           ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGc                                                                           >  1:1634311/1‑101 (MQ=255)
                            cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcc                                                                          >  1:1754789/1‑101 (MQ=255)
                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGGAGGCCCCCCTGcccc                                                                        >  2:2579483/1‑101 (MQ=255)
                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcccc                                                                        >  2:2293478/1‑101 (MQ=255)
                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcccc                                                                        >  1:1465297/1‑101 (MQ=255)
                                 tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                                     >  1:2866071/1‑101 (MQ=255)
                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                                    >  1:3592778/1‑101 (MQ=255)
                                       aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                               <  2:1760871/101‑1 (MQ=255)
                                        ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                              <  2:1410691/101‑1 (MQ=255)
                                        ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                              <  2:3371096/101‑1 (MQ=255)
                                        ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                              <  1:581215/101‑1 (MQ=255)
                                         gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                             <  1:1783586/101‑1 (MQ=255)
                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                           <  2:3307951/101‑1 (MQ=255)
                                           aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                           >  2:4080541/1‑101 (MQ=255)
                                            aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                          >  1:3460588/1‑101 (MQ=255)
                                            aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                          >  2:2134302/1‑101 (MQ=255)
                                             gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                                         <  1:3418193/101‑1 (MQ=255)
                                                   acacGCCCCTTTCATCTTGCCCCAAATATCCG.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                                   <  2:1125319/101‑1 (MQ=255)
                                                    cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                                  <  2:1554939/101‑1 (MQ=255)
                                                      cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                                >  1:3954277/1‑101 (MQ=255)
                                                        ccctgtgcATCTGGCGGCAAATATCCC.GGGGGTGAATTTGCCGTGAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAcc                                              <  1:2346201/94‑1 (MQ=37)
                                                         cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                             <  1:862626/101‑1 (MQ=255)
                                                              tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                        >  1:1690282/1‑101 (MQ=255)
                                                              tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                        >  2:3058007/1‑101 (MQ=255)
                                                               cATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                       >  1:367406/1‑101 (MQ=255)
                                                                 tctcccccaAAATGCCCG.GGGGGTAATTTTGCCG.CAGGGAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                     <  2:2410649/96‑1 (MQ=255)
                                                                 tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                     >  2:725626/1‑101 (MQ=255)
                                                                 tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                     >  1:2779616/1‑101 (MQ=255)
                                                                  cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                    <  1:3145884/101‑1 (MQ=255)
                                                                  cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                    >  1:2654984/1‑101 (MQ=255)
                                                                   ttGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg                                   >  1:2581123/1‑101 (MQ=255)
                                                                    tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                                  <  1:2024360/101‑1 (MQ=255)
                                                                    tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                                  <  1:1569848/101‑1 (MQ=255)
                                                                       cccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                               <  2:301071/101‑1 (MQ=255)
                                                                        ccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGa                              <  2:640980/101‑1 (MQ=255)
                                                                          aaaTCTCCC.GGTGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                            <  2:4126691/101‑1 (MQ=255)
                                                                          aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                            >  1:2532418/1‑101 (MQ=255)
                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           <  1:22972/101‑1 (MQ=255)
                                                                              atCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACATTGAAGACCCGCCTGATCGAag                        <  1:1721307/101‑1 (MQ=255)
                                                                               tCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga                       <  1:2729469/101‑1 (MQ=255)
                                                                               tCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga                       >  1:3209545/1‑101 (MQ=255)
                                                                                ccc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                      <  2:3686040/101‑1 (MQ=255)
                                                                                ccc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                      >  1:1083363/1‑101 (MQ=255)
                                                                                ccc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                      >  2:3742002/1‑101 (MQ=255)
                                                                                ccc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                      <  2:4013297/101‑1 (MQ=255)
                                                                                  c.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc                    >  1:984221/1‑101 (MQ=255)
                                                                                      ggggtaaTTTGCCGTCAGCCAAA.GAGGGGGCTGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAGCCTTGAAGACCCGCCTGATCGAAGAGGCCcg                 <  1:3795648/96‑1 (MQ=255)
                                                                                        gTGAATTTCCGGTCGGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcg               <  2:2569192/101‑1 (MQ=255)
                                                                                         ttaaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgc              <  2:4160720/99‑1 (MQ=255)
                                                                                         tGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGGGGCGGAACTGGAAACCTTGAAGCCCCGCCTGATCGAAGAGGCCcgcgc              >  2:2765495/1‑101 (MQ=255)
                                                                                           aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa            >  2:1168878/1‑101 (MQ=255)
                                                                                           aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa            <  1:4118382/101‑1 (MQ=255)
                                                                                           aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa            <  1:2574484/101‑1 (MQ=255)
                                                                                           aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGACCGCGCGa            >  2:1012811/1‑101 (MQ=255)
                                                                                            aTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt           >  2:775426/1‑101 (MQ=255)
                                                                                             tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc          <  2:94837/101‑1 (MQ=255)
                                                                                             tttGCCGGCAGGAAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc          <  1:4081334/101‑1 (MQ=255)
                                                                                              ttGGCGGCAGGGAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGACGCCCGCGCGATc          <  1:2293646/101‑1 (MQ=255)
                                                                                              ttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg         >  2:1994755/1‑101 (MQ=255)
                                                                                                  cGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt     >  2:2853206/1‑101 (MQ=255)
                                                                                                  cGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt     <  2:263217/101‑1 (MQ=255)
                                                                                                  cGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGCAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt     >  2:2160348/1‑101 (MQ=255)
                                                                                                   cccAGGCAAA.GAGGGGGCAGCGCCCCCTGCCCCAGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCttt    <  2:3530291/99‑1 (MQ=255)
                                                                                                    tCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg   >  1:1348041/1‑101 (MQ=255)
                                                                                                     cAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGGGATCGGCtttag  >  1:3530544/1‑99 (MQ=255)
                                                                                                     cAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  >  2:1018235/1‑101 (MQ=255)
                                                                                                     cAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  <  1:2104024/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGC  >  NC_014034/2950660‑2950860

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG