BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,004,044	T→G	15.2%	intergenic (+90/+86)	gfo → / ← lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,004,044	0	T→G	15.2%	77.4 ‑0.2 ‑0.5	184	intergenic (+90/+86)	gfo/lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand): new base (16/12): ref base (85/71): total (101/83)
Fisher's exact test strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.25e-01

Only 100 of 214 total aligned reads displayed.


GCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGC  >  NC_014034/3003944‑3004144


gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGtt                                                                                                       <  2:1826187/101‑1 (MQ=255)


gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGtt                                                                                                       <  2:3854468/101‑1 (MQ=255)


 cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGttt                                                                                                      <  1:1173748/101‑1 (MQ=255)


 cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGttt                                                                                                      <  1:3947303/101‑1 (MQ=255)


 cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGttt                                                                                                      <  1:442173/101‑1 (MQ=255)


  atcttCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTg                                                                                                     <  1:491591/97‑1 (MQ=255)


   agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCCCCCCCCCGGGGATTTTGACCCCGGGGAAAGCAG.A.TCCCGGTTTGc                                                                                                    >  1:813646/1‑101 (MQ=25)


   agaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGc                                                                                                    <  2:3387834/101‑1 (MQ=255)


    gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                                   <  1:1460017/101‑1 (MQ=255)


    gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                                   <  1:3147018/101‑1 (MQ=255)


    gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                                   <  1:2106050/101‑1 (MQ=255)


     aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGTAAGCAG.A.TCCCGGTTTGCCt                                                                                                  <  2:2028812/101‑1 (MQ=255)


     aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCt                                                                                                  <  2:2878091/101‑1 (MQ=255)


       cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCttt                                                                                                <  2:1935779/101‑1 (MQ=255)


        tGAGGGCAAGGCGGGGGGCGCGGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTc                                                                                               <  1:359028/101‑1 (MQ=255)


             gCAAGGCGGGGGGCGCTTCCCCCCTCTTGGCCTGACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                          <  1:3512317/101‑1 (MQ=255)


             gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                          <  2:1582462/101‑1 (MQ=255)


              cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                         >  2:30909/1‑101 (MQ=255)


              cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                         <  1:330490/101‑1 (MQ=255)


              cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                         <  1:969716/101‑1 (MQ=255)


                    ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aa                                                                                  >  1:2298935/1‑101 (MQ=255)


                    ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aa                                                                                  >  2:2365582/1‑101 (MQ=255)


                    ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aa                                                                                  >  1:1682472/1‑101 (MQ=255)


                    ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGTATTTTGGCCAAAGGGAAAACAG.A.TCCCCGGTTGGCTTTCACCCTgggccca                                                                                   >  2:886641/1‑96 (MQ=16)


                      ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAat                                                                                >  1:4129647/1‑101 (MQ=255)


                      ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAat                                                                                >  2:260844/1‑101 (MQ=255)


                      ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAAGTCACCCCCCGGGATATTTGGACCGAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAat                                                                                <  1:3619473/101‑1 (MQ=255)


                       gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAata                                                                               <  1:1956319/101‑1 (MQ=255)


                         gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATc                                                                             <  1:3820137/101‑1 (MQ=255)


                            cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCg                                                                          <  1:3074594/101‑1 (MQ=255)


                             tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgg                                                                         <  1:2001901/101‑1 (MQ=255)


                             nnncccncTCTTGGCCTNACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgg                                                                         <  2:1718694/98‑1 (MQ=255)


                              gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggg                                                                        <  2:2763891/101‑1 (MQ=255)


                              gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggg                                                                        >  2:3446957/1‑101 (MQ=255)


                               ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgggg                                                                       <  2:2275491/101‑1 (MQ=255)


                                  cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGa                                                                    <  1:3033241/101‑1 (MQ=255)


                                   ccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAg                                                                   >  1:3679784/1‑101 (MQ=255)


                                       ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGTTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTccc                                                               <  2:982978/101‑1 (MQ=255)


                                       ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTccc                                                               <  1:695002/101‑1 (MQ=255)


                                          gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCcgcg                                                            >  2:4172714/1‑101 (MQ=255)


                                             tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCNGGGGGAGTCCCGCgggg                                                         >  2:930217/1‑101 (MQ=255)


                                              gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGa                                                        >  1:1265969/1‑101 (MQ=255)


                                               gcGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  2:1083159/100‑1 (MQ=255)


                                               aCGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  2:1955988/101‑1 (MQ=255)


                                               aCGGCCAATTCACCCCCCGGGATATTTGGACCAGGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  1:3668219/101‑1 (MQ=255)


                                               aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  1:2988739/101‑1 (MQ=255)


                                               aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  1:1068303/101‑1 (MQ=255)


                                               aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                       <  2:1230160/101‑1 (MQ=255)


                                                cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACg                                                      >  2:304157/1‑101 (MQ=255)


                                                   ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGCGTCCCGCGGGGACgggg                                                   >  2:2498185/1‑101 (MQ=255)


                                                   ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                   >  1:1567586/1‑101 (MQ=255)


                                                   ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                   >  2:1892372/1‑101 (MQ=255)


                                                     aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAAGATCCCGGGGGGAGTCCCGCGGGGACggggcg                                                 >  1:1011810/1‑99 (MQ=255)


                                                        tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                              <  2:3973737/101‑1 (MQ=255)


                                                        cccccccccGGGATTTTTGGCCCAAGGTGAAACCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCag                                              <  1:788784/98‑1 (MQ=18)


                                                        cccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTCGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCag                                              >  1:3787365/4‑101 (MQ=18)


                                                          cccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagcg                                            >  2:3122169/2‑99 (MQ=18)


                                                          cccccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                            <  2:2806912/100‑1 (MQ=255)


                                                           ccccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgc                                           >  2:2544382/1‑101 (MQ=255)


                                                            cccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                          >  1:1783337/1‑101 (MQ=18)


                                                             ccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                         <  1:4014542/101‑1 (MQ=255)


                                                              cccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                        <  1:2728365/101‑1 (MQ=18)


                                                              cccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                        <  2:1695780/101‑1 (MQ=255)


                                                                cGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                      >  1:2891537/1‑101 (MQ=18)


                                                                cGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                      >  1:3875948/1‑101 (MQ=18)


                                                                cGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                      >  2:302738/1‑101 (MQ=18)


                                                                 gggATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGGCGAgacccccc                                     >  1:701446/1‑100 (MQ=255)


                                                                  ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                    >  2:1014468/1‑99 (MQ=18)


                                                                   gATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                   >  2:3618145/1‑101 (MQ=255)


                                                                    atatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                  >  2:791856/1‑97 (MQ=18)


                                                                    atatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                  >  2:1573134/1‑97 (MQ=17)


                                                                     tatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                 >  2:509458/1‑96 (MQ=18)


                                                                      atTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                <  1:1937424/101‑7 (MQ=17)


                                                                      atTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                <  1:486650/101‑1 (MQ=255)


                                                                       tttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                               >  2:483965/1‑101 (MQ=255)


                                                                        ttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                              >  2:2493033/1‑101 (MQ=255)


                                                                         tgcaCCAAGGGGAAAGTGC.A.CCGCGGTTTATCTTTC.CCTTCGCCCAAAATAGCGGGGGGGGGGTCCCGGGGGGCGGGGGGCCAGCccccccctgcccccct                             <  2:701369/98‑1 (MQ=255)


                                                                         tGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                             <  2:1869782/101‑1 (MQ=255)


                                                                          ggACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                            <  2:2719153/101‑1 (MQ=255)


                                                                           gACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                           >  2:4029056/1‑101 (MQ=255)


                                                                           gACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                           <  2:2260428/101‑1 (MQ=255)


                                                                             ccAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTTACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                         >  1:3754295/1‑101 (MQ=255)


                                                                              cAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                        >  1:3453949/1‑101 (MQ=255)


                                                                               aaGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                       >  2:3339067/1‑101 (MQ=255)


                                                                               aaGGTGAAAGCAG.A.TCCCGGGTTGCCTTCCCCCTTGGCGT.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                       <  2:2470928/101‑1 (MQ=25)


                                                                                aGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                      >  2:906569/1‑101 (MQ=255)


                                                                                 ggTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                     >  2:4011900/1‑101 (MQ=255)


                                                                                  gtaaacacAG.A.CCCCGGGTCCCTTTTCCCCTGGGGGC.AAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                    <  2:831438/94‑1 (MQ=18)


                                                                                  gTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                    >  2:3719064/1‑101 (MQ=255)


                                                                                   tGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                   >  2:2629297/1‑101 (MQ=255)


                                                                                        gCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa              >  1:1114466/1‑101 (MQ=255)


                                                                                         cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg             >  2:1194635/1‑101 (MQ=255)


                                                                                         cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg             >  2:1150789/1‑101 (MQ=255)


                                                                                          aG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg            >  1:1636231/1‑101 (MQ=255)


                                                                                               tCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt         >  2:1133594/1‑101 (MQ=255)


                                                                                                 ccGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt       >  2:1756846/1‑101 (MQ=255)


                                                                                                     tttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg   <  2:1413035/101‑1 (MQ=255)


                                                                                                     tttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg   <  2:3808819/101‑1 (MQ=255)


                                                                                                     tttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg   <  1:2708594/101‑1 (MQ=255)


                                                                                                      ttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTAGGGCCGAGCGCTCAGGCGTGTTTGGc  >  2:1280641/1‑101 (MQ=255)


GCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGC  >  NC_014034/3003944‑3004144

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG