Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,499 A→G 100% E123E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,4990A→G100.0% 864.6 225E123E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (110/115):  ref base (0/0):  total (110/115)

Only 100 of 228 total aligned reads displayed.

GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596
                                                                                                  |                                                                                                 
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgc                                                                                                 >  2:2116815/1‑101 (MQ=255)
 aCCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcg                                                                                                <  1:1031283/101‑1 (MQ=255)
 aCCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcg                                                                                                <  2:470735/101‑1 (MQ=255)
  ccGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcgc                                                                                               <  2:2604757/101‑1 (MQ=255)
  ccGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcgc                                                                                               >  2:249248/1‑101 (MQ=255)
  ccGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcgc                                                                                               >  1:1638826/1‑101 (MQ=255)
    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             >  2:3422535/1‑101 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            <  1:1318495/101‑1 (MQ=255)
       cgcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTg                                                                                          <  2:1787118/101‑1 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         <  2:2581565/101‑1 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         >  2:3337198/1‑101 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         <  1:4093299/101‑1 (MQ=255)
         ctggTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        <  2:1924285/99‑1 (MQ=255)
         cAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        >  2:2129634/1‑101 (MQ=255)
         cAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        <  2:1090581/101‑1 (MQ=255)
          aGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACg                                                                                       >  2:2934215/1‑101 (MQ=255)
          aGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACg                                                                                       <  1:1008623/101‑1 (MQ=255)
           ggTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAcag                                                                                      >  1:1924411/1‑99 (MQ=255)
           ggTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACgg                                                                                      >  1:1762075/1‑101 (MQ=255)
            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  2:314840/1‑101 (MQ=255)
             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGtt                                                                                    >  2:2721692/1‑101 (MQ=255)
                cgcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTg                                                                                 >  2:2787105/1‑101 (MQ=255)
                 gcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTgg                                                                                >  2:2982268/1‑101 (MQ=255)
                 gcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTgg                                                                                <  1:2930647/101‑1 (MQ=255)
                   ggTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGc                                                                              >  1:1350217/1‑101 (MQ=255)
                    gTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGcc                                                                             <  1:149408/101‑1 (MQ=255)
                    gTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCGCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGcc                                                                             <  1:2639071/101‑1 (MQ=255)
                     tttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCg                                                                            <  1:665068/101‑1 (MQ=255)
                     tttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCg                                                                            <  1:4065524/101‑1 (MQ=255)
                        cGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCaa                                                                         <  1:1175011/101‑1 (MQ=255)
                          cACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAc                                                                       >  2:148474/1‑101 (MQ=255)
                             cAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCgg                                                                    >  2:4195984/1‑101 (MQ=255)
                             cAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCgg                                                                    >  2:79891/1‑101 (MQ=255)
                               aCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGcc                                                                  <  1:891440/101‑1 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  1:324605/1‑101 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  1:3143205/1‑101 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               <  2:2757234/101‑2 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               >  1:2560071/1‑100 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               <  1:960472/101‑2 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               <  1:2995679/101‑2 (MQ=255)
                                    tCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGt                                                             >  2:540303/1‑101 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  2:4079929/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  1:2618332/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  2:1036613/101‑1 (MQ=255)
                                        tGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGaa                                                         <  1:736850/101‑1 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     <  2:2508787/101‑1 (MQ=255)
                                             ttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                    <  1:2122228/101‑1 (MQ=255)
                                                  gcagTTCAACCTCTGGAACCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                               <  2:3704119/101‑1 (MQ=255)
                                                   cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                              >  1:3325864/1‑101 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             >  1:2490190/1‑101 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             >  2:678496/1‑101 (MQ=255)
                                                     gTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                            <  1:4196246/101‑1 (MQ=255)
                                                      ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAg                                           <  2:2916900/101‑1 (MQ=255)
                                                       tCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGc                                          <  1:2721898/101‑1 (MQ=255)
                                                       tCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGc                                          <  1:1694305/101‑1 (MQ=255)
                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         <  2:4156585/101‑1 (MQ=255)
                                                         aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                        >  2:2053426/1‑101 (MQ=255)
                                                          aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                       <  2:1287448/101‑1 (MQ=255)
                                                           ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                      <  2:2812353/101‑1 (MQ=255)
                                                           ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                      <  2:3960946/101‑1 (MQ=255)
                                                            ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                     >  1:1092387/1‑101 (MQ=255)
                                                            ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                     >  1:152224/1‑101 (MQ=255)
                                                             tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                    <  1:3421447/101‑1 (MQ=255)
                                                             tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                    <  2:1241049/101‑1 (MQ=255)
                                                              ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                   <  1:1170624/101‑1 (MQ=255)
                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                  >  2:1417813/1‑101 (MQ=255)
                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGTCTTCTGGCCa                                <  2:897033/101‑1 (MQ=255)
                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                <  2:630389/101‑1 (MQ=255)
                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                <  1:516376/101‑1 (MQ=255)
                                                                  aGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCaa                               >  2:599295/1‑101 (MQ=255)
                                                                  aGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCaa                               >  2:3837335/1‑101 (MQ=255)
                                                                         tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGc                        <  1:2308384/101‑1 (MQ=255)
                                                                         tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGc                        >  2:645401/1‑101 (MQ=255)
                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  1:434645/1‑101 (MQ=255)
                                                                           aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                      >  1:566424/1‑101 (MQ=255)
                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     >  1:2648430/1‑101 (MQ=255)
                                                                             gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                    >  1:198989/1‑101 (MQ=255)
                                                                               tCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGACGGCATCGGc                  >  2:1760015/1‑101 (MQ=255)
                                                                                ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                 <  2:2559880/101‑1 (MQ=255)
                                                                                ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                 <  1:1417910/101‑1 (MQ=255)
                                                                                ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGAATCGGCg                 >  1:503577/1‑101 (MQ=255)
                                                                                 cTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGa                <  1:3709839/101‑1 (MQ=255)
                                                                                 cTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCAGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGa                >  2:3662801/1‑101 (MQ=255)
                                                                                  tGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGAc               >  1:3802770/1‑101 (MQ=255)
                                                                                   gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa              <  2:163880/101‑1 (MQ=255)
                                                                                   gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa              <  2:241028/101‑1 (MQ=255)
                                                                                    cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa             <  2:3495419/101‑1 (MQ=255)
                                                                                    cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa             <  1:2748760/101‑1 (MQ=255)
                                                                                     aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            >  1:1051898/1‑100 (MQ=255)
                                                                                      aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaagg           >  1:3988483/1‑99 (MQ=255)
                                                                                      aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaagg           <  1:3664061/101‑3 (MQ=255)
                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        >  2:836998/1‑101 (MQ=255)
                                                                                          gTCATGGAGGCGGCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa       >  1:2287403/1‑101 (MQ=255)
                                                                                           tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc      <  1:2787324/101‑1 (MQ=255)
                                                                                            cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg     >  1:2277161/1‑101 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   >  1:3075652/1‑101 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   <  2:3919921/101‑1 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   <  2:3609377/101‑1 (MQ=255)
                                                                                               ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt  >  1:1853284/1‑101 (MQ=255)
                                                                                               ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt  <  1:3544471/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG