Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,180 T→C 3.5% intergenic (‑163/+129) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1800T→C 3.5% 4.4 ‑1.9 ‑0.6143intergenic (‑163/+129)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (82/55):  total (87/56)
Fisher's exact test strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.63e-01

Only 100 of 196 total aligned reads displayed.

GATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGT  >  NC_014034/3068080‑3068280
                                                                                                       |                                                                                                          
gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCAt                                                                                                            <  1:1533616/101‑1 (MQ=255)
gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCAt                                                                                                            >  1:1475925/1‑101 (MQ=255)
gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCAt                                                                                                            >  2:2366800/1‑101 (MQ=255)
 aggggCGGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  1:1774654/99‑1 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  1:1729808/101‑1 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  2:1111439/101‑1 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           <  1:1065946/101‑1 (MQ=255)
 aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATg                                                                                                           >  2:2827143/1‑101 (MQ=255)
   gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGTCCCGCCCATggc                                                                                                         <  1:2289102/101‑1 (MQ=255)
   gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc                                                                                                         <  1:122909/101‑1 (MQ=255)
   gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc                                                                                                         <  2:3984774/101‑1 (MQ=255)
    ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.g                                                                                                       >  1:1920046/1‑101 (MQ=255)
    ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.g                                                                                                       >  2:3773314/1‑101 (MQ=255)
     gCAGGCTGACCGAGGCCATCGCCCAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gg                                                                                                      >  2:744042/1‑101 (MQ=255)
       aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gggg                                                                                                    >  2:2595586/1‑100 (MQ=255)
       aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gggc                                                                                                    >  2:1473617/1‑101 (MQ=255)
       aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.gggc                                                                                                    >  2:1373872/1‑101 (MQ=255)
        ggCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATggc.ggggg                                                                                                   >  1:1984297/1‑99 (MQ=255)
         gCTGACCGAGGCCATCGCCGAGGTCATGCGGAATAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGgcg.c                                                                                                 <  1:3606332/101‑1 (MQ=255)
           tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.Ctt                                                                                               <  2:3120389/101‑1 (MQ=255)
           tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCACGGC.GGggg.cgt                                                                                               >  2:2022118/1‑99 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtct                                                                                             <  1:3868690/101‑1 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCAGGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtct                                                                                             <  2:4218580/101‑1 (MQ=255)
              ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtctc                                                                                            >  1:904195/1‑101 (MQ=255)
               cGAGGCCATCGCCGAGGTCATGCGGAACATCAGCCCGGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTtctcc                                                                                           >  1:3292611/1‑100 (MQ=11)
                 aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTcgc.c                                                                                        >  1:604899/1‑101 (MQ=255)
                 aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTccc.c                                                                                        >  1:227116/1‑101 (MQ=255)
                    ccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCCCCCCCCCCCTGGG.GGGCG.GGTCTcgc.cccc                                                                                     >  1:1173776/1‑99 (MQ=18)
                     cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTcgc.cc.g.cc                                                                                  <  2:2664845/101‑1 (MQ=255)
                        cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCCC.CC.G.CCcgg                                                                               >  2:2306011/1‑101 (MQ=255)
                           cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCCCCCATGGC.GGGCG.CTTCGCCC.CC.T.GCgggggg                                                                            >  1:758458/1‑99 (MQ=17)
                             aGGTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGCGGGGGG.CGCCGCGC.CC.C.CCGgggcgg                                                                           >  1:3707408/1‑101 (MQ=18)
                              ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGgggcgggc                                                                         <  2:1833609/101‑1 (MQ=255)
                               gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGggcgggcg                                                                        <  2:2439155/101‑1 (MQ=255)
                                 cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGGG.CGTCTCCC.CC.G.CCCgggcggggggc                                                                      >  1:2080720/1‑97 (MQ=18)
                                 cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGGGcc                                                                      >  2:3126655/1‑101 (MQ=255)
                                  aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGGGCCg                                                                     >  1:2382047/1‑101 (MQ=255)
                                    gcgcgaCAATGGCCCCGTGACCGGCGAGGGCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGGGCCGGCCCCC.CC.G.CCGGGGGGGGGGGCgg                                                                    >  2:1595751/6‑101 (MQ=17)
                                    gCGGACCAGTGGCCTCGTGCCCCCCGAGTGCCCAAAGC.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGc                                                                   <  1:1685015/101‑1 (MQ=17)
                                    gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGG.CC.G.CCGGGGGGGGCGCCgga                                                                   >  2:1235606/1‑100 (MQ=255)
                                     cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCCGcc                                                                  >  2:1378869/1‑101 (MQ=255)
                                     cGGAACAGTGCCCTCGTGACCGCCGAGGGCCGAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGcc                                                                  <  1:3462105/101‑1 (MQ=255)
                                      ggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCCGGGCGGGCGGCGGccc                                                                 >  2:2425929/1‑100 (MQ=255)
                                         acgggggCCTCGGGCCCGCCGAGGCCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCTGGGCGGGCGCCGGCCTctg                                                              <  2:2195308/96‑1 (MQ=14)
                                         acatggcccccGTGCCCGCGGAGGGCCAAAAGG.CCCTC.GGCCAGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTctg                                                              <  2:898105/91‑1 (MQ=11)
                                         aCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGGGCCGGCCTctg                                                              >  2:1095190/1‑101 (MQ=255)
                                         aCAATGGCCCCGTGACCGGCGAGGGCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGGG.C.GGGCGCGCC.G.CCGGGGCGGGGGGCgggggcct                                                              >  1:1355827/1‑95 (MQ=17)
                                          cAGTGGCCTCGTGACCGCCGAAGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCCC.CC.C.CCGGGGGGGGGGcccggccccgc                                                             >  1:2402932/1‑92 (MQ=14)
                                          cAGTGCCCTCGGGCCCCCCGAGGCCCCAAGGC.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTctgc                                                             <  2:1016424/101‑1 (MQ=14)
                                             tGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCtgctgc                                                          >  2:251806/1‑101 (MQ=255)
                                                 cTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGTGCCGGCCCCCGCTTCGccc                                                      >  1:98066/1‑100 (MQ=14)
                                                 cTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCCGCCTCTGCTGCGCC.t                                                     >  2:3459419/1‑101 (MQ=255)
                                                 cTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CCTCTCGC.CC.G.CCGGGGCGGGCGCCGGGCCCTGCTGCGCC.t                                                     >  2:533275/1‑101 (MQ=255)
                                                   cgggcccgccgGGGGCCCAAGGG.CCCTC.GGCCAGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.Tgg                                                   <  2:4160271/96‑1 (MQ=14)
                                                   cGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.C.CCGGGGGGGGGGCCGGCCTCTGCTGCGCC.Tgg                                                   >  2:2699520/1‑101 (MQ=255)
                                                     tgcccgccgAGGGCCCAAGGG.CCCCC.GGCCGGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.Tgggg                                                 <  2:3292370/98‑1 (MQ=14)
                                                     tGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CGTCTCGC.CC.C.CCGGGGGGGGGGCCCGGCTCTGCCGCGGC.Cgggg                                                 >  2:2685478/1‑101 (MQ=14)
                                                        ccgcggGGGCCCCAAGGC.CCCTG.GGCCCGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCCC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGaaa                                              <  1:2903314/101‑1 (MQ=14)
                                                        ccgccgGGGGCCCAAAGG.CCCCC.GGCCAGGC.CCCGCCCCGCCCATGGC.GGGCC.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGaaa                                              <  1:2784782/101‑1 (MQ=14)
                                                         cgccgAGGGCCCAAGGC.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAAc                                             <  1:1826126/101‑1 (MQ=255)
                                                         cccccggggCCCAAACG.CCCCCGGGCCGGCC.CCCGCCCCGCCCTGGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGaaa                                              <  2:1900966/95‑1 (MQ=14)
                                                            cgAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGGGCCGGCCTCTGCTGCGCC.CGGGGGAACggg                                          >  1:1096149/1‑100 (MQ=255)
                                                            cgAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCCCTGCTGCGCC.TGGGGGAACggg                                          >  1:1454992/1‑100 (MQ=255)
                                                             gAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGGGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                         >  2:3358883/1‑101 (MQ=255)
                                                             gAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGGAACGGTc                                         >  1:4051850/1‑101 (MQ=255)
                                                             gAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCCCTGCTGCGCC.CGGGGGAACgggc                                         >  1:2987262/1‑99 (MQ=255)
                                                              ggggCCCCAAGGCCCCCC.GCCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                         <  1:2366976/100‑1 (MQ=17)
                                                              gaggCCCAAAGGCCCCTC.GGCCGGCC.CCCGNCCCGCCCAGGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                         <  1:195563/99‑1 (MQ=17)
                                                              gaggCCCAAAGG.CCCCCGGGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTc                                         <  2:2545566/99‑1 (MQ=17)
                                                              aGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGGAACGGGCt                                        >  1:2505581/1‑101 (MQ=255)
                                                               gggCCCAAGGG.CCCCC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCtt                                       <  2:2006297/101‑1 (MQ=255)
                                                               gggCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGCG.CGGTCCGC.CCGG.CCCGGGCGGGGGGGGGCCCCCGCCGTGCCTTGGGGGAACgggc                                         >  1:1114979/1‑99 (MQ=2)
                                                                ggCCCAAAGG.CCCTC.GGCCTGCG.CCCGCCCCGCCATCGGC.GGGCC.CTGGCCGC.GC.GCCCGGGGGGGGCGCCCGCCCCTTCTCCCGC.Tggggtgatcgggtc                                       >  2:2935939/1‑91 (MQ=2)
                                                                  gccAAAGT.CCCCT.GGCCCGCCCCCCGCCCCGCCCATGGC.GGGCG.CTTCGCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTgg                                     <  2:4130183/100‑1 (MQ=14)
                                                                     agcgg.CCCCC.GGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCAcc                                 <  1:4228933/98‑1 (MQ=18)
                                                                      aaGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCCC.CC.G.CCCGGGCGGGGGGCCGCCTCTGCTGCGGC.TTGGGGAACGGGCTTGGCACCg                                >  2:3871726/1‑101 (MQ=17)
                                                                       aggccccTC.GGCCGGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CGGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCg                                <  1:4151015/97‑1 (MQ=18)
                                                                       aGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.CGGGGAAACGGTCTTGGCCCCGc                               >  1:4187860/1‑101 (MQ=255)
                                                                         g.CCCTC.GCCCAGCC.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGccc                             <  2:2146985/101‑1 (MQ=255)
                                                                               c.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGGGCCGGCCCCCGCTGCGCC.TGGGGGAACGGGCTTGGCACCGCCCGGGcc                        >  1:2878136/1‑100 (MQ=18)
                                                                               c.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGAACCCGCCTCTGCGGCGGC.TGGGGAAAAGGTGTTGGCACCCCCCGGGcc                        >  1:4005573/1‑100 (MQ=18)
                                                                                 ggCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGGAACGGTCTTGGCACCGCCCGGGCTc                       >  1:2390801/1‑101 (MQ=255)
                                                                                 ggCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTc                       >  2:3260323/1‑101 (MQ=255)
                                                                                    cAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCcgg                    >  1:1311981/1‑101 (MQ=255)
                                                                                      gcg.cCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCcggcg                  >  2:2786053/1‑101 (MQ=255)
                                                                                        g.cccgcccCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAANCGGTCTTGGCACCGCCCGGGCTCCggcggg                >  1:1057335/1‑101 (MQ=255)
                                                                                        g.cccgcccCGCCCATGGC.GGGCG.CTTCTCCC.CC.G.CCGGGGCGGGCGGCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCCCCGCCCGGGCTCCggcggg                >  2:3535763/1‑101 (MQ=255)
                                                                                            ccccccgcccAGGGG.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg             <  1:2727216/99‑1 (MQ=255)
                                                                                             gccccgcccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGcgggcggg            >  1:257813/1‑101 (MQ=255)
                                                                                               ccggcccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa          <  1:711033/98‑1 (MQ=255)
                                                                                                ccgcccGTGGC.GGGCG.TTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa         <  2:565013/101‑1 (MQ=255)
                                                                                                ccgcccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCCGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa         >  1:3964315/1‑101 (MQ=255)
                                                                                                  gcccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAg       >  1:3348522/1‑101 (MQ=255)
                                                                                                    ccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc     >  1:1381241/1‑101 (MQ=255)
                                                                                                    ccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAAGc     >  2:2823067/1‑101 (MQ=255)
                                                                                                    ccATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGGCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGc     >  1:1493012/1‑101 (MQ=255)
                                                                                                    ccAGGGC.GGGCG.CTTCTCGC.CC.G.CGGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGc     <  1:2450913/101‑1 (MQ=255)
                                                                                                      gtGGC.GGGCT.CTCCTCGC.CC.G.GCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAg   <  2:2080582/100‑1 (MQ=255)
                                                                                                      aTGGC.GCGCG.CTTCTCGC.GC.G.CCCGGGCGGCGACCGGGCCTTGTTGCACC.TGGGGTAACGGTCTTTGCGCCCCCTAGGCTCCGGCGGGCggggccagctag   >  2:685563/1‑93 (MQ=255)
                                                                                                       tGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAgg  >  2:3920689/1‑100 (MQ=255)
                                                                                                       |                                                                                                          
GATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGG.CCCTC.GGCCAGCG.CCCGCCCCGCCCATGGC.GGGCG.CTTCTCGC.CC.G.CCGGGGCGGGCGCCGGCCTCTGCTGCGCC.TGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAGT  >  NC_014034/3068080‑3068280

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG