Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,011,287 A→T 86.3% intergenic (+86/+56) purK → / ← RCAP_rcc02822 phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,011,2870A→T86.3% 79.3 0.0 0.0182intergenic (+86/+56)purK/RCAP_rcc02822phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Reads supporting (aligned to +/- strand):  new base (90/67):  ref base (14/11):  total (104/78)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379
                                                                                              |                                                                                            
ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgg                                                                                       >  1:1055362/1‑101 (MQ=255)
 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggg                                                                                      <  2:2657299/101‑1 (MQ=255)
  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggg                                                                                     >  2:4185809/1‑101 (MQ=255)
  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggg                                                                                     >  1:2101410/1‑101 (MQ=255)
         cgcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACt                                                                              >  1:3313436/1‑101 (MQ=255)
          gcgGCAGCGGGGGCTGTCTGCCCCCGCCCCCCCGAGGATATTTCCGCCCAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACtg                                                                             >  2:610851/1‑100 (MQ=38)
          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACtt                                                                             <  2:2900949/101‑1 (MQ=255)
          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACtt                                                                             <  1:3867532/101‑1 (MQ=255)
          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACtt                                                                             >  1:3081722/1‑101 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTg                                                                            <  1:1859066/101‑1 (MQ=255)
             gCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGcc                                                                          >  2:3107148/1‑101 (MQ=255)
              cAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCg                                                                         >  1:733467/1‑101 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGc                                                                        <  1:2430148/101‑1 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCACTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGc                                                                        >  2:3835273/1‑101 (MQ=37)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                      <  2:739895/101‑1 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                     >  2:1843129/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                    >  1:3439963/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                    >  1:3253946/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                    >  1:1557016/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                    <  2:1881368/101‑1 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                 <  1:611103/101‑1 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                 <  1:2491847/101‑1 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAag                                                                 <  1:983552/101‑1 (MQ=255)
                       cTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAaga                                                                <  1:2655579/101‑1 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAagag                                                               <  2:3651790/101‑1 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                               >  2:511254/1‑101 (MQ=255)
                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                              >  2:4092587/1‑101 (MQ=255)
                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                              >  2:2095536/1‑101 (MQ=255)
                          tCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggg                                                             <  2:921976/101‑1 (MQ=255)
                            tGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAggggg                                                           <  2:3032098/101‑1 (MQ=255)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGCGACTTGCCGCAGGCAAGGgggggg                                                          >  2:1139558/1‑101 (MQ=38)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                          >  1:1919833/1‑101 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                        <  1:1483085/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                        <  2:3384621/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                        <  2:2225676/101‑1 (MQ=255)
                                cccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg                                                       >  2:1213091/1‑101 (MQ=255)
                                 ccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgc                                                      <  1:2970379/101‑1 (MQ=255)
                                  cGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcg                                                     <  1:4085084/101‑1 (MQ=255)
                                    cACCCCCGAGGATAGTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCCGCGcc                                                   >  2:382300/1‑101 (MQ=37)
                                       ccccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGccccc                                                >  2:540012/1‑101 (MQ=255)
                                        cccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccccc                                               >  2:3622086/1‑101 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              >  2:3244859/1‑101 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              >  2:3402049/1‑101 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             >  2:1356992/1‑101 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             >  2:1045354/1‑101 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             <  1:3994899/101‑1 (MQ=255)
                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTg                                             <  2:2809828/101‑1 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                            >  1:2362729/1‑101 (MQ=255)
                                            aGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGcc                                           >  1:2777882/1‑101 (MQ=255)
                                             ggATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGccc                                          >  1:175283/1‑101 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                         <  1:3511415/101‑1 (MQ=255)
                                                tatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                       >  2:3901475/1‑101 (MQ=255)
                                                tatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                       >  1:2675599/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                      >  2:954354/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                      >  2:3310627/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                      >  1:2960356/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                      >  1:95356/1‑101 (MQ=255)
                                                    tcggCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATGTCCCGGGGGGAGACTTGCCGCGGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                   <  2:2315944/98‑1 (MQ=37)
                                                    tCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                   <  2:2413934/101‑1 (MQ=255)
                                                     ccGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACg                                  <  2:2146296/101‑1 (MQ=255)
                                                     ccGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACg                                  <  2:3446667/101‑1 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                 <  1:1824018/101‑1 (MQ=255)
                                                        ccAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCa                               <  2:554104/101‑1 (MQ=255)
                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                              <  1:1321176/101‑1 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                             >  2:732798/1‑101 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                             >  1:3784417/1‑101 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                             <  1:1352219/101‑1 (MQ=255)
                                                             gTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                          <  1:483402/101‑1 (MQ=255)
                                                             gTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                          >  1:805718/1‑101 (MQ=255)
                                                             gTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                          >  2:3713146/1‑101 (MQ=255)
                                                              tGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         >  1:1633550/1‑101 (MQ=255)
                                                               gAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAg                        >  2:1547947/1‑101 (MQ=255)
                                                               gAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAg                        >  2:2647245/1‑101 (MQ=255)
                                                                aaaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCCGc                       >  1:2728468/1‑101 (MQ=37)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                      >  2:2779623/1‑101 (MQ=255)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAAcc                      >  2:1705415/1‑101 (MQ=37)
                                                                  aacaacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                     >  1:4042548/1‑101 (MQ=255)
                                                                  aacaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                     <  1:3717350/101‑1 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  2:2863990/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  1:3165518/1‑101 (MQ=255)
                                                                   acaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGc                    >  2:1587182/1‑101 (MQ=255)
                                                                    caacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTCCCCGGCTCACGGCAGGGCAAGCCGCt                   >  2:472055/1‑101 (MQ=25)
                                                                      acGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                 >  1:3062755/1‑101 (MQ=255)
                                                                       cGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCa                >  2:3504322/1‑101 (MQ=255)
                                                                        gCCCTTCCCCCTTGGGAG.AAATTTCCCGGGGGGGGACTTGCCGCAGGCAAGGGGGGGGCGGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa               <  2:3264769/101‑1 (MQ=25)
                                                                          ccTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCCAgg             >  2:2746348/1‑101 (MQ=37)
                                                                            tttCTCCTGGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCCCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGcc           <  2:198567/101‑1 (MQ=25)
                                                                            tttCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGcc           >  2:3168128/1‑101 (MQ=255)
                                                                              tCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc         >  1:2370864/1‑101 (MQ=255)
                                                                               cACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgcg        >  1:1843553/1‑101 (MQ=255)
                                                                               cACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgcg        >  2:2540922/1‑101 (MQ=255)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc       >  2:23972/1‑101 (MQ=255)
                                                                                  cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGccc     >  1:3756611/1‑101 (MQ=255)
                                                                                  cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGccc     >  1:655008/1‑101 (MQ=255)
                                                                                  cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGAAGCGCCCCCCTGCCCGGCTCACGGCCGGGCCAGCCCCTGCAAGGCCGCGccc     >  1:1057334/1‑101 (MQ=37)
                                                                                   ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc    >  1:1182970/1‑101 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   >  2:3735327/1‑101 (MQ=255)
                                                                                     gggAC.AAATCTCCCGGGGGGAGATTTGCGGCAGGCAAGAGGGGGGCAGCGCCCCCCGCCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg  <  2:991724/101‑1 (MQ=25)
                                                                                     gggAC.AAATATCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg  <  2:2961438/101‑1 (MQ=37)
                                                                                     gggAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg  <  1:44795/101‑1 (MQ=255)
                                                                                              |                                                                                            
TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG