Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,586 A→G 100% K152K (AAA→AAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5860A→G100.0% 697.3 187K152K (AAA→AAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (100/86):  ref base (1/0):  total (101/86)

Only 100 of 192 total aligned reads displayed.

ATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGAC  >  NC_014034/2960489‑2960682
                                                                                                 |                                                                                                
 tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                              >  2:386014/1‑101 (MQ=255)
 tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                              >  2:836998/1‑101 (MQ=255)
   tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                            <  1:2787324/101‑1 (MQ=255)
    cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                                                                           >  1:2277161/1‑101 (MQ=255)
    cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                                                                           >  1:1048481/1‑101 (MQ=255)
     aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                          >  1:108877/1‑101 (MQ=255)
      tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                         >  2:3295313/1‑101 (MQ=255)
      tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                         >  1:2528257/1‑101 (MQ=255)
      tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                         <  2:3609377/101‑1 (MQ=255)
       ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                                                                        >  1:1851602/1‑101 (MQ=255)
       ccgatctccGGTGGCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                                                                        <  1:2082718/94‑1 (MQ=255)
        gaggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTg                                                                                       <  2:4181680/98‑1 (MQ=255)
            cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                   >  1:391945/1‑101 (MQ=255)
            cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                   <  2:2490006/101‑1 (MQ=255)
             gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                                                                  >  2:3158462/1‑101 (MQ=255)
             gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                                                                  >  1:3233932/1‑101 (MQ=255)
             gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                                                                  <  2:1632715/101‑1 (MQ=255)
              ccGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgc                                                                                 >  2:2464287/1‑101 (MQ=255)
                ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGACAAGGTCGGCATCGGCGAC.AAGGCGACGCCTGGCCCGCGCa                                                                              >  1:512362/1‑101 (MQ=255)
                 gTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCa                                                                              <  1:2362091/101‑1 (MQ=255)
                 gTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCa                                                                              >  2:69738/1‑101 (MQ=255)
                   gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                            >  2:2947378/1‑101 (MQ=255)
                   gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                            >  1:4042707/1‑101 (MQ=255)
                   gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                            <  1:2615510/101‑1 (MQ=255)
                    aCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                                                                           >  2:3970440/1‑101 (MQ=255)
                      ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                                                                         <  1:895456/101‑1 (MQ=255)
                       gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                        <  1:374532/101‑1 (MQ=255)
                        ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                       <  1:314885/101‑1 (MQ=255)
                         tCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCgg                                                                      >  1:3513577/1‑101 (MQ=255)
                         tCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCgg                                                                      <  1:1688366/101‑1 (MQ=255)
                          cTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCggg                                                                     >  1:3216338/1‑101 (MQ=255)
                           tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                    >  2:3546413/1‑101 (MQ=255)
                           tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                    >  2:3041246/1‑101 (MQ=255)
                           tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                    <  1:2053564/101‑1 (MQ=255)
                            gggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcg                                                                   >  2:510209/1‑101 (MQ=255)
                             ggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcgg                                                                  >  1:2146855/1‑101 (MQ=255)
                               ccGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGcc                                                                <  1:2116960/101‑1 (MQ=255)
                                cccAAGCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca                                                               <  2:4149640/99‑1 (MQ=255)
                                cGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca                                                               >  1:1375269/1‑101 (MQ=255)
                                 gCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag                                                              <  2:2521267/101‑1 (MQ=255)
                                  cAACCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc                                                             <  1:540367/101‑1 (MQ=255)
                                  cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc                                                             <  2:2142208/101‑1 (MQ=255)
                                  cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc                                                             >  2:3172998/1‑101 (MQ=255)
                                   aaaCCGGCCGAGGTCGACCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                            <  2:1470350/101‑1 (MQ=255)
                                    aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGACGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag                                                           >  1:4028492/1‑101 (MQ=255)
                                     aCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCagcagc                                                          <  1:69753/101‑1 (MQ=255)
                                     aCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCagcagc                                                          >  1:755685/1‑101 (MQ=255)
                                        ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCCAc                                                       >  2:2851109/1‑101 (MQ=255)
                                        ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                       <  1:1643112/101‑1 (MQ=255)
                                        ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                       <  1:3287463/101‑1 (MQ=255)
                                          ccGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgc                                                     >  2:952559/1‑101 (MQ=255)
                                            gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                   >  2:741235/4‑101 (MQ=255)
                                            gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                   >  1:718141/4‑101 (MQ=255)
                                             aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                  <  1:2304994/99‑1 (MQ=255)
                                               gTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccgc                                                >  1:3020660/1‑101 (MQ=255)
                                                 ctagccGCTCCCCCGCAGCCTTCGGGACAAGGTCTGGATCGTCGACAAGGCCGACGCCTGACCCGCGCAGCTCTCGGGCGGCCCGCAGCAACGCGCCGCCa                                              <  2:2107030/97‑1 (MQ=255)
                                                 cGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCa                                              >  1:947955/1‑101 (MQ=255)
                                                  gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                             >  1:1236821/1‑101 (MQ=255)
                                                  gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                             >  2:714814/1‑101 (MQ=255)
                                                  gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                             >  2:122538/1‑101 (MQ=255)
                                                   aaCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATc                                            <  2:566360/101‑1 (MQ=255)
                                                   aaCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATc                                            >  2:2875150/1‑101 (MQ=255)
                                                     ccGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGc                                          >  1:854900/1‑101 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                        >  1:1543064/1‑101 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGACAAGGTCTGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                        >  1:2494564/1‑101 (MQ=255)
                                                         tCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgc                                      >  1:473185/1‑101 (MQ=255)
                                                          cGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgcg                                     <  1:645478/101‑1 (MQ=255)
                                                             ctgCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCgcgcgc                                  <  2:2123198/99‑1 (MQ=255)
                                                             ccGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCgcgcgc                                  <  2:2567086/101‑1 (MQ=255)
                                                               gCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCNCCATCGCCCGCGCGctc                                >  2:1174711/1‑101 (MQ=255)
                                                               gCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctc                                >  2:1053927/1‑101 (MQ=255)
                                                               gCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctc                                <  2:1093825/101‑1 (MQ=255)
                                                               gCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctc                                <  2:2622883/101‑1 (MQ=255)
                                                               gCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctc                                >  1:2027774/1‑101 (MQ=255)
                                                                cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                               >  2:2663945/1‑101 (MQ=255)
                                                                cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                               <  1:3422780/101‑1 (MQ=255)
                                                                cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                               >  2:346506/1‑101 (MQ=255)
                                                                cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCCCCGACGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCCctct                               >  2:2041037/1‑101 (MQ=255)
                                                                 aGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtg                              <  2:407638/101‑1 (MQ=255)
                                                                   ccTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgca                            <  1:1986739/101‑1 (MQ=255)
                                                                   ccTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgca                            >  2:3033015/1‑101 (MQ=255)
                                                                   ccTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgca                            >  2:3821229/1‑101 (MQ=255)
                                                                    cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                           >  2:1863032/1‑101 (MQ=255)
                                                                    cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                           <  2:2287235/101‑1 (MQ=255)
                                                                      tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                         <  1:1760134/101‑1 (MQ=255)
                                                                      tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                         <  2:3233693/101‑1 (MQ=255)
                                                                       cTGGCCAAGGTCGGCATCGGCGACAAGGCCGCCGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                        >  1:3810457/1‑101 (MQ=255)
                                                                       cTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                        <  1:2129779/101‑1 (MQ=255)
                                                                        tGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAg                       >  1:2843696/1‑101 (MQ=255)
                                                                          gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                     >  1:1302486/1‑101 (MQ=255)
                                                                          gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                     >  1:2492291/1‑101 (MQ=255)
                                                                             aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                  >  1:2645227/1‑101 (MQ=255)
                                                                             aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                  <  1:2769775/101‑1 (MQ=255)
                                                                               ggTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAgg                >  1:3189546/1‑101 (MQ=255)
                                                                                  cGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGgcgc             >  1:600618/1‑101 (MQ=255)
                                                                                      aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt         <  2:1302390/101‑1 (MQ=255)
                                                                                      aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt         <  2:3628457/101‑1 (MQ=255)
                                                                                            gACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcga   <  2:3725120/101‑1 (MQ=255)
                                                                                             aCAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgac  >  1:3585962/1‑101 (MQ=255)
                                                                                             aCAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgac  >  2:3122741/1‑101 (MQ=255)
                                                                                                 |                                                                                                
ATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGAC  >  NC_014034/2960489‑2960682

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG