Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,048 C→A 18.2% intergenic (+94/+82) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0480C→A18.2% 87.7 ‑1.1 ‑0.0186intergenic (+94/+82)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (22/12):  ref base (84/68):  total (106/80)
Fisher's exact test strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.59e-01

Only 100 of 209 total aligned reads displayed.

GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148
                                                                                                      |                                                                                                     
gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                                       <  1:1460017/101‑1 (MQ=255)
gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                                       <  1:3147018/101‑1 (MQ=255)
gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGcc                                                                                                       <  1:2106050/101‑1 (MQ=255)
 aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCt                                                                                                      <  2:1909938/101‑1 (MQ=255)
 aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCt                                                                                                      <  2:2878091/101‑1 (MQ=255)
   cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAACAG.A.TCCCGGGTTGCCttt                                                                                                    >  2:2622012/1‑101 (MQ=255)
         gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCCCCtt                                                                                              >  2:4022869/1‑101 (MQ=255)
         gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                              <  2:1582462/101‑1 (MQ=255)
          cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                             <  1:969716/101‑1 (MQ=255)
           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTgg                                                                                            >  2:519589/1‑101 (MQ=255)
           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTgg                                                                                            >  2:3272129/1‑101 (MQ=255)
           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTgg                                                                                            <  1:3668326/101‑1 (MQ=255)
             ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGTATTTGGACCAAGGGGGAAGCAG.A.TCCCGGGTTGGCTTTCACCTTGgcg                                                                                          >  1:869379/1‑101 (MQ=37)
                ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aa                                                                                      >  1:2298935/1‑101 (MQ=255)
                ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aa                                                                                      >  2:2365582/1‑101 (MQ=255)
                ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGTATTTTGGCCAAAGGGAAAACAG.A.TCCCCGGTTGGCTTTCACCCTgggccca                                                                                       >  2:886641/1‑96 (MQ=16)
                 gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.aaa                                                                                     <  1:3284533/101‑1 (MQ=255)
                  ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAat                                                                                    >  1:4129647/1‑101 (MQ=255)
                  ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAat                                                                                    <  1:2718918/101‑1 (MQ=255)
                     gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATc                                                                                 <  1:3820137/101‑1 (MQ=255)
                      cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATcc                                                                                >  2:2708388/1‑101 (MQ=255)
                         tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgg                                                                             <  1:2001901/101‑1 (MQ=255)
                         nnncccncTCTTGGCCTNACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgg                                                                             <  2:1718694/98‑1 (MQ=255)
                          gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggg                                                                            >  2:3446957/1‑101 (MQ=255)
                          gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggg                                                                            >  2:1972173/1‑101 (MQ=255)
                          gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCggg                                                                            <  2:2763891/101‑1 (MQ=255)
                             ccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCgggggg                                                                         <  1:3458131/101‑1 (MQ=255)
                                  ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGGTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGGGTcc                                                                    >  1:3023382/1‑101 (MQ=255)
                                   ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGTTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTccc                                                                   <  2:982978/101‑1 (MQ=255)
                                   gatctcTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTccc                                                                   <  1:1942635/96‑1 (MQ=255)
                                      gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCcgcg                                                                >  2:4172714/1‑101 (MQ=255)
                                        cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTcccagatc                                                              >  2:1942509/1‑96 (MQ=255)
                                        cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCggg                                                              >  2:486593/1‑101 (MQ=255)
                                         tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCNGGGGGAGTCCCGCgggg                                                             >  2:930217/1‑101 (MQ=255)
                                          gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGa                                                            >  1:1265969/1‑101 (MQ=255)
                                           aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                           <  2:1230160/101‑1 (MQ=255)
                                           aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGAc                                                           <  1:164420/101‑1 (MQ=255)
                                            cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACg                                                          >  2:304157/1‑101 (MQ=255)
                                             ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                         >  2:309383/1‑101 (MQ=255)
                                              gCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAT.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACggg                                                        <  1:2481845/101‑1 (MQ=255)
                                               ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGCGTCCCGCGGGGACgggg                                                       >  2:2498185/1‑101 (MQ=255)
                                               ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                       >  1:1567586/1‑101 (MQ=255)
                                                cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                      >  1:688360/1‑101 (MQ=255)
                                                 aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAAGATCCCGGGGGGAGTCCCGCGGGGACggggcg                                                     >  1:1011810/1‑99 (MQ=255)
                                                  aTTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACggggggg                                                    >  1:3573604/1‑100 (MQ=255)
                                                  aTTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    >  1:1785884/1‑101 (MQ=255)
                                                    tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                  >  1:1118269/1‑101 (MQ=255)
                                                    cccccccccGGGATTTTTGGCCCAAGGTGAAACCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCag                                                  <  1:788784/98‑1 (MQ=18)
                                                     ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 >  1:1692523/3‑100 (MQ=18)
                                                     ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                 <  2:3829516/99‑2 (MQ=18)
                                                      cccccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                <  2:2806912/100‑1 (MQ=255)
                                                      aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                >  1:4191610/1‑101 (MQ=255)
                                                        cccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                              >  1:3742053/1‑101 (MQ=18)
                                                         ccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCGCGATTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                             <  1:3619766/101‑1 (MQ=255)
                                                         ccccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                             <  1:4014542/101‑1 (MQ=255)
                                                          cccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                            <  2:2239536/101‑1 (MQ=17)
                                                          cccGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                            <  2:1695780/101‑1 (MQ=255)
                                                           ccGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                           >  1:3050226/1‑101 (MQ=18)
                                                            cGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                          >  1:3875948/1‑101 (MQ=18)
                                                            cGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                          >  2:302738/1‑101 (MQ=18)
                                                              ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                        >  2:1014468/1‑99 (MQ=18)
                                                               gATTTTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                       <  1:3595515/101‑1 (MQ=255)
                                                               gATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                       >  2:2587037/1‑98 (MQ=18)
                                                               gATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                       >  2:3618145/1‑101 (MQ=255)
                                                                atatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                      >  2:791856/1‑97 (MQ=18)
                                                                atatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                      >  2:1573134/1‑97 (MQ=17)
                                                                 tatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                     <  2:2260526/101‑6 (MQ=17)
                                                                 tatTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                     <  1:293129/101‑1 (MQ=255)
                                                                 tatTTGGACCAAGGTGAAAGCAG.A.ACCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGCGTCCCGCGGGGACGGGGGGCACAgcccccctgccc                                     >  1:3425444/1‑101 (MQ=255)
                                                                  atTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGG.AAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                    <  2:3860238/101‑7 (MQ=17)
                                                                  atTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                    <  2:4033178/101‑1 (MQ=255)
                                                                  atTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                    <  1:486650/101‑1 (MQ=255)
                                                                    ttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  <  2:1130864/101‑1 (MQ=255)
                                                                    ttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  >  2:2493033/1‑101 (MQ=255)
                                                                     tgcaCCAAGGGGAAAGTGC.A.CCGCGGTTTATCTTTC.CCTTCGCCCAAAATAGCGGGGGGGGGGTCCCGGGGGGCGGGGGGCCAGCccccccctgcccccct                                 <  2:701369/98‑1 (MQ=255)
                                                                     tGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                 >  2:1877715/1‑101 (MQ=255)
                                                                        aCCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                              >  2:18351/1‑101 (MQ=255)
                                                                         ccAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                             <  1:696116/101‑1 (MQ=255)
                                                                           aaGGTGAAAGCAG.A.TCCCGGGTTGCCTTCCCCCTTGGCGT.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                           <  2:2470928/101‑1 (MQ=25)
                                                                            aGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                          >  2:906569/1‑101 (MQ=255)
                                                                              gtaaacacAG.A.CCCCGGGTCCCTTTTCCCCTGGGGGC.AAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                        <  2:831438/94‑1 (MQ=18)
                                                                              gTGAGAGCAG.A.TCCCGGTTTGCCTTCCACCTGGGCGC.AAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                        <  1:2890775/101‑1 (MQ=37)
                                                                              gTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                        >  2:3719064/1‑101 (MQ=255)
                                                                                gAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                      >  2:3363976/1‑101 (MQ=255)
                                                                                  aaGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                    >  2:273278/1‑101 (MQ=255)
                                                                                    gCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                  >  1:1075178/1‑101 (MQ=255)
                                                                                     cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                 <  2:3983219/101‑1 (MQ=255)
                                                                                     cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                 >  2:1194635/1‑101 (MQ=255)
                                                                                     cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                 >  2:1150789/1‑101 (MQ=255)
                                                                                      aG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  1:1636231/1‑101 (MQ=255)
                                                                                         a.TCCCGGTTTGCCTTTCACCTTGGCGC.AAACATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg              >  1:3763122/1‑101 (MQ=255)
                                                                                             ccGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt           >  2:1756846/1‑101 (MQ=255)
                                                                                                 tttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       <  2:3808819/101‑1 (MQ=255)
                                                                                                 tttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       <  2:1413035/101‑1 (MQ=255)
                                                                                                 tttGCCTTCCCCCTGGGGAA.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       <  2:22920/101‑1 (MQ=25)
                                                                                                  ttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTAGGGCCGAGCGCTCAGGCGTGTTTGGc      >  2:1280641/1‑101 (MQ=255)
                                                                                                  ttGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTACCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgtc      >  2:2654896/1‑99 (MQ=255)
                                                                                                   tGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg     >  2:458026/1‑101 (MQ=255)
                                                                                                      cTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc  >  1:368530/1‑101 (MQ=255)
                                                                                                      cTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc  >  1:1037565/1‑101 (MQ=255)
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GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGC.AAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG