Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,041 G→C 14.6% intergenic (+87/+89) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0410G→C14.6% 67.8 ‑0.4 ‑0.1192intergenic (+87/+89)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (16/12):  ref base (85/79):  total (101/91)
Fisher's exact test strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.66e-01

Only 100 of 213 total aligned reads displayed.

CGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTT  >  NC_014034/3003941‑3004140
                                                                                                      |                                                                                                    
 gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCgg                                                                                                     <  1:1529295/101‑1 (MQ=255)
 gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTAAACCCCCGGGATATTTGGACCAAGGTGAAAGAAG.A.TCCCgg                                                                                                     >  2:3360997/1‑101 (MQ=255)
    cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGttt                                                                                                  <  1:1173748/101‑1 (MQ=255)
    cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGttt                                                                                                  <  1:442173/101‑1 (MQ=255)
     gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTg                                                                                                 <  1:709282/101‑1 (MQ=255)
      agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGc                                                                                                <  2:3387834/101‑1 (MQ=255)
      agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGc                                                                                                >  1:3809076/1‑101 (MQ=255)
       gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                               <  1:3147018/101‑1 (MQ=255)
       gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                               <  1:1460017/101‑1 (MQ=255)
        aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGTAAGCAG.A.TCCCGGTTTGCCt                                                                                              <  2:2028812/101‑1 (MQ=255)
        aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCt                                                                                              <  2:1909938/101‑1 (MQ=255)
          cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAACAG.A.TCCCGGGTTGCCttt                                                                                            >  2:2622012/1‑101 (MQ=255)
              gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCAcc                                                                                        >  1:1578434/1‑101 (MQ=255)
               ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCt                                                                                       <  2:3118036/101‑1 (MQ=255)
                gCAAGGCGGGGGGCGCTTCCCCCCTCTTGGCCTGACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                      <  1:3512317/101‑1 (MQ=255)
                gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCCCCtt                                                                                      >  2:4022869/1‑101 (MQ=255)
                gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                      <  2:1582462/101‑1 (MQ=255)
                 cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                     >  2:30909/1‑101 (MQ=255)
                 cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                     <  1:969716/101‑1 (MQ=255)
                  aaGGCGGGGGGGGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGCGATATTGGGACCAAGGGGAAAGCAG.A.TCCCGGCTTGCCTTTCACCttcg                                                                                    >  1:2471112/1‑99 (MQ=38)
                  aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTgg                                                                                    >  2:3272129/1‑101 (MQ=255)
                  aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTgg                                                                                    <  1:3668326/101‑1 (MQ=255)
                    ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGgcg                                                                                  <  1:3945212/101‑1 (MQ=255)
                    ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGTATTTGGACCAAGGGGGAAGCAG.A.TCCCGGGTTGGCTTTCACCTTGgcg                                                                                  >  1:869379/1‑101 (MQ=37)
                       ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aa                                                                              >  2:2365582/1‑101 (MQ=255)
                       ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGTATTTTGGCCAAAGGGAAAACAG.A.TCCCCGGTTGGCTTTCACCCTgggccca                                                                               >  2:886641/1‑96 (MQ=16)
                        gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aaa                                                                             <  1:3284533/101‑1 (MQ=255)
                             cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATcc                                                                        <  2:2896007/101‑1 (MQ=255)
                             cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATcc                                                                        >  2:2708388/1‑101 (MQ=255)
                               cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCg                                                                      <  1:3074594/101‑1 (MQ=255)
                                 gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggg                                                                    >  2:3446957/1‑101 (MQ=255)
                                  ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgggg                                                                   <  2:2275491/101‑1 (MQ=255)
                                   cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggggg                                                                  >  2:3033016/1‑101 (MQ=255)
                                     cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGa                                                                <  1:3033241/101‑1 (MQ=255)
                                          ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGTTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTccc                                                           <  2:982978/101‑1 (MQ=255)
                                          gatctcTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTccc                                                           <  1:1942635/96‑1 (MQ=255)
                                           tGGCCGGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCcg                                                          <  1:3724420/101‑1 (MQ=255)
                                            ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCcgc                                                         >  1:3983479/1‑101 (MQ=255)
                                             gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCcgcg                                                        >  2:4172714/1‑101 (MQ=255)
                                             gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCcgcg                                                        <  2:1160167/101‑1 (MQ=255)
                                               cTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCCCCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCggg                                                      <  1:2128812/101‑1 (MQ=255)
                                               cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTcccagatc                                                      >  2:1942509/1‑96 (MQ=255)
                                                tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCNGGGGGAGTCCCGCgggg                                                     >  2:930217/1‑101 (MQ=255)
                                                 gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGa                                                    >  1:1265969/1‑101 (MQ=255)
                                                 gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGa                                                    >  2:2775451/1‑101 (MQ=255)
                                                  gcGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                   <  2:1083159/100‑1 (MQ=255)
                                                  aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                   <  1:164420/101‑1 (MQ=255)
                                                  aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                   <  1:1068303/101‑1 (MQ=255)
                                                  aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                   <  1:2988739/101‑1 (MQ=255)
                                                   cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACg                                                  >  1:4175125/1‑101 (MQ=255)
                                                      ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGCGTCCCGCGGGGACgggg                                               >  2:2498185/1‑101 (MQ=255)
                                                       gttccccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGGTTCCCCCGGGG.Cgggggg                                             >  2:1217614/8‑101 (MQ=18)
                                                       cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                              >  1:688360/1‑101 (MQ=255)
                                                        aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAAGATCCCGGGGGGAGTCCCGCGGGGACggggcg                                             >  1:1011810/1‑99 (MQ=255)
                                                         aTTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACggggggg                                            >  1:3573604/1‑100 (MQ=255)
                                                         aTTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                            >  1:1785884/1‑101 (MQ=255)
                                                            ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                         >  1:1692523/3‑100 (MQ=18)
                                                            ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                         <  2:3829516/99‑2 (MQ=18)
                                                              ccccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgc                                       >  2:2544382/1‑101 (MQ=255)
                                                               cccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                      <  2:1141310/101‑1 (MQ=18)
                                                                ccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCGCGATTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                     <  1:3619766/101‑1 (MQ=255)
                                                                 cccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                    <  1:2728365/101‑1 (MQ=18)
                                                                  ccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                   <  2:1717502/101‑1 (MQ=17)
                                                                   cGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                  >  1:2891537/1‑101 (MQ=18)
                                                                   cGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                  >  2:2869701/1‑101 (MQ=18)
                                                                     ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                >  2:1014468/1‑99 (MQ=18)
                                                                      gATTTTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                               <  1:3595515/101‑1 (MQ=255)
                                                                      gATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                               >  2:3618145/1‑101 (MQ=255)
                                                                       atatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                              >  2:791856/1‑97 (MQ=18)
                                                                       atatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                              >  2:1573134/1‑97 (MQ=17)
                                                                       atatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctag                              >  1:3828987/1‑97 (MQ=18)
                                                                        tatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                             >  2:509458/1‑96 (MQ=18)
                                                                        tatTTGGCCCAAGGGGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                             <  1:802885/101‑6 (MQ=17)
                                                                        tatTTGGACCAAGGTGAAAGCAG.A.ACCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGCGTCCCGCGGGGACGGGGGGCACAgcccccctgccc                             >  1:3425444/1‑101 (MQ=255)
                                                                         atTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                            <  2:1733232/101‑7 (MQ=17)
                                                                         atTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                            <  2:3860238/101‑7 (MQ=17)
                                                                         atTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                            <  2:4033178/101‑1 (MQ=255)
                                                                         atTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                            <  1:486650/101‑1 (MQ=255)
                                                                          tttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                           >  2:483965/1‑101 (MQ=255)
                                                                           ttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                          >  2:2493033/1‑101 (MQ=255)
                                                                           ttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                          >  1:2084643/1‑101 (MQ=255)
                                                                            tgcaCCAAGGGGAAAGTGC.A.CCGCGGTTTATCTTTC.CCTTCGCCCAAAATAGCGGGGGGGGGGTCCCGGGGGGCGGGGGGCCAGCccccccctgcccccct                         <  2:701369/98‑1 (MQ=255)
                                                                            tGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                         <  2:1869782/101‑1 (MQ=255)
                                                                            tGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                         >  2:1877715/1‑101 (MQ=255)
                                                                            tGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                         >  2:1357515/1‑101 (MQ=255)
                                                                              gACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                       <  2:2260428/101‑1 (MQ=255)
                                                                               aCCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                      >  2:18351/1‑101 (MQ=255)
                                                                                ccAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTTACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                     >  1:3754295/1‑101 (MQ=255)
                                                                                ccAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                     <  1:696116/101‑1 (MQ=255)
                                                                                  aaGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                   <  2:1265873/101‑1 (MQ=255)
                                                                                  aaGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                   >  1:197615/1‑101 (MQ=255)
                                                                                  aaGGTGAAAGCAG.A.TCCCGGGTTGCCTTCCCCCTTGGCGT.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                   <  2:2470928/101‑1 (MQ=25)
                                                                                   aGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                  >  2:906569/1‑101 (MQ=255)
                                                                                    ggTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                 >  1:4109366/1‑101 (MQ=255)
                                                                                     gtaaacacAG.A.CCCCGGGTCCCTTTTCCCCTGGGGGC.AAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                <  2:831438/94‑1 (MQ=18)
                                                                                     gTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                >  2:3719064/1‑101 (MQ=255)
                                                                                         aaGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt            >  2:273278/1‑101 (MQ=255)
                                                                                           gCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa          >  1:1114466/1‑101 (MQ=255)
                                                                                            cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg         <  2:3983219/101‑1 (MQ=255)
                                                                                                     cGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt  >  2:1812113/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTT  >  NC_014034/3003941‑3004140

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG