Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,535 A→G 100% E135E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5350A→G100.0% 822.8 218E135E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (104/114):  ref base (0/0):  total (104/114)

Only 100 of 225 total aligned reads displayed.

TCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCG  >  NC_014034/2960437‑2960632
                                                                                                  |                                                                                                 
 cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                >  1:2642553/1‑101 (MQ=255)
 cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                <  1:2618332/101‑1 (MQ=255)
     ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                                                            <  2:1761956/101‑1 (MQ=255)
     ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                                                            >  1:2056538/1‑101 (MQ=255)
      gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                                                           <  2:584835/101‑1 (MQ=255)
      gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACAGGCCGAGGTCGAAcc                                                                                           <  1:4119288/101‑1 (MQ=255)
       tgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCg                                                                                          >  2:3185774/1‑101 (MQ=255)
         ttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                                                        <  1:2122228/101‑1 (MQ=255)
          tCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTc                                                                                       <  1:803409/101‑1 (MQ=255)
            cagcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGc                                                                                     >  1:3495671/1‑101 (MQ=255)
              gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   <  1:2762497/101‑1 (MQ=255)
              gcagTTCAACCTCTGGAACCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   <  2:3704119/101‑1 (MQ=255)
               cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                                                                  >  2:3978418/1‑101 (MQ=255)
                agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                                                                 >  2:678496/1‑101 (MQ=255)
                agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                                                                 >  1:2490190/1‑101 (MQ=255)
                agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                                                                 >  2:454069/1‑101 (MQ=255)
                 gTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                                                                <  1:188474/101‑1 (MQ=255)
                 gTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                                                                <  1:4196246/101‑1 (MQ=255)
                   tCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGc                                                                              <  2:2542306/101‑1 (MQ=255)
                    cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                                                             >  2:806938/1‑101 (MQ=255)
                    cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                                                             <  2:4156585/101‑1 (MQ=255)
                     aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                                                            <  1:1342740/101‑1 (MQ=255)
                     aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                                                            >  2:2053426/1‑101 (MQ=255)
                      aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                                                           <  2:1287448/101‑1 (MQ=255)
                      aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                                                           <  1:1759816/101‑1 (MQ=255)
                       ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                                                          <  2:3960946/101‑1 (MQ=255)
                        ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                                                         >  1:152224/1‑101 (MQ=255)
                        ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                                                         <  2:1943770/101‑1 (MQ=255)
                        ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                                                         >  1:1092387/1‑101 (MQ=255)
                         tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                                                        <  1:3421447/101‑1 (MQ=255)
                          ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                                                       >  2:3858144/1‑101 (MQ=255)
                           tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGTGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                                                      >  1:3843450/1‑101 (MQ=255)
                           tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                                                      >  2:3544218/1‑101 (MQ=255)
                           tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGc                                                                      >  1:163918/1‑101 (MQ=255)
                             gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                                                    <  1:516376/101‑1 (MQ=255)
                             gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                                                    <  1:1145803/101‑1 (MQ=255)
                                ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                                                                 >  1:326403/1‑101 (MQ=255)
                                ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                                                                 >  1:2142353/1‑101 (MQ=255)
                                ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                                                                 <  1:1024125/101‑1 (MQ=255)
                                 cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGt                                                                <  2:744736/101‑1 (MQ=255)
                                    aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                                                             <  2:2887293/101‑1 (MQ=255)
                                    aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                                                             >  1:2434742/1‑101 (MQ=255)
                                    aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                                                             <  1:2579526/101‑1 (MQ=255)
                                     tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGc                                                            >  2:645401/1‑101 (MQ=255)
                                      gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                                                           >  1:3526057/1‑101 (MQ=255)
                                       gcGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                                                          <  1:3024884/100‑1 (MQ=255)
                                       aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                                                          <  1:2690211/101‑1 (MQ=255)
                                       aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                                                          >  1:566424/1‑101 (MQ=255)
                                        cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAATCGCTCGCCCGCCTCCTTCTGCCCAAGGCCCGCATc                                                         >  1:478100/1‑101 (MQ=255)
                                         gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                                                        >  1:198989/1‑101 (MQ=255)
                                            ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                                                     <  1:1417910/101‑1 (MQ=255)
                                            ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                                                     <  2:277608/101‑1 (MQ=255)
                                            ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                                                     >  2:91655/1‑101 (MQ=255)
                                             cTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGa                                                    <  1:3709839/101‑1 (MQ=255)
                                               gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  >  2:2598371/1‑101 (MQ=255)
                                               gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  <  2:241028/101‑1 (MQ=255)
                                               gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  <  2:163880/101‑1 (MQ=255)
                                                cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa                                                 >  2:605812/1‑101 (MQ=255)
                                                cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa                                                 >  1:3141234/1‑101 (MQ=255)
                                                 aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                >  1:1051898/1‑100 (MQ=255)
                                                  aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaagg                                               <  2:3651702/101‑3 (MQ=255)
                                                      gTCATGGAGGCGGCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                           >  1:2287403/1‑101 (MQ=255)
                                                      gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                           <  1:917811/101‑1 (MQ=255)
                                                        cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                         >  2:895371/1‑101 (MQ=255)
                                                        cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                         <  1:2787349/101‑1 (MQ=255)
                                                         aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                        >  1:322423/1‑101 (MQ=255)
                                                          tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                       <  2:3919921/101‑1 (MQ=255)
                                                          tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                       <  2:3609377/101‑1 (MQ=255)
                                                           ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCTGCCTAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                      <  1:3622135/101‑1 (MQ=255)
                                                           ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                      >  1:1851602/1‑101 (MQ=255)
                                                           ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                      >  1:509485/1‑101 (MQ=255)
                                                                cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                 <  2:2490006/101‑1 (MQ=255)
                                                                cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                 >  1:391945/1‑101 (MQ=255)
                                                                cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                 >  1:205219/1‑101 (MQ=255)
                                                                 gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                >  1:2216450/1‑101 (MQ=255)
                                                                 gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                >  2:3158462/1‑101 (MQ=255)
                                                                     gTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCa                            <  1:2350027/101‑1 (MQ=255)
                                                                       gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          >  2:2947378/1‑101 (MQ=255)
                                                                       gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          >  2:3375693/1‑101 (MQ=255)
                                                                          ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                       <  1:249834/101‑1 (MQ=255)
                                                                          ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                       >  1:4050400/1‑101 (MQ=255)
                                                                           gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                      <  2:2128082/101‑1 (MQ=255)
                                                                           gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                      <  1:374532/101‑1 (MQ=255)
                                                                             tCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCgg                    <  1:1688366/101‑1 (MQ=255)
                                                                               tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                  >  2:3041246/1‑101 (MQ=255)
                                                                               tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                  <  2:1512960/101‑1 (MQ=255)
                                                                                gggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcg                 >  2:510209/1‑101 (MQ=255)
                                                                                 ggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcgg                >  2:1217022/1‑101 (MQ=255)
                                                                                    cGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca             >  1:3208227/1‑101 (MQ=255)
                                                                                     gCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag            <  2:2521267/101‑1 (MQ=255)
                                                                                      cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc           <  2:2142208/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGACCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  2:1470350/101‑1 (MQ=255)
                                                                                       aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          <  1:1250837/101‑1 (MQ=255)
                                                                                        aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGACGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag         >  1:4028492/1‑101 (MQ=255)
                                                                                         aCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCagcagc        <  2:4138218/101‑1 (MQ=255)
                                                                                         aCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCagcagc        <  1:69753/101‑1 (MQ=255)
                                                                                           cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa      <  2:2927917/101‑1 (MQ=255)
                                                                                            ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCCAc     >  2:2851109/1‑101 (MQ=255)
                                                                                            ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc     >  1:738175/1‑101 (MQ=255)
                                                                                            ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc     <  1:3287463/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCG  >  NC_014034/2960437‑2960632

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG