Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,042,953 G→T 100% W217L (TGG→TTG)  thiD → phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,042,9530G→T100.0% 694.3 180W217L (TGG→TTG) thiDphosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  new base (103/77):  ref base (0/0):  total (103/77)

Only 100 of 187 total aligned reads displayed.

CGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTGGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTTCG  >  NC_014034/3042855‑3043050
                                                                                                  |                                                                                                 
cGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTtgg                                                                                                 >  2:1998657/1‑101 (MQ=255)
     tgctgcTCGATGCCGGGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACg                                                                                            <  1:3841614/101‑1 (MQ=255)
     tgctgcTCGATGCCGCGGGCGAGGCCTGGCTCGAGGGGCCGGGGGTCGAGACGAAAAAAACCCATGGCACCGGCTCCACCCCGTCTTCGGCCTTGGCgccc                                                                                            >  1:3310246/1‑98 (MQ=255)
       ctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCa                                                                                          >  1:1666413/1‑101 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                         >  1:2796030/1‑101 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                         <  2:8753/101‑1 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                         <  1:3823825/101‑1 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                         <  1:1750426/101‑1 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    <  1:281210/101‑1 (MQ=255)
             gATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCAGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGc                                                                                    >  2:922905/1‑101 (MQ=255)
              aTGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGcc                                                                                   <  2:3036674/101‑1 (MQ=255)
              aTGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGcc                                                                                   >  2:1646938/1‑101 (MQ=255)
               tGCGGCGGGCGAGGTCTGGTTCGGGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGccc                                                                                  <  2:484577/101‑1 (MQ=255)
                gCCGCGGGCGAGGTCTGGTTCGAGGCACCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCg                                                                                 >  2:3324151/1‑101 (MQ=255)
                  cgcgGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCa                                                                               >  1:1439798/1‑101 (MQ=255)
                   gcgGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCAt                                                                              >  2:795807/1‑101 (MQ=255)
                    cgGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATc                                                                             >  1:2845001/1‑101 (MQ=255)
                     gggCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATcc                                                                            >  1:1993832/1‑101 (MQ=255)
                     gggCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATcc                                                                            <  2:3615950/101‑1 (MQ=255)
                     gggCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATcc                                                                            >  1:2154090/1‑101 (MQ=255)
                       gCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATcccc                                                                          >  1:2469557/1‑101 (MQ=255)
                        cGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCg                                                                         >  2:2483553/1‑101 (MQ=255)
                        cGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCg                                                                         <  1:746924/101‑1 (MQ=255)
                        cGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCg                                                                         <  1:934411/101‑1 (MQ=255)
                        cGAGGGGTGGTTCGAGGGGCCGGGGGTCGAGACGAAAAACATCCATGAAACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCg                                                                         >  2:719170/1‑101 (MQ=255)
                          aGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGAt                                                                       <  2:2105987/101‑1 (MQ=255)
                            gTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCt                                                                     >  1:2951283/1‑101 (MQ=255)
                            gTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCt                                                                     >  1:1824151/1‑101 (MQ=255)
                             tCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTg                                                                    <  1:53634/101‑1 (MQ=255)
                              cTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgc                                                                   <  1:2084550/101‑1 (MQ=255)
                                ggTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgcgc                                                                 >  2:3025057/1‑101 (MQ=255)
                                 gTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGcc                                                                >  1:430246/1‑101 (MQ=255)
                                  ttCGAGGCCCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCa                                                               <  2:1943169/101‑1 (MQ=255)
                                    cGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAgg                                                             <  1:1302068/101‑1 (MQ=255)
                                    cGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAgg                                                             >  1:1510904/1‑101 (MQ=255)
                                     gAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgc                                                            >  1:4122938/1‑101 (MQ=255)
                                         cgccgcGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgcc                                                        >  1:3547246/1‑101 (MQ=255)
                                          gccgcGGGTCGAGACGAAAAACCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccg                                                       <  2:1219335/101‑1 (MQ=255)
                                            cgcgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgcc                                                     <  1:2772541/101‑1 (MQ=255)
                                              cgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgc                                                   >  1:2357738/1‑101 (MQ=255)
                                              cgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgc                                                   >  1:3458562/1‑101 (MQ=255)
                                               gggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgcc                                                  >  2:318729/1‑101 (MQ=255)
                                               gggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgcc                                                  >  2:2979104/1‑101 (MQ=255)
                                               gggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgcc                                                  <  2:561634/101‑1 (MQ=255)
                                                ggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgccg                                                 <  1:2116101/101‑1 (MQ=255)
                                                 gTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgccgc                                                >  2:2749129/1‑101 (MQ=255)
                                                  tCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgccgcc                                               <  2:3441710/101‑1 (MQ=255)
                                                   cGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgccc                                              >  1:2222727/1‑101 (MQ=255)
                                                   cGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgccc                                              >  1:3216779/1‑101 (MQ=255)
                                                   cGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgccc                                              <  2:2357562/101‑1 (MQ=255)
                                                   cGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgccc                                              >  2:1058063/1‑101 (MQ=255)
                                                    gagaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgcccg                                             >  1:2602601/1‑101 (MQ=255)
                                                     agaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGACATGCGCCAGGCCGCCGCCGccgcccgc                                            >  1:192213/1‑101 (MQ=255)
                                                      gaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCcgcg                                           >  2:3204475/1‑101 (MQ=255)
                                                         gAAAAACCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCt                                        <  1:825834/101‑1 (MQ=255)
                                                         gAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCgcgcgc                                        >  1:1681089/1‑99 (MQ=255)
                                                           aaaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtat                                      <  2:3796624/101‑1 (MQ=255)
                                                           aaaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtat                                      >  1:3130483/1‑101 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                     >  2:1038002/1‑101 (MQ=255)
                                                             aaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCCCCGCCCGCGCCTATAc                                    <  1:3697342/101‑1 (MQ=255)
                                                               cacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGc                                  >  2:950584/1‑101 (MQ=255)
                                                               cacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGc                                  >  1:4131774/1‑101 (MQ=255)
                                                                ccccATGGCTCCGGCTGCTCCCTGTCTTCGGCCTTGGCGACGCAGCTGTCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCt                                 <  2:1081452/100‑1 (MQ=255)
                                                                ccccATGGCACCGGCTGCCCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCt                                 <  1:3336699/100‑1 (MQ=255)
                                                                  ccATGGCACCGGCTGCGCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgc                               <  1:1086767/101‑1 (MQ=255)
                                                                    aTGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgc                             >  2:4050001/1‑101 (MQ=255)
                                                                    aTGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgc                             >  2:1709634/1‑101 (MQ=255)
                                                                    aTGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgc                             >  2:1966629/1‑101 (MQ=255)
                                                                     tGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgcg                            <  1:2763909/101‑1 (MQ=255)
                                                                       gCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGcc                          >  1:3696484/1‑101 (MQ=255)
                                                                        cACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCa                         <  1:452689/101‑1 (MQ=255)
                                                                         aCCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCAt                        >  1:3980791/1‑101 (MQ=255)
                                                                         aCCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCAt                        >  1:3971606/1‑101 (MQ=255)
                                                                         aCCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCAt                        >  2:4160520/1‑101 (MQ=255)
                                                                         aCCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCAt                        <  2:4232328/101‑1 (MQ=255)
                                                                           cGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcg                      <  2:753863/101‑1 (MQ=255)
                                                                            ggCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgc                     >  2:2880051/1‑101 (MQ=255)
                                                                             gCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgcc                    >  1:2516091/1‑101 (MQ=255)
                                                                              cTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccg                   >  2:2783182/1‑101 (MQ=255)
                                                                               tctaCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                  <  1:767805/98‑1 (MQ=255)
                                                                               tGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                  >  1:1013621/1‑101 (MQ=255)
                                                                               tGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                  >  1:3669292/1‑101 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGGTCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 <  2:1945018/101‑1 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 >  1:1707770/1‑101 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCGGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                 <  2:3971346/101‑1 (MQ=255)
                                                                                 cACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCgg                <  1:3147310/101‑1 (MQ=255)
                                                                                  aCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc               >  2:3065633/1‑101 (MQ=255)
                                                                                  aCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc               >  2:3103715/1‑101 (MQ=255)
                                                                                   cccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGcc              <  2:2902710/101‑1 (MQ=255)
                                                                                       gTCTTCGGCCTTGGCGACGCAGCTGTCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGAcc          <  2:2668684/101‑1 (MQ=255)
                                                                                       gTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGAcc          >  2:3753695/1‑101 (MQ=255)
                                                                                        tCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCTCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCg         <  2:210281/101‑1 (MQ=255)
                                                                                        tCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCg         <  2:1687211/101‑1 (MQ=255)
                                                                                          ttCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGc       >  2:3654658/1‑101 (MQ=255)
                                                                                          ttCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGc       <  1:248662/101‑1 (MQ=255)
                                                                                             ggCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCttt    <  2:573073/101‑1 (MQ=255)
                                                                                             ggCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCttt    >  1:1589590/1‑101 (MQ=255)
                                                                                              gCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTtc   <  1:1038093/101‑1 (MQ=255)
                                                                                               ccTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTtcg  >  2:1420410/1‑101 (MQ=255)
                                                                                               ccTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTtcg  <  1:2914724/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTGGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTTCG  >  NC_014034/3042855‑3043050

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG