Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,179 A→T 3.9% intergenic (‑162/+130) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1790A→T 3.9% 4.1 ‑0.9 0.0146intergenic (‑162/+130)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (83/56):  total (89/57)
Fisher's exact test strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279
                                                                                                       |                                                                                                           
cGATGGGCAGGCTGGCCGATGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCa                                                                                                             <  1:2377050/101‑1 (MQ=255)
cGATGGGCAGGCGGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCa                                                                                                             <  2:2030896/101‑1 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCAt                                                                                                            <  1:1533616/101‑1 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCAt                                                                                                            >  1:1475925/1‑101 (MQ=255)
 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCAt                                                                                                            >  2:2366800/1‑101 (MQ=255)
  aggggCGGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  1:1774654/99‑1 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           >  2:2827143/1‑101 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  1:1065946/101‑1 (MQ=255)
  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  1:1729808/101‑1 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGTCCCGCCCATggc                                                                                                         <  1:2289102/101‑1 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc                                                                                                         <  1:122909/101‑1 (MQ=255)
    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc                                                                                                         <  2:3984774/101‑1 (MQ=255)
     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.g                                                                                                       >  1:1920046/1‑101 (MQ=255)
      gCAGGCTGACCGAGGCCATCGCCCAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gg                                                                                                      >  2:744042/1‑101 (MQ=255)
       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.ggg                                                                                                     >  2:1167520/1‑101 (MQ=255)
       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.ggg                                                                                                     <  1:3509296/101‑1 (MQ=255)
        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gggg                                                                                                    >  2:2595586/1‑100 (MQ=255)
        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gggc                                                                                                    >  2:1473617/1‑101 (MQ=255)
         ggCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.ggggg                                                                                                   >  1:1984297/1‑99 (MQ=255)
            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.Ctt                                                                                               <  2:3120389/101‑1 (MQ=255)
            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCACGGC.GGggg.cgt                                                                                               >  2:2022118/1‑99 (MQ=255)
             gACCGAGGCGATCGCCGAGGTCATGCGGACCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtc                                                                                              <  1:329548/101‑1 (MQ=255)
              aCCGAGGCCATCGCCGAGGTCAGGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtct                                                                                             <  2:4218580/101‑1 (MQ=255)
               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtctc                                                                                            >  2:3879033/1‑101 (MQ=255)
               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtctc                                                                                            <  2:1065203/101‑1 (MQ=255)
                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTcgc.c                                                                                        >  1:604899/1‑101 (MQ=255)
                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTccc.c                                                                                        >  1:227116/1‑101 (MQ=255)
                     ccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCCCCCCCCCCCTGGG.GGGCG.GGTCTcgc.cccc                                                                                     >  1:1173776/1‑99 (MQ=18)
                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTcgc.cc.g.cc                                                                                  <  2:2664845/101‑1 (MQ=255)
                         cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCCC.CC.G.CCc.gg                                                                              >  2:2306011/1‑101 (MQ=255)
                          gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCCTGGC.GGGCG.CTTCTCGC.CC.C.CCg.ggg                                                                             >  2:3830834/1‑101 (MQ=255)
                           ccGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGGG.CCTCTCCC.CC.C.CCG.gggc                                                                            >  1:79698/1‑101 (MQ=18)
                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCCCCCATGGC.GGGCG.CTTCGCCC.CC.T.GCg.ggggg                                                                           >  1:758458/1‑99 (MQ=17)
                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.gggcgg                                                                          <  1:2595777/101‑1 (MQ=255)
                               ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.gggcgggc                                                                        <  2:1833609/101‑1 (MQ=255)
                                gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.Gggcgggcg                                                                       <  2:2439155/101‑1 (MQ=255)
                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGGG.CGTCTCCC.CC.G.CCC.gggcggggggc                                                                     >  1:2080720/1‑97 (MQ=18)
                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGGGcc                                                                     >  2:3126655/1‑101 (MQ=255)
                                     gcgcgaCAATGGCCCCGTGACCGGCGAGGGCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGGGCCGGCCCCC.CC.G.CCG.GGGGGGGGGGCgg                                                                   >  2:1595751/6‑101 (MQ=17)
                                     gCGGACCAGTGGCCTCGTGCCCCCCGAGTGCCCAAAGC.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGc                                                                  <  1:1685015/101‑1 (MQ=17)
                                      cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCCGcc                                                                 >  2:1378869/1‑101 (MQ=255)
                                       ggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCC.GGGCGGGCGGCGGccc                                                                >  2:2425929/1‑100 (MQ=255)
                                          acgggggCCTCGGGCCCGCCGAGGCCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCT.GGGCGGGCGCCGGCCTctg                                                             <  2:2195308/96‑1 (MQ=14)
                                          acatggcccccGTGCCCGCGGAGGGCCAAAAGG.CCCTC.GGCCAGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTctg                                                             <  2:898105/91‑1 (MQ=11)
                                          aCAATGGCCCCGTGACCGGCGAGGGCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGGG.C.GGGCGCGCC.G.CCG.GGGCGGGGGGCgggggcct                                                             >  1:1355827/1‑95 (MQ=17)
                                           cAGTGCCCTCGGGCCCCCCGAGGCCCCAAGGC.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTctgc                                                            <  2:1016424/101‑1 (MQ=14)
                                              tGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCtgctgc                                                         >  2:251806/1‑101 (MQ=255)
                                                  cTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGTGCCGGCCCCCGCTTCGccc                                                     >  1:98066/1‑100 (MQ=14)
                                                  cTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCCGCCTCTGCTGCGCC.t                                                    >  2:3459419/1‑101 (MQ=255)
                                                  cTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CCTCTCGC.CC.G.CCG.GGGCGGGCGCCGGGCCCTGCTGCGCC.t                                                    >  2:533275/1‑101 (MQ=255)
                                                    cgggcccgccgGGGGCCCAAGGG.CCCTC.GGCCAGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.Tgg                                                  <  2:4160271/96‑1 (MQ=14)
                                                    cGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.C.CCG.GGGGGGGGGCCGGCCTCTGCTGCGCC.Tgg                                                  >  2:2699520/1‑101 (MQ=255)
                                                     gTGACCGCCGGGGGCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGCGGGGGG.CTTCGCG..CC.G.CCGCGGGCGGGGGGCGGCCGCCGCGgcgggtgg                                                   >  2:3553242/1‑96 (MQ=2)
                                                      tgcccgccgAGGGCCCAAGGG.CCCCC.GGCCGGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.Tgggg                                                <  2:3292370/98‑1 (MQ=14)
                                                         ccgccgGGGGCCCAAAGG.CCCCC.GGCCAGGC.CCCGCCCCGCCCATGGC.GGGCC.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGaaa                                             <  1:2784782/101‑1 (MQ=14)
                                                          cgccgAGGGCCCAAGGC.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAAc                                            <  1:1826126/101‑1 (MQ=255)
                                                          cccccggggCCCAAACG.CCCCCGGGCCGGCC.CCCGCCCCGCCCTGGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGaaa                                             <  2:1900966/95‑1 (MQ=14)
                                                             ggcggccccAAGGG.CCCCC.GGCAAGCC.CCCCCCCCGCCGATGGC.GGGCG.TTTCTGGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGt                                         <  1:2389371/95‑1 (MQ=17)
                                                             cggggccccAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGt                                         <  2:1096055/95‑1 (MQ=255)
                                                             cgAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGGGCCGGCCTCTGCTGCGCC.CGGGGGAACggg                                         >  1:1096149/1‑100 (MQ=255)
                                                             cgAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCCCTGCTGCGCC.TGGGGGAACggg                                         >  1:1454992/1‑100 (MQ=255)
                                                              gAGGGCCCAAAGG.CCCTC.GGCGAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGGNCCCGCCTCTGCTGCGGC.TGGGGGAACgggc                                        >  1:2410580/1‑99 (MQ=14)
                                                              gAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGGG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCCGCCGCGCC.TGGGGGAACgggc                                        >  1:2479361/1‑99 (MQ=14)
                                                              gAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGGGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                        >  2:3358883/1‑101 (MQ=255)
                                                              gAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGGAACGGTc                                        >  1:4051850/1‑101 (MQ=255)
                                                               ggggCCCCAAGGCCCCCC.GCCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                        <  1:2366976/100‑1 (MQ=17)
                                                               gaggCCCAAAGGCCCCTC.GGCCGGCC.CCCGNCCCGCCCAGGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                        <  1:195563/99‑1 (MQ=17)
                                                               gaggCCCAAAGG.CCCCCGGGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                        <  2:2545566/99‑1 (MQ=17)
                                                                gggCCCAAGGG.CCCCC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCtt                                      <  2:2006297/101‑1 (MQ=255)
                                                                gggCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGCG.CGGTCCGC.CCGG.CCC.GGGCGGGGGGGGGCCCCCGCCGTGCCTTGGGGGAACgggc                                        >  1:1114979/1‑99 (MQ=2)
                                                                 ggCCCAAGGG.CCCTC.GGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTg                                     <  1:3459671/101‑1 (MQ=255)
                                                                 ggCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGCC.CTGGCCGC.GC.GCCCG.GGGGGGGCGCCCGCCCCTTCTCCCGC.Tggggtgatcgggtc                                      >  2:2935939/1‑91 (MQ=2)
                                                                 gccccAAAGC.CCCTC.GGCCGGCC.CCCCCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCCGCTGCGCC.TGGGGAAACGGTCTTg                                     <  1:3696279/99‑1 (MQ=18)
                                                                   gccAAAGT.CCCCT.GGCCCGCCCCCCGCCCCGCCCATGGC.GGGCG.CTTCGCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTgg                                    <  2:4130183/100‑1 (MQ=14)
                                                                      agcgg.CCCCC.GGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCAcc                                <  1:4228933/98‑1 (MQ=18)
                                                                       aggg.CCCTC.GGCCGGCC.CCCGCCCCGCCTATGGC.GGGCG.CTTCTCGC.CC.G.CGG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCg                               <  1:2532910/99‑1 (MQ=18)
                                                                        aggccccTC.GGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CGG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCg                               <  1:4151015/97‑1 (MQ=18)
                                                                        aGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.CGGGGAAACGGTCTTGGCCCCGc                              >  1:4187860/1‑101 (MQ=255)
                                                                          g.CCCTC.GCCCAGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGccc                            <  2:2146985/101‑1 (MQ=255)
                                                                                c.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGGGCCGGCCCCCGCTGCGCC.TGGGGGAACGGGCTTGGCACCGCCCGGGcc                       >  1:2878136/1‑100 (MQ=18)
                                                                                c.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGAACCCGCCTCTGCGGCGGC.TGGGGAAAAGGTGTTGGCACCCCCCGGGcc                       >  1:4005573/1‑100 (MQ=18)
                                                                                  ggCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGGAACGGTCTTGGCACCGCCCGGGCTc                      >  1:2390801/1‑101 (MQ=255)
                                                                                  ggCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTc                      >  2:3260323/1‑101 (MQ=255)
                                                                                   gccccgcccccgccccgcCCAGGGC.GGGCG.TTTCCGGC.CC.G.CGG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTc                      <  1:3879292/93‑1 (MQ=18)
                                                                                    ccAGCG.CCCCCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CGG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg                    <  2:1355698/101‑1 (MQ=255)
                                                                                     cAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGGGCCGGCCTCTGCTGCGCC.CGGGGGAACGGGCTTGGCACCGCCCGGGCTCcgg                   >  1:2984749/1‑101 (MQ=21)
                                                                                     cAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCcgg                   >  1:1311981/1‑101 (MQ=255)
                                                                                       gcg.cCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCcggcg                 >  2:2786053/1‑101 (MQ=255)
                                                                                         g.cccgcccCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAANCGGTCTTGGCACCGCCCGGGCTCCggcggg               >  1:1057335/1‑101 (MQ=255)
                                                                                             ccccccgcccCTGGC.GGCCG.CTTCGCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            <  1:3193197/99‑1 (MQ=255)
                                                                                             ccccccgcccAGGGG.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            <  1:2727216/99‑1 (MQ=255)
                                                                                             ccccccgcccAGGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACCGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            <  1:3773571/99‑1 (MQ=255)
                                                                                                ccggcccATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa         <  1:711033/98‑1 (MQ=255)
                                                                                                cccgcccAGGGG.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa         <  2:2684698/101‑1 (MQ=255)
                                                                                                 ccgcccGTGGC.GGGCG.TTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa        <  2:565013/101‑1 (MQ=255)
                                                                                                 ccgcccATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCCGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa        >  1:3964315/1‑101 (MQ=255)
                                                                                                     ccATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc    >  1:1381241/1‑101 (MQ=255)
                                                                                                     ccATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAAGc    >  2:2823067/1‑101 (MQ=255)
                                                                                                     ccATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGGCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc    >  1:1493012/1‑101 (MQ=255)
                                                                                                       aTGGC.GCGCG.CTTCTCGC.GC.G.CCC.GGGCGGCGACCGGGCCTTGTTGCACC.TGGGGTAACGGTCTTTGCGCCCCCTAGGCTCCGGCGGGCggggccagctag  >  2:685563/1‑93 (MQ=255)
                                                                                                       |                                                                                                           
CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCG.GGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279

Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG